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Biomedical subjects

G Beckman

Publications and source records attributed to G Beckman.

At least 73 records · Page 4Linked to original sources
PubMed1979

Subtypes of transferrin C.

Subtypes of transferrin C were studied by means of isoelectric focusing after complete desialylation of transferrin. Family data were consistent with an autosomal co-dominant mode of inheritance. Studies of serum samples from 75 individuals heterozygous for C and another (B or D) variant showed that the genes (C1 and C2) controlling the C subtypes are allelic to the B and D genes. The C2 gene frequency in Swedes and Swedish Lapps was similar to that found previously in Danes and Germans.

Gene Frequency↗
PubMed1978

No association of red cell adenylate kinase phenotypes with affective disorders.

Red cell adenylate kinase (AK) phenotypes were studies in 195 patients with affective disorders (41 with the bipolar and 81 with the unipolar form of the disease) and 418 controls. No significant differences were found between patients and controls and between patients with different types of affective disorders. Thus the previous observation by Rundle et al. (1977) showing an increased frequency of the AK2 allele in the unipolar group was not confirmed.

Adenylate Kinase↗
PubMed1978

Genetic markers in cycloid psychosis.

Frequencies of HLA antigens, blood groups, serum groups and red cell enzyme types in patients with cycloid psychosis were compared with those in patients with bipolar psychosis and in normal controls. Patients with cycloid psychosis showed (1) an increased frequency of the Rh-negative type compared to controls, (2) an increased frequency of the K(+) phenotype compared to both bipolar patients and controls, and (3) an increased frequency of the serum group Gc2--1.

Bipolar Disorder↗
PubMed1978

Serum protein and red cell enzyme polymorphisms in affective disorders.

Frequencies of serum groups (Hp and Gc) and red cell enzyme types (PGM1, 6-PGD and ES D) were studied in 195 patients with affective disorders. The patients were classified into four groups: (1) bipolar (manic-depressive) psychosis; (2) unipolar, recurrent, depressive psychosis; (3) non-psychotic reactive depression, and (4) unclassifiable. The Hp2 gene was increased in reactive and unclassifiable patients, the PGM1 1 gene was increased in bipolar patients and the ES D1 gene in reactive patients. No associations were found between affective disorders and the Gc and 6-PGD systems.

Affective Symptoms↗
PubMed1978

Acid- and alkaline phosphatase in amniotic fluid in normal and complicated pregnancy.

171 samples of amniotic fluid were obtained by abdominal amniocentesis from 67 women with complicated pregnancies (isoimmunization, diabetes mellitus or toxaemia). The levels of heat-labile alkaline phosphatase (HLAP), heat-stable alkaline phosphatase (HSAP) and acid phosphatase (AcP) were determined and compared to the enzyme levels in 179 samples from women with normal pregnancies of corresponding gestational ages. HLAP showed two "peaks" of activity, one in the 5th-22nd week and the other at term. HSAP and AcP showed increased activity at term. HSAP was decreased (p less than 0.01) in isoimmunization between the 36th and 40th week. 11 cases of toxaemia with placental insufficiency showed no differences in the levels of HLAP and HSAP compared with normal pregnancy. AcP showed no differences between normal and complicated pregnancy. Samples contaminated by blood showed no significant increase in the acid- and alkaline phosphatase levels. Samples contaminated by meconium showed a complex pattern. Some samples had normal enzyme levels, some had high levels of HLAP only and some had high levels of HSAP and AcP. The origin of the enzymes is not known with certainty. HSAP in amniotic fluid is most likely not of placental but intestinal origin. Determinations of acid- and alkaline phosphatase in amniotic fluid seem to be of little values in the clinical management of complicated pregnancy.

Acid Phosphatase↗
PubMed1977

Purification and partial characterization of the I variant of placental alkaline phosphatase.

The I variant of placental alkaline phosphatase was purified to homogeneity by means of DEAE-cellulose chromatography, isoelectric focusing, and gel filtration on AcA-34. The specific activity of the I variant was found to be 3.33 micronkat/mg. The enzyme is a dimer with an isoelectric point of 4.6 and a molecular weight of 120,000 as determined by sodium dodecylsulfate electrophoresis. The amino acid composition and other physiocochemical properties of the I variant were compared with those of the more common F and S variants. The low activity associated with the I variant is apparently not due to a low specific activity, but to decreased molecular stability. The behavior in the ultracentrifuge and other observations suggest that the I variant differs from the F and S variants in surface charge distribution.

Alkaline Phosphatase↗
PubMed1977

Different composition of the eosinophilic bone marrow pool in reactive eosinophilia and eosinophilic leukaemia.

The composition of the eosinophilic cell series in the bone marrow has been analysed in 10 patients with a pronounced reactive eosinophilia (RE) and in 2 with eosinophilic leukaemia (EL). An imparied differentiation of the eosinophils was found in the EL patients compared with the RE group. Thus the ratio of eosinophilic promyelocytestmyelocytes: segmented eosinophils was 9.2 and 9.1, respectively, in the patients with EL and 0.1--3.1 (average 1.3) in the RE patients. It is suggested that EL is characterized by an impaired differentiation of the eosinophilic bone marrow cells and that the recognition of this abnormality is of value in the diffential diagnosis between EL and RE.

Adult↗
PubMed1977

Superoxide dismutase in Bacteroides fragilis and related Bacteroides species.

Superoxide dismutase (SOD) activity was demonstrated in cell-free extracts of Bacteroides fragilis, Bacteroides vulgatus, Bacteroides distasonis, Bacteroides ovatus, and Bacteroides thetaiotaomicron. The strains were grown under anaerobic conditions in Trypticase soy broth, and the specific activity of SOD in the extracts was, in most strains, higher than in cell-free extracts of Escherichia coli B grown under anaerobic conditions. Isoelectric focusing of the extracts in polyacrylamide gel demonstrated distinct forms of SOD in the different species.

Anaerobiosis↗
PubMed1977

Population studies in northern Sweden. VIII. Ethnic heterogeneity and prenatal selection in the esterase D polymorphism.

The esterase D polymorphism was studied in samples of red cells from different populations and in extracts of chorionic tissue from abortuses and placentae at term. No frequency differences were found between Finns, Lapps, and Swedes. The high frequency of the ES D2 gene previously observed among Lapps was not confirmed in this study. The distribution of ES D phenotypes was very similar in spontaneously aborted fetuses, newborns and adults, hence there was no evidence for prenatal selection operating in this polymorphism.

Adult↗
PubMed1977

Red cell enzyme types in psoriasis.

Seven red cell enzyme systems were examined for electrophoretic variation in a series of psoriatic patients. In two polymorphic (ADA, ES D) and four non-polymorphic systems (SOD, MDH, LDH, Dia) no deviations from normal controls were found. In the acid phosphatase system the frequency of the pa gene was found to be decreased.

Acid Phosphatase↗
PubMed1977

Chromosome aberrations in workers exposed to arsenic.

The occurrence of chromosome aberrations was studied in short-term cultured lymphocytes from nine workers exposed to arsenic at the Rönnskär smeltery in northern Sweden. In the smelter workers, 87 aberrations were found in 819 mitoses. The number of aberrations varied individually from 0 to 25 aberrations per 100 cells. In a control material 13 aberrations were found in 1012 mitoses. The frequency of chromosome aberrations was significantly increased among the smelter workers, but due to the simultaneous exposure to other agents the effect of arsenic per se can not be assessed with certainty.

Arsenic Poisoning↗
PubMed1976

A comparison between the common type and a rare genetic variant of human cupro-zinc superoxide dismutase.

Human cupro-zinc superoxide dismutase is polymorphic in northern Sweden. The genetic variant type has a lower mobility at electrophoresis in alkaline buffer. The enzyme was isolated from erythrocytes from one of the rare homozygotes and its properties compared to those of the common type. The isoelectric point of the variant was higher (4.85) than that of the common type (4.7). Small differences in amino acid composition were found but no definite amino acid substitutions could be pointed out. The molecular weights were equal as judged from electrophoreses in polyacrylamide gels in the presence of dodecylsulphate. The ultraviolet spectra were similar. Parameters related to the active site of the enzyme were very similar; i.e. specific activity and sensitivity to inhibition by cyanide and by H2O2. These parameters, especially the latter two, differ widely between species. Both enzymes were stable for weeks at neutral pH at 37 degrees C, whereas the common type was significantly more stable at pH 4 and pH 11 and also at incubation in neutral buffer at 70 degrees C. It appears that the active site of the variant is conserved whereas the stability of the enzyme is affected.

Amino Acids↗
PubMed1976

Serum naphythylamidase isoenzymes during hormonal treatment. Electrophoretic and quantitative studies.

Alterations in serum naphthylamidase isoenzymes were studied by electrophoretic and quantitative methods in women treated with oral contraceptives and women treated with naturally occurring conjugated estrogens for climacteric symptoms. In women treated with oral contraceptives the appearance of extra isoenzyme components was accompanied by a distinct and significant increase in the total serum naphthylamidase activity, whereas in treatment with conjugated estrogens no such increase was found. The result suggests that combined oral contraceptives and natural estrogens affect the serum naphthylamidase pattern in different ways.

Adult↗