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Biomedical subjects

F Uchino

Publications and source records attributed to F Uchino.

At least 73 records · Page 4Linked to original sources
PubMed1984

An ultrastructural and histochemical study of alveolar soft part sarcoma with special reference to the nature of crystals.

We have presented a case of typical alveolar soft part sarcoma probably originating from the soft tissues of the retroperitoneum with metastasis to the brain. An ultrastructural study including histochemical observation of the metastatic lesion was performed to clarify the nature of the crystals characteristic to the tumor cells of this disease. The crystalline structures have acid phosphatase activity at a histological and ultrastructural level. Our results suggested that they might be derived from coalescence of primary lysosomes or by digestive disintegration of some protein.

Acid Phosphatase↗
PubMed1984

Malignant cystosarcoma phyllodes of prostate.

A case of malignant cystosarcoma phyllodes of the prostate is reported in a 45-year-old male. This tumor was composed of benign columnar or squamous cystic folds and sarcomatous stroma including rhabdomyomatous elements. The prostatic origin of the tumor was clearly proved by the unlabeled immunoperoxidase method.

Autoantibodies↗
PubMed1984

Nitrogen-Fixing Bacteria from Warty Lenticellate Bark of a Mangrove Tree, Bruguiera gymnorrhiza (L.) Lamk.

Detached warty lenticellate bark of a mangrove tree species, Bruguiera gymnorrhiza (L.) Lamk. from Iriomote Island, Okinawa, a subtropical region of Japan, showed development of acetylene reduction activity when incubated in a mineral nutrient solution lacking nitrogen under an atmosphere consisting of 5% O(2), 90% N(2), and 5% C(2)H(2). The bacteria responsible for nitrogen fixation were isolated from the bark, and their capacity for acetylene reduction and the incorporation of N(2) into the bacterial cells was confirmed. Four representative strains of the isolates were subjected to taxonomic classification. Two strains were similar to Enterobacter cloacae, and another resembled Enterobacter aerogenes. The characteristics of the fourth strain were similar to those of Klebsiella planticola (Bagley et al., Curr. Microbiol. 6:105-109, 1981). The results of this investigation suggest that the acetylene reduction activity of lenticellate warts of mangrove trunk bark is due to the presence in the warts of nitrogen-fixing bacteria belonging to the family Enterobacteriaceae.

Journal Article↗
PubMed1984

Foamy cells associated with platelet phagocytosis.

In order to gain an insight into the mechanism for the formation of foamy cells (macrophages with foamy cytoplasm) frequently seen in spleens affected by idiopathic thrombocytopenic purpura (ITP), these cells were experimentally induced in mice by subcutaneous injection of platelets with or without accompanied administration of corticosteroid. The light- and electron-microscopic features of experimentally reproduced foamy cells were essentially similar to those seen in the spleens of ITP patients. Corticosteroid had no significant effect on the formation of foamy cells. Most macrophages with foamy cytoplasm contained various amounts of phospholipids, which were derived from platelet membranes. By electron microscopy, myelinlike materials were frequently demonstrated in the cytoplasm of foamy cells. Although lysosomal enzyme activity was revealed in the macrophages that contained morphologically recognizable platelets, there was no demonstrable activity in the cells that contained myelinlike materials. From these results, the following conclusion has been suggested as the mechanism for the formation of foamy cells. Under the state of accelerated phagocytosis of platelets by the macrophages, such as in ITP, the amount of ingested platelet membranes is beyond the capacity of lysosomal digestion. Thus, the incompletely degraded membrane constituents, especially membrane-derived phospholipids, remain in the macrophages, and they are most responsible for the foamy appearance of these macrophages.

Animals↗
PubMed1983

Foamy cells in itp spleens and in granulomas induced by murine platelets, commercialized phospholipids, and erythrocyte membrane. Histological and ultrastructural studies.

A large number of foamy cells were noted in the spleens from fourteen ITP patients, and two patients who received a large amount of platelet rich plasma. Using the unlabelled immunoperoxidase method, these foamy cells were shown to contain platelet antigen. Platelets in varying stages of intracellular digestion, from intact-appearing forms to myelin-like materials, were disclosed in foamy cells. Foamy cells were experimentally induced in granulomas by subcutaneous injection of platelets with or without accompanied administration of steroid, the platelets reacted with anti-murine platelet antibody, commercialized phospholipids (PE, PC, SM, PS, and the mixture of them), and the red blood cell membrane. The foamy cells induced by the subcutaneous injection of platelets are similar to those in the spleens of ITP patients. The lipid in foamy cells is chiefly derived from the membrane phospholipid of injected platelets. Concentric myelin-like materials were also noted in the foamy cells after injection of erythrocyte membrane. The myelin-like materials in these foamy cells are similar to those appearing in macrophages following injection of PC and SM. This suggests that these phospholipids derived from cell membrane are more resistant to intracellular digestion by lysosomal enzymes. We conclude that the foamy appearance of the cordal macrophage in ITP spleens results from incomplete intracellular degradation of platelet membrane.

Adolescent↗
PubMed1982

Amyloid deposits in heart valves.

Localized amyloid deposits were found in 44% of the surgically removed heart valves. They are recognized only in severely destructed valves and the manner of the deposition closely resembles that of aortic intimal amyloid. Although the amyloid fibril protein of the chronically diseased valves is still unknown, it may be neither protein AA nor AL, and another type of amyloid is suggested.

Adolescent↗
PubMed1982

Amyloidosis associated with juvenile rheumatoid arthritis.

Clinical and pathological findings are reported in a Japanese girl who died of secondary amyloidosis associated with juvenile rheumatoid arthritis two years after the onset of symptoms. The patient had intermittent high fever, rheumatoid rash, polyarthralgia, and hepatosplenomegaly. The joints showed the typical histologic changes of juvenile rheumatoid arthritis. Amyloid deposition was found in various tissues; however, remarkable deposition of amyloid was observed in the gastrointestinal tract, especially in the ileum. The amyloid protein in this patient was identified as protein AA using the methods of potassium permanganate treatment and the peroxidase-antiperoxidase unlabeled antibody technique.

Amyloidosis↗
PubMed1979

Erythrophagocytosis by the sinus endothelial cell of the spleen in haemolytic anaemias.

An ultrastructural study of spleens from patients with a heterogeneous group of haemolytic anaemias was undertaken in order to determine whether sinus endothelial cells have erythrophagocytic ability. In most cases, sinus endothelial cells contained erythrocytes and various stages of intracellular degradation of engulfed erythrocytes were noted. Though the frequency of erythrophagocytosis varied from case to case, phagocytosis of erythrocytes by the endothelial cell was more frequent in cases in which cordal macrophages showed active erythrophagocytosis. These results suggest that the sinus endothelial cells have erythropagocytic ability in certain pathological states, especially when the demands for the removal of defective erythrocytes are increased. However, the bulk of erythrophagocytosis is carried out by cordal macrophages, and endothelial phagocytosis has a minor significance in the developemnt of haemolytic anaemia. Two possible processes by which erythrocytes come into contact with sinus endothelial cells are suggested.

Anemia, Hemolytic↗
PubMed1979

Effects of dimethyl sulfoxide and colchicine on the resorption of experimental amyloid.

The induction of amyloid in C3H mice by either casein solution or complete Freund's adjuvant emulsion with Mycobacterium butyricum was confirmed by partial splenectomy. The animals were autopsied after treatment with dimethyl sulfoxide (550 mg/kg, 50 times), colchicine (0.02 mg/kg, 15--37 times), or saline solution as a control. Detailed histological comparisons of biopsy and autopsy spleens provided evidence that dimethyl sulfoxide was significantly effective in the resorption of amyloid, while in the animals treated with colchicine amyloid deposition was increased. The effect of dimethyl sulfoxide was discussed with reference to the modification of amyloid fibrils.

Amyloid↗
PubMed1978

Pathological study on amyloidosis--relationship of amyloid deposits in the aorta to aging.

Senile aortic amyloidosis in 224 autopsy cases over 40 years was investigated comparing cardiac and pancreatic amyloidosis in them. A total of 176 cases of aortic amyloidosis was found for an average incidence of 79%. Under the 5th decade the incidence was 51% and it rose sharply with age and reached 95% in over the 8th decade. The incidence of cardiac amyloidosis also increased with age, but it was always higher in the aorta. Aortic and cardiac amyloid were both positive in the DMAB method for tryptophan. The major part exposed to amyloidosis in the aorta was the media. The medial amyloid consisted of numerous minute deposits and had no relation to atherosclerosis. Some comments about the pathogenesis of senile amyloidosis were also mentioned.

Adult↗
PubMed1978

Subacute sclerosing panencephalitis with special reference to the ultrastructure of inclusions in the brain and lung.

A 7-year-old boy, who was diagnosed as typical SSPE by clinical data and laboratory findings, was autopsied and observed by immunofluorescent techniques, light and electron microscope. The morphological characteristics in the brain were perivascular cuffings with plasma cells, lymphocytes and mononuclear cells, gliosis and a large number of intranuclear and intracytoplasmic inclusions in the neuroglias and nerve cells. Various kinds of intranuclear inclusions were elucidated by electron microscopy and the fin structures of these inclusions were described in detail. At least five types of intranuclear inclusions were regarded as specific in SSPE. The presence of intranuclear inclusions of mononuclear cells in the lungs resembling the inclusions in the neuroglias suggested that the disease was not localized in the brain but could be disseminated throughout the body.

Brain↗
PubMed1978

Fine structure of the spleen in autoimmune hemolytic anemia associated with systemic lupus erythematosus.

The fine structures of the red pulp of the spleen and the liver of a patient with autoimmune hemolytic anemia, neutropenia and thrombocytopenia associated with systemic lupus erythematosus are described. The red blood cells were phagocytized in toto by the splenic macrophages. These also contained neutrophils and platelets in various stages of degradation. Sinus endothelial cells revealed occasional erythrophagocytosis. The Kupffer cells in the liver occasionally contained red cells and platelets. These morphological findings and marked improvement of hematological abnormalities following splenectomy suggested that the spleen was the major site of destruction of blood cells. Undulating tubules associated with the endoplasmic reticulum were present in the sinus endothelial cells of the spleen.

Anemia, Hemolytic, Autoimmune↗