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Biomedical subjects

F Suzuki

Publications and source records attributed to F Suzuki.

At least 505 records · Page 28Linked to original sources
PubMed1984

Regulation of nervous system-specific S-100 protein and enolase levels in adipose tissue by catecholamines.

The effect of catecholamines on the levels of S-100 protein and nervous system-specific enolase (NSE) in epididymal adipose tissue of Wistar rats in vivo was examined by sensitive enzyme immunoassay methods. Soluble S-100 protein levels in the adipose tissue of 9-12-week-old rats (1.46 +/- 0.19 microgram/mg protein) were decreased to less than 50% of those of controls by serial injection (for 4-7 days) of epinephrine (0.1 mg/day) or norepinephrine (0.15 mg) with, however, little effect on the levels of membrane-bound (pentanol-extractable) S-100 protein. A significant decrease in the soluble S-100 protein levels was observed at 2 h after a single injection of epinephrine (1.04 +/- 0.13 microgram/mg protein). On the other hand, levels of NSE subunit (gamma subunit or 14-3-2 protein) in adipose tissue (0.51 +/- 0.03 gamma gamma-equivalent pmol/mg protein) were increased to 170% of control by serial injection (for 7 days) of epinephrine or norepinephrine with little change of the level of enolase alpha subunit on a mg protein basis. Isoproterenol had no apparent effect on the levels of soluble S-100 protein and NSE subunit. These results suggest that the levels of S-100 protein and NSE in adipose tissue are regulated by catecholamines.

Adipose Tissue↗
PubMed1984

Studies on chondrocytes from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis in culture. I. Morphology, growth, glycosaminoglycan synthesis, and responsiveness to bovine parathyroid hormone (1-34).

Methods for isolating chondrocytes from the craniofacial complex and culturing them in vitro were established. Chondrocytes which were isolated by collagenase digestion from mandibular condylar cartilage, nasal septal cartilage, and spheno-occipital synchondrosis grew well in vitro. All three types of chondrocytes actively synthesized glycosaminoglycans, a differentiated phenotype of chondrocytes, and responded well to parathyroid hormone. However, some different characteristics were noted among the three types of chondrocytes in culture.

Animals↗
PubMed1984

Light and electron microscopical observations on the male excurrent duct system of the common shrew (Sorex araneus).

In sexually active common shrews (Sorex araneus) the rete testis is a simple sac-like structure situated superficially between the equator and the anterior pole of the testis, and its epithelium glycogen-rich cells. In the ductuli efferentes, the tight junctions of the epithelial cells are poorly developed. The epididymis can be divided cytologically into initial, middle and terminal segments, and some variations are apparent in the fine structure of the principal cells throughout the duct. In the initial segment and in the most proximal part of the middle segment, mitochondrion-rich cells occur sporadically in the epithelium. The ductus deferens is divided into three anatomical regions, a tortuous pars epididymica, a straight middle portion, and an ampullary region. The distal half of the straight portion is distended, and appears to function, together with the terminal segment of the epididymis, as a sperm reservoir.

Animals↗
PubMed1984

Cultured growth cartilage cells.

Growth cartilage (GC) and resting cartilage (RC) cells from the ribs of young rats were separated and cultured. The cultured GC cells showed remarkable osteogenic potential only with the participation of certain host cells even after cultivation, while RC cells showed no osteogenic activity when transplanted as isografts loaded into Millipore chambers. The GC cells showed marked differences in glycosaminoglycan (GAG) synthesis and alkaline phosphatase activity as compared with RC cells in terms of the effects of various hormones, vitamins, and other agents. A linkage of parathyroid hormone (PTH) and polyamine metabolism was found, and it was suggested that PTH induces successive increase of ornithine decarboxylase activity, polyamine levels, and GAG synthesis in cultured chondrocytes obtained from growing rabbit costochondral junctions. A factor in a family of somatomedins was isolated from the cartilage of fetal calves and called "cartilage-derived factor" (CDF). CDF markedly increased GAG, protein, RNA, and DNA synthesis and cell division of the cultured cartilage cells. The GC cells formed matrix vesicles abundantly in vitro without mineral deposition. Co-culture of GC cells with bone marrow cells resulted in degradation of GAG and then formation of hydroxyapatite crystals in the extracellular matrix. Antirat GC mouse IgG was prepared to label and sort the osteogenic cells in bone marrow by fluorescence-activated cell-sorter II (FACS II). GC antigen-positive and -negative cells after FACS sorting were cultured and examined in terms of proteoglycan synthesis, alkaline phosphatase activity, and matrix vesicle production. The former cell group was found to be very similar to the cultured GC cells.

Animals↗
PubMed1984

[Trigeminal sensory evoked potential in retrogasserian glycerol injection for trigeminal neuralgia].

Since Dr. Håkanson reported retrogasserian glycerol injection method for treatment of idiopathic trigeminal neuralgia, its effectiveness for pain relief has been documented in a few reports. However, effects of glycerol in nervous tissue and mechanism of pain relief of the trigeminal neuralgia have not been elucidated yet. So we attempted to make clear what changes happen in trigeminal sensory evoked potential (TEP) of the patients with trigeminal neuralgia after retrogasserian glycerol injection. For this purpose we had to obtain normal TEP from 12 normal subjects in the beginning. The TEP by upper lip stimulation and recorded at contralateral C5 or C6 (TEP.V2) was consisted of 4 positive and 2 negative potentials in the analysis time of 40 msec which were named P3, N10, P14, P20, N30 and P40. The TEP.V3 that was obtained by lower lip stimulation was consisted of 3 positive and 3 negative potentials that were named N3, P10, N14, P20, N30 and P40. Most remarkable change of the TEP.V2 after glycerol injection in the pain relief group of patients with trigeminal neuralgia involving second division was amplitude attenuation of N10. There was no significant prolongation of peak latencies of P3, N10 and P20. It was remarkable that this amplitude decrease (about 38% of the pre-injection value) was recognized only in the pain relief group. On the contrary the mean amplitude of no relief group decreased only to 86% of the pre-injection value.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1984

[Surgical treatment of atlanto-axial dislocation. An introduction of two new methods].

In the past fourty-two months we have operatively treated twenty cases of several types of atlanto-axial dislocation (AAD) including an atlanto-axial deformity. A transoral retropharyngeal approach to the anterior arch of the atlas and the body of the axis was performed in two cases of AAD with dens fracture, 2 traumatic pure anterior AAD with relatively narrow spinal canal and 1 os odontoideum. The operative method we have devised is a modification of the transoral micro-surgical odontoid resection. In hyperextended position the fractured dens or os odontoideum comes to the position nearest to the anterior arch of the atlas and a distance between the atlas and the axis almost disappears. In this position a Halo device is installed and the anterior arch of the atlas, the dens and the body of the axis are fixed by an autograft all together. The articular joints between the atlas and the axis are also fixed by small grafts. The "three points fixation" of the atlanto-axial vertebrae may be a better and safer operative technique for selected AADs. Another method for AAD is a posterior atlanto-axial fixation using a metal plate (Urlich Medical Instruments Co., West Germany). This technique is a modification of fixation with wire and methyl methacrylate. A strong point of this new method is that the laminae of the atlas, axis and C3 vertebra in some cases, are fixed not with wire but a plate. The wire is used only to fix the plate on the laminae, so that the wire is not snapped on elastic fatigue.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1983

Characterization of alpha alpha, beta beta, gamma gamma and alpha gamma human enolase isozymes, and preparation of hybrid enolases (alpha gamma, beta gamma and alpha beta) from homodimeric forms.

Four forms (alpha alpha, beta beta, gamma gamma and alpha gamma) of enolase (2-phospho-D-glycerate hydrolyase, EC 4.2.1.11) were purified from human brain and skeletal muscle. Each purified preparation showed a single band on SDS-polyacrylamide gel electrophoresis, with a relative mobility corresponding to a molecular weight of about 46 000 for alpha and gamma subunits, or 44 000 for the beta subunit. The four enzymes showed similar values of specific activity (about 80 units/mg), optimal pH (pH 6.8-6.9), and Km for 2-phosphoglycerate (about 3 X 10(-5) M). Amino acid analysis, peptide mapping analysis with a limited proteolysis, and immunochemical studies of the purified enzymes revealed that the three subunits, alpha, beta and gamma, are distinct proteins. Three hybrid enolases (alpha gamma, beta gamma and alpha beta) could be prepared from a mixture of two homodimeric enolases by dissociation and reassociation procedures, followed by isolation with ion-exchange column chromatography.

Amino Acids↗
PubMed1983

Immunoassay of human muscle enolase subunit in serum: a novel marker antigen for muscle diseases.

A sandwich enzyme immunoassay method for measurement of beta subunit of muscle enolase in human serum was developed by use of purified antibodies to enolase beta subunit and beta-D-galactosidase from Escherichia coli as label. The assay was specific to the beta subunit with no cross-reaction with the alpha and gamma subunits of human enolase. The measurable range was from 10 pg to 10 ng per assay tube or 1 to 1000 ng/ml serum. Coefficients of variation within-run and between-run for the assay of serum beta subunit were less than 14%. Normal adult sera contained about 6 ng/ml of the beta subunit, and the levels were significantly elevated in sera of patients with muscular dystrophy and those with myocardial infarction. Serum levels of the beta subunit correlated well with those of creatine phosphokinase, but poorly with those of myoglobin in the same samples. The specific distribution of beta subunit in skeletal muscle and heart was confirmed by measuring the levels in various tissue extracts.

Creatine Kinase↗
PubMed1983

Prostaglandin stimulation of adenosine 3',5'-monophosphate accumulation in cultured chondrocytes in the presence or absence of parathyroid hormone.

The effect of prostaglandin analogues on the cyclic AMP level in cultured chondrocytes were examined. Prostaglandin E1 at 0.4 to 30 microM, increased the intracellular concentration of cyclic AMP in chondrocytes. Its effect was rapid, being evident within 1 min and reaching a maximum in 10 to 20 min. The maximum level was sustained until 30 min after its addition and then decreased gradually. Prostaglandin D2 and E2 also increased the cyclic AMP level in chondrocytes, but they had less effect than prostaglandin E1. Prostaglandin A1 had no effect on the nucleotide level in chondrocytes, although they markedly increased the level in fibroblasts. The time course of stimulation of cyclic AMP accumulation in chondrocytes by prostaglandin E1, D2 or E2 was quite different from that by parathyroid hormone (PTH): the effect of prostaglandin was slower and more sustained than that of PTH. PTH potentiated the effect of prostaglandin E1, E2, or D2 on the cyclic AMP level in chondrocytes and that the combined effects of prostaglandin and PTH were more than additive. Addition of an inhibitor of cyclic nucleotide phosphodiesterase with prostaglandin, PTH or both produced a synergistic effect on the accumulation of cyclic AMP in the chondrocytes. These findings suggest that prostaglandin E1, E2, and D2 increase the synthesis of cyclic AMP and that the combined effect of the prostaglandins and PTH on the cyclic AMP level in chondrocytes is partly attributed to the synergistic synthesis of cyclic AMP in the cells.

1-Methyl-3-isobutylxanthine↗
PubMed1983

Immunoassay of three enolase isozymes in human serum and in blood cells.

Sandwich enzyme immunoassay procedures for measurement of three human enolase isozymes (alpha alpha, alpha gamma and gamma gamma forms) were developed by use of purified antibodies specific to the alpha or the gamma subunit of enolase. The assay systems consisted of polystyrene balls with immobilized antibody F(ab')2 fragments and antibody Fab' fragments labeled with beta-D-galactosidase from E. coli. The measurable range was from 3 pg to 10 ng of each enolase isozyme per assay tube, and the levels of the three enolases in human sera and isolated blood cells were determined. Serum samples from healthy adults contained about 60, 8, and 2 ng/ml of alpha alpha, alpha gamma, and gamma gamma enolases, respectively. Red blood cells and platelets had about 4, 1, and 0.1 ng/10(6) cells of alpha alpha, alpha gamma, and gamma gamma enolases, respectively. Lymphocytes (mononuclear cells) contained larger amounts of all three enolases (alpha alpha, 370 ng/10(6) cells; alpha gamma, 30 ng/10(6) cells; gamma gamma, 2.7 ng/10(6) cells). The levels of three enolase isozymes in sera of patients with small-cell cancer of the lung were also determined.

Blood Platelets↗
PubMed1983

Differential and synergistic actions of somatomedin-like growth factors, fibroblast growth factor and epidermal growth factor in rabbit costal chondrocytes.

Epidermal growth factor (EGF) stimulated DNA synthesis, RNA synthesis and cell division of rabbit costal chondrocytes in culture, but did not stimulate nor inhibit proteoglycan synthesis. Multiplication-stimulating activity (MSA) and cartilage-derived factor (CDF) with somatomedin-like activity had relatively small effects on DNA synthesis and cell division of chondrocytes, but markedly enhanced the synthesis of sulfated proteoglycans. MSA and EGF had additive or synergistic effects on the growth of rabbit chondrocytes. MSA and fibroblast growth factor (FGF) also had additive or synergistic effects on the growth of rabbit chondrocytes. CDF mimicked these effects of MSA; CDF and MSA had similar stimulatory effects on DNA synthesis in rabbit chondrocytes even in the presence of EGF, FGF or both. MSA and CDF did not reduce the concentrations of EGF and FGF required for maximum stimulation of DNA synthesis. In addition, EGF and FGF had additive effects on DNA synthesis and cell division of rabbit costal chondrocytes in culture. These findings suggest that the somatomedin-like factors, EGF and FGF have complementary effects in chondrocytes and provide further evidence that CDF produced by chondrocytes resembles somatomedin in its biological action in chondrocytes.

Animals↗
PubMed1983

Freeze-fracture observations of ovulated hamster oocytes with their cumulus cells.

Mature unfertilized oocytes in the cumulus oophorus were examined using the freeze-fracture technique. The P face of the oocyte plasma membrane (oolemma) has randomly distributed intramembranous particles (IMPs) with an average diameter of about 10 nm. No marked difference is found between the intermicrovillous and microvillous regions of the oocyte with respect to the size and distribution of IMPs. The E face of the oolemma has fewer IMPs than the P face. The intermicrovillous region is slightly elevated in some places because of close contact with underlying cortical granules. The oolemma of this elevated region and the membrane of the cortical granule have IMPs which are considerably smaller than those in the remaining areas of the oolemma. The IMP density of the P face of the cortical granule membrane is much lower than that of the E face, possibly indicating a membrane specialization required for fusion of the cortical granule membrane with the overlying oolemma at the time of exocytosis. Cytoplasmic lamellae appear as parallel bands of particles in freeze-fractured specimens. No gap junctions are found between the oocyte and cumulus cells, but residual gap junctions are seen among clustered cumulus cells.

Animals↗
PubMed1983

Simultaneous radical surgical treatment for membranous obstruction of the inferior vena cava and the coincident hepatocellular carcinoma--the first successful case.

To our knowledge, this is the first report of a patient for whom a successful radical treatment for both membranous obstruction of the inferior vena cava (MOVC) and coincident hepatocellular carcinoma (HCC) were simultaneously carried out. A 36 year old Japanese man with Budd-Chiari syndrome due to MOVC was found to have HCC in the right hepatic lobe during a shunt operation between the inferior vena cava (IVC) and the right atrium (RA), using a vascular prosthetic graft. He was referred to us from another institute for further operative procedures. Both the mediastinum and the abdomen were entered through a long midline incision with longitudinal splitting of the sternum. The graft that had been occluded by thrombus was removed. Transcardiac membranotomy was achieved by manual manipulation and then by Tubb's dilator. The hepatic tumor with an abundant surgical margin of the liver was resected. The tumor of 2.5 X 2.5 X 2.0 cm was a well differentiated clear cell type HCC associated with congestive liver cirrhosis. Postoperative contrast and pressure studies through IVC showed satisfactory results. Serum alfa-fetoprotein levels decreased from 503 to 12 ng/ml. The patient is well at least for 44 months after the surgery without any recurrence at the time of completion of this writing.

Adult↗
PubMed1983

S-100 protein in adipose tissue.

1. The nervous system-specific S-100 protein is present at high levels in adipose tissues of various animal species. 2. The adipose S-100 protein levels in beef and rat were 1.13 and 1.59 micrograms/mg protein, respectively, which were comparable to the brain levels (beef, 4.85 micrograms/mg; rat, 1.98 micrograms/mg). 3. Adipose tissues of rabbit, pig and human contained 0.222, 0.149 and 0.083 micrograms S-100 protein/mg, respectively. 4. The S-100 protein level in rat adipose tissue showed a developmental profile similar to that in the brain.

Adipose Tissue↗
PubMed1983

Restoration by parathyroid hormone and dibutyryl cyclic AMP of expression of the differentiated phenotype of chondrocytes inhibited by a tumor promoter, 12-0-tetradecanoylphorbol-13-acetate.

12-0-Tetradecanoylphorbol-13-acetate (TPA) inhibited expression of the differentiated phenotype of chondrocytes in rabbit costal chondrocytes in culture. TPA transformed typical polygonal chondrocytes into multilayered, fibroblastic cells and also inhibited the rate of [35S]sulfate incorporation into glycosaminoglycan (GAG), a differentiated phenotype of chondrocytes. These changes were apparent within 24 h and reached a plateau at 48 h after the addition of TPA. Phorbol didecanoate and phorbol dibenzoate also inhibited sulfation of GAG, even though the effect was weaker than that of TPA. Phorbol diacetate and 4-0-methyl TPA did not inhibit sulfation of GAG. Addition of parathyroid hormone (PTH) or dibutyryl cyclic AMP simultaneously with TPA overcame the inhibition caused by TPA. PTH and dibutyryl cyclic AMP also reversed the inhibition and stimulated expression of the differentiated phenotype of chondrocytes even in de-differentiated cells which had been pretreated for 3 days with TPA. These findings suggest that cyclic AMP plays an important role in the restoration of the differentiated phenotype of chondrocytes in TPA-treated chondrocytes, and that the TPA-treated cells retain some of the differentiated phenotype of the original cells, such as responsiveness to PTH.

Animals↗
PubMed1983

Suppressive action of near-ultraviolet light on ouabain resistance induced by far-ultraviolet light in Chinese hamster cells.

The interaction between ultraviolet light (UV-C) from germicidal lamps (254 nm) and near-ultraviolet light (UV-B) from Westinghouse Sun Lamps (290-345 nm) was studied in Chinese hamster V79 cells by measuring the effectiveness of combined exposures to induce the resistance to 6-thioguanine or to ouabain. Exposure of cells to a conditioning dose of UV-B (approximately 70% survival) results in significant inhibition of the induction by UV-C of cells resistant to ouabain. The inhibition is lost, however, if cells are incubated for 12 h at 37 degrees C between exposures. Inhibition is also observed when cells are preirradiated with a dose of UV-B filtered with polystyrene (300-345 nm) which, in itself, has no effect on cell killing. Conditioning exposures of unfiltered or filtered UV-B light do not inhibit the induction of 6-thioguanine-resistant cells by UV-C light, and the effects of UV-B and UV-C light are largely independent.

Animals↗