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G Corradi

Publications and source records attributed to G Corradi.

At least 19 recordsLinked to original sources
PubMed1998

[Current viewpoints on the evaluation of testicular biopsy].

The aim of this study was to analize clinical diagnoses and histopathological reports in 185 cases of male infertility, investigated by testicular biopsies in the period 1993-1997. The classical objective of such investigations has been to clarify either obstructive, or so-called secretory lesions resulting in azoospermia. Histologically normal spermatogenesis was reported in 8 patients (4.3%). Hypospermatogenesis proved to be the most frequent pathologic lesion (69 cases, 37.3%), followed by maturation arrest (61 cases, 33%). Germ cell aplasia (Sertoli-cell-only syndrome) was found in 28 cases (15.1%). Tubular atrophy was the dominant lesion in 5 cases (2.7%). Inflammatory change without other histologic abnormalities was seen only in 1 biopsy (0.5%). Further 13 specimens (7%) showed miscellaneous lesions, including one intratubular germ cell neoplasia. Retrospective histopathologic analysis revealed the coexistence of different basic lesions in 43 of our cases (21.7%). According to the novel requirements, pathologists should always report the presence of germ cell forms in the specimen, which are potentially suitable for fertilization. This is essencial because the new methods of testicular spermium extraction and intracytoplasmatic spermium injection offer the chance of assisted fertilization, even for some patients with smaller testicles and slightly elevated FSH levels.

Biopsy↗
PubMed1998

[Serum and salivary testosterone levels in erectile dysfunction].

In this study 66 male patients with erectile dysfunction were investigated. The authors measured the testosterone levels in serum and in saliva, which latter represent with good accuracy the serum levels of free testosterone. The mean serum total testosterone level was 17.6 nmol/L (confidence intervals: 15.5 and 20.2 nmol/L, normal range: 10-50 nmol/L). The mean salivary free testosterone level was 218.5 pmol/L (confidence intervals: 198.3 and 239.9 pmol/L, normal range: 200-1000 pmol/L). Low salivary (free) testosterone levels were found in 36.4% of patients, while only in 10.6% of patients had low serum testosterone levels (p = 0.01, by binomial test). Although there is a relationship between serum and salivary testosterone levels (r = 0.41, p < 0.001), the patients with low salivary (free) testosterone levels have in major part a normal serum total testosterone level. These data indicate that a considerable proportion of patients with erectile dysfunction have androgen deficiency. The serum total testosterone level is not a sensitive indicator to detection of hypogonadism. The androgen substitution therapy has a beneficial effect on erectile dysfunction in a significant part of patients. The measurement of free testosterone level in saliva may have an important role both in the diagnosis of diseases characterized by androgen deficiency and hyperandrogenic status.

Adult↗
PubMed1996

Interlaboratory reproducibility in reporting inadequate cervical smears--a multicentre multinational study.

A set of 300 vaginal smears was interpreted by 13 cytologists from six European laboratories, who were requested to report inadequate and suboptimal smears. The set had been appropriately seeded to reach approximately 10% inadequate and 20% suboptimal smear frequency. According to the majority report, 230 smears were classified as adequate (76.7%), 43 as inadequate (14.3%), and 27 as suboptimal (9.0%). Agreement with the majority report ranged from 52% to 91% (average 78%). Kappa statistics for reporting inadequate smears showed a high level of agreement for five cytologists, and fair to good agreement for eight. In contrast, kappa statistics for reporting suboptimal smears showed fair to good agreement with the majority report only in five instances, whereas agreement was poor for eight cytologists. 'Inadequate smear' rates may be used to compare the quality of smears received in different laboratories, as there is a high level of agreement among cytologists as to what constitutes an inadequate smear. However, this is not true for "suboptimal smear" rates, and more precise reporting criteria must be defined and tested if an intermediate category is to be retained to report poor quality smears: more precise reporting criteria must be defined and tested if an intermediate category is to be retained to report poor quality smears.

Europe↗
PubMed1996

Detection of Chlamydia trachomatis in the prostate by in-situ hybridization and by transmission electron microscopy.

Chlamydia trachomatis infection has been investigated in the prostate gland using in-situ DNA hybridization and transmission electron microscopy. Sixty-four samples of tissue removed by trans-urethral resection or by open surgery from men with benign prostatic hypertrophy (BPH), were examined histologically and 20 of the cases were found to be positive for mononuclear cell infiltration. Some samples were then investigated using in-situ hybridization and transmission electron microscopy. Out of 20 tissue samples with mononuclear cell infiltration, nine were found to be positive for C. trachomatis infection.

Chlamydia Infections↗
PubMed1995

[Detection of Chlamydia trachomatis in chronic prostatitis by in situ hybridization (preliminary methodical report)].

(Preliminary methodical report). In situ hybridization of Chlamydia trachomatis in formalin fixed, paraffin embedded specimens from the Urology Clinic were performed in 2nd Department of Pathology Semmelweis University of Medicine by a biotin labelled DNA probe. Chlamydia trachomatis is suspected to be responsible for the chronic abacterial inflammation of the prostate besides Ureaplasma urealyticum and Mycoplasmae. According to our retrospective study out of 79 biopsy specimens 34 had the diagnosis of chronic abacterial prostatitis. We examined 11 specimens of them. Bacteria were not identified. Three specimens were positive for Chlamydia trachomatis which were improved by transmission electron microscope. The age of patients was between 59 and 81 years. The detection of chlamydia infection rises the suspicion of a "healthy" career and especially at younger age could cause Chlamydia trachomatis associated genital disease. The undiagnosed and untreated diseases may lead to infertility.

Adult↗
PubMed1995

Is seminal fluid a suitable specimen for detecting chlamydial infection in men?

As genital chlamydia infection is considered the most prevalent sexually transmitted bacterial disease worldwide, its correct diagnosis has a great clinical and epidemiological importance. Urethra is the recommended site for collecting specimen for detection of Chlamydia trachomatis in men, but urethral swabbing is very inconvenient for the patients. Testing of seminal fluid has two advantages: taking the sample is more convenient for the patient, and the ejaculate would provide more information on the possible infection of the upper genital tract. Three non-culture methods-enzyme immunoassay (EIA), DNA hybridization, and polymerase chain reaction (PCR)-were used for evaluation of suitability of seminal fluid as a sample for detection of Chlamydia in 4 patient groups comprising 259 symptomatic and asymptomatic, urology and andrology patients. The seminal fluids were tested parallel with the urethral samples. In one group of patients with typical prostatic complaints expressed prostatic secretion was examined parallel with urethral swab. C. trachomatis positivity rates were much higher in symptomatic (49.1%) than in asymptomatic patients (12.3%) in all kinds of sample. Seminal fluid was somewhat more frequently positive (7.9%) than urethral sample (6.2%). Simultaneous positivity was relatively rare (3.3%), it occurred most frequently in samples of patients with possible accessory gland infection tested by EIA. In a group of 17 patients with typical prostatic complaints the expressed prostatic secretion alone was positive in 7 cases, whereas only other two of the parallel urethral samples proved positive by PCR, simultaneous positivity occurred in one case. These findings suggest that testing of the ejaculate and/or the prostatic secretion parallel with the urethral sample would improve the sensitivity of detection of chlamydial infections.

Chlamydia Infections↗
PubMed1994

[Effect of pyospermia on fertility. Studies by selective leukocyte staining, elastase determination and routine sperm parameters].

The effect of cellular immune response on male fertility was investigated. 92 patients--attending the out patient practice of our Andrological department and 15 patients undergoing vasectomy for sterilisation purpose--were included in this study. For measuring the cellular immune response granulocyte concentration and PMN-elastase level were investigated in ejaculate, former according to WHO manual, and PMN-elastase concentration by ELISA technic. Elastase is a very sensitive non organ specific marker of inflammation. Pathological granulocyte concentration was present in 10.8%, slightly elevated level in 4.3% of cases, totally in 15.1%. Pathological elastase level was found in 17.4%, slightly elevated in 16.3%, totally in 33.7% of cases. High level of correlation was present between the two parameters of inflammation r = 0.695. The incidence of the two parameters in fertile and subfertile patients groups was not significant. Conventional spermaparameters (cell concentration/ml, motility %, intensive motility %, morphology %) were significantly different in the leucospermic and non leucospermic patient groups, except cell concentration. (P = 0.06, P = 0.04, P = 0.03, P = 0.001). The incidence of high elastase level was very similar P = 0.07, P = 0.005, P = 0.03, P = 0.005. As conclusion of our investigation we conclude that cellular response could have a negative influence on male fertility as "side-effect" of the cytotoxic influences of immune defense.

Adult↗
PubMed1993

[Genital chlamydia infection in andrologic patients. Direct antigen detection and serologic screening].

Authors investigated the frequency of genital chlamydial infection. 83 couples--complaining with infertility--were examined. Twenty patients were chlamydia positive by direct IF antigen detection. All chlamydia positive patients have elevated IgG antibody levels in sera. In 33 cases (18.1%) the antibody titers were significantly high above 1:128, in 58 cases (34.9%) antibody levels were slightly elevated between 1:16-1:64. Authors are analysing the questions of the humoral immune reactions and the results of the medical treatment.

Adult↗
PubMed1992

[Andrologic significance of genital mycoplasma].

The authors investigated the effect of genital mycoplasma infection of fertility. The rate of silent mycoplasma infection was studied in asymptomatic andrological patients (655 persons) and in a control group of so called "chronic prostatitis" (1085) patients with clinical symptoms. In the asymptomatic andrological group the urethral smear was positive in 12.5% of cases, the ejaculate in 22.0% of cases. In the "chronic prostatitic" group mycoplasma infection was present in urethral smear in 34.8% of cases, and in the ejaculate in 37.0% of cases. The isolation showed that 29.2% of positive ejaculate samples contained M. hominis, and 70.8% of samples was infected by U. urealyticum. For investigating the effect of mycoplasma infection on the male fertilization capacity, infected and non infected andrological patients was compared by conventional andrological parameters and by modern functional tests like bovin mucus penetration test, hypoosmotic swelling test and swim up procedure. All the modern parameters and one of the conventional tests (sperm motility) showed a significant difference between the two groups.

Chronic Disease↗
PubMed1992

[Significant bacteriospermia. Value and limits of sperm count in andrology].

The data concerning bacteriospermia--are very different in the literature. Asymptomatic andrologic patients, and as control group--patients of the outpatient department for male adnexitis--were studied. Cultivation of aerobic bacteria and mycoplasmas has been recently accomplished by quantitative bacteriological analysis and chlamydia diagnostic. The microbiological screening of 210 andrologic patients ejaculate has given the following results: 19.6% were sterile, 21.0% were colonised by apathogens, 59.1% were positive: 6.2% Mycoplasma hominis, 15.8% Ureaplasma urealyticum, 13.3% Chlamydia trachomatis, 51.9% other aerobic pathogens. 27.6% of samples contained more then one kind of bacteria strains. Quantitative isolation of aerobics and mycoplasmas in both andrological and male adnexitis patients groups showed the following distribution. In the andrologic groups 14.7% of samples contained 10(4) CFU/ml, 9.6% more than 10(4) CFU/ml aerobic bacteria; in the male adnexitis group nearly twice higher values could be observed (20.2% and 19.5%). The distribution of mycoplasma CFU/ml was the following; andrologie group: 20.1% of samples contained 10(4) mycoplasma CFU/ml, 37.5% more than 10(4) CFU/ml; in the male adnixitis group the identical values: 22.9%, 66.9%. Chi 2 statistical analysis showed significant difference ((p = 0.000) in the distribution of data in the two groups. On the other hand both of the groups contained all kinds of bacteriological concentration and even negative cases. Therefore authors suggest more biochemical investigations for detecting inflammatory diseases. Spermaparameters (motility and progressive motility) of andrological patients, divided by sperm concentration (cell number above and under 20 million/ml) and the degree of infection (non infected, slightly infected, and above 10(4) CFU/ml) were significantly different in the seriously infected group by variance analysis (p = 0.000).

Bacterial Infections↗
PubMed1992

Morphologic analysis of serous effusion cells by scanning electron microscopy.

The authors used scanning electron microscopy (SEM) to examine the cells obtained from serous effusions of patients affected with a neoplasm or benign disease. Cellular morphology was studied in non-neoplastic effusions to distinguish benign cells from cancer cells with which they are almost always suspended in malignant effusions. Tumor cells differ in some features such as shape, size, arrangement and especially in surface structures (microvilli), the latter being very noticeable when using this method. The investigation enabled us to note several changes in the cell surface that could reflect particular activities of the cells themselves. For this reason we believe that the technique may be considered very useful to gain knowledge of cellular morphology. However, it cannot be considered suitable for making routine diagnostic conclusions.

Adult↗
PubMed1992

Performance of the mucinous-like carcinoma-associated antigen in serous effusions.

The mucinous-like carcinoma-associated antigen (MCA), a new tumour marker, was dosed in 150 serous effusions. The levels of MCA were measured by means of an enzyme immunoassay (EIA). Ninety-seven were pleural effusions, 49 were peritoneal effusions and 4 were pericardial effusions. Thirty-five effusions were caused by breast carcinoma, 19 by ovarian carcinoma, 17 by pulmonary carcinoma, 19 by various cancers, while 60 effusions turned out to be of non-neoplastic origin. The results were considered in comparison with the cytologic examination, and the data obtained were also elaborated in terms of sensitivity, specificity, predictive value and test efficiency. The EIA showed satisfactory statistical parameters principally regarding ovarian metastatic cancer. On the other hand, less satisfactory results were extrapolated on breast and lung cancers. Even if further studies are necessary, for example to compare MCA with other seric tumour markers, this experience suggests that MCA could be a satisfactory tumour marker in the diagnosis of metastatic ovarian effusions.

Antibodies, Monoclonal↗
PubMed1991

[New function tests in andrologic practice].

The authors compared the classical sperm parameters (cell count, motility, morphology) to new functional tests, such as swim up, hypoosmotic swelling test and in vitro fertilization. The results of hypoosmotic swelling test showed a very good correlation with the fertilization in vitro (P = 0.0001). The authors suggest to make the new functional probes together with the classical examination in the routine andrological practice.

Female↗
PubMed1991

Experience with the Peponen capsule in the management of benign prostatic hyperplasia.

Sixty patients in Stages I and II of benign prostatic hyperplasia were treated with Peponen capsule. Out of them 26 took the drug for 10 months, 22 for at least 7, and 12 for at least 4 months. The daily dosage was 3 x 2 capsules in the first month and 3 x 1 capsule for the rest of the time. On the ground of urodynamic test results and changes in subjective complaints, more than 80% of the patients experienced improvement. The therapy intensified the uroflow, appeased dysuria, the difficult and painful discharge, and reduced the frequency of nocturnal urination.

Humans↗
PubMed1990

Use of monoclonal antibodies to human breast-tumor-associated antigens in the diagnosis of fine-needle aspirates of breast nodules: results of a multicenter study.

Fine-needle aspiration (FNA) cytology is being increasingly employed in conjunction with physical examination and mammography in the pre-surgical diagnosis of breast nodules. In the present study, we have submitted to multicenter validation an immunocytochemical test which employs monoclonal antibodies (MAbs) to breast-tumor-associated antigens (BTAA) for the diagnosis of breast cancer. The results of this analysis, which has evaluated 846 FNAs, show that the immunological test has a sensitivity of 88.62%, a specificity of 97.9% and an accuracy of 92.4%. The predictive value of a positive and a negative finding were 98.4% and 85.57% respectively. Comparison between the cytological and immunocytochemical diagnosis displayed a higher sensitivity, accuracy and predictive value of a negative result with the latter method (p less than 0.01). Our findings clearly indicate that immunocytochemical methods can complement and improve the diagnostic accuracy of FNA cytology of breast nodules.

Antibodies, Monoclonal↗
PubMed1990

The possibility of applying a monoclonal tumor-restricted antibody in cytologic diagnosis of lung cancer in serous effusions.

Fifty serous effusions resulting from a lung disease have been examined. A tumor-restricted anticarcinoma of the lung (not a small cell lung carcinoma) MAb, termed HuTu-m3, has been used together with conventional cytology to examine the cells. The results achieved by immunocytochemical examination have been compared with cytologic results and the data obtained have been evaluated in terms of sensitivity, specificity, predictive value and test efficiency. The final analysis suggests that this technique might be helpful in the diagnosis of serous effusions by increasing the sensitivity of cancer cell cytologic detection.

Adult↗
PubMed1990

Immunocytochemical determination of epidermal growth factor receptor with monoclonal EGFR1 antibody in primary breast cancer patients.

Epidermal growth factor (EGF) has been shown to be important in regulating the growth of breast cancer cells in vivo because of its mitogenic action on some breast cancer cell lines in vitro. Immunocytochemical analysis of EGF receptor (EGFr) was carried out on frozen sections in 134 primary breast cancer patients. Overall 68 of 134 (51%) of the tumors were EGFr positive. There was no correlation between EGFr positivity and menopausal status. Regarding the histopathological features, no significant correlations were observed between EGFr expression and tumor size, grading and lymph nodes status. Estrogen (ER) and progesterone (PgR) receptors were detected by an immunocytochemical assay and an equal distribution of EGFr was found regarding steroid hormonal receptors expression. Finally, there was only a positive trend between the proliferative activity of the tumors, as measured by Ki-67 antibody, and the amount of EGFr. Our results suggest the presence of a subclass of breast tumors, characterized by the absence of ER and/or PgR and the presence of EGFr, whose growth appears to be mediated by autocrine growth factors rather than by steroid hormones. The overall picture is that of an independent relationship between EGFr expression and the known prognostic factors in breast cancer.

Adult↗