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Biomedical subjects

G Leone

Publications and source records attributed to G Leone.

At least 379 records · Page 21Linked to original sources
PubMed1988

Management of pregnancy in women with antithrombin III congenital defect: report of four cases.

Four pregnant women with antithrombin III congenital deficiency underwent thrombosis prophylaxis including oral anticoagulants administered from the 16-18th week to the 36-37th week of pregnancy, subcutaneous heparin before the 16-18th week and after the 36-37th week, and a single infusion of AT III concentrate in the peripartum period in order to obtain a minimal level of 0.8 U/ml of AT III functional activity. The level of circulating AT III after the concentrate infusion needs to be evaluated by functional methods, because of a consistent amount in the concentrates of inactive AT III immunoreactive material. No thrombotic or haemorrhagic complication occurred after starting prophylaxis in any woman either in any newborn.

Administration, Oral↗
PubMed1988

Stepwise multivariate discriminant analysis (SMDA) for paired and unpaired biomedical data using microcomputers.

It may be necessary in biostatistics to discriminate between individuals and groups. The discriminant analysis is used for this purpose. The discriminant procedure has been programmed for microcomputers. The program has been written in a generic BASIC in order to make the procedure user-friendly. To have such a software package implemented on a personal computer may greatly facilitate the use of this complex analysis in biomedicine.

Computers↗
PubMed1988

Factor X Roma: a congenital factor X variant defective at different degrees in the intrinsic and the extrinsic activation.

A factor X molecular variant was identified in a 13-year-old girl affected by a bleeding tendency. Factor X antigen levels and activation by Russel's viper venom (tested both by clotting and amidolytic assays) were normal. Factor X crossed immunoelectrophoresis was found to be identical to that of the control plasma. Factor X functional activity was low (3% of the normal) if tested by PTT-derived assays, whereas it was found at intermediate levels (about 30-50% of the normal) if measured by prothrombin time-derived assays. The defect in the extrinsic activation was more clearly disclosed using as activating agent thromboplastin from ox brain. The factor X of the patient was completely adsorbed by aluminum hydroxide. The parents of the propositus (first degree cousins) showed factor X functional levels compatible with a condition of heterozygosity for the abnormality. This factor X molecular variant appears different from the other ones so far described and was named 'Factor X Roma'.

Adolescent↗
PubMed1988

Partial duplication of chromosome 1q preceding the development of an L3 lymphoblastic leukemia with t(8;14), secondary to treatment for Hodgkin's disease.

We report on a case of secondary lymphoblastic leukemia L3 in a patient treated with chemo- radiotherapy for a previous Hodgkin's disease. The typical chromosome translocation t(8;14) was found in association with a translocation t(9;19) and a marker chromosome 1, dup(1q). The same marker chromosome had been detected in a few cells of peripheral blood 2 years before the onset of the acute leukemia and may represent a proliferation-associated change, responsible for the initiation of the process of leukemogenesis.

Adult↗
PubMed1988

Plasma levels and loss of antithrombin III in chronic ambulatory peritoneal dialysis and nephrotic patients.

Plasma antithrombin III (AT III) levels were measured as antigen concentration (radial immunodiffusion) and as heparin cofactor (amidolytic method) in 9 patients on continuous ambulatory peritoneal dialysis (CAPD). The loss of albumin, proteins, AT III antigen and AT III functional activity was calculated from the peritoneal dialysate and the corresponding serum levels were measured. The same determinations were performed on serum and urinary samples from 9 patients with nephrotic syndrome. Mean plasma levels of AT III antigen and AT III heparin cofactor in CAPD patients were normal, whereas nephrotic patients showed a reduction in these values within a wide range. However, the loss of AT III antigen was similar in both groups and was strictly correlated to the loss of albumin. Most AT III in the peritoneal dialysate from CAPD patients was still active, whereas in nephrotic patients only 26% of the excreted AT III was functionally active. The difference in plasma AT III was functionally active. The difference in plasma AT III levels between these two groups, in spite of the roughly similar amounts recovered in the dialysate and in the urine, might be explained by an additional AT III loss in nephrotic patients due to renal metabolism.

Adult↗
PubMed1988

Multiple polymorphic sites in factor X locus.

The structure of factor X (FX) gene was analyzed in five FX deficient pedigrees with four different variants of the disease, as well as in 50 normal subjects. Genomic DNA from the deficient patients and the normal controls was digested with 12 restriction endonucleases and hybridized with a FX cDNA probe. The results seemingly exclude gross gene deletions or rearrangements in the deficient patients. A variety of polymorphic sites (ie, EcoRI, HindIII, PstI, PvuII, TaqI) was observed within the FX locus and their relative frequency was established. Intriguingly, a highly polymorphic region for the PvuII endonuclease was identified and located approximately 3 kilobases (kb) from the last 3' exon. These polymorphisms allowed us to analyze the allelic segregation in a FX deficient family and to identify a homozygous subject.

Adult↗
PubMed1988

[Ultrasonics of the liver (and bile ducts). The most significant experiences collected at a district hospital (clinical cases)].

The undoubted advantages deriving from the use of abdominal ultrasonography both in diagnosis and medical or surgical treatment are reported. In particular ultrasonography has proved very helpful in the study of pathologies of the liver and biliary tract, especially in a Rural Hospital, where it is not possible to have recourse to more refined diagnostic techniques.

Biliary Tract Diseases↗
PubMed1988

Increased platelet-fibrinogen affinity in patients with myeloproliferative disorders.

Patients with myeloproliferative disorders (MPD) are known to have some abnormalities of platelet glycoproteins (Gp). Quantitative changes of the Gp Ib, IIb-IIIa, and/or their glucidic content have been reported. Since the Gp IIb-IIIa complex plays a major role in fibrinogen binding by activated platelets, we measured the platelet fibrinogen affinity in nine patients with polycythemia vera (PV) and one subject with chronic myeloid leukemia (CML) by the aggregometric method of Marguerie. In all patients the Kd of the platelet fibrinogen reaction was significantly decreased as compared to controls, with evidence in two cases with PV of a heterogeneity of platelet-fibrinogen receptor sites. The measurement of 125I-labeled fibrinogen-platelet binding, performed in seven patients (five PV and two CML), showed receptor populations with increased (Kd1 = 0.58 + 0.3 X 10(7) mol/L) and normal affinity (Kd2 = 5.12 + 3.1 X 10(7) mol/L). These results demonstrate a heterogeneity of platelet-fibrinogen receptors in these patients and may explain the thrombotic diathesis of MPD subjects.

Adult↗
PubMed1988

Blood pressure monitoring from a biostatistical and clinical viewpoint for predicting fetal distress in preeclamptic pregnancy.

With technological progress, blood pressure monitoring has become a procedure largely applied in medical practice but not adequately systematized under a biostatistical viewpoint. This paper shows the complexity and importance of the biomathematical approach and its clinical implications in preeclamptic pregnancy. Data series were analyzed by means of noninferential and inferential procedures of biostatistics. A progressive gain of information is obtained, the inferential procedures showing an analytical resolution able to discriminate fetal distress in preeclamptic pregnancy.

Adult↗