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Biomedical subjects

G E Moore

Publications and source records attributed to G E Moore.

At least 181 records · Page 10Linked to original sources
PubMed1979

Failure of spleen repair.

A 25-year-old man underwent splenorrhaphy for a laceration of the inferior pole following blunt trauma. After 5 weeks of uneventful convalescence re-exploration was necessary for delayed formation of a perisplenic hematoma. We conclude that spleen repair is justified in the adult patient with simple injury to the poles of the organ. Careful selection and followup are essential for patients who undergo splenorrhaphy.

Adult↗
PubMed1979

Comparison of four new cell lines from patients with adenocarcinoma of the ovary.

Permanent human tumor cell lines COLO 110, COLO 316, COLO 319, and COLO 330 were established from four patients with serous cystadenocarcinoma of the ovary. COLO 110 was derived from primary tumor tissue; COLO 316, COLO 319, and COLO 330 were derived from cells in malignant effusions. COLO 110 and COLO 316 grew as monolayers of epithelioid cells in culture; COLO 319 and COLO 330 grew as vermiform, floating colonies of epithelioid cells in culture. Epithelial-like morphology was confirmed by transmission electron microscopy. All four cell lines had marker chromosomes and double minute chromosomes. Giemsa banding revealed chromosomes 1, 3, 6, and 7 were involved in markers in all four lines, and chromosomes 2, 4, 5, 9, 11, and 15 were involved in markers in three of the cell lines. Marker chromosomes with possible homogeneous staining regions were observed in COLO 319. Estrone was elaborated by three of the lines, but neither chorionic gonadotropin, carcinoembryonic antigen, nor estrogen or progesterone receptor proteins were detected. Each cell line demonstrated a distinctive isozyme phenotype. These cell lines are maintained in active culture and in a cell bank for distribution to other investigators.

Aged↗
PubMed1979

Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions.

Two human colon carcinoma cell lines derived from the same tumor specimen were characterized. The cell lines, COLO 320 and COLO 321, have amine precursor uptake and decarboxylation cell properties, such as ectopic production of norepinephrine, epinephrine, serotonin, adrenocorticotropic hormone, and parathyroid hormone. The cells were morphologically different from most colon cell lines. Double minutes (DM) were initially present in nearly 100% of the metaphases. In a few subcultures of COLO 320, DM have persisted for 1.5 years. However, in COLO 321 and some subcultures of COLO 320, a loss of DM was observed and new marker chromosomes with homogeneously staining regions were observed. These unusual cell lines should be valuable for studies of apudomas of the colon and the cytogenetic phenomena of DM and homogeneously staining regions.

Animals↗
PubMed1978

Leukemic reticuloendotheliosis. Loss of platelet defect after splenectomy.

A 59-year-old woman with leukemic reticuloendotheliosis had pancytopenia and hypersplenism. Before splenectomy, she had an abnormal bleeding time and a severe defect of secondary-wave platelet aggregation. After splenectomy, the platelet count returned to normal, and the aggregation defect was no longer present. This response suggests that the spleen may contribute to the platelet dysfunction.

Blood Platelets↗
PubMed1978

Condyloma. A new epidemic.

Condyloma acuminatum of the anus is an increasingly frequent disease that may require a combination of therapies. Large bulky lesions can be reduced with topical freezing, cautery, or the scapel. Topical chemotherapy with podophyllum resin can be supplemented with cancer chemotherapeutic agents. Many of these drugs probably act, at least in part, by stimulating a strong local cellular immune reaction. Dinitrochlorobenzene was used to eradicate lesions that had been resistant to treatment with podophyllum resin. 5-Flourouracil was used as a control. All 23 patients studied were initially skin tested with both agents and randomized to either treatment group. All but one of the dinitrochlorobenzene-treated patients had excellent regressions. Immunotherapeutic agents, such as dinitrochlorobenzene, warrant further study as adjuvant therapeutic agents for this disease.

Antineoplastic Agents↗
PubMed1978

A transitional cell carcinoma cell line.

A transitional cell carcinoma cell line, COLO 232, was derived from a primary urinary bladder tumor in a Caucasian male. In culture, COLO 232 retained distinct uroepithelial phenotypic traits and produced both carcinoembryonic antigen and adrenocorticotropic hormone. COLO 232 had a chromosome mode of 58 and retained the X and Y chromosomes. Ten marker chromosomes were identified. COLO 232 will be of value for biochemical and immunological studies.

Adrenocorticotropic Hormone↗
PubMed1978

Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model.

Three tumor cell lines (COLO 201, COLO 205, and COLO 206) have been established from ascites fluid obtained from a male patient with adenocarcinoma of the colon. In addition to the tumor lines, two lymphoid lines (COLO 197 and COLO 200) have been established from the same patient, with one line from the original biopsy and one from peripheral blood. Characterization of the tumor cell lines revealed four cell types that differ from most colon cell lines reported by others. Chromosome markers were identical in COLO 201 and COLO 205. A long-arm isochromosome 5 observed in COLO 201 and COLO 205 was absent in COLO 206. Statistical analysis of autosomal polysomy revealed that these cell lines were stable and indicated that there may be a cytogenetic basis for the three predominant types of cell morphology. The lymphoid cell line derived from the peripheral blood had a normal male karyotype. The lymphoid cell line derived from a biopsy specimen had a mode of 46 and a deleted chromosome 7 marker. Both lymphoid cell lines had B-cell characteristics. These autochthonous cell lines have been used for immunological studies in cytotoxicity assays and immunoglobulin characterization.

Adenocarcinoma↗
PubMed1977

Marker properties of tumor and lymphoid cell lines derived from a patient with squamous cell carcinoma.

A squamous cell carcinoma tumor cell line, COLO 227, derived from a metastatic tumor in a Caucasian male, produces both parathyroid hormone and carcinoembryonic antigen. A chromosome mode of 106 predominated and the X and Y chromosomes were retained. Seven marker chromosomes were identified. Cytogenetic analysis revealed an isochromosome 8 marker similar to a HeLa cell line marker and an isochromosome 17 marker described in other cancers. An autochthonous lymphoid cell line, COLO 219, was established and characterized. COLO 219 is a normal lymphoid cell line with B-cell characteristics. This autochthonous system of both cultured tumor cells and cultured lymphocytes is of use in immunological studies.

B-Lymphocytes↗
PubMed1976

Feasibility study of active immunotherapy in patients with solid tumors.

Forty-five immunocompetent patients with solid tumors were immunized with BCG, PPD, and tumor cells. The methods were practical, but the morbidity was significant, including painful draining ulcerations at vaccine sites, possible enhancement of tumor growth in three patients, and the discovery at autopsy of systemic tuberculosis in one patient. Various facets of cellular immunity were altered, namely: 1) a majority of the patients developed enhanced cutaneous reactions to the microbial skin-test antigens (particularly tuberculin) and tumor cells; 2) nine patients developed the equivalent of delayed hypersensitivity reactions or flares at all previous PPD and BCG inoculation sites following subsequent injection of these agents, which supports the concept of immunologic memory for these target antigens; 3) lesions resembling those of "spontaneous" regressed moles (halo-nevi) were observed at previous vaccine sites in 20 patients and generalized depigmentation occurred in three patients; 4) foreign body giant cells in tumor metastasis remote from BCG-PPD-tumor vaccine sites may indicate a cross-reactivity of microbial and tumor antigens; and 5) intralesional inoculation of the nonspecific agents (BCG, PPD, Varidase, and Mumps) resulted in dense mononuclear cell infiltration and complete regression of most of the injected lesions. Destruction of single or multiple lesions by local injections of antigens did not provide either significant regression of uninjected lesions or clinical benefit.

Abscess↗