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Biomedical subjects

G Reinhardt

Publications and source records attributed to G Reinhardt.

At least 73 records · Page 4Linked to original sources
PubMed1982

Biochemical characteristics of non-streptomycin-producing mutants of Streptomyces griseus. II. Lipids and fatty acid composition of vegetative mycelia.

Five non-streptomycin-producing non-aerial-mycelium-forming mutants (Str-Amy-) of Streptomyces griseus obtained either by spontaneous degeneration or during continuous cultivation of the high-producing aerial-mycelium-forming parent strain HP (Str+Amy+) were checked with regard to the composition of mycelial lipid material. All the Str-Amy- derivatives differed from their ancestor strain HP by an increased ration of 12-methyltetradecanoic acid (aC15:0) to isopalmitic acid (iC16:0) during growth on a chemically defined medium lacking branched-chain amino acids. This finding attests alterations in the availability of precursors for the biosynthesis of methyl-branched fatty acids. The qualitative composition of phospholipids and other polar lipids in one mutant group was found to be similar to the progenitor strain but, additionally, both a yellow pigment and a neutral lipid component were produced in excess. A second type of mutant differed by its incapability to form ornithinolipids even under phosphate limitation. Changes of phospholipid composition were demonstrated in the course of fermentation. Formation of ornithinolipid was suppressed by an excess of inorganic phosphate in the medium, while the portions of phosphatidylethanolamine and cardiolipin increased strongly. Furthermore, the formation of ornithinolipids was influenced by nitrogen sources. these results suggest that the composition of membrane of S. griseus varies in dependence upon the composition of the medium and the age of the mycelium.

Cell Membrane↗
PubMed1980

[Technic of venous access for infusion therapy and parenteral nutrition].

The creation of a secure venous access is the basis of the infusion therapy and the parenteral nutrition. As ways of access are suited above all the subclavian vein, the internal jugular vein and the basilic vein. Taking into consideration the contraindications and the exact performance of the various methods early complications such as punctures of the arteries, pneumothorax, rupture of the catheter and extended haematomata may be reduced to a minimum. Abnormal positions are avoided by control of the position by means of endo-ECG via steel mandrin. As late complications are observed infections at the place of puncture, unclear, partly septic temperatures and clinically manifest thromboses. Own experiences with 3,282 central venous catheters (2,057 subclavian catheters, 63 catheter into the internal jugular vein and 1,162 central catheters with access via arm veins) are taken into consideration.

Catheterization↗
PubMed1980

Isolation and structures of nitrogen-free platenolide glycosides. I. The 5-O-(deoxy-3'-C-acetyl-beta-D-hexopyranosyl)-platenolides I and II.

Four novel nitrogen-free glycosides of platenolides I and II were isolated as secondary shunt metabolites of the turimycin biosynthesis from the culture broth of an industrial strain of Streptomyces hygroscopicus IMET JA 6599. By spectral (MS, 1H and 13C NMR) studies the structures of the glycosides have been settled as 5-O-(4',6'-dideoxy-3'-C-acetyl-beta-D-hexopyranosyl)-platenolide I (DDAH-Pl-I), 5-O-(4',6'-dideoxy-3'-C-acetyl-beta-D-hexopyranosyl)-platenolide II (DDAH-Pl-II), 5-O-(4',6'-dideoxy-3'-C-acetyl-beta-D-hexopyranosyl)-14-hydroxyl-platenolide II (DDAH-OH-Pl-II) and 5-O-(6'-deoxy-3'-C-acetyl-beta-D-hexopyranosyl)-platenolide II (DAH-Pl-II). A fifth glycoside, 5-O-(6'-deoxy-3'-C-acetyl-beta-D-hexopyranosyl)-platenolide I (DAH-Pl-I) was identified through its MS data.

Anti-Bacterial Agents↗
PubMed1980

Isolation and structures of nitrogen-free platenolide glycosides. II. The 5-O-(alpha-mycarosyl)- and 5-O-(3'-demethyl-beta-mycaroxyl)-platenolides I and II.

Three novel glycosides of platenolides I and II containing either mycarose (2,6-dideoxy-3-C-methyl-L-ribohexopyranose) or 3-demethyl-mycarose (2,6-dideoxy-L-ribohexopyranose) were isolated as the shunt products of turimycin biosynthesis by an industrial strain of Streptomyces hygroscopicus IMET JA 6599. By means of MS, 13C and 1H NMR spectroscopic studies, their structures were assigned as 5-O-(alpha-mycarosyl)-platenolide I (MYC-Pl-I), 5-O-(alpha-mycarosyl)-platenolide II (MYC-Pl-II) and 5-O-(3'-demethyl-beta-mycarosyl)-platenolide II (DM-MYC-Pl-II). The occurrence of 3-demethyl-mycaroside amongst the shunt metabolites is discussed in terms of its biosynthesis.

Acetylation↗
PubMed1980

[The role of the scout-film in urography (author's transl)].

The importance of the scout film (plain film of the abdomen) prior to any contrast examination of the urinary tract is pointed out. After initial review of radiographic techniques and normal anatomic findings, the pathologic findings due to urinary tract disease are pointed out and are differentiated from those of non urinary--tract origin.

Diagnosis, Differential↗