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Biomedical subjects

G Moreno

Publications and source records attributed to G Moreno.

At least 109 records · Page 6Linked to original sources
PubMed1976

Evaluation of the gastrocolic space in 100 cases of acute pancreatitis.

The frequency of the main radiological signs found in plain films of the abdomen is presented in 100 cases of acute pancreatitis. The authors believe that this is the first statistical analysis of the gastrocolic separation sign. It is most useful to recognize the gastrocolic sign because of its high incidence (49%) and to understand the physio-pathological alterations that contribute to its formation.

Acute Disease↗
PubMed1976

Studies on guinea pig skin cell cultures. V. Co-culture of pigmented melanocytes and albino keratinocytes, a model for the study of pigment transfer.

Mixed cultures of melanocytes (M) and keratinocytes (K) are easily obtained from pigmented guinea pig ear skin. They are suitable for the study of pigment transfer from M to K. However, quantitation is difficult because many K are already loaded with pigment prior to cultivation. A technique is presented in which pigment-producing M are co-cultured with K of albino origin. Pigmented guinea pig ear skin is split with trypsin and basal cells including M are harvested. The cell suspension is treated with sodium citrate which prevents the attachment of K (but not of M) to the culture substrate. Ninety per cent pure M cultures are obtained. Five to seven days later, another basal cell suspension is prepared, this time from albino ear skin. This second suspension is seeded on top of the pigment-forming culture of M. The number of contacts between albino K and pigment-forming M increases as a direct function of time. Contrarily, the number of K which become pigmented increases until the fifth day of co-culture and decreases thereafter.

Animals↗
PubMed1975

[Blood cultures-bacteriological and drug resistance culture (author's transl)].

Blood cultures of 24 patients from the Hospital das Clinicas da Faculdade de ciências Médicas e Biológicas de Botucatu showed a prevalence of gram-negative isolates (83%) over gram-positive (16%). Among the microorganisms Salmonella typhimurium was the most frequent (25%) followed by Klepsiella sp. (21%) and Escherichia coli (17%). It was also observed that the main cuase of death was the largest occurrence of Salmonella typhimurium and Pseudomonas aeruginosa among the isolates. Regarding drug resistance gentamycin revealed more active than other antibiotic tested.

Anti-Bacterial Agents↗
PubMed1975

[Contribution to the study of the haemolytic streptococci isolated from dogs (author's transl)].

The authors studied bacteriologically 740 specimens from dogs maintained at the central kennel of the Faculdade de Ciências Médicas e Biológicas de Botucatu, being the main subject the isolation of streptococci strains from the tonsilar, vaginal conjuctival and preputial secretions. Strains with the same serological properties showed a different biochemical properties, which allowed us to appoint different biochemical types inside a same serological group. On the other hand, it was also possible to detect through the precipitation capillary tube technique, the serological groups G, C, A, D and B, being the G group (73%) the most frequent one. Biochemical properties of the human G group streptococci compared with the canine G group streptococci showed a statistically significant difference in the reactions of fibrinolysis of human plasma, glicerol, sorbitol, esculin, mannitol, inulin, raffinose, salicin, trehalos, hydrolysis of the sodium hypurate, growth on 20% bile broth, and growth at 45 degrees C. The authors stress the utility of the fibrinolysis of human plasma test for the characterization of canine origin streptococci.

Animals↗
PubMed1975

[A bacteriological study of otitis in dogs (author's transl)].

Bacteriological examination of 100 cases of otitis in dogs revealed pure cultures of different microorganisms as follows: Staphylococcus aureus from 25 animals; Escherichia coli from 10; Proteus mirabilis from 20; Enterobacter sp. from 18 and Klepsiella sp. from 7. Mixed cultures of Proteus mirabilis, Pseudomonas aeruginosa and Klepsiella sp. were found in 3 animals; Staphylococcus aureus and Enterobacter sp. in 5; Staphylococcus aureus and Proteus mirabilis in 4. Enterobacter sp. and Klepsiella sp. in 2 and Staphylococcus aureus, Proteus mirabilis and Escherichia coli in 6. Theses results indicated that Staphylococcus aureus appeared as the most frequent microorganism (36%) followed by Proteus mirabilis (23%) and Klepsiella sp. (16%). Drug resistance studies showed that all tested strains were resistant to the antibiotics and to the nalidixic acid in levels higher than 20microgram/ml except gentamycin which showed for Pseudomonas aeruginosa a resistance level for 10microgram/ml.

Animals↗