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Biomedical subjects

G Kavli

Publications and source records attributed to G Kavli.

At least 37 records · Page 2Linked to original sources
PubMed1984

High pressure liquid chromatographic separation of furocoumarins in Heracleum laciniatum.

Furocoumarins from chloroform extraction of Heracleum laciniatum were separated by high pressure liquid chromatography (HPLC) with microparticulate silica gel column investigating different concentrations of acetonitrile and water, acetonitrile and methanol and acetonitrile, methanol and water for mobile phase. With a mixture of 42:43:15 of the latter for mobile phase optimal separation was obtained of the 6 furocoumarins from the plant. Removal of lipid fractions of plant extractions with hexane is recommended to avoid damage to the HPLC column.

Chromatography, High Pressure Liquid↗
PubMed1983

Photochemical reactions of Heracleum laciniatum. Influence of dimethyl sulphoxide and corticosteroids.

Phytophotodermatitis of Heracleum laciniatum is quite common in Tromsø during the summer months. The clinical picture varies from mild to severe erythematous reactions with or without vesicles or bullae on the exposed parts of the body. Phototoxic reactions of Heracleum laciniatum are reported. The influences on phototoxic reactions by bergapten (5-methoxypsoralen) and UV-A by treating the skin with a membrane labilizing agent, dimethyl sulphoxide (DMSO), and a membrane stabilizing agent, the steroid desoximethasone, are evaluated.

5-Methoxypsoralen↗
PubMed1983

Phototoxic and contact toxic reactions of the exocarp of sweet oranges: a common cause of cheilitis?

Irritant skin reactions were produced within 1 h after application of the exocarp of sweet oranges or alcoholic extracts therefrom. Such reactions faded within 48 h. The exocarp, or extracts thereof, induced phototoxic reactions which were strongest at 72 h after exposure. The phototoxic reactions were only induced in natural blondes and only with some oranges. The in vivo phototoxic reactions were confirmed in vitro, causing a slight but clear photo-inhibition of Candida albicans. Only some oranges inhibited growth.

Cheilitis↗
PubMed1983

Furocoumarins of Heracleum laciniatum: isolation, phototoxicity, absorption and action spectra studies.

Isolation of the furocoumarins (psoralens) bergapten, isobergapten, sphondin, isopimpinellin and pimpinellin from the Umbilliferous plant Heracleum laciniatum was carried out by column chromatography, and the structure and absorption spectra for the 5 furocoumarins isolated are described. Photoepicutaneous testing showed the strongest phototoxic effects from bergapten, marked effects from pimpinellin, weak effects from sphondin and none from the others. These in vivo findings were confirmed by the in vitro Candida test. Action spectrum studies demonstrated peak photosensitivity in the range 330-335 nm, bergapten being more than twice as phototoxic as pimpinellin.

5-Methoxypsoralen↗
PubMed1983

In vivo and in vitro phototoxicity of different parts of Heracleum laciniatum.

Experimental phytophotodermatitis was produced using homogenates from different parts of Heracleum laciniatum and long-wave ultraviolet light. The homogenates of leaves and flowers produced strong phototoxic reactions, fruit slightly less. Stems were either non-phototoxic or only slightly phototoxic. The roots of young plants did not evoke photosensitivity reactions, whereas the roots of older plants were strongly phototoxic; even roots collected from the frozen ground in December elicited strong reactions. Abdominal and midback skin were more photosensitive than skin of the lateral aspects of the underarms and legs.

Candida albicans↗
PubMed1983

Phototoxicity from furocoumarins (psoralens) of Heracleum laciniatum in a patient with vitiligo. Action spectrum studies on bergapten, pimpinellin, angelicin and sphondin.

Investigations on light reactions in a patient with vitiligo are presented. The minimal erythema dose (MED) in the UVB area was approximately 1/3 of that in persons of skin type II. The application of furocoumarins (psoralens) increased light tolerance by 1 MED at 300-310 nm. Action spectrum studies with furocoumarins from Heracleum laciniatum showed the following order of potency: bergapten, pimpinellin, angelicin and sphondin. The efficacy was highest at 325-350 nm, with maxima at 330-335 nm. Pimpinellin was recently found to be phototoxic, but an action spectrum of sphondin is reported for the first time.

5-Methoxypsoralen↗
PubMed1983

Phototoxic dermatitis caused by celery infected by Sclerotinia sclerotiorum.

11 men developed a severe phototoxic dermatitis of the hands and forearms after harvesting celery infected with Sclerotinia sclerotiorum. By thin-layer chromatography and fluorescence spectrophotometry, xanthotoxin, bergapten and sphondin were isolated. The Canadida albicans test demonstrated xanthotoxin and bergapten as the phototoxic compounds.

Agricultural Workers' Diseases↗
PubMed1983

Phototoxicity of Heracleum laciniatum. Case reports and experimental studies.

Phytophotodermatitis from Heracleum laciniatum is described in 2 typical cases. Experimental phytophotodermatitis was produced using homogenates from leaves of the plant and long-wave ultraviolet light. The homogenates of leaves produced strong phototoxic reactions. The minimal phototoxic erythema dose was determined to be lowest for abdominal and midback skin. The action spectrum for Heracleum laciniatum homogenates applied topically was found to be in the range 315-375 nm with peak sensitivity at 330-335 nm.

Adolescent↗
PubMed1983

A case of chronic urticaria and vitiligo, associated with thyroiditis, treated with PUVA.

A case of chronic urticaria and vitiligo, associated with thyroiditis is described. Significantly elevated thyromicrosomal antibodies were found. Autoimmune reaction is a possible cause of the patient's thyroid disease, vitiligo and chronic urticaria. Following PUVA treatment with a total dose of 300 J/cm2 the patient's urticaria subsided in all irradiated areas, only to relapse 2 weeks later. Subsequently courses of PUVA gave periods of remission. The treatment also induced repigmentation in the vitiliginous spots. The results indicate a local PUVA effect upon mediators or mediator-containing cells.

Adult↗
PubMed1983

The pattern of lysosomal, cytosolic and plasma membrane enzymes in epidermis, dermis and suction blister fluid after ultraviolet radiation.

Slightly lower lysosomal enzyme activities were found in epidermis 18 h after irradiation with middle wave ultraviolet light while the values were invariably higher in the dermis of inflamed skin. The activities of the plasma membrane and the cytosol marker enzymes were higher in normal epidermis and dermis than in inflamed skin. Higher values were also found in suction blisters raised on inflamed skin, suggesting labilization/destruction of the plasma membrane during the inflammatory process.

Aged↗
PubMed1983

Mycosis fungoides in Norway 1960-80. A retrospective study.

The paper presents the results of a cooperative, retrospective study of mycosis fungoides in Norway, in 112 patients diagnosed histologically between 1960-80. The study includes an analysis of incidence, clinicopathological findings, survival, mortality and autopsy findings. In addition, other factors, such as occupation, the incidence of cancer, associated illnesses and contact allergies are reviewed. The significant prognostic variables with regard to survival included, age at diagnosis, stage of the disease at diagnosis and occupation. Industrial workers showed the highest incidence of MF and the poorest prognosis in terms of survival after biopsy. In 59% of deceased MF patients, the cause of death was related to MF.

Adult↗
PubMed1982

Accidental induction of photocontact allergy to Heracleum laciniatum.

Photocontact allergy to psoralens in Heracleum laciniatum occurred in two persons volunteering for investigations into phototoxicity of plant homogenates and purified psoralens. Photoallergy was noted following the fifth exposure in case 1, and the sixth in case 2. Testing with diluted solutions demonstrated allergy to sphondin, isobergapten and pimpinellin.

Accidents↗