Highly stable biosensor using an artificial enzyme.
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Biomedical subjects
Publications and source records attributed to G A Rechnitz.
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The use of intact bacterial cells or tissue slices of plant and animal origin as immobilized biocatalysts has extended the possible range of potentiometric bioselective membrane electrodes beyond that of conventional enzyme electrodes. The use of such materials as biocatalysts offers advantages in situations where isolated enzymes are not available or where multistep reaction paths are required. The resulting bioselective electrodes also offer exceptional ease of preparation, time stability, and low cost.
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Direct antibody-sensing membrane electrodes have been developed by immobilizing ion-carrier immunogen conjugates in a liquid membrane matrix. The resulting potentiometric probes measure specific antibodies with high selectivity over nonspecific antibodies in the physiological pH range. The electrode response is shown to arise from the selective interaction of the antibody with the membrane-bound immunogen.
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