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Biomedical subjects

G Reiss

Publications and source records attributed to G Reiss.

At least 109 records · Page 6Linked to original sources

[Dealing with the scientific literature--a report of experiences].

There are several problems in collecting, analysing and receiving medical articles or literature. The increasing number of medical articles calls for a computer-aided system for literature data base programs. Therefore we want to present our own experiences. The computer-aided data bases such as MEDLINE and EMBASE are very useful but many other difficulties regarding citation and procurement of articles remain.

Databases, Bibliographic↗

[Experience with the reference manager EndNote-EndLink].

A good reference management program should make it easy to record the elements of a reference: author's name, year of publication, title of article, etc. It should offer tools that let you find and retrieve references quickly, and it should be able to produce the bibliography in the format required for a particular publication. There are many computer programs, but very few stand out as truly useful, time saving, and work enhancing. One of them is EndNote-EndLink. We want to report our experience with this database manager. The functions and the use of the software package EndNote 2.3 for Windows are described. You can create your database or you can download batches of references from one of the popular searching services (e.g. MEDLINE). When you want to cite a reference you simply paste the reference wherever you want your in-text citation to appear. To prepare the bibliography, EndNote scans your article, replaces the place holders with citations and prints the list of references at the end of the manuscript, according with the style that you have chosen. Altogether EndNote provides an excellent combination of features and ease of use.

Bibliographies as Topic↗

Synthesis of articular cartilage-like tissue in vitro.

Defects in mature articular cartilage do not heal without residues, and therefore they remain a challenging problem in orthopaedic surgery. Modern tissue culture techniques facilitate the synthesis of cartilage-like tissue. A requirement of retaining the phenotypic characteristics of chondrocytes in vitro is the use of three-dimensional culture techniques. Articular chondrocytes of adult rabbits were isolated and cultured on different transplantable media for several weeks. A resorbable fleece, a non-absorbable net and lyophilized dura were used. Viability was tested by immunohistochemical techniques. Deposition of extracellular matrix could be observed by electron microscopy. The phenotypical and morphological appearance of cultured chondrocytes was preserved on the resorbable polymer fleece and the lyophilized dura. Cells cultured on the non-absorbable net had a more fibroblastic appearance. The resorbable fleece is apparently most suitable in terms of viability of the cultured chondrocytes and biocompatibility. The cultured three-dimensional artificial cartilage constructs reveal a future possibility for autologous cartilage transplantation into mature cartilage defects.

Animals↗

Intraoperative facial nerve monitoring (IFNM) predicts facial nerve outcome after resection of vestibular schwannoma.

Intraoperative facial nerve monitoring (IFNM) is a suitable technique for intraoperative facial nerve identification and dissection, especially in large vestibular schwannomas (VS) (acoustic neuroma). To evaluate its feasibility for estimating functional nerve outcome after VS resection 60 patients underwent surgery using IFNM. Out of this group the last 40 patients were included in a prospective study evaluating the prognostic value of various IFNM parameters (proximal and distal absolute EMG amplitude, stimulation threshold, and proximal-to-distal amplitude ratio) for prediction of initial postoperative facial nerve function and recovery of function. Stimulation threshold and absolute EMG amplitude proximally at the brain stem were both predictive for postoperative nerve function. Good initial facial nerve outcome (modified House Brackmann grading, mHB degree I and degree II) was found in 15/16 patients with a proximal EMG amplitude greater than 800 microV and in 19/22 patients with proximal stimulation threshold less than 0.3 mA. Sixteen of 16 patients with proximal stimulation threshold equal to or greater than 0.3 mA had moderate-to-severe facial palsy (mHB degree III or worse). Six of six patients without evokable proximal amplitude initially had insufficient nerve function (mHB degree IV). Intraoperative decrease of the proximal amplitude was associated with an unfavourable outcome, whereas distal amplitudes usually stayed unchanged. Mean distal EMG amplitudes were also found to be decreased with poor nerve function, which may mean that the tumour had already affected the nerve. A proximal amplitude of 300 microV or less and a proximal-to-distal amplitude ratio below 1:3 were found in the absence of functional recovery in 6/8 (75%) and 5/6 (83%) patients with initial mHB degree IV, respectively. Two patients with initial mHB degree IV improved to mHB degree III despite intraoperative evidence of missing functional nerve integrity. Therefore, functional recovery cannot be predicted by IFNM in all cases of anatomical nerve preservation. We conclude that a minimum follow-up period of 1 year may still be advisable even in certain patients without evidence of intraoperative functional nerve integrity.

Adult↗

Some aspects of earedness--the validity and reliability of self-report items.

In contrast to the extensive literature on hand, foot, and eye preferences there are only a few studies concerning ear preference. From a review of 27 studies 13 items (Stopwatch, Telephone receiver, Tablecloth, Head movement, Wall, Drawer, Wall phone, Heartbeat, Door, Earphone, Box, Pocket radio, Voice) were identified in the measurements of ear preference. In a series of investigations, these self-report items were validated with respect to individual behavioural measures and test-retest reliabilities were obtained over 1 mo. and 1 yr. for earedness. These data indicate that a questionnaire composed of these items could be a valid and reliable method for the assessment of various forms of ear preference, but there are several differences among the items in validity and reliability.

Adult↗

Ear preference: association with other functional asymmetries of the ears.

The bases for the preferential use of one ear are not clear. We investigated ear preference, asymmetry on a dichotic listening test and acuity dominance (laterality of hearing loss) in a sample of 51 patients with mild high frequency hardness of hearing. There was a higher correlation between measured ear preference and the measured acuity dominance than between ear preference and dichotic listening, suggesting a certain peripheral (cochlear) base for the ear preference.

Auditory Perception↗

Some aspects of self-reported hand preference.

The aim of the present study was to compare self-reported handedness with the directly tested hand preference using a 10-item battery in sample of 1223 students. The question assessing self-reported handedness was whether you are a left- or a right-hander or you use both hands equally. Responses indicate that self-reported handedness is a crude but possible method of measurement. Female right-handers showed the best agreement.

Adult↗

Signs of latent handedness in families.

Luria argued that several behaviors suggested a "latent" handedness. He suggested that such things as the way in which one crossed one's arms or clasped one's hands might reflect a latent preference for the left hand. Arm-folding refers to the preferential tendency for individuals to fold one forearm over the other, whereas hand-clasping refers to the preferential tendency for individuals to clasp the hands together. We investigated hand-clasping and arm-folding in 292 families (mother, father, and offspring). In this study about 55% of the population are left-hand-claspers, 44% are right-hand-claspers, and the remaining 1% report that they have no preference or are indifferent. About 54% of the population are left-arm-folders, 42% are right-arm-folders, and the remaining 4% report that they have no preference or are indifferent. Familial data suggest that hand-clasping and arm-folding may be under genetic control: although the data do not fit any straightforward recessive or dominant Mendelian model, they are compatible with the type of model invoking fluctuating asymmetry which has been used to explain the inheritance of handedness. It is possible that hand-clasping and arm-folding as well as leg-crossing may be idiosyncrasies due to or influenced by physical bilateral differences in the hands or arms. All family data including others and mine together (arithmetical sum) suggest a genetic contribution, although environmental influences are also evident.

Adult↗

[Several aspects of modern literature research].

This article reviews today's methods of searching the literature, especially the electronic ones. There are 2 main methods of searching: CD-ROM- and online searching. Online searching is a fast and powerful alternative to CD-ROM searching. Formal training is necessary for quick and cheap reference finding. Other benefits such as direct transfer from online searching to your own computer bibliography database make online searching of biomedical databases essential to consider.

CD-ROM↗

[Selected aspects of computer-assisted literature management].

We want to report about our own experiences with a database manager. Bibliography database managers are used to manage information resources: specifically, to maintain a database to references and create bibliographies and reference lists for written works. A database manager allows to enter summary information (record) for articles, book sections, books, dissertations, conference proceedings, and so on. Other features that may be included in a database manager include the ability to import references from different sources, such as MEDLINE. The word processing components allow to generate reference list and bibliographies in a variety of different styles, generates a reference list from a word processor manuscript. The function and the use of the software package EndNote 2 for Windows are described. Its advantages in fulfilling different requirements for the citation style and the sort order of reference lists are emphasized.

Database Management Systems↗

A specific screen for oligosaccharyltransferase mutations identifies the 9 kDa OST5 protein required for optimal activity in vivo and in vitro.

The central reaction in the process of N-linked protein glycosylation in eukaryotic cells, the transfer of the oligosaccharide Glc(3)Man(9)GlcNAc(2) from the lipid dolicholpyrophosphate to selected asparagine residues, is catalyzed by the oligosaccharyltransferase (OTase). This enzyme consists of multiple subunits; however, purification of the complex has revealed different results with respect to its protein composition. To determine how many different loci are required for OTase activity in vivo, we performed a novel, specific screen for mutants with altered OTase activity. Based on the synthetic lethal phenotype of OTase mutants in combination with a deficiency of dolicholphosphoglucose biosynthesis which results in non-glucosylated lipid-linked oligosaccharide, we identified seven complementation groups with decreased OTase activity. Beside the known OTase loci, STT3, OST1, WBP1, OST3, SWP1 and OST2, a novel locus, OST5, was identified. OST5 is an intron-containing gene encoding a putative membrane protein of 9.5 kDa present in highly purified OTase preparations. OST5 protein is not essential for growth but its depletion results in a reduced OTase activity. Suppression of an ost1 mutation by overexpression of OST5 indicates that this small membrane protein directly interacts with other OTase components, most likely with Ost1p. A strong genetic interaction with a stt3 mutation implies a role in complex assembly.

Amino Acid Sequence↗

Medial meniscus replacement by a fat pad autograft. An experimental study in sheep.

The medial meniscus in 15 sheep was replaced by a pediculated infrapatellar fat pad graft and resulted in the development of a macroscopically meniscus-like structure within 6 months. Five additional sheep with a meniscectomy were controls. Degenerative changes in the fat pad autograft were visible after one year. Osteoarthritis of the weightbearing medial compartment was detected after 6 months. A temporary protective effect on the cartilage could be attributed to the autograft, but the long term results indicated that this was not permanent. Fat is not suitable as a meniscal substitute.

Adipose Tissue↗

The STT3 protein is a component of the yeast oligosaccharyltransferase complex.

N-linked protein glycosylation is an essential process in eukaryotic cells. In the central reaction, the oligosaccharyltransferase (OTase) catalyzes the transfer of the oligosaccharide Glc3Man9GlcNac2 from dolicholpyrophosphate onto asparagine residues of nascent polypeptide chains in the lumen of the endoplasmic reticulum. The product of the essential gene STT3 is required for OTase activity in vivo, but is not present in highly purified OTase preparations. Using affinity purification of a tagged Stt3 protein, we now demonstrate that other components of the OTase complex, namely Ost1p, Wbp1p and Swp1p, specifically co-purify with the Stt3 protein. In addition, different conditional stt3 alleles can be suppressed by overexpression of either OST3 and OST4, which encode small components of the OTase complex. These genetic and biochemical data show that the highly conserved Stt3p is a component of the oligosaccharyltransferase complex.

Alleles↗

Asymmetry of the stepping test.

The influence of handedness on a measure of the vestibulospinal response was investigated with 25 right-handed and 25 left-handed subjects to examine the asymmetry of the stepping test. Right-handed subjects showed dominance of the right side indicated by direction of movement, but left-handed subjects tended much less to the left side so a functional asymmetry is for left-handers somewhat less clearly expressed.

Adult↗

Lateral preferences in a German population.

There are only a few studies in the literature concerning all four lateral preferences (handedness, footedness, eyedness, earedness) and presenting their interrelationships. Porac and Coren's 1981 inventory was used to assess hand, foot, eye, and ear preference in a German sample of 506 men and 430 women. A right sided preference was found for hand, foot, eye, and ear preference among 91%, 74%, 66%, and 63% of the sample, respectively. More men were left-handed and left-footed than women. There were no significant sex differences for eyedness and earedness. Correlations ranged from .22 between hand and eye laterality to .44 between hand and foot laterality.

Adult↗

Functional importance of hand clasping and arm folding.

The aim of the present study was to investigate the relationship between handedness and hand clasping or arm folding. Our own investigations have shown no relationship as is consistent with most results in the literature which suggests only minor functional importance of these two signs of latent handedness.

Adult↗

Further aspects of the asymmetry of the stepping test.

The test-retest reliability of the asymmetry of the Unterberger stepping test was obtained over a 3-mo. period. This stepping test is a test examining the labyrinthine deviation when testing the lower extremities. Also, self-report items were validated against individual measures of the stepping test. The investigations show high reliability of the asymmetry of the stepping test. The data indicate further that a questionnaire is a valid method for the assessment of the asymmetry of the stepping test.

Adult↗