Search PubMed⌕ Search

Biomedical subjects

G Li

Publications and source records attributed to G Li.

At least 559 records · Page 31Linked to original sources
PubMed1998

[Sjögren's syndrome and malignant lymphoma].

To diagnose and study the early stage of Sjögren's syndrome associated with malignant lymphoma, the authors used the PCR technique of B malignant lymphoma gene for lacrimal gland tissues in four patients with Sjögren's syndrome and in five patients suffering from B malignant lymphoma. The results showed that all cases of Sjögren's syndrome were negative and all cases of B malignant lymphoma were positive. These suggest that in general the patients with primary or secondary Sjögren's syndrome have no malignant lymphoma genes, but those who have chronic fever and enlarged superficial lymph nodes should under go this technique for early diagnosis and treatment of Sjögren's syndrome associated with malignant lymphoma.

Adult↗
PubMed1998

Changes of peripheral tissue thyroid hormone metabolism in rats fed with selenium- and vitamin E-deficient artificial semisynthetic diet.

OBJECTIVE: To examine the relationship between selenium (Se)- and vitamin E(VE)-deficiency and thyroid hormone (TH) metabolic disturbance, especially type I iodothyronine 5'-deiodinase (ID-I) activity. METHODS: We observed the metabolic changes of TH-3, 3',5-triidothyronine (T3) and thyroxin (T4) and their free radicals-glutathione peroxidase (GSH-Px) and lipid peroxides (LPO) in the peripheral tissues (liver, kidney and blood) of Wistar rats maintained on Se- and VE-deficient artificial semi-synthetic diet for 8 weeks. RESULTS: In the Se- and VE-deficient rats (compared with Se- and VE-supplement rats): 1). Hepatic and renal ID-I activities decreased by 60% and 50% respectively, serum levels of T3 reduced 36%, and T4 increased by 32%. Hepatic ID-I activity and serum T3 concentration in Se- and VE-supplement rats were significantly higher than those in the Se-supplement and VE-deficient rats. 2). GSH-Px activities in the whole blood and liver decreased by 61% and 82% respectively. LPO concentrations in serum and liver increased by 53% and 40% respectively. When Se content remained the same, changes of VE content did not significantly affect GSH-Px activity, but the combined supplement of Se and VE decreased significantly the LPO concentration. CONCLUSIONS: The data suggests that GSH-Px activity is influenced mainly by Se level, but ID-I activity is influenced by VE concentration in addition to Se level. VE seemed to play a protective role on ID-I and its mechanism might be related to the fact that VE protects the stability of microsomal membrane in which ID-I exists, avoiding from free radical damage as well as the coordinated action and mutual sparing effect of Se and VE.

Animals↗
PubMed1998

[Magnetic resonance imaging of hypopharyngeal and cervical esophageal cancer].

Imaging by MR (MRI) and esophageal barium meal of 17 cases of hypopharyngeal and cervical esophageal cancer were analyzed. The results indicate that esophageal barium meal can not demonstrate the invading of neoplasms to the adjacent structure. Therefore, it is difficult to use the only data of esophageal barium meal for TNM staging. The results also indicate that MRI is more useful and reliable in assessing the three dimensional structure and the neoplasms invading to the adjacent structure. This results suggest that MRI is that useful in determining the diagnosis, TNM staging and the determining the appropriate operative therapy for hypopharyngeal and cervical esophageal cancer.

Adult↗
PubMed1998

Ischemic preconditioning enhances donor lung preservation in canine lung transplantation.

OBJECTIVE: To prove the protective effect of lung ischemic preconditioning on enhancing the canine lung preservation and reducing allograft lung dysfunction after transplantation. METHODS: Ten pairs of adult canines underwent left lung allotransplantation. Five donors were treated with ischemic preconditioning [their left hilus was clamped for 10 minutes and released for 15 minutes (Group IP)], and five donors were not treated with ischemic preconditioning (Group C). The donor lungs were flushed with 4 degrees C Euro-Collin's solution (ECS) and stored in the same solution for two and a half hour, then transplanted to the recipient canines, who were observed for one to two hours after transplantation. The lung venous blood of the recipient and the donor lung tissue were collected just after thoracotomy and one hour after reperfusion of the transplanted lung in both groups. RESULTS: The number of polymorphonuclear (PMN) was significantly higher in Group IP than in Group C (P < 0.05). However, the number of PMN in lung interstitium under microscope was less in Group IP than in Group C. The thromboxane (TXB2), malondialdehyde (MDA) and mean pulmonary artery pressure (MPAP) contents were significantly lower in Group IP than in Group C (P < 0.05). The superoxide dismutase (SOD) and the lung venous blood oxygen tension (PvO2) contents were significantly higher in Group IP than in Group C (P < 0.05). Histological findings showed less damages in Group IP than in Group C. CONCLUSIONS: The protective effect of ischemic preconditioning together with ECS flush and storage is superior to using ECS alone. The possible mechanisms may be that ischemic preconditioning inhibits the accumulation and activation of PMN in lung tissue and reduces the production of oxygen free radicals.

Animals↗
PubMed1998

[Function analysis of regulative region of transforming gene Tx related to human nasopharyngeal carcinoma].

Tx gene is the DNA sequence with transforming activity which was cloned from the epithelial cell line (CNE2) of human nasopharyngeal carcinoma (NPC). In order to understand the role of Tx in carcinogenesis of NPC, the promoter function of potential promoter region in Tx2. 8 was detected using CAT reporter assay. The results showed that the region has no promoter function. It suggested that Tx2. 8 kb fragment homogenous to IGKC might be regulated by the specific mechanism of immunoglobulin gene.

Chloramphenicol O-Acetyltransferase↗
PubMed1998

[Study on androgen receptor mRNA expression on laryngocarcinoma tissue].

Exploring the role of androgen receptor (AR) in development of laryngocarcinoma, expression of AR in larynx squamous cell carcinoma and normal larynx tissue were observed and analysed by Northern blot hybridization. The results showed: AR mRNA was founded in both 27 larynx squamous carcinoma and 6 normal larynx tissue beside carcinoma samples. The intensity of AR mRNA expression in carcinoma was significantly stronger than that in normal larynx tissue, and the expression of AR mRNA was in low degree in normal larynx tissue. The existence and different expression of AR mRNA in both laryngocarcinoma and normal larynx tissue suggest that AR gene can control development of laryngocarcinoma tissue.

Carcinoma, Squamous Cell↗
PubMed1998

[Genetic instability of microsatellites Mfd41 and DCC in the evolution of chronic myelogenous leukemia].

OBJECTIVE: To explore the relationship between the genetic instability of microsatellite and the evolution of chronic myelogenous leukemia(CML). METHODS: The loss of heterozygosity(LOH) and the microsatellite instability(MSI) of two polymorphic microsatellite markers, DCC and Mfd41, which located on chromosome 18q and 17p respectively, were assayed by standard PCR-silver staining analysis in bone marrow cells from 17 CML patients progressing from chronic phase to accelerated phase or blast crisis. RESULTS: LOH or MSI of the two microsatellites were demonstrated in 8/17 (47.5%) patients in accelerated/blastic phases. For DCC, genetic instability was revealed in 2 of 9 patients in accelerated phase and in 4 of 8 in blast crisis. For Mfd41, genetic instability was revealed in 1 of 9 patients in accelerated and in 1 of 8 in blast crisis. CONCLUSION: Genetic instability of DCC and Mfd41 may play a role in the evolution of CML.

Adolescent↗
PubMed1998

[Observation on inhibitory effect of tetrandrine on the growth of human skin fibroblast in vitro].

The effect of tetrandrine on the growth of human skin fibroblasts was observed in vitro culture. The result revealed that tetrandrine significantly inhibited the growth of fibroblast. The higher the concentration of tetrandrine, the higher the inhibition rate, forming a time-dependent relationship. The possible value of tetrandrine in the treatment of hypertrophic scar is discussed.

Alkaloids↗
PubMed1998

[Hodgkin's disease: Ig gene rearrangements in single H/R-s cells].

OBJECTIVE: To investigate the origin and cell lineage of Hodgkin/Reed-Sternberg (H/R-S) cells from various subtypes of Hodgkin's disease (HD). METHODS: 68 single H/R-S cells were taken from frozen section of 8 cases of HD. The DNA from these single cells was amplified by polymerase chain reaction (PCR) with immunoglobulin heavy chain gene FR III a/JH primers and light chain family-specific primers. RESULTS: (1) IgH and V kappa 4 rearrangements were repeatedly found in H/R-S cells from one case of lymphocytic predominant HD (LPHD). (2) Repeated and unique IgH and V kappa 2,4 rearrangements were observed in 1 case of nodular sclerosing HD (NSHD) and unique IgH, V lambda 3/V kappa 4 rearrangements in another case of NSHD. (3) Repeated IgH/V lambda 3 and unique V lambda 2,4 rearrangements, repeated V kappa 4 and unique IgH/V kappa 3 rearrangements, plus repeated IgH and unique V kappa 3/V lambda 4 rearrangements were detected respectively in 3 cases of mixed cellularity HD (MCHD). Repeated and unique rearrangements were found respectively in 2 cases of MCHD. CONCLUSIONS: The H/R-S cells isolated from LPHD had monoclonal Igh and V kappa 4 gene rearrangements, which supports the conclusion that LPHD is a monoclonal proliferation of B cells. The appearance of IgH and kappa, lambda gene rearrangement suggest that the H/R-S cells isolated from classical HD (MCHD and NSHD) originate from B lineage cells at various stages of differentiation.

Gene Rearrangement, B-Lymphocyte, Light Chain↗
PubMed1998

[Changes of nitric oxide synthase in hypoxic pulmonary hypertension and the effect of L-arg and L-NAME on pulmonary circulation].

OBJECTIVE: To study the role of nitric oxide (NO) in Hypoxic pulmonary hypertension (HPH). METHODS: NADPH-d and immunohistochemistry were used to study the expression and localization of constructive and inducible nitric oxide synthase (cNOS iNOS) in the lungs of normoxia and HPH rats, at the same time, the effects of L-arginine (L-arg) and nitro-L-arginine-methyl-ester (L-NAME) on normoxic and hypoxic pulmonary circulation were investigated. RESULTS: NADPH-d positive expression was detected in the endothelium and smooth muscle cells (SMC) of large pulmonary vessels (PV) and in the epithelium and SMC of bronchi in the HPH group, but negative in the endothelium and SMC of small PV in normoxia. Hypoxia not only inhibited the expression of cNOS in the endothelium and SMC of PV and epithelium of bronchi, but also induced the expression of iNOS in the endothelium and SMC of PV and SMC of bronchi. Administration of L-NAME in normoxia had no effect on pulmonary circulation. The effect of administration of L-arg and L-NAME in hypoxia on right ventricular hypertrophy and remodeling of PV had no significance, compared with simple hypoxia. CONCLUSION: The inhibition of cNOS may contribute to the occurrence of HPH, but induced iNOS may depress the development of HPH.

Animals↗
PubMed1998

[The preparation of chiral column of cellulose triacetate coated on small pore silica gel and the separation of enantiomers].

The cellulose triacetate (CTA) prepared by heterogeneous acetylation and coated on small pore 3-aminopropyl silica gel (10 nm) was used as chiral stationary phase for HPLC (OA column). The racemes of three drugs, two fulvenes and one dazine, were separated on this column. Among these racemes, timolol maleas was separated using 1.0 mol/L NaClO4:95% ethanol = 10:90 as mobile phase. Without NaClO4 in mobile phase, the resolution of timolol maleas can not occur. Ethanols of 95%-98% were used as mobile phase for other racemes. The influence of the content of water in mobile phase on the chiral separation of praziquentelum, the retention time and separation factor (alpha) were reduced with the increase of water content in mobile phase. When the content of water in the mobile phase was 13%, praziquentelum could not be separated. It was found that in the case of samples No. 1 and No. 2, when the substituents R1 and R2 or R'1 and R'2 of fulvenes are 4-methoxybenzoyl and 4-methoxyphenyl, the chiral separations of fulvenes can be obtained. For sample No. 4 when R1 and R2 of fulvenes are 2-methylbenzoyl and 2-methylphenyl or in the case of No. 5, when R1 and R2 are 5-bromo-2-furoyl and 5-bromo-2-furan, the chiral separations of fulvenes can not be achieved. For sample No. 6 and No. 7, when R'1 are 4-chlorobenzoyl and benzoyl or R'2 are 4-chlorophenyl and phenyl, the chiral separation also can not be achieved. The column performance and separation factor were not obviously reduced after used for four months.

Cellulose↗
PubMed1998

[Determination of antioxidants BHT,BHA and PG in food with high performance liquid chromatography].

An accurate and rapid method for simultaneous determination of antioxidants butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and propyl gallate (PG) in food by reversed-phase high performance liquid chromatography was established. The sample was extracted with hexane. Being filtrated and dried by N2, the residues in evaporator was dissolved in a certain amount of water-C2H5OH (1:4, V/V) and filtered with 0.5 micron of filter membrane for HPLC analysis. The chromatographic conditions were Radial-PAK C12 column, methanol-water (92:8, V/V) mobile phase adjusted to pH = 3 with phosphoric acid and UV-280 nm detector. By using external standard method the analytical results showed that the coefficients of variation of PG,BHA and BHT were 0.61, 0.08 and 1.44 respectively, linear correlation coefficients were more than 0.999 and recoveries were 92%-98% (n = 6). The lowest detection limit was 0.5 mg/L.

Antioxidants↗
PubMed1998

[Effect of Jianpi Yiqi recipe on the heat shock proteins in rats with acute injury of gastric mucosa].

OBJECTIVE: To investigate the mechanism of Jianpi Yiqi Recipe (JPYQR) in inhibition of gastric mucosa injury. METHODS: A rat model of acute injury of gastric mucosa was established by binding and immersing in water. Ulcer index (UI) was measured, change of heat shock protein (HSP70) in plasma and gastric tissue was examined, and the gastric HSPs was studied immunohistochemically. RESULTS: The UI in rats pretreated with JPYQR or Lizhudele was lower than that of model group (P < 0.001), and UI of JPYQR group was lower than that of Lizhudele group (P < 0.01). On the other hand, the HSP70 level in the two pretreated groups were significantly higher than that of the model group (P < 0.05), and the plasma HSP70 level of JPYQR group was higher than that of Lizhudele group (P < 0.05). The difference of gastric HSP70 level between the two pretreated groups was insignificant (P > 0.05). Immunohistochemical study revealed a high level expression of HSP70 and HSP60 by glands and the level of the expression in model group was the highest, followed by those in Lizhudele group and JPYQR group. CONCLUSION: JPYQR could enhance the expression of HSPs in rats, and so as to prevent the gastric mucosa from injury. HSPs might involve in the defending mechanism of gastric mucosa.

Animals↗
PubMed1998

[Effect of garlicin on unstable angina pectoris and its relationship with blood lipid and GMP-140].

OBJECTIVE: To observe the clinical effect of Garlicin on unstable angina pectoris (UAP) and to explore the relationship between Garlicin's effect and Syndrome Differentiation of TCM. METHODS: Fifty-five patients of UAP were divided randomly into the Garllcin group (34 cases) and the control group (21 cases) and classified based on Syndrome Differentiation of TCM into Cold Syndrome type, Heat Syndrome type, severe blood stasis (SBS) type or mild blood stasis (MBS) type. Garlicin 60 mg or nitroglycerin 5 mg were given to the two groups respectively by intravenous drip for 10 days as one therapeutic course. The curatic effect was evaluated by changes of symptoms and electrocardiogram, and some indexes, such as blood lipid, lipoprotein, apolipoprotein and GMP-140 among different groups and types were compared. RESULTS: The difference of effective rate between the Garlicin group and the control group was insignificant. In the Garlicin group, the effective rate of Cold Syndrome type was higher than that of Heat Syndrome type (P < 0.01), and HDL/LDL ratio and apoA1 level raised markedly in the former type (P < 0.05) while an oppositive trend revealed in the Heat Syndrome type. Garlicin showed a higher effect on SBS type than that on MBS type and cause GMP-140 of the MBS type decreased obviously. CONCLUSION: Garlicin is effective on UAP patients and its effect was more evident on those with Cold Syndrome type or SBS type. Its mechanism may be related to its effect in improving blood lipid and enhancing activation of platelet.

Aged↗
PubMed1998

[Clinical study on effects of zhiling decoction in treating senile vascular dementia].

OBJECTIVE: To observe clinical effects of Zhiling Decoction (ZLD) in treating senile vascular dementia. METHODS: Seventy-eight patients were randomly divided into treated group and control group. In treated group which was treated with ZLD, a self-formulated proved recipe. In control group, which was administered with Naofukang ([symbol: see text]). RESULTS: ZLD could decrease the level of serum cholesterol, serum triglyceride and plasma LPO significantly; increase the level of high density lipoprotein (HDL) and SOD in red blood cells; dilate cerebral vessels, increase cerebral blood flow. As for P300, an index to reflect intelligence, the latency of P3 was shorter and the amplitude was raised, the brain electrical activity monitoring (BEAM) was improved remarkably, the revised Nagayakawa dementia scale scoring (NDS) of patients promoted, clinical symptom was improved significantly. Total effective rate in clinical practice reached 87%. CONCLUSION: ZLD had good effects on senile vascular dementia indeed.

Aged↗
PubMed1998

[Effect of kidney yin-yang replenishing Chinese herbs on the binding character of insulin receptor on hepatocyte membrane in androgen-induced sterility rats].

OBJECTIVE: To observe the effect of Kidney Yin-Yang replenishing Chinese herbs on the binding character of insulin receptor (IR) on hepatocyte membrane from androgen-induced sterility rats (ASR). METHODS: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. RESULTS: There was no significant difference of the number or affinity for both high and low affinity sites of IR between normal and ASR. CONCLUSION: The treatment with Kidney Yin-Yang replenishing Chinese herbs had no effect on insulin binding although a relatively high rate of ovulation induction on ASR was observed.

Animals↗
PubMed1998

[Blocking action of tetrandrine on the scar-collagen matrix contraction stimulated by transforming growth factor beta].

OBJECTIVE: To investigate the effect of Tetrandrine on the scar-collagen matrix contraction stimulated by transforming growth factor beta (TGF-beta). METHODS: The scar-derived fibroblasts were suspended in a solution of collagen matrix, and TGF-beta, they were associated to form a scar model in vitro. The influence of tetrandrine on the contraction indexes was observed. RESULTS: Tetrandrine plays a blocking action in the scar-collagen matrix contraction stimulated by TGF-beta, which was in a dose-dependent manner. CONCLUSION: This function of tetrandrine could be responsible for the anti-scar mechanism.

Alkaloids↗