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G Huber

Publications and source records attributed to G Huber.

At least 163 records · Page 9Linked to original sources
PubMed1985

Differences in the developmental patterns of three microtubule-associated proteins in the rat cerebellum.

The developmental distribution patterns of microtubule-associated proteins (MAPs) 1, 2, and 3 were studied using three monoclonal antibodies. Immunochemical staining at the light and electron microscopic levels demonstrated the specific localization of each MAP in different cellular and subcellular compartments. MAP2, which is specifically associated with dendritic microtubules in the adult brain, is strictly associated with growing dendrites from the onset of their formation. MAP3, a recently described MAP of Mr = 180,000, which in the adult brain is associated with neurofilament-rich axons and glial processes, is associated with axons from the beginning of outgrowth. Although MAP3 is not detectable in granule cells and their parallel fiber axons in the mature cerebellum, it does appear transitorily in these axons during development. During neuronal differentiation, MAP1 is found first in axons and only later in dendrites where the highest concentrations are eventually to be found. These results indicate that the combined appearance of MAP1 and MAP2 (dendrites) or MAP1 and MAP3 (axons) correlates with the appearance of morphologically distinct microtubules and provide further evidence that specific MAPs are molecular determinants of dendritic and axonal formation.

Animals↗
PubMed1984

Update and trends in venous (VDSA) and arterial (ADSA) digital subtraction angiography in neuroradiology.

After presentation of the principles and technology of digital subtraction angiography the results of 649 investigations with the peripheral venous method are presented and first experiences are discussed critically. In conclusion the method of venous digital subtraction angiography can be stated to be useful for adequate diagnosis of the extracranial brain vessels. Diagnostic statements about the intracranial vessels by venous injection are limited, and therefore a combined investigation including normal angiography remains necessary. In the near future neuroradiological investigations cannot abandon selective arterial techniques. One may expect however that normal angiographic investigations will subsequently be replaced by digital subtraction angiography with arterial injection of contrast medium.

Angiography↗
PubMed1984

Altered interrelationship of dopamine, prolactin, thyrotropin and thyroid hormone in schizophrenic patients.

Increased dopaminergic activity has been postulated to be one of the main causes of schizophrenia. To evaluate this hypothesis further, the interrelation between dopamine, prolactin, thyrotropin (TSH) and L-thyroxine was studied by determining their concentrations in the serum of ten acutely ill schizophrenic patients exhibiting distinct stages of process activity and ten healthy subjects. The level of dopamine was elevated in the sera of schizophrenic patients, whereas the levels of prolactin, TSH and L-thyroxine were decreased. On the basis of these results we hypothesize that 1. increased dopaminergic activity affects pituitary secretory function, and 2. decreased beta-adrenergic activity may be a consequence of decreased thyroid hormone concentration in plasma.

Adult↗
PubMed1984

Immunocytochemical localization of microtubule-associated protein 1 in rat cerebellum using monoclonal antibodies.

Immunohistochemical staining with monoclonal antibodies showed that microtubule-associated protein 1 (MAP1) has a restricted cellular distribution in the rat cerebellum. Anti-MAP1 staining was found only in neurons, where it was much stronger in dendrites than in axons. There were striking variations in the apparent concentration of MAP1 in different classes of neurons. Purkinje cells were the most strongly labeled, while granule cell neurons gave a faint, threshold-level reaction with the antibody. The reaction of Golgi neurons was intermediate between these two extremes. Equivalent results were obtained using two different methods of tissue preparation. Thus MAP1 appears to be a neuron-specific protein that is highly concentrated in dendrites and occurs at markedly different levels in different types of neurons. These observations provide further indications of heterogeneity among brain microtubules.

Animals↗
PubMed1984

Differences in the cellular distributions of two microtubule-associated proteins, MAP1 and MAP2, in rat brain.

The distribution of microtubule-associated proteins (MAPs) 1 and 2 in rat brain was studied using monoclonal antibodies. Immunochemical staining showed that both MAP1 and MAP2 are present only in neurons and both are highly concentrated in dendrites compared to axons. Otherwise, they differed in distribution in various ways. MAP1 was present at low levels in axons, whereas MAP2 was never detectable in axons with either of two different fixation methods used. In the cerebellum the two MAPs differed in relative concentration in various classes on neurons. Thus, anti-MAP1 staining was strong in Purkinje cells but very faint in granule cells, whereas anti-MAP2 staining was strong in both. There were also distributional differences within the same cell. Thus, in Purkinje cells, anti-MAP1 staining is strong in the cell body, initial axon segment and throughout the dendritic tree, but anti-MAP2 staining is present only in dendrites beyond the initial proximal portion. These results suggest that microtubules with different molecular compositions are present in the cerebellum where they are distributed differently between cells as well as within the same cell.

Animals↗
PubMed1984

[State of the art and developmental trends of venous and arterial digital subtraction angiography in neuroradiology].

After presentation of principle and technology of digital subtraction angiography the results of 649 investigations with the peripheral venous method are demonstrated and first experience are discussed critically. In conclusion the method of venous digital subtraction angiography can be estimated as useful for an adequate diagnosis of the extracranial brain vessels. Diagnostic statements of the intracranial vessels by venous injection are limited, and therefore a combined investigation including normal angiography remains necessary. In the next future neuroradiological investigations cannot renounce from selective arterial techniques. It can be expected that normal angiographic investigations will be replaced by digital subtraction angiography with arterial injection of contrast medium.

Angiography↗
PubMed1983

Fenestration and duplicate origin of the left vertebral artery in angiography. Report of three cases.

Three cases are shown; 2 of angiographically proven distal fenestration of the left vertebral artery and one of duplicate origin. Both anomalies are classified as of inhibitory type. Their incidence in the literature is reviewed. Underlined is the fact that variations of this nature frequently occur in combination with other inhibitory malformations particularly in the vascular and skeletal systems.

Adult↗
PubMed1983

[Behavior of free catecholamines in blood and urine of ambulance men and physicians during quick responses].

Free urine adrenaline, noradrenaline, (additional free plasma catecholamines in the physicians), and blood lactate were determined in 11 ambulance men and 5 physicians to assess stress during medical service. Stress was evaluated employing a stress index, based on difficulties in driving, traffic, severity of injuries or illness. Emergency cases with seriously injured subjects or reanimation were judged to have a 4-fold higher stress index than routine cases where strong physiological or psychological stress was absent. Urine catecholamines and stress indices were estimated in 3-h intervals. The calculations were based on the stress induced catecholamine concentrations minus the basal excretion during the same 3-h interval. Urine adrenaline and noradrenaline in ambulance men and physicians correlated directly with the stress index, as well as the plasma catecholamines of the physicians. Lactate levels showed similar behaviour and a descriptive direct correlation with the plasma catecholamines. Urine adrenaline increased more--dependent on the stress index--than urine noradrenaline. This over-proportional adrenaline response may be an indicator for the additional psychological stress in emergency cases. Therefore physicians showed--based on the same stress index--a tendency to higher urine adrenaline excretion and blood lactate levels than the ambulance men, which might be the consequence of the overall responsibility of the physicians. Because of the observed catecholamine responses during medical service, coronary insufficiency or hypertension might be contra-indications for participation in the medical service; regular clinical investigations including ergometric tests are advisable.

Adult↗
PubMed1983

Long-term prognosis and course of schizo-affective, schizophreniform, and cycloid psychoses.

From the Bonn study including 502 patients followed up an average 22.4 years after onset of schizophrenic disease diagnosed according to the criteria of K. Schneider, we selected 113 cases of schizo-affective, schizophreniform, and cycloid psychoses in accordance to the definitions given by Kasanin, Retterstøl, Angst, and Leonhard. These psychoses have a better prognosis than the whole sample: characteristic residues are seen more rarely, complete remissions and noncharacteristic residues more frequently. This group of psychoses differs from the whole sample in the hereditary taint, too: the morbidity risk with affective psychoses and with schizophrenic psychoses in first- and second-degree relatives is higher than in the total sample of the Bonn study. In spite of the better prognosis and other differences described in the paper, we believe that these results do not justify the classification of schizo-affective and related disorders as an independent disease group. Between these different subtypes of schizophrenia only a differential typology and not a differential diagnostic is possible.

Bipolar Disorder↗
PubMed1983

Susceptibility of naturally occurring carbapenem antibiotics to renal dehydropeptidase-I. An easy assay using a plate technique based on a novel chromogenic beta-lactamase substrate.

A general screening procedure is described which allows the rapid determination of the susceptibility of naturally occurring carbapenem antibiotics to renal dehydropeptidase-I at the broth level. The procedure is based on the incubation of carbapenem-containing solutions with dehydropeptidase-I and the subsequent assay of residual beta-lactamase-inhibiting/inactivating activity of carbapenems by means of a plate technique using the chromogenic beta-lactamase substrate PADAC.

Animals↗
PubMed1983

[Simultaneous determination of hemodynamics and plasma catecholamines in trained and untrained subjects and patients with contraction disorders of the heart during rest and physical activity].

6 trained (TS) and 7 untrained (US) subjects and 16 patients (P) with left ventricular dysfunction were investigated by means of a Swan-Ganz floating catheter during graded bicycle ergometry in a supine position. 9 P suffered from a myocardial infarction and 10 were known to be hypertensive; 10 suffered from acute coronary insufficiency during exercise. Hemodynamic values, free plasma catecholamines, and heart volume at rest were determined in all cases. The relative heart volume was increased training-dependent in the TS as compared with US and P. In TS stroke volume increased more than in US, whereas in P a heart-rate-dependent adaptation of cardiac output was observed during exercise. In P cardiac output was slightly reduced during exercise and the arteriovenous oxygen difference augmented. Systolic and diastolic pulmonary artery pressure, pulmonary wedge pressure, and peripheral diastolic arterial pressure rose more in P during exercise than in TS and US. TS showed reduced, whereas P showed increased levels of circulating free plasma catecholamines (norepinephrine and epinephrine). Training-dependent changes in hemodynamic values and catecholamine levels were seen as an economic adaptation of the heart to physical stress, and the results in the patients were interpreted as indicating an uneconomic stress adaptation. In patients with disturbed left ventricular function, extracardiac compensatory mechanisms such as enhanced sympathetic activity and increased arteriovenous oxygen difference are necessary for the adaptation to physical stress.

Arrhythmias, Cardiac↗
PubMed1983

[Modification of nocturnal heart rhythm disorders using oxygen therapy in patients with chronic obstructive lung disease].

In 14 patients with chronic obstructive lung disease, the effect of nocturnal oxygen therapy was evaluated. The mean flow of 100% oxygen was 2 l/min. We registered the transcutaneously measured blood gases over 10 h, beginning at 8 p.m. while patients were breathing air, the lowest value of PO2 was mean = 45 mm Hg, and while they were breathing oxygen it was mean = 57 mm Hg. Continuous electrocardiograms showed a reduction of supraventricular arrhythmias, from 180 premature beats over 10 h during inhalation of air to 86 during oxygen inhalation. In each of the 13 patients we found a decrease in frequency of premature ventricular beats (from mean = 141 to mean 54 over 10 h, i.e., from 100% to 25.5% +/- 17.1%. During the 2 h of lowest oxygen saturation, the frequency of ventricular arrhythmias was increased. We expected 20% and registered 38.8%.

Arrhythmias, Cardiac↗