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Biomedical subjects

G Hall

Publications and source records attributed to G Hall.

At least 199 records · Page 11Linked to original sources

Dissipation of parathion and paraoxon on citrus foliage dust and dry soil surfaces in a treated orchard.

Pesticides applied to an orchard may be transported via dust from the orchard floor to the foliage. The dust containing the pesticide facilitates oxygen analog formation. This study involves the evaluation of existing methods and new procedures for measuring pesticide fate (parathion and paraoxon) on soil surfaces in a field environment; the results were correlated with dislodgeable foliar residues. Residues in soil dust removed from the ground floor via vacuum varied considerably and did not correlate with dislodgeable foliar residues. Although residues found on masonite plates coated with a thin layer of soil were different from dislodgeable foliar residues, the ratios of paraoxon to parathion was very similar for the first 14 to 21 days. The thin-layer soil on the plates had serious deficiencies including difficulty of preparation in the field, handling of the coated plates, and physical movement of the soil from the plate due to environmental forces including rain. Soil tags prepared from fine mesh window screen saturated with soil resulted in approximately the same residue as the soil plates and at the same time avoided most of the deficiencies experienced with the plates. They were very resistant to physical forces, easy to prepare in the field, and not affected by environmental forces except appreciable quantities of rain. The results should encourage the use of soil screen tags, not in lieu of but along with other methods when conducting field studies with pesticides involving worker safety.

Citrus↗

Furazolidone in turkey tissues following a 14-day feeding trial.

Feed supplemented with furazolidone was fed to turkeys on a research farm near Modesto, CA. The birds fed furazolidone-medicated feed were housed in isolated pens in a manner to prevent any cross contamination from an adjoining treatment. Furazolidone-medicated feed was supplied to the ration for 14 days prior to withdrawal with two exceptions; the controls were not fed medicated feed, and a 400 g/ton treatment was fed for 24 hr prior to processing. Treatments, representing different withdrawal periods, ranged from 0 to 21 days. Two 400 g/ton treatments with 0-day withdrawal periods were included in the study. One of these treatments involved a 14-day medicated feeding period while the other was for 24 hr. All other treatments were fed medicated feed at the rate of 200 g/ton. Tissue samples from the processed birds included skin, fat, liver, kidney, as well as breast and thigh muscle. No detectable residues were found in any of the liver, kidney, fat, or muscle tissues at any of the withdrawal periods including the 0-day withdrawal groups. Skin tissues contained detectable furazolidone residues only in the 0-day withdrawal treatments, and even these levels were below the 2 ppb level.

Administration, Oral↗

Comparison of nail-plate fixation and Ender's nailing for intertrochanteric fractures.

Two comparable groups of patients with fractures of the trochanteric region of the femur were studied. One hundred and sixty-five fractures had been fixed with conventional nail-plates and 132 with Ender's nails. Ender's nails gave superior results in the treatment of trochanteric fractures. The operation was quicker and less traumatic than that using conventional nail-plates and both the mortality rate and the time in hospital were reduced. More patients in the group with Ender's nails who were initially independent returned to an independent existence.

Adult↗

Ultra trace determination of furazolidone in turkey tissues by liquid partitioning and high performance liquid chromatography.

A simple and sensitive procedure is presented for the determination of furazolidone in turkey tissues, using liquid partitioning followed by high performance liquid chromatography (HPLC). Fat, liver, kidney, skin, and muscle tissues are ground with methylene chloride in a Polytron homogenizer, followed by solvent removal, partitioning in hexane-0.01M acetic acid, and back-partitioning the 0.01M acetic acid with methylene chloride. The determination by HPLC used a reverse phase Ultrasphere-ODS 5 micrometer column. The method is sensitive to 0.5 ppb, with a standard deviation of 6.39% at the 2 ppb fortification level. Recovery from fortified tissues averaged 84% from samples fortified with 0.5-10 ppb furazolidone. An alternative cleanup procedure using a Sep-Pak C18 cartridge is also presented.

Animals↗

Facilitation and retardation of discrimination learning after exposure to the stimuli.

In three experiments, rats was required to learn a simultaneous discrimination in a jumping stand between horizontally and vertically striped objects. Experiment 1 showed that prior prolonged exposure to these stimuli in the rats' home cages helped them to learn the discrimination. Experiment 2 showed that a briefer period of exposure (1 hr per day for 50 days) was equally effective when the stimuli were presented in the home cage but produced a retardation of discrimination when the stimuli were presented in the jumping stand itself. Experiment 3 demonstrated that prior exposure to the jumping stand was not in itself enough to produce a retardation of subsequent discrimination learning. Some implications of these results for current theories of perceptual learning and latent inhibition are discussed.

Animals↗

Approach to recognition of regulatory mutants of cyanobacteria.

Antimetabolite analogs of essential amino acids are useful as selective agents for isolation of regulatory mutants of cyanobacteria, although we observed striking microbiological differences from other widely used eubacterial systems. Regulatory mutants shown to overproduce and excrete tryptophan, phenylalanine, tyrosine, methionine, or arginine were isolated from four cyanobacteria: Anabaena sp. 29151, Synechococcus sp. 602, Synechococcus sp. AN Tx20, and Synechocystis sp. 29108. Surprisingly, regulatory-mutant colonies did not support a halo of cross-fed wild-type growth on selective medium. Since regulatory mutants were shown to excrete substantial levels of amino acids, it was deduced that poor cross-feeding must reflect a generally low nutritional responsiveness of the cyanobacterial background. This conclusion was confirmed by results which showed that regulatory-mutant cells of cyanobacteria dispersed among wild-type populations of Bacillus subtilis did produce halo colonies on solid analog-containing medium. Cross-feeding between one cyanobacterial pair (a phenylalanine excretor and a phenylalanine auxotroph) was successfully demonstrated in the absence of the analog under conditions in which relatively large masses of each cell population type were spread near one another on agar plates. These results suggest that amino acid excreted by regulatory mutants of cyanobacteria on analog-containing selective medium is transported into nearby wild-type cells too inefficiently to overcome the antimetabolite effects of the analog, thereby failing to generate halos of physiologically resistant background cells. Consistent with this interpretation was the finding that the pheA1 auxotroph from Synechococcus sp. 602 exhibited a linearly proportional dependence of growth rate upon exogenous concentration of l-phenylalanine (below 20 muM). Wild-type B. subtilis serves as a convenient and sensitive test lawn for screening obvious regulatory mutants from among collections of analog-resistant cyanobacterial mutants. Appropriate B. subtilis auxotrophs can be used as convenient indicator strains for the identification of regulatory mutants in cyanobacteria through the observation of syntrophic growth responses.

Amino Acids↗

Behavioral management of exercise: contracting for aerobic points.

Behavioral contracting was used to encourage physical exercise among college students in a multiple-baseline design. Subjects deposited items of personal value with the experimenters, which they could earn back on fulfillment of two types of contract contingencies. Subjects selected weekly aerobic point criteria, which they could fulfill by exercising in the presence of other subjects. In addition, subjects contracted to observe and record the exercise of other subjects and to perform an independent reliability observation once each week, with both of these activities monitored by the experimenters. Results indicated that the contract contingencies produced increases in the number of aerobic points earned per week for seven of eight subjects, that the aerobic point system possesses several advantages as a dependent variable for behavioral research on exercise, and that inexperienced observers could be quickly trained to observe exercise behavior and to translate those observations into their aerobic point equivalents. Finally, in a followup questionnaire completed 12 months after the end of the study, seven of the eight subjects reported that they were earning more aerobic points per week than had been the case during the baseline condition of this experiment.

Adult↗

Loss of associability by a compound stimulus comprising excitatory and inhibitory elements.

In each of two experiments rats learned a discrimination between a stimulus (A) that signaled shock and a compound stimulus (AB) that signaled no shock. In Experiment 1 it was found that the AB compound acquired excitatory strength only slowly when, in a second phase of training, it was made to signal the occurrence of shock. In Experiment 2 the acquisition of inhibitory strength by the compound was similarly found to be retarded. This second experiment also replicated the results of Experiment 1. The relevance of these results to current theories of latent inhibition and attention is discussed.

Acoustic Stimulation↗

Latent inhibition of a CS during CS-US pairings.

In each of three experiments rats were trained by the conditioned-emotional-response technique with a conditioned stimulus (CS) predicting a relatively weak shock, the unconditioned stimulus (US). In the second stage of training the intensity of the shock was increased, and it was found that subjects for whom the same CS was used in both stages acquired further suppression less readily than subjects that experiences a new CS in the second stage. The implication of these results for theories of attention and for theories of habituation is discussed. It is suggested that associations formed by the test CS during the first stage of training reduce the readiness of the stimulus to enter into new associations, either because an association between the stimulus and the context reduces further processing of the stimulus or because the association between the test stimulus and the weak shock attenuates the formation of an association with the stronger shock.

Animals↗

Thyroid hormone kinetics: improved method for quantitative separation and measurement of the various radioiodothyronine injection.

An accurate and reproducible method for measurement of radioactive species in blood after in vivo injection of labelled iodothyronines is described. By extraction with high-affinity antisera, radioactive reverse T3 and T3 are separated from serum quantitatively. Radioiodide is quantitatively separated from radio-thyronine species and serum proteins by Sephadex G50 filtration. The residual mixture of radio-T4 and iodoprotein is quantitatively resolved by ion-exchange adsorption. Minimal misclassification of radiospecies occurs, and can be corrected for. Mean recoveries of various radiospecies added to serum were: radioiodide 98.9%, radio-rT3 87.6%, radio-L-T3 94.5%, radio-T4 98.0% and radioiodoprotein 94.5%. The performance of the method is superior to that of chemical methods such as trichloracetic acid precipitation, ion-exchange or alkaline Sephadex extraction, and chromatographic separation.

Chromatography, Gel↗

The outcome of adolescent school phobia.

A hundred-and-twenty-five school phobic youngsters had been treated in a psychiatric in-patient unit for young adolescents of secondary school age over a seven-year period; a hundred of them were reviewed on average three years after discharge. About a third of cases were found to have improved little; they had persistent severe symptoms of emotional disturbance and continuing social impairment. Another third had improved appreciably and were affected by neurotic symptoms rather than social impairment. The remaining third had improved substantially or completely. School attendance difficulties had remained in about half of all cases. Subsequent difficulties in going to work were less pronounced. The best predictor of outcome was clinical state on discharge. High intelligence also emerged as a significant predictor of poor outcome. Five girls and a boy had already developed severe and persistent agoraphobic difficulties when reviewed. It was found that severe school phobia in early adolescence resembled adult affective disorders in some clinical features and in outcome.

Absenteeism↗