Biomedical subjects
Publications and source records attributed to G Kiss.
The authors report on the microsurgery method of the vascularized free fibula flap transplantation for the reconstruction of large segmental mandibular defect. They have established from the literatury data and by their own experience of two cases, that the use of free fibula flap seems to be the best replacement method, when the bone defect is bigger then twelve centimeters.
Antiphospholipid antibodies predispose to venous and arterial thrombosis. The case of a sixteen-year-old boy with primary antiphospholipid syndrome is presented. Features of different organ involvement such as thrombosis of the inferior vena cava, pulmonary thromboembolism and cerebral involvement were present with thrombocytopenia and high titre of anticardiolipin in the patient's sera. On twelve-month follow-up while taking anticoagulant the patient was entirely free of symptomes though certain immunological findings showed slight positivity. However, considering the ARA criteria systhemic immunopathologic disorder could not have been proved. Other pathological conditions with anticardiolipin positivity were also excluded.
Cyclins in association with the protein kinase p34cdc2 and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.
OBJECTIVE: We investigated the role of intravesical electrical stimulation in the treatment of voiding dysfunctions following major gynecologic surgery. METHODS: 19 female patients with sensory and/or motor voiding dysfunction following gynecologic operations underwent intravesical electrostimulation after failure of traditional treatments. Before and after therapy, urodynamic examinations were performed. The follow-up was 6-24 months. RESULTS: All cases of sensory bladder dysfunction were cured. Volumes of residual urine significantly decreased (mean 274 vs. 53 ml: p = 0.0003) and maximum detrusor pressure increased (mean 6 vs. 27 cm H20; p = 0.0007). An early start of therapy (within 6 weeks after surgery) resulted in a better outcome. CONCLUSIONS: Intravesical electrical stimulation was effective in the treatment of sensory and motor voiding dysfunctions following major gynecologic surgery.
Cytogenetical endpoints, i.e., chromosome aberration (CA), sister-chromatid exchange (SCE), and proliferative rate indexes (PRI), were measured in peripheral blood lymphocytes (PBL) of 42 workers exposed occupationally to low-dose benzene, and of 42 controls. The role of smoking habit as a confounding factor of genotoxic effects caused by occupational low-dose benzene exposure was also studied. The benzene concentrations in the ambient air samples varied from 3 to 20 mg/m3 (mean: 7 mg/m3). The continuous low-dose benzene exposure significantly increased the CA and SCE frequencies, but did not influence PRI. Smoking levels were characterized by subjective accounts and by serum thiocyanate concentrations (SCN). CA and SCE were not significantly increased in smokers compared to nonsmokers, but the differences were expressed to a greater extent in the case of measurement of SCN concentrations. Determination of SCN proved to be more objective in the assessment of genotoxic effects of smoking as a confounding factor of occupational low-dose benzene exposure.
Urinary delta-aminolaevulinic acid (delta-ALA) excretion was evaluated in random urine samples of 191 healthy children, aged 2-14 years, with blood lead levels < 0.8 mumol/l (mean +/- SD: 0.34 +/- 0.13), erythrocyte zinc-protoporphyrin < 70 mumol/mol haem (mean +/- SD: 50.4 +/- 8.0) and blood haemoglobin > 6.8 mmol/l (mean +/- SD: 8.2 +/- 0.5). It was found that uncorrected delta-ALA concentration and delta-ALA/creatinine ratio are age-dependent, whereas the ratio of delta-ALA/logarithm of creatinine concentration (mean +/- SD: 55.3 +/- 13.5 mumol/log mmol) is independent of age and sex. The authors recommend the use of this parameter for the assessment of delta-ALA excretion in random urine samples in children.
Thirty consecutive patients treated with a free radial forearm flap were reviewed. This flap was used in the reconstruction of intraoral defect in 24 patients and of extraoral defect in 6 patients. There were no total or partial flap failures. Donor site complication included a partial loss of skin graft in 4 and radial fracture occurred in 1 patient. The authors considered the application of the radial forearm flap a reliable method for resurfacing large skin defect of the face. However, according to their conviction the most important field of the forearm flap is its use in intraoral reconstruction after pull-through operation. They drew attention to the limitation of the use of osteocutaneous flap in the replacement of segmental mandibular defect.
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The authors present the case of a 47-year old female patient. She suffered from a moderate activity SLE for two years. The outcome of SLE was changed by an ulcer because of leg injury, which was repeatedly infected. Severe polyarthritis, leucopenia, thrombocytopenia, clinical signs of vasculitis and in the last two months fever, high ESR, and pericarditis appeared. The angina pectoris, the cardiac decompensation, the electrocardiogram, the echocardiogram was suspect to diffuse myocardial lesion. Cardiac decompensation caused the death of the patient. Besides the activation of her autoimmune disease, infection was suspect in the patient taken immunosuppressive and steroid drugs, though it could not be verified. Autopsy verified besides the recent necrosis of heart without reaction, and multifocal myocardial abscess, which appeared possible in the terminal phase.
We report a unique application of the Random Amplified Polymorphic DNA (RAPD) technique for genetic linkage mapping of a single spruce tree using haploid DNA from megagametophyte tissue of individual seeds. Sixty-one segregating loci were analysed for reproducibility, inheritance and linkage. Forty-seven of the 61 markers were distributed into 12 linkage groups and covered 873.8 cM. The 14 markers not associated with any linkage group should be assigned to linkage groups as more markers are added to the map. This new approach quickly provides molecular genetic markers that are simple to evaluate for constructing genetic linkage maps and for other related genetic studies in forest tree species.
Free fasciocutaneous forearm flaps, based on the radial artery, were used to replace the tissue defect caused by pull-through operations for cancer of the floor of the mouth in 11 patients. There was no total or partial flap failure. The greatest advantage of the method is that restriction of the tongue movement is less when compared with the results of other methods. The thin forearm flaps are adaptable and pliable. The newly developed lower alveolar crest and the sublingual sulcus make prosthetic rehabilitation possible.
The authors make known the indications and method of the free osteocutaneous forearm flap. They have established from the literature data, and by their own experience of 4 cases, that the use of free osteocutaneous forearm flap seems to be the most ideal method when the bone defect is not bigger than 6-8 cm and only the replacement of mucosa is required.
Free radial forearm flap was used to replace the tissue defect caused by pull-through operation for cancer of the mouth in 13 cases. There was no total or partial flap failure. The greatest advantage of the method is that the restriction of the tongue movement is less when compared to the results of other methods since the thin forearm flaps are well adaptable and are of a pliable nature. The newly developed lower alveolar crest makes the prosthetic rehabilitation possible.
The authors survey the possibilities of diagnosis of the frequent injuries of the hand caused by foreign bodies. It is proven by them that almost all foreign bodies can be demonstrated using radiological methods (native picture, ultrasound examination, xeroradiography, CT, MRI). They support the immediate surgical care in the therapy of injuries caused by foreign body, they deal however with the problems of the later therapy, too. Their opinion is backed up by the demonstration of case reports.
The work was aimed at finding whether the ratio of delta-aminolevulinic acid (ALA) and creatinine (Cn) concentration (ALA/Cn), presently used in occupational health practice for evaluation of lead exposure gives a better assessment of ALA excretion than uncorrected ALA concentration itself, as well as at finding a better, but not complicated method for adjustment. ALA and Cn concentrations were measured in untimed urine samples of altogether 390 men and women (age: 18-60 years) not occupationally exposed to lead. In agreement with others, ALA/Cn was found to be an unsuitable method of adjustment for the differences in ALA concentration due to the different concentrations of samples. This can be explained by the different renal handling of ALA and Cn, proved by the literature data. The exponential relationship between ALA/Cn and Cn concentration raised the possibility of adjustment to the logarithm of Cn concentration (ALA/log.Cn). This simple method provided a more reliable index, the value of which was independent of the actual Cn concentration of urines within a wide range (2-32 mmol/liter). The recommended biological limit value (70 mumol/log.Cn mmol) separates equally well from normal values, both in dilute and concentrated urines. The evaluation of occupational lead exposure might be more reliable using this index, instead of uncorrected ALA concentration or the ALA/Cn ratio.
STUDY OBJECTIVE: The aim was to investigate whether basal coronary vascular tone and myocardial perfusion depend upon endothelial nitric oxide (NO) synthesis. DESIGN: Myocardial blood flow and vascular resistance of the left and right ventricles were studied before and after intravenous infusions of either NG-nitro-L-arginine (L-NA), a specific inhibitor of NO synthase, or L-arginine, the precursor of NO synthesis. Radiolabelled microspheres were used to study myocardial blood flow in small tissue sections. EXPERIMENTAL MATERIAL: 14 anaesthetised male cats, weight 2.1-3.5 kg, were used. MEASUREMENTS AND MAIN RESULTS: Measurements were made before and 15 and 40 min after L-NA treatment (30 mg.kg-1 bolus followed by 1 mg.kg-1.min-1 infusion; n = 8), and before and 15 min after L-arginine treatment (30 mg.kg-1 bolus followed by 10 mg.kg-1.min-1 infusion; n = 6). L-NA significantly reduced coronary blood flow to the left and right ventricle, by 30(SEM 9) and 48(6)% respectively, after 15 min, but only to the right ventricle, by 45(8)%, after 40 min. Mean arterial pressure and myocardial vascular resistance were raised during the L-NA infusion. In contrast, L-arginine did not elicit any change in the variables studied. CONCLUSIONS: The conductance of the coronary vascular bed and the resting myocardial blood flow is regulated by L-arginine derived nitric oxide, and exogenous L-arginine availability is not a limiting factor in this NO generation.
The incidence of primary liver carcinoma in the last twelve years was investigated retrospectively in the county Vas. A continuously increasing tumor frequency was found, by the end of the period the value had doubled. The phenomenon attained nearly exclusively the male population while no change could be seen in females. For further clarification comparative investigations into the association between chronic HBV infection and chronic alcohol abuse as well and hepatic carcinoma were performed. Changes in these factors cannot fully explain the gross increase in hepatic tumor incidence, the effect of other risk factors has to be suspected.
A 52-year-old man developed isolated atrophy of the infraspinatus muscle. The suprascapular nerve was compressed at the spinoglenoid notch by a hypertrophied inferior transverse scapular ligament.