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Biomedical subjects

G Bauer

Publications and source records attributed to G Bauer.

At least 469 records · Page 26Linked to original sources
PubMed1982

Epstein-barr virus induction by a serum factor: IV. Ubiquitous occurrence of the factor within vertebrates and its interaction with defined lymphoid cell lines.

We have recently reported the existence of a serum factor that induces Epstein-Barr virus antigens in Raji cells (Bauer et al., 1982b, c). Here we demonstrate the ubiquitous presence of this factor in sera of vertebrates of all five classes. Furthermore, we tested 48 EBV-genome-positive lymphoid cell lines of different origins for responsiveness to the serum factor. Only cells from lines of two groups, i.e. Burkitt's lymphoma and EBV-transformed marmoset lines, responded to the factor, whereas human lymphoblastoid cell lines were not induced by the serum factor.

Animals↗
PubMed1982

Epstein-Barr virus induction by a serum factor. Purification of a high molecular weight protein that is responsible for induction.

Serum contains a factor that induces Epstein-Barr virus antigens in latently infected human lymphoblastoid cell lines and that cooperates with chemical inducers. Here we report the purification of a protein that is responsible for the effect. Using ammonium sulphate precipitation, chromatography on DEAE-agarose and cellulose, molecular sieve chromatography on Bio-Gel A-5, and glycerol gradient centrifugation, the factor was enriched about 300,000-fold compared to calf serum. Under neutral conditions, the protein chromatographed as a high molecular weight protein of about 5.5 X 10(5) both in its active and inactive form. Both forms of the molecule sedimented at about 7 s. The factor is an acidic protein with a pI of 5. It seems to be composed of high molecular and low molecular weight subunits. Nanogram amounts of purified factor are sufficient for measurable inducing effects.

Animals↗
PubMed1982

Epstein-Barr virus induction by a serum factor. Characterization of the purified factor and the mechanism of its activation.

Purified Epstein-Barr virus-inducing factor shows all the properties previously described for crude serum, i.e. inducing activity, cooperative activity with the inducers 12-O-tetradecanoyl-phorbol-13-acetate, 5'-iodo-2'-deoxyuridine, N-butyric acid, anti-IgM, and the need to be activated by pH shock. A small subpopulation of untreated factor molecules is fully active, however. The factor is a protein that is characterized by considerable heat resistance and sensitivity to reducing agents. It does not seem to require sugar residues or lipid for its activity. Activation occurs both at alkaline and acidic pH. Inactive and activated factor differ in their sensitivity to trypsin and in the degree of sensitivity to reducing agents. A change of conformation within the molecule seems to be the biochemical basis for the activation reaction.

Animals↗
PubMed1982

Supratentorial extracerebral cysts of the middle cranial fossa. A report of 23 consecutive cases of the so-called temporal lobe agenesis syndrome.

23 cases of supratentorial extracerebral cysts of the Sylvian fissure in children and adult patients are reported. There were 21 male and 2 female patients and the medial age was 15 years (range 2--60 years). The cysts were located in the left middle cranial fossa in 18 cases, in two cases there were bilateral cysts and three patients only had right-sided lesions. In 14 patients the presence of the cyst was uncomplicated. In two of these patients the cyst was detected only incidentally. Four patients had subdural haematomas in addition to the cyst. Two patients had subdural haematomas and were hydrocephalic as well. Three patients had large cysts and hydrocephalus. We did not encounter intracystic bleeding. Operation on the cysts by uncapping them was done in four cases. In all four instances histological examination of the cyst wall revealed that it was composed of arachnoid. Uncapping of the cyst was not followed by its diminution in any of the four patients. It seems that uncapping of such cysts is not a helpful treatment and that surgical treatment in our cases should have been restricted to complications such as subdural haematomas and hydrocephalus. A search for better operative methods for the treatment of large extracerebral supratentorial cysts of the middle cranial fossa should be undertaken.

Adolescent↗
PubMed1982

[Activities in the alpha band in unresponsive patients (author's transl)].

Activities in the alpha band are to be encountered in unresponsive patients. A review is given over the different conditions. Patients suffering from pontine lesions mostly are not comatose but locked-in. Alpha activities with intoxications and with comatose states due to transtentorial herniation can be distinguished by the accompanying neurological signs. In severe metabolic coma alpha activities are to be observed as an epileptic phenomenon. Monorhythmical alpha activities with hypoxic coma are not accompanied by slow waves, succeed a burst suppression pattern and are an ominous prognostic sign.

Adult↗
PubMed1981

The relationship between tumor promoter binding and Epstein-Barr virus induction in human lymphoblastoid cell lines.

The tumor promoter TPA (12-0-tetradecanoyl-phorbol-13-acetate) efficiently induces the synthesis of Epstein-Barr virus (EBV) antigens in EBV-genome-harboring human lymphoblastoid cells. We attempted to obtain information on the binding of TPA to cells and on the relationship between TPA-binding and EBV induction by the use of tritiated TPA (3H-TPA). Our data show: (1) In the absence of cells TPA can bind to serum completely within 60 minutes. (2) Cells can compete for a proportion of serum-bound TPA. (3) Binding of TPA to cells reaches equilibrium within 60 minutes and is higher at 37 degrees than 0 degrees C. In the absence of serum, the rate of binding is about twice as high as in the presence of serum. (4) Dissociation of TPA from cells also seems to be rapid. When the cells are incubated with cold TPA after prior treatment with 3H-TPA, followed by washing, a much higher rate of release of labeled TPA is observed than in cultures to which fresh medium is added exclusively. Dissociation is higher in the presence of serum than in the absence of it. If radiolabeled cells are analyzed after serial washing, the rate of cell-associated/noncell-associated radioactivity indicates that the proportion of molecules required for EBV induction is dissociated rapidly.

Blood↗
PubMed1981

[Objective suppositions of liability in criminal law (author's transl)].

The conception of causality in criminal law was based, until the middle of our century, on the equivalent theory, which extended to the objective liability as concerns doctrine and jurisdiction, excluding even the guilt as corrective element in some cases. The dispute on the necessity of adequate theory in penal law became useless as the objective conditions of elevated punishing disappeared. By reducing causality to a purely natural scientific conception the doctrine of objective imputation was developed, with the requirements of risk connection, adequacy, and risk elevation. The significance of this development in criminal law is demonstrated by medical examples, especially medical malpractice.

Austria↗