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Biomedical subjects

G Otto

Publications and source records attributed to G Otto.

At least 235 records · Page 13Linked to original sources
PubMed1987

Hematuria in rabbits.

A nine year retrospective study of hematuria in 14 New Zealand White rabbits was conducted to classify possible etiologies of this clinical finding. Physical examination, laboratory tests, radiography and postmortem examination were utilized in most cases to verify the presence of hematuria and to determine its etiology. Uterine adenocarcinoma was diagnosed in two rabbits. Three rabbits had uterine polyps with hemorrhage. Renal infarction with hemorrhage was diagnosed in three rabbits. Urolithiasis with secondary urethral obstruction and hemorrhagic cystitis was identified as the cause of hematuria in four rabbits. Other causes of hematuria included chronic cystitis, disseminated intravascular coagulation, bladder polyps and pyelonephritis. Hematuria of undetermined origin was observed in one rabbit. This last [corrected] case was negative for both blood and porphyrin in the urine, but positive for excess levels of urobilin, the oxidative product of urobilinogen. This case illustrates that hyperpigmented urine should be a rule out in all cases of suspected hematuria in rabbits.

Adenocarcinoma↗
PubMed1986

[Current status of liver transplantation].

The importance of the liver transplantation for the treatment of infaust liver diseases is nowadays regarded as undisputed. The results could be improved during the last years: on an average 40% of the patients still live one year after the transplantation. The control of the transplant rejection and the diminution of the ischaemic liver damage are preeminent pathophysiological problems. Clinical routine and experience essentially further the optimization of the results.

Common Bile Duct↗
PubMed1986

Preservation damage in liver transplantation. Influence of rapid cooling.

Liver preservation and transplantation was performed in pigs. For flush perfusion cold (2 degrees C) and warm (15 degrees C) lactated Ringer's solution were compared. Administration of cold Ringer's resulted in a severe endothelial damage of sinusoids that was aggravated after recirculation, as shown by electron microscopic examination. Using warm Ringer's solution this alteration was limited. Liver parenchymal cells are impaired mainly during reflow as a consequence of microcirculatory disturbance. After cold flushing animals died within a few hours, whereas after flushing with warm Ringer's solution pigs survived definitively.

Animals↗
PubMed1985

Alteration of the ultrastructure of the pig liver after a continuous perfusion of six hours in vitro.

Livers of mini LEWE pigs were perfused continuously for 6 h on a novel perfusion system through the portal vein (laminar flow from 200 to 300 ml/min, pressure 270 Pa) and through the A. hepatica (pulsating flow from 100 to 200 ml/min, pressure: 10.6/5.5 kPa). The following perfusion media were used for determining the best conservation conditions at 10 to 12 degrees C: Pig blood/Krebs-Henseleit bicarbonate buffer 1:1; Pig blood/Ringer lactate solution 1:1; Pig plasma; Collins C 2 perfusion solution. After perfusion with Collins C 2 solution the hepatocytes show swelling and dilation of the granular endoplasmic reticulum; after perfusion with the other solutions the changes of the hepatocytes are negligible. The alterations of the sinusoidal wall cells depend on the perfusion pressure and flow and the composition of the solution. After continuous perfusion the sinusoidal walls are characterized by vesicles of endothelial cells, dilatation of the Disse space and fragmentation of hepatic microvilli.

Animals↗
PubMed1985

[Clinical liver transplantation in East Germany].

In the GDR 28 liver transplantations have been performed up to now. The 1 year survival rate amounts to 30%. At present 8 patients are alive between 3 months and 5 years. There were no biliary complications since the biliary reconstruction was performed by using a special 'mucosa suture'-technique. Ischaemic graft failure was a main cause for early postoperative mortality. A better immunosuppressive treatment may help to improve the results of liver transplantation in future.

Adult↗
PubMed1984

[Value of histopathologic findings following liver transplantation based on biopsy course studies].

A total of forty-four biopsies from nine liver transplantation patients, who had developed complications postoperatively, were studied histologically and, in part, electron-microscopically. Morphologic signs of acute rejection were seen in three patients, while there were indications of chronic rejection in one patient. In combined lesions, such as drug-induced hepatosis, cholangitis, hepatitis, and rejections, the interpretation of biopsy findings may be difficult, and special entities cannot in every case be separated unequivocally. Changes of serum bilirubin and of serum enzyme activity do not run entirely parallel to the microscopically detectable structural lesions. For diagnosis of complications after liver transplantation both the morphologic and the clinical findings must be considered.

Adult↗
PubMed1984

Adenine nucleotides and glycolysis during liver preservation by simple hypothermic storage.

Adenine nucleotides and glycolytic metabolites were examined during hypothermic storage of porcine livers by different preservation methods. The parameters ATP, ADP, AMP, G6P, F6P, FDP, GAP, 2PG, PEP, Pyr and Lact were investigated. The results indicate a dependence of adenine nucleotides but no dependence of glycolytic metabolites on preservation method. Storage by plasma protein fraction or Collins C2 solution causes a better result in TAN concentration after 6 h than albumin and Ringer storage or Ringer trickle perfusion. Conditioning the organ for a better outcome of preservation aimed at increasing the glycolytic rate is of questionable value.

Adenine Nucleotides↗
PubMed1984

[2-Cuff technic for experimental liver transplantation in pigs].

A transplantation model for the experimental liver transplantation is introduced that forms the damage of the liver during the transplantation constantly and therewith reproducibly. The cuff technique was adapted for liver transplantation in pig. The infrahepatic vena cava and the portal vein were anastomosed by cuff technique, the suprahepatic vena cava by continuous suture technique. The demonstrated experiments include 34 cuff transplantations. The complication rate amounted to 14.7%. This technique is proved in our animal experimental practice.

Animals↗
PubMed1983

[A method for determining the hemodynamic behavior of the liver in vitro].

Hemodynamic parameters of livers--especially resistance of the sinusoids and of functional portosystemic shunts--are investigated using an electro-physical model. During liver perfusion the inflow conduit is compressed irregularly. The dynamic changes of pressure and flow allow a calculation of the volume passing the sinusoids and the functional shunts.

Animals↗
PubMed1983

[Modification of hemodynamic parameters of the liver by preservation].

Perfusion of sinusoids and of functional portosystemic shunts was investigated before and after PPF-, Collins- and Ringer storage and in Ringer-trickle-perfusion of the liver using a physical-mathematical method. The increase of the sinusoidal resistance after PPF-storage is less than after preservation by electrolyte solutions. Accordingly the amount of volume passing the sinusoids is higher after PPF-storage. This fact is of great importance for haemodynamic integrity after transplantation.

Animals↗
PubMed1983

[Status and perspectives of liver transplantation].

Liver transplantation is indicated in infaust liver damages only. The most important complications are rejection, disturbances in the bile tract, problems with preservation and general infections. In the surgical clinic of the Charité Berlin 17 liver transplantations have been practiced. 5 of those patients are living now between 2 months and 3.3 years. A new technique of bile duct reconstruction was tested and is clinically used now. Diagnosis of rejection and of liver preservation are subjects of our own scientific work.

Bile Ducts↗