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Biomedical subjects

G Prasad

Publications and source records attributed to G Prasad.

41 records · Page 3Linked to original sources
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VP2 gene based phylogenetic relationship of Indian isolates of Bluetongue virus serotype 1 and other serotypes from different parts of the world.

Bluetongue virus (BTV), a member of genus Orbivirus, family Reoviridae, is non-enveloped with double shelled structure and 10 segmented double stranded RNA genome. The RNA segment L2 encodes an outer capsid serotype specific viral protein VP2. BTV serotype 1 (BTV-1) specific novel primer pair, forward primer (1240-1271 bp) and reverse primer (1844-1813 bp), was designed using VP2 gene sequences available in GenBank to amplify 1240-1844 bp region because two hypervariable and three conserved regions have been reported within these 604 nucleotides. This primer pair successfully amplified cell culture adapted six Indian isolates of BTV-1. The 604 bp PCR product of VP2 gene of BTV-1 Avikanagar (A), Chennai (C) and Sirsa 3 (S3) Indian isolates were cloned in pPCR-Script Amp SK (+) vector and transformed into XL10-Gold Kan ultracompetent Epicurian coli cells. The positive clones selected by blue-white screening and colony touch PCR were sequenced. BTV-1A, C and S3 isolates revealed 99% nucleotide sequence identity within 1304-1844 bp region of VP2 gene. The partial VP2 gene sequences (1240-1844 bp region) revealed that BTV-1 Indian isolates were 89% identical with Australian (AUS) BTV-1 isolates while the identity with South African (SA) BTV-1 isolate was 75%. Phylogenetically, three BTV-1 Indian isolates formed one group which is closely related to BTV-1AUS isolates followed by BTV-1SA, BTV-2, 9, 23, 13, 17, 10 and 11 isolates from different parts of world. Based on partial VP2 gene sequences, it is concluded that Indian isolates of BTV-1 are closely related to BTV-1AUS isolates than BTV-1SA and other serotypes.

Animals↗
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Soil as a possible source for the severe fruit rot of guava (Psidium Guajava l.) due to Phytophthora Nicotianae var. parasitica (Dastur) Wat. at Kurukshetra (India).

Severe fruit rot of guava due to Phytophthora nicotianae var. parasitica (syn. P. parasitica Dastur sensu stricto) was observed during the months of July and August in 1976 at Kurukshetra (India). The pathogen was successfully isolated from the soil below the infected fruit trees using ripe guava fruits as baits as well as by using a selective medium at regular intervals for a period of one year. Presence of the pathogen in the soil below the guava trees and its isolation from the soil for a period of one year, i. e. the next fruiting season of guava trees indicated that perhaps the soil is the main source of infection.

Fruit↗