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Biomedical subjects

G Fex

Publications and source records attributed to G Fex.

At least 73 records · Page 4Linked to original sources
PubMed1981

Isolation and partial characterization of a low molecular weight trypsin inhibitor from human urine.

A low molecular weight glycoprotein which completely inhibited trypsin at a 1 : 1 molar ratio was isolated from human urine. It was generated from a precursor molecule which in turn derived from plasma inter-alpha-trypsin inhibitor. It had one polypeptide chain with a molecular weight of about 20 000 and a high content of half-cystine residues. Its amino-terminal amino-acid sequence was Val-Thr-Glu-Val-Thr-X-Leu-Glu-Asp-.

Alpha-Globulins↗
PubMed1981

Serum ferritin, gammaglutamyl-transferase and alcohol consumption in healthy middle-aged men.

Serum ferritin was analysed in 169 middle-aged men. Ninety-one were heavy drinkers with increased serum gammaglutamyltransferase (GGT) values in otherwise normal routine screening tests; 39 were from the same birth year cohorts with normal GGT values; and 39 were teetotalers. Increased ferritin values were found in 67% of the heavy drinkers, but only in 2.5% in the two other groups. The mechanisms of the serum ferritin elevation in otherwise healthy alcohol over-consumers are unknown, but heavy drinking should be taken into consideration in the interpretation of increased serum ferritin values in individual cases.

Alcohol Drinking↗
PubMed1981

Oestrogen-like effects of tamoxifen on the concentration of proteins in plasma.

The concentrations of 11 plasma proteins were determined by electroimmunoassay in 6 female and one male patient before and after treatment for 1-4 months with tamoxifen (30 mg/day) for advanced cancer. The concentrations of transcortin, sex hormone binding globulin, pregnancy zone protein, apolipoprotein AI and thyroxine binding globulin increased significantly. The concentrations of orosomucoid and haptoglobin decreased significantly. The concentration of albumin, transferrin, prealbumin and retinol-binding protein showed no significant change. The changes after treatment with tamoxifen were thus similar to those which occur after the administration of oestrogens or during pregnancy although they were quantitatively less pronounced. There were also differences in the pattern of change between treatment with tamoxifen and during pregnancy. There was no apparent relationship between response to treatment and changes in concentration of plasma proteins.

Adult↗
PubMed1980

Alcohol consumption and premature death in middle-aged men.

All the men living in Malmö born in 1926-9 were invited for a screening examination which included an assessment of alcohol consumption and measurement of gamma-glutamyltransferase (GGT) activity. They were followed for up to four years (median 2) and their mortality assessed. Sixty-two deaths occurred, 41 (0.9%) among the 4571 men who attended the screening investigation and 21 (1.3%) among the 1609 who did not respond to the invitation. Evidence of alcohol abuse or an alcohol-related cause of death was present in 25 (61%) of the deaths among the attenders and 13 (62%) of those among the non-responders. GGT values at the screening investigation were significantly increased in 19 (46%) of those who died, but established risk factors, such as cholesterol and triglyceride concentrations and blood pressure, had little predictive value. Measurement of GGT provided an objective index of alcohol consumption, though the full clinical importance of a raised value needs further assessment. The finding that heavy alcohol consumption was the single most important factor associated with premature death in these middle-aged men has important implications for prevention.

Alcohol Drinking↗
PubMed1980

Plasma prealbumin concentration in alpha 1-antitrypsin deficiency (PiZ).

The concentrations of 7 plasma proteins including prealbumin, retinol-binding protein, and alpha 1-antitrypsin were measured by electroimmunoassay in plasma from 19 persons with alpha 1-antitrypsin deficiency PiZ, 20 persons with phenotype PiMZ, and 26 healthy men with normal alpha 1-antitrypsin content. The prealbumin concentrations in the PiZ individuals were slightly lower than in the other groups. Only one individual in the PiZ group showed very low levels of prealbumin and retinol-bonding protein. Thus contrary to what has been proposed earlier our results show that severe prealbumin deficiency is not a common finding in alpha 1-antitrypsin deficiency PiZ.

Adolescent↗
PubMed1980

HDL-increasing effect of cyclofenil.

Nineteen patients with low levels of HDL as measured by APO-AI have been treated with cyclofenil (a non-steroid stilboestrol isomer with very low oestrogenic effects) as the only form of therapy. The therapy elicited a highly significant rise of about 15% in HDL levels. A slight increase in transaminases occurred in 6 patients but was reversible without terminating therapy.

Cholesterol↗
PubMed1980

Serum gamma-glutamyl transferase and a somatic health score in middle-aged men.

In an ongoing population investigation of middle-aged men in Malmö, Sweden, several health screening variables showed strong but crude individual covariations with the level of the hepatic enzyme, serum gamma-glutamyl transferase (GGT). These variables were combined, according to an analysis of their normal distributions, into a score index which exhibited a much smoother correlation with low, normal, and elevated levels of GGT when tested in a random population subsample. It is concluded that this scoring system may find further utilisation as a general descriptive method of recording statistical covariations between health screening tests and sum them up.

Adult↗
PubMed1980

Subunit exchange between human and dog prealbumins.

When human prealbumin is mixed with carb,mylated human prealbumin or dog prealbumin hybrid prealbumins containing subunits of both types are formed. Spontaneous hybrid formation was a slow process. By renaturation from 6 mol/l quanidine hydrochloride, hybrids, distributed according to random combination of subunits were formed. The hybrids were stable, had a molecular weight similar to their parent molecules and bound thyroxine and retinol-binding protein. Only the hybrids with human and dog prealbumins participated in thioldisulphide exchange reactions as their parent molecules. The ability of prealbumin to exchange subunits of low molecular weight may be the reason why prealbumin concentration in cerebrospinal fluid is higher than expected from its plasma concentration and molecular weight.

Animals↗
PubMed1980

Effect of angiographic contrast media on the liver. I. Serum concentration of enzymes after coeliacography with balloon catheter in the pig.

In pigs serum concentrations of ASAT, ALAT, gamma GT, AP and amylase were determined during one week after coeliacography balloon catheter for temporary arterial occlusion. One group of pigs was injected with iohexol (non-ionic contrast medium) and another group with metrizoate (ionic contrast medium) and another group with metrizoate (ionic contrast medium). In a control group metrizoate was injected into the lumbar artery. Small and transient increases in serum concentrations of hepatic enzymes and amylase were recorded 180 min to one day after the injection in all 3 groups. No significant differences in enzyme reactions between the groups were observed.

Alanine Transaminase↗
PubMed1979

Retinol-binding protein from human urine and its interaction with retinol and prealbumin.

In freshly collected urine from a patient with glomerulotubular proteinuria there were two bands which contained retinol-binding proteins. The cathodal band showed fluorescence in the ultraviolet. After extraction with organic solvents only the anodal non-fluorescent band remained. After addition of an excess retinol only one band remained which by mobility corresponded to the cathodal band. The anodal of the two bands was therefore probably the apo form and the cathodal the holo form of the same retinol-binding protein. Their proportions, determined by densitometric scanning were approximately 4/1 (anodal/cathodal band). More than 85% of the retinol-binding protein in the urine bound to prealbumin-Sephrose. The apo retinol-binding protein from urine had the same electrophoretic mobility on agarose gel el-ctrophoresis and the same pattern on isoelectric focusing as an retinol-binding protein prepared from serum. The carboxy-terminal amino acid sequence of the retinol-binding protein from freshly collected urine that bound to prealbumin-Sepharose, was -Arg-Leu. The amino-terminal sequence was Glu-Arg-Asp-Cys-Arg-Val-Ser-X-Phe-Arg-Val-Lys-Glu-Asn-Phe-Asp-Lys-Ala-Arg-Phe-X-Gly-Thr-Trp-Tyr-. This sequence and the amino acid composition are compatible with the view that the retinol-binding protein in urine is the same as in plasma.

Amino Acid Sequence↗
PubMed1979

Interaction between prealbumin and retinol-binding protein studied by affinity chromatography, gel filtration and two-phase partition.

The interaction between prealbumin and apo or holo retinol-binding proteins has been studied by affinity chromatography, gel filtration and two-phase partition. At physiological ionic strength apo and holo retinol-binding protein form 1:1 molar complexes with prealbumin. Mean dissociation constants for the prealbumin compex with apo retinol-binding protein and holo retinol-binding protein with all-trans retinol, retinoic acid, retinal and retinyl acetate were calculated from the partition data as 0.33 +/- 0.11 x 10(-6) M and 0.075 +/- 0.015 x 10(-6) M respectively (mean +/- S.E.M.). The difference was statistically significant. Quantitative data on the amount of retinol, retinol-binding protein and prealbumin in plasma and urine were in good agreement with the ratio of the dissociation constants for the complexes of apo and holo retinol-binding proteins with prealbumin as determined in the partition experiment. The magnitude of the dissociation constants was compatible with previously published data on the turnover of retinol-binding protein.

Aged↗
PubMed1978

Purification of retinol-binding protein from serum and urine by affinity chromatography.

A new method for the purification of retinol-binding protein from urine and serum is described. The method is based on the reversible binding of retinol-binding protein to retinoic acid linked to Sepharose and prealbumin linked to Sepharose. The yield is comparable to conventional methods using ion exchange and gel filtration and the product is an apo retinol-binding protein of high purity. The product has the same electrophoretic mobility and molecular weight and the same ability to interact with retinoic acid and prealbumin as retinol-binding protein prepared by conventional methods.

Chromatography, Affinity↗
PubMed1978

Changes in plasma high density lipoproteins in chronic male alcoholics during and after abuse.

Alterations in plasma high density lipoproteins (HDL) were studied in thirty-eight male chronic alcoholics. Twenty-four (63%) of the patients had increased HDL protein (measured immunochemically) and twenty-five (66%) had increased HDL cholesterol (determined after polyanion precipitation of very low density lipoproteins (VLDL) and low density lipoproteins (LDL)). A statistically significant correlation was found between HDL protein and HDL cholesterol (r = 0.39). gamma-Glutamyltransferase (GT) was elevated in eighteen (47%) of the alcoholics. No significant correlations were found between GT and HDL protein or HDL cholesterol. The increase in HDL, as studied by rate zonal ultracentrifugation, was heterogeneous with changes in the HDL2 as well as HDL3 subfractions. It is suggested that determination of HDL total cholesterol, in combination with GT, may represent a valuable and sensitive test for detection of alcoholism.

Adult↗