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Biomedical subjects

G Andersson

Publications and source records attributed to G Andersson.

At least 289 records · Page 16Linked to original sources
PubMed1984

Molecular map of the human HLA-SB (HLA-DP) region and sequence of an SB alpha (DP alpha) pseudogene.

The human major histocompatibility complex contains the genes for at least three different types of class II antigens, DR, DC and SB (DR, DQ and DP). They are all composed of an alpha and a beta chain. We have cloned a chromosomal region of 70 kb containing the SB (DP) gene family in overlapping cosmid clones. This segment contains two alpha genes and two beta genes, located in the order SB alpha 1, SB beta 1, SB alpha 2 and SB beta 2. The orientation of the alpha genes is reversed compared with that of the beta genes. This organisation suggests that the SB region has arisen by duplication of a chromosomal segment encompassing one alpha and one beta gene. Partial nucleotide sequences of the SB alpha 1 and SB beta 1 exons demonstrate that the genes correspond to SB alpha and beta cDNA clones. Consequently these genes are expressed. In contrast nucleotide sequence determination of the SB alpha 2 gene shows that it is a pseudogene.

Amino Acid Sequence↗
PubMed1984

The distribution of microsomal glutathione transferase among different organelles, different organs, and different organisms.

In the present study we have used both enzyme assay with 1-chloro-2,4-dinitrobenzene as substrate and immunochemical quantitation to examine the distribution of microsomal glutathione transferase in different organelles, in different organs, and in different organisms. This enzyme was found to constitute 3% and 5%, respectively, of the total protein recovered in the microsomal and outer mitochondrial membrane fractions from rat liver. Microsomal glutathione transferase present in other subcellular fractions can be accounted for by contamination by the endoplasmic reticulum. In contrast to the situation with rat liver microsomes the glutathione transferase activities of microsomes from extrahepatic tissues of this same animal could not be activated by treatment with N-ethylmaleimide. Nonetheless, significant albeit low levels of a protein with the same molecular weight and immunochemical properties as the rat liver enzyme could be detected in microsomes from several extrahepatic tissues, notably the intestine, the adrenal, and the testis. Of those mammals for which fresh liver could be obtained, all demonstrated N-ethylmaleimide-activatable glutathione transferase activity in their liver microsomes. On the other hand, representatives for fish, birds, and amphibia did not demonstrate such activatable transferase activity in their liver microsomes. Toad was the only species that had a notable (twofold) sex difference in their level of hepatic microsomal glutathione transferase activity.

Animals↗
PubMed1984

Antithrombin III in patients with acute deep vein thrombosis during heparin treatment (subcutaneous and intravenous) and during and after treatment with oral coumarins.

The antithrombin III (AT-III) concentration was studied in 98 patients with symptomatic acute deep-vein thrombosis. All patients were initially treated with heparin randomly by subcutaneous injections or by continuous infusions. Then the patients were treated with coumarins during one or six months. The AT-III concentration was estimated daily during heparin treatment and repeatedly during the first year. The mean AT-III concentration decreased progressively 25% during 5 days of heparin treatment regardless of whether heparin was given intravenously or subcutaneously. The mean AT-III concentration during coumarin treatment was higher than after coumarin treatment. Eleven patients developed recurrent thromboembolic episodes during the follow-up period. The mean AT-III concentration in these patients was not lower than in the patients without recurrences.

Adult↗
PubMed1984

Demonstration of a cuneate relay in a cortico-olivo-cerebellar pathway in the cat.

Stimulation of the forelimb area of the pericruciate cortex evokes climbing fibre responses in the contralateral anterior lobe of the cerebellum. Application of a local anaesthetic (lidocaine) to the surface of the cuneate nucleus reduced the responses to stimulation of the posterior sigmoid gyrus in the c1 and c3 zones but not in the c2 and d1 zones. Lidocaine produced an effect only when applied to the caudal half of the nucleus. Long-latency responses evoked from the anterior sigmoid gyrus in the c1, c2 and c3 zones were unaffected by lidocaine application.

Animals↗
PubMed1984

Subcellular distribution of rat liver porin.

The subcellular distribution of rat liver porin was investigated using the immunoblotting technique and monospecific antisera against the protein isolated from the outer membrane of rat liver mitochondria. Subfractionation of mitochondria into inner membranes, outer membranes and matrix fractions revealed the presence of porin only in the outer membranes. Porin was also not detected in highly purified subcellular fractions, including plasma membranes, nuclear membranes, Golgi I and Golgi II, microsomes and lysosomes. Thus, liver porin is located exclusively in the outer mitochondrial membrane.

Animals↗
PubMed1984

Purification and characterization of a vanadate-sensitive nucleotide tri- and diphosphatase with acid pH optimum from rat bone.

Rat bone was extracted with KCl and Triton X-100, and a tartrate-resistant acid phosphatase activity was purified by protamine sulfate precipitation, ion-exchange chromatography (CM-cellulose), and gel filtration on Sephadex G-200 according to previously described procedures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining demonstrated a major band with an apparent monomer molecular size of approximately 14,000 Da. The enzyme is active with p-nitrophenylphosphate (p-NPP) but exhibits a 5- to 10-fold higher affinity towards several nucleotides of which ATP and ADP are the most readily hydrolyzed substrates based on kinetic studies. Based on sensitivity towards proteolytic treatment and detergent removal, as well as pH-optimum studies, a single enzyme was found to be responsible for activity towards nucleotide phosphates as well as p-NPP. This nucleotide tri- and diphosphatase constitutes around 15% of the total acid phosphatase activity in rat bone. The activity with ATP as substrate in contrast to that with p-NPP was inhibited in a noncompetitive fashion by MgCl2, sodium metavanadate, and p-chloromercuribenzoate. Enzyme activity with p-NPP and ATP is dependent on the presence of KCl and detergent and is activated by Fe3+ and ascorbate. The reported characteristics of the enzyme suggest that it functions as a unique membrane acid ATPase.

Acid Phosphatase↗
PubMed1984

Mutual inhibition between olivary cell groups projecting to different cerebellar microzones in the cat.

This study was carried out predominantly on the b zone in the lateral vermis of the cerebellar anterior lobe. This zone is divided into sagittally oriented microzones which receive a somatotopically organized climbing fibre input. It was shown that the climbing fibre input to one microzone is inhibited by stimulation of a nerve that projects to an adjacent microzone. The degree of inhibition was related to the proximity of the microzones involved. The latency of the inhibition was short and the duration 70-110 ms. The inhibition of climbing fibre responses occurred in the inferior olive and was presumably due to post-synaptic inhibition of the olivo-cerebellar neurones. The mutual inhibition could be produced by antidromic activation of olivo-cerebellar neurones. An inhibition with similar properties as in the b zone, but weaker, was observed between forelimb and hindlimb inputs to the c1 and c3 zones in the pars intermedia. In the c3 zone, an inhibition between adjacent forelimb microzones also occurred.

Animals↗
PubMed1984

Genomic hybridization with class II transplantation antigen cDNA probes as a complementary technique in tissue typing.

Hybridizations of HLA-DR and DC transplantation antigen beta chain cDNA probes to restriction enzyme digested genomic DNA were examined in regard to their potential in tissue typing. DNA from cells, typed by cellular techniques to be homozygous for the specificities Dw 1 to 8, gives rise to unique fragment patterns for each specificity. Hybridizations to DNA from unrelated individuals serologically typed to be DR identical occasionally reveal genetic differences in the class II antigen region, notably with the DC beta cDNA probe. In contrast, hybridizations to DNA from monozygotic twins displayed completely identical patterns with both types of probes. From these data it seems reasonable to conclude that genomic hybridizations with class II antigen probes will be useful in the definition of new class II antigen loci and alleles, in determination of paternity, in studies of class II antigen linked diseases and as a complementary method in conventional tissue typing.

Alleles↗
PubMed1984

Corporate reported sick leave and its relationship with education, responsibility and blood pressure.

The investigation group included all 1313 employees of SAAB-SCANIA, Linköping, in the age group 50-59 years. In 1975 they were called during their working hours to the company's Physical Training Centre to be assessed. This investigation included measurements such as systolic blood pressure, height, weight and certain anthropometric measurements. Other information was obtained from the personnel records about sex, age, type of employment, educational grade, degree of responsibility, and reported sick leave in 1974 and 1975 for the workers, and between 1970 and 1975 for the salaried employees. The total number of drop-outs was 238, or 18%. Sick leave is mainly a problem of the long-term absence of a minority of employees with an ever-increasing rate of absence. Sick leave among the salaried employees decreases in relation to higher education and increased responsibility. A high level of responsibility and education characterizes a low-risk group, whereas little responsibility and a low level of education denote a high-risk group with regard to blood pressure and sick leave. A disparity--low education and medium responsibility--has earlier been described as denoting a high risk of psychosomatic diseases. In this study they showed a rate of absence lower than the average at the same time as the blood pressure is above the average.

Absenteeism↗
PubMed1984

Growth hormone induction of lactogenic receptors at intracellular sites in male rat liver.

Male and female rat livers were fractionated by density gradient centrifugation into Golgi I (mainly secretory vesicles), Golgi II (mainly cisternal elements), and lysosomes. Estimations of fraction purity and representativity were made by marker enzyme and electron microscopic analyses. The binding of [125I]iodo-human GH ( [125I]iodo-hGH) to different subcellular liver fractions were studied. In Golgi I and II the binding specificity was similar in both sexes and indicated that [125I] iodo-hGH binds to a lactogenic receptor. Scatchard analysis showed a larger number of binding sites in female Golgi I (5600 fmol/mg protein), Golgi II (3400 fmol/mg), and lysosomes (1300 fmol/mg) than in male Golgi I (240 fmol/mg), Golgi II (200 fmol/mg), and lysosomes (230 fmol/mg). The apparent dissociation constant was within a similar range (0.6-0.7 X 10(-9) M) in all fractions. Administration of hGH to male rats by continuous infusion (infusion rate, 5 micrograms/h) resulted, after 5 days of treatment, in an increase in the number of lactogenic binding sites in Golgi I and II to levels similar to the binding in the corresponding female Golgi fractions. When rat GH was given to hypophysectomized male rats (infusion rate, 10 micrograms/h) for 1 week, the binding of [125I]iodo-hGH in lysosomal and Golgi fractions was increased to a female level. The present results suggest that lactogenic receptors are located in the Golgi complex as well as the lysosomal compartment and that these receptors can be induced at these intracellular sites with both a somatotropic-lactogenic hormone (human GH) and a pure somatotropic hormone (rat GH).

Animals↗
PubMed1984

Electrical treatment of non-united fractures.

The semi-invasive technique for electrical stimulation of bone healing developed by Brighton et al. (1977) was used in 23 patients with nonunited fractures of the tibia (14 cases), humerus (4 cases), scaphoid, femur and fibula as well as one failed arthrodesis of the ankle. The fractures were clinically not healed and not operated on within a minimum of 6 months. The mean period from fracture to treatment was 18 months. Electrical stimulation led to solid bone healing in 10 cases. Two deep infections occurred during the treatment. Of 13 cases that did not unite, a great range of motion in the nonunion area was an obvious cause of failure in seven cases. The results in this series cannot compete with those of bone graft surgery for nonunions.

Adult↗
PubMed1984

Experience with elective coniotomy.

One hundred and five coniotomies have been carried out, as an alternative to tracheotomy on patients in the ICU, Danderyd Hospital. The procedure is easy to perform, relatively riskfree and is attractive in terms of resources required. However a rather high frequency of voice-changes were found.

Adolescent↗
PubMed1983

Visual testing with 'preferential looking' in mentally retarded children.

In 21 out of 26 children and young adults with mental retardation visual acuity could be successfully examined with operant preferential looking. There was a good correlation in this group of patients between ocular abnormalities demonstrated in an ophthalmological examination and the visual acuity results.

Adolescent↗