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Biomedical subjects

G Berge

Publications and source records attributed to G Berge.

At least 37 records · Page 2Linked to original sources
PubMed1984

Degradation kinetics in aqueous solution of cefotaxime sodium, a third-generation cephalosporin.

The degradation kinetics of a 3- acetoxymethylcephalosporin , cefotaxime sodium salt, in aqueous solution investigated by HPLC under different conditions (pH, ionic strength, temperature) and using different buffers. The scheme of degradation involves a cleavage of the beta-lactam nucleus and the deacetylation of the side chain. In highly acidic medium, the deacetylated derivative is easily converted to the lactone. The degradation rate constants were calculated at three pH values (1.9, 4.0, and 9.0) by measuring the residual cephalosporin and the main decomposition products. The degradation pathway is both supported by the results of a primary salt effect and by the agreement between the theoretical pH-rate profile and the experimental values. In the pH range from 3.0 to 7.0, the main process is a slow water-catalyzed or spontaneous cleavage of the beta-lactam nucleus with intramolecular participation of the side chain amido fraction in the 7-position. In alkaline or strongly acidic medium, the hydrolysis is a base- or acid-catalyzed reaction. Of the buffer systems investigated, carbonate buffer (pH 8.5) and borate buffers (pH 9.5 and 10.0) are found to increase the degradation rates, while acetate buffer decreases the degradation rates. The apparent activation energies determined at different pH values are compatible with a solvolysis mechanism and similar to those previously given in the literature for other cephalosporins. Cefotaxime in aqueous solution is slightly less stable than the main cephalosporin derivatives, despite its high resistance to the beta-lactamases and its remarkable biological activity.

Buffers↗
PubMed1977

[The value of possible skull-cap replacement surgery].

After describing the valence of the various possible plastic procedures for covering defects of the skull cap, the results obtained by the authors are presented. In childhood, autografting from the inner layer of the wing of the ilium or from the ribs is given preference. In adults, the authors carry out alloplasty with hot-polymerizing methacrylate in order to prevent a second surgical intervention for obtaining autogenous material. If surgery has to be carried out in the vicinity of the paranasal sinuses, autogenous cartilaginous cubes must be inserted between the sinuses and the alloplasty because direct contact of the alloplastic material with the sinuses exposed to infection hazards must be avoided.

Adult↗