Search PubMed⌕ Search

Biomedical subjects

F Vogel

Publications and source records attributed to F Vogel.

At least 145 records · Page 8Linked to original sources
PubMed1984

Short-leg syndrome.

Short-leg syndrome, or asymmetry of length in the lower extremities, is a common orthopedic problem in children. It is vital to recognize normal bone function, bone growth, and potential growth when evaluating this syndrome. Classification of this problem is either structural or functional. Structural (real) short legs usually have trauma or congenital growth inequality as their etiology. Functional (apparent) short legs usually result from soft tissue contractures or foot function aberrations. The child's age determines the extent of our examination. The lower extremity should be segmented during examination to help determine the location of pathology. Measuring the deformity requires precise scientific procedures. The level of compensation must be determined. Foot, pelvic, and spinal compensations should be evaluated. Scanograms or orthoroentgenograms are useful in diagnosing, quantifying, and prognosing short-leg syndrome. Prediction of the projected discrepancy is accomplished by the Anderson et al. remaining growth charts. Common etiologic considerations include congenital, neuromuscular, infection, trauma-induced, and tumor-caused disorders. Treatment of short-leg syndrome is determined by classification. Structural problems may need heel elevation or a combination of heel elevation and orthotic control on a conservative basis. Functional problems may require neutral position control of the feet with orthotics and correction of soft tissue contractures. Resin foam or orthopedic shoe adjustment may be used in moderate discrepancies. Structural leg inequalities may be corrected by surgical epiphysiodesis. This bone growth retardation procedure is normally performed on the long limb in pediatric patients. Therapy is directed at correcting pelvic obliquity, gait and postural aberration. The end result should be a child with cosmetically acceptable and normal functioning lower extremities.

Child↗
PubMed1983

Fluorescence labelling of NADPH-cytochrome P-450 reductase with the monobromomethyl derivative of syn-9,10-dioxabimane.

The kinetics of thiol-group alkylation in NADPH-cytochrome P-450 reductase during its inactivation by monobromobimane has been studied using the fluorimetric determination of S-bimane-L-cysteine by high-performance liquid chromatography. Loss of activity during the reaction of NADPH-cytochrome P-450 reductase with monobromobimane is caused by the alkylation of one single critical cysteine residue, which can be protected against thiol-specific reagents by NADP(H). The chemical stability of the bimane group allows the digestion of bimane-labelled NADPH-cytochrome P-450 reductase by CNBr. The critical cysteine residue could be located in a CNBr-cleaved peptide purified to homogeneity with Mr 10 500 +/- 1 000 and valine as N-terminus.

Amino Acids↗
PubMed1983

[Microorganisms in home-care inhalation devices].

Contamination of inhalation equipment used at home, cleaning procedures, and the occurrence of potentially pathogenic micro-organisms in mouthwash water was studied in 55 patients. Fungi in the oropharyngeal space were found in 58%, Staphylococcus aureus in 29%, Enterobacteriaceae in 47% and Pseudomonas aeruginosa in 11%. Contamination with potentially pathogenic micro-organisms occurred in 64% of inhalation equipment and inhalation solutions. In a total of 151 samples from the equipment Klebsiella was present in 14%, Enterobacter in 10%, Serratia and Acinetobacter in 11% each, Pseudomonas in 5% and Staphylococcus aureus in 4.5%. In three cases the same serotype of Ps. aeruginosa was present in the oropharyngeal cavity and inhalation equipment. In most cases the cleansing of the inhalation equipment had been inadequate. Only 36% of those questioned cleaned the equipment immediately before use, only 4 of 20 stated that they washed their hands before use. Subjective assessment of the value of inhalation treatment was either "very good" or "good" in 80%.

Aerosols↗
PubMed1983

Is there a general relationship between estimated chromosome distances in interphase and location of genes with related functions?

The problem of a possible clustering of human chromosomes containing genes with related functions was examined in the interphase nucleus of lymphocytes by a statistical comparison of distances between chromosomes containing such functionally related genes with all sets of chromosome distances. The gene locus assignments were taken from a recent review (McKusick 1982); the chromosomal distances were those estimated by Hager et al. (1982) from the frequencies of reunion figures between specific chromosomes as observed in chromosome instability syndromes (Fanconi anemia, Bloom syndrome) and after treatment with Trenimon. Chromosomal distances had been estimated by multidimensional scaling. There was no general tendency for closer location of chromosomes containing genes with related function. A few such chromosomes do show below average distances but this could easily be a chance result.

Cell Nucleus↗
PubMed1983

A linkage study of acrokeratoelastoidosis. Possible mapping to chromosome 2.

As evidenced by a large pedigree with 21 affected members, acrokeratoelastoidosis (AKE) is an autosomal dominant skin disease (10185; McKusick 1978). Linkage with genetic markers already assigned to human chromosomes could help to map the gene for this disease. Therefore 22 markers were investigated in 61 members of the AKE family. Loose linkage is possible between AKE and ACP1, IGKC, and Jk, but the estimated recombination fractions do not reach significant deviations from 0.5. However, since the three marker loci have been previously assigned to chromosome 2, the AKE locus might be assigned tentatively to the same chromosome. Of the provisionally and inconsistently assigned markers, only blood group P is seen to be in linkage with HLA.

Acid Phosphatase↗
PubMed1983

Genetic control of platelet glutaminase: a twin study.

The extent of genetic determination of platelet glutaminase was evaluated by sampling 13 monozygotic and 11 dizygotic adult male twin pairs. Intraclass correlation coefficients of 0.96 for monozygotic and 0.53 for dizygotic twins together with high heritability estimates indicate a strong genetic component.

Blood Platelets↗
PubMed1983

DNA of the Streptomyces phage SH10: binding sites for Escherichia coli RNA polymerase and denaturation map.

Escherichia coli RNA polymerase bound to Streptomyces phage SH10 DNA was visualized by electron microscopy. Six specific binding sites were observed at map units 53, 85, 93, 97, 98, and 99 on the physical map of the 48 kb long genome. Electron microscopy of partially denatured SH10 DNA revealed a characteristic melting pattern of A + T-rich regions around map units 1, 3, 48, 52, and 99. A comparison of the denaturation map with the RNA polymerase binding sites indicates that three binding sites are located in the most A + T-rich regions, two in other early melting regions and one in a segment of higher DNA helix stability.

Bacteriophages↗
PubMed1983

Studies on the SV40-like papovavirus SV40-GBM. I. Genomic analysis by restriction endonucleases and electron microscopy after propagation in CV-1 monkey cells.

Infection of CV-1 monkey cells with SV40-GBM, a papovavirus isolated from a human glioblastoma multiforme, resulted in the appearance of defective viral DNA molecules. In contrast to SV40 wild-type, two main types of variant DNA molecules could be found after three viral passages at multiplicities of infection of about 10. The molecules of one variant DNA (GBM3-L) were about 19% shorter than the GBM3-H DNA molecules and the DNA of the original GBM isolate, as demonstrated by electron microscopy. Restriction enzyme analysis revealed that GBM3-L DNA had lost both the EcoRI and the HpaII cleavage sites which are located in the late viral genome region. Furthermore, SV40 GBM3-L did not possess the two PvuII sites which are located in the late genome region, and a portion of the GBM3-H and GBM3-L DNA molecules had lost the unique KpnI site. Heteroduplex analysis verified that the rearrangements in the GBM3-L DNA are located only in the late region of this DNA. The possible differences between SV40 wild-type and SV40-GBM are discussed on the basis of these results.

Animals↗
PubMed1983

Effect of midazolam on sleep of insomniacs.

We studied in a sleep laboratory the effects of bedtime 15 mg midazolam and 20 mg midazolam on the sleep and morning performance of healthy subjects with polygraphically verified sleep onset insomnia. Six subjects received 15 mg midazolam and six subjects received 20 mg midazolam for 14 consecutive nights which were preceded by a three-night placebo baseline and followed by a three-night placebo period. The medications were administered in a double-blind manner. The results were that both doses increased total sleep time, reduced sleep latency, reduced wake time after sleep onset and reduced the number of awakenings. There was no difference between the doses. Midazolam had its main effect by decreasing wake time in the first third of the night. We found no evidence of tolerance, drug withdrawal rebound insomnia, or drug-induced morning performance decrements. We did find evidence of an anterograde amnesia produced by the drug.

Adult↗
PubMed1983

Evolutionary relationships between papovaviruses and their hosts.

The papovaviridae family consists of two genera, the papillomaviruses (PV) and the polyomaviruses (Py-V). Both genera are distinguished by morphological (larger sizes of the PV) and several biological characteristics. The genomes of either of the two genera share highly conserved DNA regions and a common antigenic determinant, located in their major capsid polypeptides. On the basis of these data an evolutionary relationship among the members of PV and Py-V, respectively, has been suggested. No homology has been found for either DNA- or protein sequences between PV and Py-V and the question of a common ancestor for both viral genera remains open. We have started to characterize the genome of a papilloma producing papovavirus of the Syrian hamster (HaPV). Most of the known biological characteristics of the HaPV suggest it should be classified as a papilloma-like virus. However, the molecular weight of about 3.5 X 10(6) daltons found for the circular duplex DNA lies within the range given for SV 40 and polyoma virus (Py). Analysis of the HaPV genome by cleavage with 21 different restriction endonucleases, location of specific binding sites of phage T 4 gene 32 protein and E. coli RNA polymerase on the viral DNA demonstrated that the HaPV differed distinctly from all other currently known papovaviruses. The HaPV genome was also analyzed by filter hybridization and electron microscopy under conditions of varied stringency for nucleotide sequence homology with the genomes of different papovaviruses of both genera. Whereas no homologous DNA regions could be found between the genomes of HaPV and the human PV types 1 and 4, only under nonstringent conditions (Tm-43 degrees C) stable hybrids were formed between HaPV-, SV 40- and the DNA of a PV isolated from Mastomys natalensis (MnPV). On the other hand extensive homology was detected between the genomes of HaPV and Py even under stringent hybridization conditions (Tm-28 degrees C). The homologous DNA segments mapped on the Py and partially on the SV 40 genome were found to be the most strongly conserved DNA regions among the Py-V genus. These results are discussed with respect to a classification of the HaPV within the papovaviridae family.

Animals↗
PubMed1983

Utmost cytoplasmic location of catalytic center in Na,K-motive ATPase disfavors Mitchell's phosphate-cation symport mechanism of Na/K transport across plasma membrane.

A combined electron microscopic and graded tryptic dissection analysis of the catalytic protein of pure Na,K-motive ATPase reveals that the aspartyl phosphate residue-carrying catalytic center is located at the utmost radius of the hydrophilic cytoplasmic domain. This makes the cation-phosphate symport mechanism of Na/K transport across plasma membrane as proposed by Mitchell topologically unrealistic, and indirectly favors the mechanism of conformational coupling between the catalytic and ionophoric function of the transport system.

Animals↗
PubMed1983

[Antibiotic therapy in severe infections].

The problems of selecting antibiotics for hospital use from the broad variety offered, and the problems of what is often necessarily a non-specific initial therapy, are discussed. A review is given of groups of antibiotics which are important for hospital therapy such as penicillins, aminoglycosides, metronidazole and cephalosporins. Firstly the range of effects of the individual groups of antibiotics is summarized in a simplified form from which the indications are then derived. In discussing the clinical use of antibiotics special importance is attached to initial therapy prior to or in the absence of identification of the pathogens in fatal infectious diseases. From this aspect the therapy for pneumonia, peritonitis, sepsis and urosepsis as well as meningitis is discussed. The small number of indications for the prophylactic use of antibiotics is briefly dealt with. Finally a selection of antibiotics necessary for hospital therapy is presented.

Aminoglycosides↗
PubMed1982

A comparison of the phage T4 gene 32 protein and Escherichia coli RNA polymerase binding sites on hamster papovavirus DNA.

Phage T4 gene 32 protein and Escherichia coli RNA polymerase were bound to hamster papovavirus DNA. The binding regions were identified by electron microscopy employing a protein-free spreading technique. After gene 32 protein treatment four denaturation regions could be mapped, at 0.04-0.12, 0.30-0.36, 0.50-0.60 and 0.75-0.90 DNA map units, respectively, using the unique BamHI cleavage site as zero point. Eight RNA polymerase binding sites can be found which are localized at positions 0.05; 0.11; 0.18; 0.31; 0.57; 0.66; 0.76 and 0.82. A comparison of the RNA polymerase binding sites with the gene 32 protein denaturation pattern reveals a correspondence of six of eight polymerase binding sites with (A+T)-rich regions within the hamster papovavirus genome.

Binding Sites↗
PubMed1982

Position of chromosomes in the human interphase nucleus. An analysis of nonhomologous chromatid translocations in lymphocyte cultures after Trenimon treatment and from patients with Fanconi's anemia and Bloom's syndrome.

The problem of localization of chromosomes in relation to each other in the interphase nucleus of human lymphocytes was investigated by analysis of chromatid and chromosome aberrations observed in lymphocyte cultures of three patients with Fanconi's anemia, one patient with Bloom's syndrome, and in Trenimon-treated (Trenimon, Bayer) normal cells. Distribution of open gaps and breaks is highly correlated with chromosome length and distribution of breaks involved in chromatid translocations in Fanconi's anemia and in Trenimon-treated cells. Both correlations are much lower in Bloom's syndrome. In Fanconi's anemia and in normal cells after Trenimon-treatment, the majority of chromatid translocations are between nonhomologous chromosomes, whereas in Bloom's syndrome mainly homologous chromosomes are involved. Statistical localization of chromosomes in relation to each other in the three-dimensional space by multidimensional scaling gives results consistent with the limited amount of independent evidence.

Anemia, Aplastic↗