Biomedical subjects
Publications and source records attributed to G A Rechnitz.
A novel bioselective membrane electrode for L-glutamine has been constructed by coupling living bacteria of the strain Sarcina flava to a potentiometric ammonia gas sensor. Tests in aqueous standards and human serum show that the electrode combines excellent sensitivity and selectivity with rapid response and a useful lifetime of at least 2 weeks.
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A new manual method for the determination of carbon dioxide content in serum and plasma, based on the potentiometric measurement of carbonate, is proposed and evaluated. The new method offers good precision and accuracy in the physiological concentration range as shown by detailed studies on synthetic samples, reference standards, and actual patient samples. Moreover, the method yields results which correlate very well with analyses carried out using various conventional techniques. It is expected that the proposed method could be used for manual determinations of CO2 content, especially where rapid start-up time, minimal sample treatment, and low cost are of importance.
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Direct antibody-hapten binding measurements were carried out with the use of a hapten-selective membrane electrode. Potentiometric titrations of rabbit antibody to the hapten trimethylphenylammonium ion demonstrate the speed and convenience of the new method for the determination of binding equilibria. Average intrinsic binding constants obtained agree well with those measured by equilibrium dialysis techniques.
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A liquid membrane electrode has been developed with Nernstian response and a high selectivity for carbonate ion. The electrode responds rapidly to carbonate in the 10(-2)M to 10(-6)M range with a selectivity for carbonate over chloride, sulfate, and phosphate of at least 10(3) and negligible bicarbonate response. The electrode properties appear to be suited to chemical, biological, chemical, and oceanographic measurements.