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Biomedical subjects

G Isacsson

Publications and source records attributed to G Isacsson.

At least 91 records · Page 5Linked to original sources
PubMed1983

Intraoral salivary gland tumors: a retrospective study of 201 cases.

Two hundred and one cases of intraoral salivary gland tumors were studied from the files of the School of Pathology, University of the Witwaterstrand and South African Institute for Medical Research. 145 cases (72.5%) were classified as benign, comprising 140 pleomorphic adenomas and 5 monomorphic adenomas. The other 56 cases were classified as malignant or potentially malignant tumors, represented by 21 adenoid cystic carcinomas, 15 adenocarcinomas, 13 mucoepidermoid tumors, 5 carcinoma in pleomorphic adenomas and 2 epidermoid carcinomas. The patients in the malignant/potentially malignant group were significantly older than those in the benign group and a smaller proportion of their tumors were palatal, the difference being statistically significant. The high frequency of pleomorphic adenoma might result from a relatively higher number of black than white patients in this sample.

Adenocarcinoma↗
PubMed1983

An enzyme histochemical study of human salivary duct calculi.

An investigation was undertaken to study the distribution of enzymes associated with submandibular gland salivary calculi. Ten calculi were freeze-sectioned and incubated for acid and alkaline phosphatases and for lactate, succinate and maleate dehydrogenases. All calculi were partly covered by a 50-210 micrometers wide zone of organic material consisting of connective tissue and metaplastic squamous epithelium facing the mineralized calculus, or of a structureless substance attached to the mineralized calculus. The epithelium showed an intense staining reaction for acid phosphatase and lactate dehydrogenase and a moderate reaction for succinate dehydrogenase throughout all levels of the epithelium. The structureless peripheral zone exhibited a moderate activity of acid phosphatase and succinate dehydrogenase located to an area close to the mineralized matrix. Also alkaline phosphatase and lactate dehydrogenase were found in a special pattern in the structureless zone. Sodium fluoride and sodium vanadate added to the incubation medium inhibited acid phosphatase activity whereas cupric chloride only lowered the staining reaction. Enzyme activity was found only within the peripheral zone of organic material with one exception. The results suggest that the calcification process of salivary calculi is not a passive calcification of necrotic material or mucin but rather an active process promoted by enzymes in the surrounding organic substances.

Acid Phosphatase↗
PubMed1983

A combined clinical, mycological and histological study of denture stomatitis.

The clinical and histological appearance of the different types of denture stomatitis are in many respects very similar and confluent. In order to study the correlation between clinical and histological appearance and amount of yeasts, 48 patients with denture stomatitis and 24 with a healthy palatal mucosa were examined. The terms atrophic and hyperplastic denture stomatitis were introduced. The intensity of the palatal erythema and the growth of yeasts were most prominent among patients with hyperplastic lesions. Dryness and soreness of the mouth, angular cheilitis, spontaneous hemorrhage, glossitis and leukoplakias were found to a varying extent. No statistical evidence of correlation between the intensity of clinical erythema, amount of yeasts and grade of subepithelial inflammation could be found. The disease has most certainly a background other than solely increased growth of yeasts.

Adult↗
PubMed1982

The gigantiform salivary calculus.

The size of salivary calculi may range from small particles to large concrements of several centimetres. One case of a gigantiform salivary calculus located in the Wharton duct of a 48-year-old man is presented. After surgical removal of the 3.6 cm long concrement, it was split into 4 parts. The different parts were used for routine histological studies, production of microradiographs of ground sections and for scanning electron microscopy. Decalcified sections disclosed a specimen made of a homogeneous central nucleus and a peripheral lamellation. The same morphological picture was seen on the microradiographs, where the nucleus showed dense mineralization, and the peripheral part alternating rings of high and low mineral content. Microorganisms, mostly thread-like organisms, were found throughout the entire calculus in specially stained sections. Scanning electron microscopy confirmed the histologic and microradiographic findings. The presence of microorganisms also in the nucleus of the calculus seems to be one important aetiological factor favouring the formation of this gigantiform concrement.

Humans↗
PubMed1982

Salivary calculi as an aetiological factor in chronic sialadenitis of the submandibular gland.

During 1960-1975, 115 submandibular glands were removed for reasons other than neoplasia at the Ear, Nose and Throat department, Karolinska sjukhuset, Stockholm. The material was retrospectively analysed with regard to clinical and histopathological findings. The patients, 62 men and 52 women aged 13-77 years, exhibited in all cases but one a chronic sialadenitis. Salivary calculi were found in 82% of the patients. In one patient a mucous retention cyst was found. Microscopically some cases exhibited only a slight periductal inflammation, while others showed a complete destruction of the gland architecture. The duration of symptoms of the whole group before surgery varied from 1 week to 55 years but with a median value of almost 1 year. The conclusion of this study is that salivary calculus is the most important indication for removal of submandibular gland, for reasons other than neoplasia.

Adolescent↗
PubMed1981

Chronic sialadenitis of the submandibular gland. A retrospective study of 108 case.

The main etiologic factor in chronic sialadenitis of the submandibular gland is the salivary calculus. One hundred eight extirpated submandibular glands with the clinical diagnosis of chronic sialadenitis were histologically examined. The intensity of parenchymal destruction was related to the duration of symptoms prior to surgery. With the exception of four cases the glands showed histologically a varying degree of pathologic alterations. In almost one-third of the cases no acinar structures were found. The duration of symptoms ranged from 1 week to 55 years. No statistical evidence of correlation existed between the duration of symptoms and the degree of histological changes.

Adult↗
PubMed1981

Content and distribution of glycogen in oral epithelial dysplasia.

Forty-four oral lesions with epithelial dysplasia and 25 other benign and malignant lesions of the oral mucosa were examined after staining with hematoxylin-eosin and diastase controlled PAS. The intensity of the PAS-positivity for glycogen, the grade of dysplasia, the type of keratinization and the degree of subepithelial inflammation were recorded. Histologically normal epithelium at the margins of the lesions were used as controls. The presence and amount of glycogen in normal epithelium at the margins of the lesions were used as controls. The presence and amount of glycogen in normal epithelium at the margin of the lesions were used as controls. The presence and amount of glycogen in normal epithelium varied with the form of keratinization in that non- or parakeratinized epithelium was rich in glycogen whereas there was a negative glycogen-reaction in orthokeratinized epithelium. The most striking feature was an abrupt limitation of the glycogen at the junction between nondysplastic and dysplastic epithelium. The difference in the amount of glycogen in normal and dysplastic epithelium as assessed semiquantitatively, was statistically significant. There was no statistically significant difference in glycogen content with different degrees of dysplasia. The diastase controlled PAS-staining may therefore be a useful method of distinguishing dysplastic from nondysplastic epithelium in doubtful cases. Pseudoepitheliomatous hyperplastic epithelium covering granular cell myoblastoma did not contain any glycogen. Five of six squamous cell carcinomas and four verrucous carcinomas contained no demonstrable glycogen. Glycogen was present in the epithelium of the cases of lichen planus and "denture hyperplasia" investigated.

Adult↗
PubMed1980

Recurrent central odontogenic fibroma.

This article presents a case of a central odontogenic fibroma which recurred 9 years after it was enucleated. Very few cases of this tumor have been recognized and reported. The clinical and histopathologic criteria and the differential diagnosis of the lesion are stressed.

Adult↗
PubMed1980

Surgery in the treatment of denture-related inflammatory papillary hyperplasia of the plate.

Twenty patients with denture-related papillary hyperplasia of the palate were surgically treated after careful instruction in oral and denture hygiene, insertion of new dentures and nutritional information. A tissue conditioner was used to cause detumescence of the palatal mucosa presurgically and to act as a wound dressing postsurgically. A knife with a semicircular blade made it possible to cut and plane off the papillary hyperplasia in all regions of the palate and accurately to control and vary the thickness of the extirpated tissue. The advantages of this gentle planning surgery were a short healing time (mean 12 days), no bleeding and no pain that necessitated the use of analgetics. After surgery a persistent erythema of the palatal mucosa appeared in five patients. Such a low rate of postsurgical erythema has not previously been reported. A microscopic examination of the extirpated tissue was performed and the findings are discussed.

Adult↗
PubMed1980

Effect of nystatin in the treatment of denture stomatitis.

The effect of nystatin treatment on denture stomatitis subclassified as generalized simple inflammation and inflammatory papillary hyperplasia has been studied. Twenty-four patients were carefully instructed to apply a thin layer of nystatin dusting powder on the fitting surface of the maxillary denture three times a day for 14 d. Different parameters were studied on three occasions; at the beginning of experiment and after 14 and 28 d. An untreated group of 24 patients with the same types of denture stomatitis served as control. The effect of treatment was evaluated by changes in the clinical erythema, the yeast colonization obtained by mycologic sampling on the palatal mucosa and on the denture base, and changes in the histologic picture of the mucosa obtained by biopsy. Subepithelial inflammation, leukocyte penetration, edema and thickness of the epithelium were histologic parameters. After 14 d of treatment the experimental group compared with the control group showed a statistically significant decrease in palatal erythema and in the amount of yeast colonies. No significant changes were found microscopically. Twenty-eight days after start, i.e. 14 d after withdrawal of the nystatin, there still was a statistically significant improvement of the palatal erythema in the experimental group compared with the controls. However, no patient was totally cured. There were no significant changes in the number of yeast colonies and the histologic parameters.

Aged↗
PubMed1978

Angiolithiasis in the parotid gland.

Angiolithiasis in salivary glands is a very rare finding, and the knowledge of the morphology and histogenesis of these stone formations is therefore limited. Six small angioliths from the left parotid gland of a 19-year-old girl were examined histologically, microradiographically and diffractometrically. The angiolithiasis developed from a regressed hemangioma which had been observed since the patient was 3--4 years old. The angioliths, which were encapsulated, exhibited in some areas diffuse laminations with shells of alternatingly high and low mineral content. In other areas, however, the sturcture of the stones was rather homogeneous. The central part of all angioliths was highly mineralized. All the examined specimens exhibited a mineral pattern of pure apatite. Due to presence of erythrocytes as well as of fibroblasts in the matrix of the examined noduli, the diagnosis of angiolithiasis was established.

Adolescent↗
PubMed1978

Clinical, histologic and micro-radiographic study of natal, neonatal and pre-erupted teeth.

A clinical, histologic and microradiographic investigation of 62 natal, neonatal and otherwise pre-erupted teeth from 51 children is presented. Such a comprehensive material has not previously been reviewed. Of the 62 teeth, 59 were mandibular incisors. A hereditary factor could be demonstrated in 12 cases. Fifty-three teeth showed more or less pronounced enamel hypoplasia. The enamel had a normal prism structure and mineralization with the exception of 22 cases where the prism structure was absent in the cervical part of the enamel. The cervical and apical dentin was atubular and in developing teeth the dentin in these regions changed to an irregularly formed hard tissue of osteodentin character, in which enclosed cells could be observed. Developing teeth often had no cementum, and in those cases where acellular cementum could be observed it was thinner than normal. In four patients atypically located carious lesions occurred.

Child↗
PubMed1978

Ultrastructure of salivary calculi.

Twenty-one submandibular salivary calculi from 19 patients were examined with the light and electron microscope. Adjacent to the peripheral parts of the calculi metaplastic squamous epithelium or connective tissue was seen in close contact to the mineralized matrix. Disintegrated cellular substances from these tissue components were in some cases found to condense in a peripherally located zone of the salivary calculi. The morphology of the examined salivary calculi varied extensively not only within each calculus but also from one calculus to another. High and low incidence of crystals gave rise to the lamellated pattern. In some cases the crystals were so abundant as to five a dense homogeneous appearance. In the central parts of the calculi spheroid bodies of very low electron density with a size ranging from 1 to 100micrometer were found in an amorphous matrix. The origin of these structures is discussed. In the peripheral parts of some calculi osmiophilic spherical bodies with an electron microscopical appearance similar to lipid granules as well as bacteria of cocci or coliform type were found. Degenerated bacteria might in some cases contribute in forming part of the peripheral organic matrix.

Humans↗
PubMed1977

The relation of lipids to the mineral components in salivary calculi.

Using lipid histochemical and microradiographic methods, the distribution of lipids and the inorganic component in different areas of salivary calculi was investigated. Two main groups of lipids were found, i.e. hydrophilic phospholipids and hydrophobic lipids. The hydrophilic phospholipids were shown to be composed mainly of phosphosphingosides and of the hydrophobic lipids cholesterol was demonstrated. Phosphosphingosides were localized in more or less lamellar zones as also in large homogeneous areas. In some cases the calculi were surrounded by a structureless non-mineralized zone exhibiting a strongly positive reaction for phosphosphingosides. Cholesterol was found in isolated band-formed areas. Phosphosphingosides and cholesterol were especially prevalent in areas showing a low or slight degree of mineralization. In areas showing a high degree of mineralization the corresponding lipids gave a weak or absent histochemical reaction. After EDTA-decalcification, however, an increase in the concentration of lipid was noticed in high mineralized areas, indicating a masking process. The influence of various factors on the presence and distribution of different lipids was discussed.

Autoradiography↗