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Biomedical subjects

G Asboe-Hansen

Publications and source records attributed to G Asboe-Hansen.

At least 73 records · Page 4Linked to original sources
PubMed1976

Cortisol effect on collagen biosynthesis in embryonic explants and in vitro hydroxylation of protocollagen.

The effect of increasing doses of hydrocortisone acetate, hydrocortisone phosphoric acid complex, and hydrocortisone sodium succinate on collagen biosynthesis was assayed in two different systems. I. Explants of chicken embryo tibiae showed decreased biosynthesis of [14C]hydroxyproline and total and glycosylated [14C]hydroxylysine under the influence of high doses of hydrocortisone. With the lower doses of hydrocortisone acetate, no effect was noticed. The total uptake of the precursor amino acids followed patterns similar to those of collagen biosynthesis. II. Hydroxylation of [14C]proline labelled protocollagen by protocollagen proline hydroxylase was inhibited by high doses of hydrocortisone acetate, hydrocortisone phosphoric acid complex, and hydrocortisone sodium succinate. III. A decreased diffusion of collagen to the medium with increasing doses of hydrocortisone acetate, hydrocortisone phosphoric acid, and hydrocortisone sodium succinate was noticed. IV. No further hydroxylation of new-synthesized collagen was obtained under the influence of hydrocortisone phosphoric acid and hydrocortisone sodium succinate, when the undialyzable material was used as a substrate for protocollagen proline hydroxylase.

Animals↗
PubMed1976

Parallel studies on collagen hydroxyproline and hydroxylysine in human skin biopsies.

Studies on hydroxyproline and hydroxylysine, the two amino acids characteristic of collagen and related glycoproteins, were undertaken on biopsies of pathologic and clinically normal human skin. No statistically significant differences between clinically normal skin of mamma, thorax, axilla, femur, hip and sacral area were found. A decreased collagen content was seen in chronic pemphigus (bullous pemphigoid), amyloidosis, scleromyxedema and the edge of a leg ulcer. Determination of both amino acids is considered necessary to characterize alterations of collagen.

Adult↗
PubMed1976

Ultrastructure of localized myxedema.

Five biopsies from three patients with localized myxedema were studied by electron microscopy. The dermis showed an overwhelming accumulation of microfibrils with knobs (acid glycosaminoglycans) and an amorphous material containing glycoprotein. Furthermore, evidence of degradation without new formation of collagen fibrils was found. Considerable numbers of mast cells with various types of granules, fibroblasts with dilated granular endoplasmic reticulum, and macrophages were seen in the mucinous areas.

Adult↗
PubMed1975

A selective stain for mast cells.

A selective stain for mast cells in tissue sections is presented. The procedure is based on the resistance to destaining with absolute ethanol-acetic acid of the complex acid mucopolysaccharide-Toluidine Blue reinforced with ferrioxamine B.

Acetates↗
PubMed1975

Treatment of generalized scleroderma with inhibitors of connective tissue formation.

103 patients suffering from generalized scleroderma were studied in order to assess the effect of treatment with inhibitors of connective tissue formation. 93 patients with generalized scleroderma were given D-penicillamine, benzyl-penicillin-diethylamino-ethyl-ester hydroiodide, adrenal glucocorticoids, dextro-thyroxine, hydralazine, and "mixed treatment" (one or several of the drugs in consecutive courses, or concurrently). The effect of dextrothyroxine could not be evaluated in this study. No improvement could be seen after adrenocortical steroid therapy. Hydralazine seemed to be effective. D-penicillamine improved 25 of 34 treated patients; penicillin hydroiodide 12 out of 16. The dermal sclerosis of 6 patients regressed completely; in 16, sclerosis regressed with the exception of finger sclerosis; in 32, partial regression was registered; 20 had their progression arrested, but there was no regression; in 19 cases, there was no effect whatsoever. The prognosis seemed to be better for young than for old people. The age at onset was lower in the better groups. The higher the total dose, the better the results. The length of the treatment course is probably of some significance. The short-lasting cases had better prospects than the longer lasting. Ten untreated patients of this material and 11 patients seen earlier showed continued progression. Side effects leading to discontinuation of the drugs were seen in a substantial number of patients, especially after D-penicillamine. Twelve deaths could not be related to the treatments.

Adult↗