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G Mimura

Publications and source records attributed to G Mimura.

At least 37 records · Page 2Linked to original sources
PubMed1990

Immunogenetics of early-onset insulin-dependent diabetes mellitus among the Japanese: HLA, Gm, BF, GLO, and organ-specific autoantibodies--the J.D.S. study.

The Japan Diabetes Society (JDS) conducted a multicenter study on the immunogenetics of early-onset insulin-dependent diabetes mellitus (IDDM) of the Japanese. Human leukocyte antigen (HLA), properdin factor B (BF), immunoglobulin heavy-chain complex (Gm), and glyoxalase of erythrocytes (GLO) were typed, and organ-specific autoantibodies, including islet cell antibody (ICA), were assayed in 159 Japanese IDDM patients and their family members and in 258 healthy Japanese controls. The HLA-DRw9 phenotype and HLA-Bw61/DRw9 haplotype were significantly increased among the patients with autoantibodies other than ICA but with no autoimmune diseases (RR = 5.84, cP less than 0.001; and RR = 7.45, P less than 0.001), whereas the HLA-DR4 phenotype and HLA-Bw54/DR4 haplotype were significantly increased in those without either the autoantibodies or autoimmune diseases (RR = 2.64, cP less than 0.001; and RR = 4.55, P less than 0.001). The HLA-DR4 phenotype was significantly increased in the patients with autoimmune thyroid diseases (RR = 6.21, cP less than 0.05). In all groups of patients, the HLA-DR2 phenotype was significantly decreased, and the relative risk of the HLA-DRw9/DR4 genotype was highest among all HLA-DR genotypes. No significant association was found between HLA type and the duration or incidence of ICA. Gm types of g and gft were significantly increased in the patients with the autoantibodies (RR = 2.11, P less than 0.05; and RR = 34.11, P less than 0.05), whereas the BF-F phenotype was significantly decreased in the patients either with or without autoantibodies (RR = 0.43, P less than 0.05; and RR = 0.46, P less than 0.05). There was no association between IDDM and GLO type. These data indicate that immunogenetic bases underlying IDDM of the Japanese are heterogeneous, as are those in Caucasians.

Autoantibodies↗
PubMed1989

Immunogenetic heterogeneity in type 1 (insulin-dependent) diabetes among Japanese HLA antigens and organ-specific autoantibodies.

HLA phenotypes and haplotypes in relation to organ-specific autoantibody responses were studied in 82 Japanese patients with Type 1 (insulin-dependent) diabetes. HLA-DRw9 antigen and HLA phenotype of DRw9/X (X:not DR4) were increased in patients with organ-specific autoantibodies other than islet cell antibody (CP less than 0.02, RR = 4.02 and p less than 0.05 RR = 2.30, respectively); whereas HLA-DR4 antigen and HLA phenotype of DR4/X (X: not DRw9) were increased in those without the autoantibodies (CP less than 0.001, RR = 3.95 and p less than 0.01, RR = 2.46, respectively). HLA haplotype of Bw61-DRw9 was increased in patients with the autoantibodies (p less than 0.005, RR = 4.94), and HLA haplotype of Bw54-DR4 was increased in those without the autoantibodies (p less than 0.001, RR = 5.52). The relative risk of HLA-DR4/DRw9 was the highest among all HLA-DR phenotypes or genotypes in patients either with or without the autoantibodies. No association was, however, found between the incidence of islet cell antibody and HLA-DR phenotypes. These findings suggest that Type 1 diabetes among Japanese is immunogenetically heterogeneous as is Type 1 diabetes among Caucasians; and the differences in HLA-association of Type 1 diabetes among ethnic groups might give a clue to understanding of a role of HLA-antigens in the development of Type 1 diabetes.

Autoantibodies↗
PubMed1989

Effects of beta-adrenoceptor blocking agents on isolated atrial and papillary muscles from experimentally diabetic rats.

Effects of propranolol and atenolol on isoproterenol-induced responses of isolated atrial and papillary muscles from experimentally diabetic rats were examined. Male Sprague-Dawley rats were divided into the diabetic group (DM) which received streptozotocin 60 mg/kg, i.v. and the control group (C) which received vehicle i.v. At 6 weeks after, the right atrial or right ventricular papillary muscle was isolated, and the beating rate (R) in the atrium or isometric force development (F) and its first derivatives (+/- dF/dt) in the papillary muscle under pacing were recorded. Basal R was less frequent in DM than in C, but ED50 values for isoproterenol-induced chronotropy were not different between the two groups. Basal F and basal +/- dF/dt were not different between the two groups, but isoproterenol-induced increases in F and +/- dF/dt were less in DM than in C. ED50 values in F and +dF/dt were not different between the two groups. Propranolol and atenolol shifted the concentration-response curves in R, F and +/- dF/dt for isoproterenol to the right in both groups. pA2 values of propranolol and atenolol for each parameter were not different between the two groups. Results indicate that propranolol and atenolol exert the same beta-adrenoceptor blocking potency in both diabetic and non-diabetic hearts of rats.

Animals↗
PubMed1989

Glutathione S-transferases and chloroform toxicity in streptozotocin-induced diabetic rats.

The glutathione S-transferase activity in liver and kidney cytosol was significantly decreased in short term diabetes induced with streptozotocin, whereas no decrease in the transferase was observed in phenobarbital-treated diabetic rats. Toxicity of chloroform was potentiated in streptozotocin- or phenobarbital-treated rats. The decrease in liver cytosolic and microsomal glutathione S-transferase activity was observed in long term diabetic rats, and only microsomal transferase activity was restored by insulin treatment. There was no release of glutathione S-transferases into the serum in the diabetic rats, and the transferases were not inhibited by streptozotocin in vitro. These results showed that glutathione S-transferase activity decreased during diabetes, and this decrease may contribute to altering drug metabolism and toxicity in diabetes.

Alanine Transaminase↗
PubMed1989

[An autopsy case of AIDS with disseminated cytomegalovirus infection and neurological disturbance].

We report a case of acquired immunodeficiency syndrome (AIDS) complicated by disseminated CMV infection and neurological disturbance. A 21 years old male with hemophilia A was diagnosed as having AIDS in Feb. 1986 because of interstitial pneumonia and esophageal candidiasis. Since Jan. 1987 he had complained of hypesthesia in the legs. On Mar. 14 he was admitted due to diarrhea. The laboratory data revealed that WBC was 4,000/microliters including 29% of lymphocytes, 1.6% of OKT4+-, 71.6% of OKT8+-lymphocytes, T4/T8 ratio 0.02 and positive HIV antibody and HTLV-1 antibody. After the admission, sensory disturbance exacerbated to complicate paraplegia. He developed acute hepatitis associated with leukopenia, thrombocytopenia, pneumonia and melena, and eventually died on May 29. The autopsy findings disclosed CMV infection in the lungs, colons, and adrenal glands, suggesting that the primary cause of death was adrenal insufficiency. Degeneration of cerebro-spinal nerve cells and peripheral neuritis were thought to result from direct HIV infection to the nervous system.

Acquired Immunodeficiency Syndrome↗
PubMed1989

[Adult T-cell leukemia with massive melena due to marked gastrointestinal involvement].

A 60-year-old man born in Okinawa was admitted to our hospital because of epigastralgia. Physical examination revealed general lymphadenopathy, mild hepatomegaly and skin eruption. The peripheral blood leukocyte count was 168,600/microliters, with 93% abnormal lymphocytes showing convoluted or lobulated nuclei. Anti HTLV-1 antibody was positive with titer of 1: 1280 (PA). Leukemic cells had typical ATL cells' surface markers (OKT3; 97.2%, T4; 93.3%, T8; 2.8%, OKIA1; 39.6%, IL-2R; 41.8%) and complete monoclonal HTLV-1 provirus DNA. Endoscopic examination with biopsy revealed massive involvement of ATL cells into gastric mucosa. In the course of the treatment, he had extremely massive melena, and was saved by emergency operation. Multiple ulcers were found in the resected colon. Histological examination showed the marked infiltration of the ATL cells into the mucous or submucous membrane. Thereafter, he was treated well with ALG (Anti Lymphocyte Globulin), until hypercalcemia occurred. He died of acute renal failure after hypercalcemia.

Gastrointestinal Neoplasms↗
PubMed1989

Effects of dobutamine and acetylcholine on perfused hearts isolated from streptozocin-induced diabetic rats.

In order to study the responsiveness of the diabetic heart to autonomic agents, effects of dobutamine (DOB) and acetylcholine (ACh) on perfused hearts isolated from streptozocin (streptozotocin, STZ)-induced diabetic rats and insulin-treated diabetic rats were evaluated. Male Sprague-Dawley rats, weighing 180-210 g, were divided into control (C) group, diabetes mellitus (DM) group, and diabetes mellitus treated with insulin (DMI) group. C group was injected with buffered vehicle. DM and DMI groups were injected intravenously with 60 mg/kg STZ at the first day. Three days after STZ injection, DMI group was subsequently treated with 4 U of insulin zinc suspension (lente insulin) subcutaneously every day. At 45 days after injection of STZ, experiments were performed using a Langendorff perfused heart preparation. In the evaluation of effect of ACh, heart rate and myocardial developed tension (T) were measured. In the evaluation of effect of DOB, heart was paced at 300 beats/min and T was measured isometrically. Plasma glucose values (mg/dl) were 116.0 +/- 4.9 in C, 482.3 +/- 30.3 in DM, and 204.6 +/- 34.8 in DMI group, respectively. The order of percent increases in T induced by DOB (10(-8)-3 x 10(-6) g) was C greater than DMI greater than DM. The order of percent decreases in T and heart rate by ACh (10(-7)-3 x 10(-6) g) was C greater than DMI greater than DM. These results suggest that both adrenergic receptor-mediated and cholinergic receptor-mediated cardiac responsiveness are significantly depressed in the diabetic heart.

Acetylcholine↗
PubMed1988

Delayed pelvic abscess eight years after generalized peritonitis.

Recently, during a locally severe epidemic of gastroenteritis, a young woman presented, with the symptoms prevalent in her community, harboring an intraabdominal abscess. The case is presented along with a discussion of unusual and delayed presentations of abscesses in the literature. Early diagnosis is imperative because of the very significant morbidity and mortality accompanying delayed treatment.

Abdomen↗
PubMed1987

Effects of adrenergic drugs on isolated and perfused hearts of streptozotocin-induced diabetic rats.

To examine the myocardial contractile response of the diabetic heart, effects of isoproterenol (ISO) and norepinephrine (NE) on perfused hearts isolated from streptozotocin (STZ)-induced diabetic rats and insulin-treated diabetic rats were evaluated. Male Sprague-Dawley rats, weighing 200-260 g, were divided into the control (C)-group, diabetes mellitus (DM)-group and diabetes mellitus treated with insulin (DMI)-group. The C group was injected with buffered vehicle. DM and DMI groups were injected intravenously with 60 mg/kg STZ on the first day. Three days after STZ injection, the DMI group was subsequently treated with 4 U of Lente insulin subcutaneously every day. At 45 days after injection of STZ, experiments were performed using a Langendorff perfused heart preparation. The heart was paced at 300 beats/min, and myocardial developed tension (T) was measured isometrically. Plasma glucose values (mg/dl) were 142.4 +/- 8.7 in C, 499.3 +/- 15.6 in DM and 370.6 +/- 27.6 in DMI group. The order of percent increase in T induced by ISO (3 X 10(-9) - 3 X 10(-8) g) was C = DMI much greater than DM, and that by NE (10(-7) - 10(-6) g) was C greater than DMI greater than DM. On the other hand, the percent increase in T induced by CaCl2 (1.1 X 10(-4) - 2.2 X 10(-3) g) and aminophylline (AMI, 0.31 X 10(-3) - 5.00 X 10(-3) g) was not significantly different among three groups. These results indicate that adrenergic receptor-mediated contractile response was significantly depressed in the diabetic heart.

Aminophylline↗
PubMed1983

IgG heavy-chain (Gm) allotypes and HLA-antigens in insulin-dependent diabetes mellitus in Korea.

Eighty-eight patients with insulin-dependent diabetes mellitus (IDDM) and seventy-two unrelated normal controls in Korea were studied for Gm allotypes and HLA-antigens. Ten Gm phenotypes were found among the Korean population. The phenotype frequencies of Gm axg (1,2,21), Gm ag (1,21), Gm agb0b3b5st (1,21, 11,13,10,15,16) and Gm agfb0b1b3b4b5 (1,21,3,11,5,13,14,10) were higher than the other Gm allotypes, but there was no significant difference between patients and controls. HLA-BW54 was found in 15% of patients who had any of those Gm allotypes (Gm axg, Gm agb0b3b5st, Gm ab0b3b5st). However there was no significant difference in frequency as compared with controls.

Diabetes Mellitus, Type 1↗