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Biomedical subjects

G L Wantzin

Publications and source records attributed to G L Wantzin.

83 records · Page 5Linked to original sources
PubMed1977

Nuclear labelling of leukaemic blast cells with tritiated thymidine triphosphate in 35 patients with acut leukaemia.

A new technique which detects the presence of DNA polymerase and primer-template DNA by measuring the in vitro incorporation of [3H]thymidine-5-triphosphate (3H-TTP) into nuclei of leukaemic blast cells (LBC) was used in 35 patients with acute leukaemia. The 3H-TTP labelling index (3H-TTP LI) exceeded the fraction in DNA synthesis by a factor 1.4-24.3. The values of 3H-TTP labelling in the bone marrow always exceeded those obtained in the blood. In addition 10 normal bone marrows were studied; here, the 3H-TTP LI either exceeded or equalled the fraction of the proliferative pool in DNA synthesis.

Adolescent↗
PubMed1977

Trisomy 8 in acute myeloid leukaemia: A non-random event. Lack of correlation with prognosis and cytokinetic parameters.

6 cases of AML with a supernumerary chromosome 8 as the only aberration in practically all bone marrow mitoses and 10 cases with a normal chromosome composition of the marrow cells were investigated in order to evaluate the possible influence of trisomy 8 on some clinical and cytokinetic parameters. No significant differences between the groups were found. In our laboratory a supernumerary chromosome 8 is present in 36% of AML cases with chromosomal aberrations.

Adolescent↗
PubMed1976

Cell proliferation and protein synthesis in human leukaemic myeloblasts after cytosine arabinoside therapy.

The effect of a single intravenous bolus injection of cytosine arabinoside (ARA-C) on the cytokinetics and 3H-leucine uptake of leukaemic myeloblasts in bone marrow and blood was studied at intervals up to 96 h after ARA-C in five patients with previously untreated acute myelogenous leukaemia. An early decrease in mitotic index and in 3H-thymidine labelling was observed in four of five patients and pretreatment values were reached again within the observation period. Changes suggesting synchronization were not observed. ARA-C induced a marked decrease in protein synthesis in the leukaemic myeloblasts as estimated from either a decrease in the 3H-leucine labelling index or the mean grain count of 3H-leucine labelled cells. It is suggested that ARA-C is incorporated also by cell which are not in S-phase and has a prolonged biochemical effect on the leukaemic cells.

Adult↗
PubMed1976

Nuclear DNA-polymerase estimation in human leukaemic myeloblasts.

A technique which detects the presence of DNA-polymerase in cell nuclei by measuring the incorporation of 3H-thymidine-5-triphosphate (3H-TTP) has been used to estimate the proportion of leukaemic myeloblasts which contains DNA-polymerase and DNA capable of acting as primer-template. In six cases of previously untreated acute myeloid leukaemia the 3H-TTP labelling index (3H-TTP LI) was much larger than the fraction in DNA synthesis. After a single 'flash' injection of cytosine arabinoside a pronounced decline was observed in the 3H-TTP LI, which can be explained by a direct inhibition of NDA-polymerase. No change was observed in the fraction of cells labelled with 3H-thymidine. A decrease in 3H-TTP LI was also observed after a single i.v. dose of methotrexate.

Bone Marrow↗
PubMed1975

The NBT test: Erratic behaviour in acute leukaemia.

Non-stimulated and stimulated nitroblue tetrazolium (NBT) dye test was studied in sixteen adult patients with acute leukaemia in sequence, viz. at the time of diagnosis, during and after treatment. It was found that the capacity of the neutrophilic granulocytes to reduce NBT (non-stimulated) was below, within or above the range for the normal group. Two of the patients had a constant negative non-stimulated NBT test. The stimulated NBT test was below the normal range. Generally, there was no correlation between the percentage of NBT positive cells and fever of bacterial origin. All sixteen patients but four were found to be within the normal range concerning phagocytic ability.

Acute Disease↗
PubMed1975

Karyotypes in infectious mononucleosis.

Using a trypsin-Giemsa banding procedure, chromosome analysis was performed on blood from 21 consecutive patients hospitalized for infectious mononucleosis. Mitoses were harvested after 2 and 24 h in vitro incubation without PHA and after 48 h with PHA. No abnormalities were seen.

Adolescent↗
PubMed1975

The effect of prednisone on the non-stimulated and the stimulated NBT test.

The non-stimulated and stimulated nitroblue tetrazolium (NBT) dye test was studied in five normal adults in sequence, viz. before and at intervals after prednisone was given as one large dose. A negative effect of prednisone on the NBT response was seen. This applied to both the non-stimulated and stimulated NBT test. For the latter five different toxins were used. At the same time a pronounced neutrophil granulocytosis was seen. The depression of the NBT test is present both in the percentage of NBT-stained neutrophils and when expressed in absolute counts of NBT-strained neutrophils.

Adult↗