Search PubMed⌕ Search

Biomedical subjects

G Asboe-Hansen

Publications and source records attributed to G Asboe-Hansen.

At least 55 records · Page 3Linked to original sources
PubMed1978

Fibrodysplasia ossificans progressiva. Biochemical changes in blood serum, urine, skin, bone, and ectopic ossification.

Increased urinary output of total hydroxyproline, hydroxylysine and uronic acid was found in two patients suffering from fibrodysplasia ossificans progressiva. Before treatment of one of the patients, the high molecular weight peptide fraction deriving from newly synthesised collagen was particularly increased. Treatment with disodium etidronate (diphosphonate) reduced the values. The urinary values of sodium, potassium and calcium were also depressed during treatment, and, in both patients, serum phosphate was high, while serum calcium was normal. The hydroxyproline and hydroxylysine contents in skin and bone of FOP patients did not differ from controls, while ectopic ossifications showed a considerable increase in hydroxylysine in relation to normal bone.

Adolescent↗
PubMed1978

Effects of some connective-tissue active drugs on protocollagen proline hydroxylase activity.

The effect on protocollagen proline hydroxylase of drugs reported to be capable of inducing the lupus erythematosus syndrome and in some way acting on connective tissue and inflammation was studied in an in vitro system for the hydroxylation of 14C-Pro-labelled protocollagen. Some derivatives were also studied. It was found that benzoic acid and phenothiazine derivatives, hydroxyphenols, (+)catechin, beta-amino propionitrile, certain B vitamins, dihydrazinophthalazine, cysteine and dimethylcysteine were inhibitors of PPH. The chelation of Fe2+ions by the compounds mentioned is suggested to be essential.

Animals↗
PubMed1978

Effect of (+)catechin on connective tissue.

The effect of (+)catechin on connective tissue was studied in organ cultures of chick embryo tibiae and after in vivo injection in rats and mice. Collagen biosynthesis and hydroxylation of 14C-Pro-labelled protocollagen in vitro by protocollagen proline hydroxylase (PPH) were inhibited. In vivo, a slight decrease in the biosynthesis of collagen and in the PPH activity in skin of rats and mice was noticed as an effect of (+)catechin. No effect of the drug was observed on the incorporation of 14C-D-glucosamine by chick embryo tibiae. Increased urinary excretion of acid mucopolysaccharide metabolites by the mice injected with (+)catechin was observed.

Aminopropionitrile↗
PubMed1978

Abnormal skin collagen in scleroderma.

A significant decrease in the content of hydroxyproline and hydroxylysine was found in the skin of patients with generalized scleroderma (acrosclerosis), the lowering of Hyp being more marked than that of Hyl. The production of an abnormal collagen or a change from one collagen type to another is suggested to take place.

Collagen↗
PubMed1978

Hydroxyproline to hydroxylysine molar ratio indicates collagen type.

By using the molar ratio Hyp to Hyl, types I and II of collagen can be differentiated in 0.5 to 10 mg of dried, defatted tissue. Analyses on human skin, tendon, bone, aorta, cartilage, as well as nucleus pulposus, and annulus fibrosus of intervertebral discs are reported. Analyses of collagen of mesenchymal tissues of other vertebrates are also reported. From amino acid analysis of purified collagen samples published in the literature, the molar ratio Hyp/Hyl was calculated. Type I and type III collagen were differentiated from type II, and the latter was differentiated from type IV collagen. The molar ratios obtained with our analyses followed closely the values from previously reported amino acid analyses on purified collagen.

Amino Acids↗
PubMed1977

Fibrodysplasia ossificants progressiva.

Two cases of fibrodysplasia ossificans progressiva are reported. Both patients were females and had suffered from the disease since birth. The characteristic anomalies of great toes and thumbs associated with multiple ectopic ossifications of the connective tissue are described. Fasciae, tendons, ligaments, and joint capsules of the proximal parts of the extremities and the dorsal aspect of the trunk were involved. The muscle atrophy was probably a secondary phenomenon.

Adult↗
PubMed1977

Ultrastructure of dermal connective tissue in fibrodysplasia ossificians progressiva.

The ultrastructure of dermal connective tissue in two patients suffering from fibrodysplasia ossificans progressiva (syn myositis o.p.) was studied. The disorder starts with soft tissue swelling which subsequently ossify. The connective tissue of various organs may be affected. No defects in calcium metabolism have been discovered. It was intended to study the primary event which initiates the ossification. The present ultrastructural study demonstrates an accumulation of proteoglycan microfibrils as well as glycoprotein material in dermal connective tissue. Both substances are prerequisites of calcification resulting from the binding of calcium and phosphorus ions. A subsequent release of proteolytic enzymes in the tissues may be set free the bound iones and, thus, initiate mineralization.

Calcium↗
PubMed1976

Micromethod for fractionation of acid mucopolysaccharides.

A new micromethod for fractionation of acid mucopolysaccharides based upon the use of different concentrations of HC1 to separate the complex of CPC with non-sulphated, monosulphated and polysulphated acid mucopolysaccharides (glycosaminoglycans) is presented. The method utilizes the different binding of the anionic macromolecules to the cationic compound cetyl pyridinium chloride. The method is simple and reproducible. The use of HC1 as eluent allows the exclusion of some steps required when salts are used for elution. Hexuronic acids are determined on the eluents.

Chondroitinases and Chondroitin Lyases↗
PubMed1976

Simplified method for determination of protocollagen proline hydroxylase.

Preparation of (14C) Pro labeled protocollagen and requirements for its hydroxylation by PPH was investigated. Protocollagen can be prepared by centrifugation of the biological material at 15.000 X g. Substances in current use, viz catalase, bovine serum albumin and dithiothreitol were found unnecessary under the hydroxylation conditions used. A decrease in PPH in the testis and skin of rats with increasing age was demonstrated.

Animals↗
PubMed1976

An assay for total hexosamine and a differential assay for glucosamine and galactosamine.

Two new procedures are presented for quantitative determination of glucosamine and galactosamine. One, which is proposed for total hexosamine, yields chromogens of equal intensity with equal concentration of glucosamine and galactosamine. There is addition of the correspondent chromogens when they are present in mixtures. The procedure is presented as a manual as well as an automated assay. The other procedure is a differential assay which allows the detection of galactosamine without interference by glucosamine. By the two procedures, the hexosamines present in acid mucopolysaccharides and/or glycoproteins can be determined.

Acetylgalactosamine↗
PubMed1976

Lysolecithin fusion of cells from multiple sclerosis patients with Vero cells.

Investigations were performed on cell cultures derived from patients with multiple sclerosis (MS) in order to trace a possible virus infection as a cause of the disease. Cell cultures were established from one brain autopsy specimen and four lymph node biopsies from MS patients. Lymphocytes from 28 MS patients and six healthy controls were used for fusion or cocultivation experiments, either immediately after isolation or after mixed lymphocyte cultures (MLC). Lysolecithin fusion and cocultivation experiments were made with Vero cells and with, respectively: cultured brain cells, lymph node cells and lymphocytes from MS patients. Electron microscopical examination revealed intranuclear filamentous structures in 5 per cent of the cells in primary cultures of MS brain and lymph node and in control skin organ cultures. Multinucleated cells were found in six out of 19 cocultures of Vero cells and MS lymphocytes preincubated for 2 days at 37 degrees C. The cultures were tested for the presence of viruses, i.e. measles virus and virus producing hemadsorption with human type O and/or guinea pig erythrocytes and virus against which the MS patients showed serum FA-antibodies. No virus antigen could be demonstrated in the cells.

Adult↗
PubMed1976

Influence of corticosteroids on connective tissue.

All glucocorticosteroids inhibit growth, regeneration and repair of cellular or intercellular components of dermal connective tissues, when penetrated through the skin barrier. The resulting atrophy is a logical manifestation of the action of these compounds.

Administration, Topical↗