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Biomedical subjects

G Bartsch

Publications and source records attributed to G Bartsch.

At least 289 records · Page 16Linked to original sources
PubMed1984

Do's and don'ts in stereological pathology or possibilities of stereological-biochemical correlation in uropathology.

The problems and the interpretation of combined stereological-biochemical investigations in benign prostatic hyperplasia (BPH) research are discussed. As BPH is characterized by a primary stromal enlargement the role of the smooth muscle cell (SMC) is focussed. For determination of the single cell volume of the SMC several methods are applicable leading to different results. Thus interpretation of these results has to be done with caution. Finally as an example there is a discussion of a correlation of stereological-biochemical dates of human prostatic tissue samples.

Adult↗
PubMed1984

[Comparative histopathologic and sonomorphological prostate studies].

Ten cadaver prostates were divided into 50 sections and corresponding sections were compared, using ultrasound, with respect to their echogenic structure and histomorphology. The results of normal prostatic tissues, benign prostatic hyperplasia and prostatic carcinomas are described and discussed. It was shown that malignant prostatic lesions showed echo-poor sonograms in vitro. This observation is supported by our clinical experience. Possible explanations are discussed.

Adenocarcinoma↗
PubMed1983

Smooth muscle cell of the canine prostate in spontaneous benign hyperplasia, steroid induced hyperplasia and estrogen or tamoxifen treated dogs.

Human benign prostatic hyperplasia is a predominantly stromal hyperplasia with accumulation of connective tissue. The main ultrastructural finding, which may be causal, is an activation of the smooth muscle cells, as seen by an increase of the volume density of the organelles within these cells. The dog is widely used as an animal model for human prostatic hyperplasia in spite of several differences. In this work the smooth muscle cells of the spontaneous and steroid-induced (by treating castrates with dihydrotestosterone and estradiol) prostatic hyperplasia of dogs were analysed by electron microscopical morphometry, and compared to estrogen or tamoxifen (antiestrogen) treated dogs as well as to untreated or castrated control dogs. The results clearly show that the prostatic smooth muscle cells of the dog can be activated by estrogen as well as tamoxifen, which proves the estrogenic side activity of the latter. In marked contrast to that, neither in the spontaneous nor in the steroid-induced prostatic hyperplasia could an activation of the smooth muscle cells be found. This is a most important difference from human benign prostatic hyperplasia, which limits the use of this animal model, and it might even be the explanation of the different reaction of human and canine prostatic hyperplasia to therapeutic hormonal manipulations.

Animals↗
PubMed1983

Exocrine and endocrine testicular function in patients with unilateral testicular disease.

Amongst 30 patients who had undergone untwisting and fixation for unilateral torsion of the testis, seminal analyses were normal in 15, equivocal in 3 and pathological in 12; and of 46 men with cryptorchidism who had had unilateral orchidopexy, seminal analyses were normal in 13, equivocal in 23 and pathological in 10. Follicle-stimulating hormone levels were found to be elevated in those with equivocal and pathological seminal analyses in both groups. Assessment of testicular function before orchidectomy in 36 patients with seminomas and non-seminomatous testicular tumours revealed only one (with a HCG-negative seminoma) with a normal seminal analysis. Men with HCG-positive testicular tumours were found to have decreased follicle-stimulating hormone levels and elevated 17-beta-oestradiol and prolactin levels.

Adolescent↗
PubMed1983

4. Steroid pathophysiology of BPH. Correlative morphological and biochemical investigations on the stromal tissue of the human prostate.

Light and electron microscopic measurements show that tissue overgrowth in human benign prostatic hyperplasia is mostly due to an increase in stromal tissue. There is evidence that interaction between stromal and epithelial cells play a critical role in the regulation of the coordinated growth of the prostatic gland. Stromal response to hormones is virtually not completely understood, however at present there are strong indications that it is the androgens above all which control the function of the stromal part of the human prostate. It has been shown that the endogenous concentration of 17 beta-estradiol in the stromal compartment of the guinea pig prostate exceeded that of the blood plasma and prostatic epithelium. In an attempt to shed light on the action of endogeneous androgens and estrogens on the stromal tissue of the human prostate, selective androgen and estrogen antagonists as well as bromocriptine were used to study the stromal reaction pattern in human prostatic hyperplasia.

Androgen Antagonists↗
PubMed1983

The effect of miconazole on ergosterol-less mutant of Candida albicans.

The effect of miconazole in fungistatic concentration on an ergosterol-competent Candida albicans strain and its ergosterol-nonproducing mutant was investigated by scanning and transmission electron microscopy. The ergosterol-less mutant showed pronounced alterations by scanning electron microscopy. The increased sensitivity to miconazole of the ergosterol-less mutant was attributed to an altered plasma membrane composition and rigidity.

Candida albicans↗
PubMed1982

[Chromosomal findings in patients with oligospermia and azoospermia (author's transl)].

A cytogenetical study has been conducted in patients with impaired spermatogenesis and reduced fertility or complete sterility to investigate the frequency of chromosomal aberrations in this condition. 78 patients with azoospermia and 74 patients with a sperm count of below 20 million/ml have been studied cytogenetically and endocrinologically between 1977 and 1981. Numerical or structural chromosomal aberrations were found in 27 out of the 152 patients. In two phenotypically normal males hitherto-undescribed balanced autosomal translocations were detected.

Chromosome Aberrations↗
PubMed1982

Sonometrics of the prostate.

Determination of prostatic size by rectal examination is inaccurate if objective and reproducible values are desired. Sonometrics, a combination of transrectal sonography and biometrics, is a simple method to determine prostatic weight. The optomanual image analyzing system (BIT PAD-TRS 80) consists of a digitizer tablet, cursor, processing unit and keyboard (TRS 80). The photographic records or the image of the prostate on the scan can be measured with the optomanual image analysis system by tracing the circumference of the prostate. Using the computer one can calculate length, area and circumference of the sonogram. When the postoperative and sonometrically estimated weights were compared a good correlation coefficient was found. The studies on age-dependent growth of the prostate show an age-volume relationship similar to that shown in the study by Swyer. As demonstrated in patients with hypothalamic hypogonadotropic hypogonadism change in prostatic volume also can be assessed in small prostates.

Aged↗
PubMed1982

Spermiometrics: objective and reproducible methods for evaluating sperm morphology.

An approach was made to replace the subjective evaluation of human sperm morphology by an objective method applicable in routine laboratories. 20 patients out of infertile marriages (G2 and G3) were compared with 10 normal, fertile men (G1). Two different methods were used. Highly developed instruments and techniques were used in method 1, and cheap and simple ones, designed for routine laboratories, in method 2. Head area, circumference, length, and maximal midpiece width of 100 spermatozoa/subject were determined by a semi-automatic image analysis system. Head variation and average midpiece width were clearly bigger in G2 and G3 than in G1. As a result, formulae were developed, determining the pathological alteration of sperm head and midpiece for the individual semen. In addition, an objective classification into different sperm types was elaborated. Using the developed formulae, results of methods 1 and 2 are well in agreement. Thus, method 2 is recommended for routine laboratories, allowing an objective and reproducible morphology determination within 30 min.

Clinical Laboratory Techniques↗
PubMed1981

Late results after surgical treatment of maldescended testes with special regard to exocrine and endocrine testicular function.

Testicular volumes and exocrine testicular function were determined in 82 men who had undergone orchidopexy (36 bilateral, 46 unilateral) at 5-18 years of age. The testicular volumes in unilateral and bilateral orchdopexied patients correspond to those on normal mature males. Semen analyses in unilateral cryptorchidism were normal in 13, doubtful in 23 and pathological in 10 patients. Out of a total of 36 patients who have undergone bilateral orchidopexy, 3 patients were found to have normal, 7 patients doubtful and 26 patients pathological sperm analyses. Endocrine evaluation in bilateral cryptorchidism (prepuberal, puberal and postpuberal) showed no differences in testosterone levels compared to control groups. Some postpuberal patients with pathological sperm analyses were found to have elevated LH and FSH levels; 22 postpuberal patients with pathological sperm analyses showed hypergonadotropism with markedly elevated LH and FSH levels after GnRH.

Adolescent↗