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Biomedical subjects

G Mikuz

Publications and source records attributed to G Mikuz.

At least 109 records · Page 6Linked to original sources
PubMed1985

A comment on laryngeal cytology.

Cytology of the laryngeal mucosa proved to be a suitable method for diagnosing low-grade and intermediate dysplasia, but it did not allow any distinction between high-grade dysplasia and invasive cancer. In our experience, the use of this method should be restricted to a few conditions, such as the persistance of recurrent dysplasias and certain extended lesions from which biopsies give a high risk for scarring and permanent hoarseness.

Biopsy↗
PubMed1985

[Morphologic changes in cervix smears by intrauterine pessary].

656 cervical smears and 74 endocervical biopsies were studied for morphological alterations associated with the presence of an intrauterine device. 12,085 cervical smears served as a control for inflammatory and metaplastic cellular changes. Slight to moderate cellular alterations were found in 202 (31%) out of 656 cases. Metaplastic and inflammatory lesions were identified in 26 (35%) out of 74 endocervical biopsies. We found no increased incidence of dysplastic lesions in women wearing an intrauterine device. Inflammatory and metaplastic cellular alterations have been seen more frequently in women with IUD than in the controls. The duration of time the IUD were in situ, did not have an effect on the cellular alterations. "Actinomyces-like" organisms were described in 38 (6%) out of 656 cases. The cytopathologist may be able to identify an "IUD-related" specimen in about one third of PAP-smears from women with an IUD, when he is aware that cervical smears from women having IUD may present with cellular alterations.

Cervix Uteri↗
PubMed1985

Connective tissue changes associated with inferior epiblepharon, epicanthus, and keratoconus.

A 34-year-old woman with bilateral inferior epiblepharon, epicanthus, and keratoconus is presented. Biopsies of right upper arm skin, left lower eyelid, and left cornea were sectioned and examined by electron and indirect immunofluorescent microscopy. Pathologic changes in both the skin and cornea were observed. Indirect immunofluorescent techniques showed an increase in fibronectin and procollagen type I in the subcutaneous tissue of the skin and an increase of procollagen type I in the deep dermis of the right upper arm skin and left lower eyelid. Increased amounts of laminin in the basal epithelium of the cornea and of collagen type III in the stroma and subepithelial components of the stroma were observed. Electron microscopy disclosed disordered arrays of collagen fibers in the right upper arm skin and left lower eyelid, and typical changes of keratoconus, with scars in the cornea. Both the cornea and skin had thin fibrillar extensions between collagen fibers.

Adult↗
PubMed1985

Correlation of hormone levels and quantitative histological findings in testicular biopsies.

Testicular biopsies of 84 oligo- or azoospermic males with gonades of nearly normal size and without chromosomal abnormalities were studied by morphometric methods. The mean Johnsen score and the results of the morphometric investigations were plotted against the results of the hormone assays (plasma testosterone, LH, FSH, prolactin). High LH and FSH levels showed a very good correlation with the loss of germ cells, the tubular shrinkage and interstitial fibrosis. Plasma testosterone levels, however, did not correlate with any morphological structure of the testicular parenchyma. The Johnsen score and the morphometric findings can to some degree explain the relationship between morphological abnormalities and endocrinological findings.

Biopsy↗
PubMed1985

Human chorionic gonadotropin-positive seminoma: is this a special type of seminoma with a poor prognosis?

Prognosis and treatment of HCG-positive seminomas with syncytiotrophoblastic giant cells are still a matter of discussion. Between 1977 and 1982 the serum HCG and alpha-feto protein levels were determined in a prospective study of a total of 51 patients presenting with histologically proven pure seminomas. A total of 13 of the 51 patients with pure seminoma had elevated serum HCG levels, but all of them were negative for alpha-feto protein. On clinical examination six patients had tumor Stage I, six had had tuor Stage II A-B and one patient had a tumor Stage III. The clinical stage was not found to correlate with HCG levels. In six patients presenting with tumors of clinical Stage II who underwent orchiectomy and subsequently radiation therapy there was a decrease in HCG levels following therapy. Five patients were followed for five years after therapy had been discontinued. There is no indication of a relapse in four patients, and the patients are negative or HCG. One patient suffering from a tumor, Stage IIB died three years later as a result of cirhosis of the liver. Autopsy did not reveal any signs of a residual tumor; the serum HCG levels were negative. For the time being HCG-positive seminomas should be classified and treated as a special morphologic form of seminoma.

Adult↗
PubMed1985

Testicular torsion: simple grading for histological evaluation of tissue damage.

Histological investigation of 63 biopsies taken during surgical detorsion of twisted testes reveals 3 morphologically different degrees of damage. In grade 1 injury the testicular parenchyma shows edema of interstice, slight blood extravasation and a desquamation of the germ cells. The grade 2 injury is characterized by a diffuse hemorrhagic saturation of the interstice and partial necrosis of germ cells. The grade 3 injury corresponds to a fully developed hemorrhagic infarction of the testis. The biopsies taken within the first 4 h after onset of symptoms show a relatively high number of minor changes (grade 1) which hold out hope for restitutio ad integrum. Grade 2 injury predominates in the period between 4 and 8 h, but it can appear also in the first 4 h. Only after 24 h all the testes examined are completely necrotic. This study shows that the amount of time that elapses between onset of symptoms and surgical detorsion is not the only factor to predict the outcome of the injured testis.

Adolescent↗
PubMed1985

Extraction of cells from paraffin-embedded tissue sections for single-cell DNA cytophotometry.

A simple technique is presented for the isolation of cells from paraffin-embedded tissues for Feulgen DNA cytophotometric investigations. Tissue fragments from paraffin blocks were deparaffinized in xylene, rehydrated and refixed in a formalin solution and incubated in a solution of 0.5 pepsin in 0.25% hydrochloric acid. After filtration through a 70 micron mesh and centrifugation, the cells were smeared upon a glass slide. Comparison between the results obtained with freshly prepared imprints and with pepsin-extracted cells of the same tumor showed that the extraction technique does not influence the Feulgen reaction or the DNA distribution pattern. Investigations carried out on bladder and embryonal carcinomas have demonstrated that the method permits an analysis of histologically or histochemically identified tumor cells within individual tissue areas.

Cell Separation↗
PubMed1984

DNA-Feulgen-cytophotometric analysis of single cells isolated from paraffin embedded tissue.

This study describes a method for the isolation of cells from paraffin embedded tissues for DNA-Feulgen-cytophotometric measurements. The relevance of the method used is demonstrated by the analysis of the DNA-distribution pattern of cells isolated from an alveolar sarcoma of the soft tissues of a 10-year old girl. The comparison between freshly prepared imprint preparations and specimens after pepsin--extraction clearly shows, that the preparation mode used did not influence the relative DNA-content. The mean values of DNA/nuclei did not show a decrease even after prolonged pepsin treatment (90 minutes). Therefore this method permits access to stored histopathological material for retrospective DNA-Feulgen-Cytophotometric investigations.

Child↗
PubMed1984

[Comparative histopathologic and sonomorphological prostate studies].

Ten cadaver prostates were divided into 50 sections and corresponding sections were compared, using ultrasound, with respect to their echogenic structure and histomorphology. The results of normal prostatic tissues, benign prostatic hyperplasia and prostatic carcinomas are described and discussed. It was shown that malignant prostatic lesions showed echo-poor sonograms in vitro. This observation is supported by our clinical experience. Possible explanations are discussed.

Adenocarcinoma↗
PubMed1984

Biological behaviour and DNA cytophotometry of urothelial bladder carcinoma.

In this prospective study, primary urothelial bladder tumour specimens from 64 patients were investigated by means of DNA Feulgen cytophotometry. The patients were followed clinically for at least 7 years. The objective cytophotometric parameters correlated well with the histopathological grading and the cytochemical data were of considerable prognostic value. They were closely correlated with the stage of the tumour, 5- and 7-year survival rates and the probability of tumour recurrence. It is concluded that DNA cytophotometry can serve as a method for the standardisation of histological grading and can give more objective and more detailed prognostic information than conventional histopathology.

Adult↗
PubMed1984

Morphology and incidence of testicular tumors in western Austria (1945-1980).

During the years 1945-1980 260 testicular tumors were registered in the two western provinces of Austria ( Tyrol and Vorarlberg ). The histological slices of these tumors were revised and classified according to the revised "British Classification" ( Pugh 1976). 91.9% of investigated testicular tumors were of germinal cell origin: 43.8% of them were seminomas and 38.1% teratomas. Combined tumors first appeared regularly when the surgical material was completely processed. The right side was more common affected than the left, in the ratio 7:5. The age of orchidectomy showed the typical peaks for seminomas, teratomas and combined tumors. 60% of germinal cell tumors were operated in stage pT3 (UICC). The average incidence of all testicular tumors in the years 1945-1980 was 1.9/100,000 males, but in the last decennium (1971-1980) the average incidence was 4.0, with a maximum of 6.9 in 1978. Districts with a high percentage of employed in agriculture and forestry showed lower incidences than industrialized areas.

Adolescent↗
PubMed1983

[Ultrasonically guided fine-needle biopsy].

Ultrasonically guided fine-needle biopsy with cytologic evaluation (UGF) was performed in 347 patients with suspected tumors mostly of the abdomen. With an accuracy rate of more than 90% UGF proved to be a safe investigation of high diagnostic value.

Abdominal Neoplasms↗