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Biomedical subjects

G Corradi

Publications and source records attributed to G Corradi.

At least 37 records · Page 2Linked to original sources
PubMed1990

Application of monoclonal antibodies in diagnosis of human breast cancer by fine-needle aspiration cytology.

In the present study the authors have examined 100 breast nodules by fine-needle aspiration cytology (FNAC) using the avidin-biotin complex method of immunoperoxidase staining. The monoclonal antibodies (MAbs) employed, B 6.2 and B 72.3, recognize two distinct molecules expressed by transformed breast epithelium. The authors have compared this immunocytochemical reaction with the conventional cytology and the histological examination as well. The data of the MAbs alone or in combination have been evaluated in terms of sensitivity, specificity, predictive value and test efficiency. The results show that tumor cells are strongly reactive with the MAbs. So the authors believe that this method in conjunction with conventional cytology may be useful in the diagnosis of breast tumor, especially in doubtful FNAC.

Antibodies, Monoclonal↗
PubMed1990

Clinical value of a new tumor marker (M.C.A.) in the breast cancer follow-up.

The authors evaluated the levels of a mucinous-like carcinoma antigen (M.C.A.) in the serum of 105 follow-up patients with breast carcinoma by means of an enzyme immunoassay. The negative/positive cut-off level determined in a group of healthy women was 13.2 U/ml. 80 patients turned out to be free disease, while 25 patients showed relapse or metastasis. High levels of MCA were observed in 72.0% of the patients with renewal of the neoplastic disease as well as in 12.5% of the free disease subjects. The EIA method appeared to have a good specificity (87.5%) and a fair test efficiency (83.8%). On the contrary, its sensitivity (72.0%) and its predictive value (64.3%) were not completely satisfactory. Therefore we think the matter needs to be studied more deeply and so does the case-report.

Adult↗
PubMed1990

Thymostimuline and uterine cancer satellite lymph nodes. Morphometric comparison between an 8-day-before-surgery and a 14-day-before-surgery single treatment.

The Authors analyse the functional areas (cortical, paracortical, germinal center, medullary, histiocytosis of the sinuses) of regional lymph nodes in women having uterine carcinoma and previously treated with Thymostimuline, and who were operated on respectively 8 and 14 days after inoculation. They also evaluate the degree of significance when compared with a control group treated locally with a physiological solution. The number of lymph nodes examined is at least 5, and the stereological analysis was carried out with the use of a grid: 200 points, actual area 200 cm2, effective area at the level of the preparation 0.32 mm2 (250 X). The Authors examine the results obtained after 8 days' action by Thymostimuline, and, after 14 days, the difference between the 8-day-after and the 14-day-after administration results, and those between the cervical and the endometrial. 4 women with uterine cancer (Stages I and II) and treated with the same doses of physiological saline solution were used as controls.

Carcinoma, Squamous Cell↗
PubMed1989

Morphometric analysis of peritumoral lymph nodes in patients operated on for uterine cancer, locally treated with a thymic extract.

By means of a morphometric analysis, the authors have evaluated the structure and dimensions of the lymph node functional areas (cortical, medullary, paracortical, histiocytosis of the sinuses, germinal centres) in regional nodes of women with carcinoma of the uterus in the 1st and 2nd stages. Twenty patients were treated 8 days before surgery with a dose of 1.5 mg kg-1 body weight of thymic hormone directly into the uterine portio. Ten patients, on the other hand, were injected with physiological saline solution. The quantitative and qualitative results show that in peritumoral lymph nodes of women treated with thymostimolin there is a marked increase, statistically significant (P less than 0.001), of the paracortical zone and of the number of germinal centres.

Female↗
PubMed1989

The occurrence of Chlamydia trachomatis in the semen of men participating in an IVF programme.

Chlamydia trachomatis is one of the most common causative agents of sexually transmitted diseases. The authors studied the occurrence of C. trachomatis in the semen of 184 asymptomatic men participating in the IVF programme. Twenty-six (14.1%) of the 184 tested were positive for C. trachomatis, these patients and their wives receiving doxycycline capsules twice, 100 mg on the first day and 100 mg/day for the following 13 days. This treatment was effective in 88.5% of the cases and in the rest, treatment continued with erithromycin 250 mg four times/day for 2 weeks. The authors compared the semen parameters (cell count, motility, morphology, bovine mucus penetration and hypo-osmotic swelling test) in the infected and non-infected groups and observed no significant difference between these two groups.

Chlamydia Infections↗
PubMed1989

Steriological analysis of peritumoral lymph nodes in patients operated for cervical and endometrial cancer after being treated with thymostimulin.

We have evaluated, by means of stereological analysis, the dimensions of the lymph node functional areas (cortical, medullary, paracortical, histiocytosis of the sinuses, germinal centers) in regional lymph nodes of women with cervical cancer (15 women) and endometrial carcinoma (6 women) in Ist and IInd stage, treated 8 days before surgery with a dose of 1-1.5 mg/kg of body weight of thymostimulin directly into the uterine cervix. 4 women with cervical and endometrial cancer (stages I and II) and treated with the same doses of physiological saline solution were used as controls. The quantitative results show that in the lymph nodes of women treated with thymostimulin there is a statistically significant marked increase (P less than 0.001), of the paracortical areas.

Female↗
PubMed1989

Melanoma relapse of the upper third of the vagina treated with BCG and beta-interferon.

Faced with a vaginal melanoma relapse in an old cardiac patient operated in May 1985 for melanoma in the upper 3rd of the vagina, we had to solve a therapeutic problem in view of the impossibility of excising a lesion larger than the preceding one and because the patient refused every type of surgery. So we then chose the combined treatment of intralesional BCG and intravenous beta-Interferon. Monthly intravenous administration of beta-Interferon has reached a total of 8,000,000 I/U, while the BCG has been inoculated intralesionally in the submucosa in doses of 0.1 ml after observing the negative result of the PPD test. Our aim was to achieve double immunological stimulation by combining two drugs that are often used separately with satisfactory results. Before and after every therapy cycle, the immunological status was established, and showed a small but constant and permanent increase in T-lymphocytes, both helper and suppressor. Histological results before and after therapy and immunological responses are reported, and the case distinguishes itself by its brilliant results.

Aged↗
PubMed1987

[Immune complexes as markers in breast cancer and melanoma].

During the year 1985 the Authors, using three-monthly blood abstractions, monitored 67 patients affected with carcinoma in order to observe the serum levels of immune complexes using two enzyme immunoassays. They compared the results obtained by the two methods in connection with the type of tumor and moreover they attempted to find a correlation with the neoplastic progression. The final results are nevertheless little encouraging in the use of these methods to observe the development of the disease.

Adult↗
PubMed1986

[Quantitative analysis of lymphocyte and monocyte levels in patients after operations for breast cancer].

The much less than Quantigen T and B Cell Assay much greater than method is used to evaluate T and B lymphocyte levels, Nuls Cells and monocytes in the peripheral venous blood of patients surgically treated for breast cancer. The results obtained show a change in immune competent cell levels and temporary immune restoration of the immune response during treatment with immunostimulators.

B-Lymphocytes↗
PubMed1986

[Scanning electron microscopy study of changes induced by thymomodulin on the morphology of mononuclear elements of peripheral blood from healthy subjects and patients with neoplasms].

The authors studied with scanning electron microscopy the surface of peripheral mononucleated blood cells of healthy subjects and neoplastic patients before and after incubation with thymomodulin, a calf thymus derivative with immunomyelomodulating activity. In the healthy subjects before incubation with thymomodulin a wide number of lymphocytes with villous surface was observed, while monocytes appeared as bigger cells with a central portion of cytoplasm with an adhesion plate. Already after an hour of incubation with the thymic derivative an increase of rough lymphocytes and the appearance of cytoplasmic bridges between lymphocytes and monocytes were observed. Afterwards thymomodulin induced in lymphocytes the forming of cytoplasmic appendages named "uropods" and "pseudopods" which are expression of lymphocytic activation. In cancer patients at base line "monstrous" cells, considered as suffering lymphocytes, were seen and they became morphologically normal after incubation with the thymic derivative.

Adult↗
PubMed1985

The action of interferons on the morphology of mononuclear elements of human peripheral blood. S. E. M. observations.

Scanning electron microscopy has been used to study the surface of lymphocytes and monocytes before and after treatment with interferon in vitro. Aliquots of peripheral blood isolated cells have been tested using either human leucocytic interferon (HuIFN-alpha) and human fibroblast interferon (HuIFN-beta) and both types combined. The investigation has been performed at various incubation times. The freshly isolated untreated lymphocytes exhibit a rough and villous surface. The smooth forms are uncommon. The treatment by various interferon types induces a new distribution of villous and rough structures as well as the forming of new elements called "uropods" and "pseudopods". At the same time, the binding of lymphocytic elements is enhanced by "cytoplasmic bridges" coming even to real "cellular fusions". Later, macrophages are also involved in these phenomena. These observations seem to confirm the hypothesis that the lymphocyte surface performs significant functions in detecting its surroundings and clearly underlines this cell capacity of reacting to specific substances such as interferons.

Cell Membrane↗