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Biomedical subjects

F Sorice

Publications and source records attributed to F Sorice.

At least 73 records · Page 4Linked to original sources
PubMed1978

[Determination of antibodies against influenza viruses, using the ELISA method].

A microplate method of enzyme-linked immunosorbent assay (ELISA) was adapted for the demonstration of antibodies to influenza A and B in 25 paired acute and convalescent sera taken from patients with influenza A infection, in 11 single convalescent sera collected from patients with acute respiratory infection and serological evidence of influenza B infection, in 14 paired sera collected from volunteers who were vaccinated with an aqueous inactivated influenza A and B vaccine, and in 15 serum samples from normal subjects. Comparison showed that ELISA was more sensitive than the hemagglutination-inhibition test. The enzyme-immunoassay gave equally reliable and reproducible results and requires very small amounts of antigen and sera. This simple test may be suitable for use in large seroepidemiological surveys.

Antibodies, Viral↗
PubMed1977

[The ELISA test in immunodiagnosis of human hydatidosis. Preliminary observations].

An evaluation of the E.L.I.S.A. method, compared to the indirect hemagglutination (I.H.) and to the counterimmunoelectrophoresis (CIEP) tests for the biological diagnosis of hydatidosis, was performed on 31 sera of patients, carrier of hydatid cysts, on 17 sera of patients with other infectious and parasitic diseases, and on 7 sera of blood donors. The immunoenzymatic test turned out positive in 30 out of the 31 sera of the patients with active hydatidosis (96.7%), while IH and CIEP turned out positive in 29 cases (93.5%). The control sera from patients with other diseases proved to be all negative, except one from a patient with active schistosomiasis. E.L.I.S.A. test was constantly negative with the sera of normal subjects. The validity and usefulness of E.L.I.S.A test for the diagnosis of the hydatid disease is discussed.

Counterimmunoelectrophoresis↗
PubMed1975

[Behavior of serum IGE in human hydatidosis].

The quantitative assay of serum IgE was performed in 34 patients with active hepatic and extrahepatic hydatidosis, in 10 subjects with previous hydatidosis, who underwent surgery during the preceding 8 years, without clinical signs of relapse, and in 15 normal bloods donors. To this purpose a new type of plate from the market was employed, which, adopting radial immunodiffusion, allows determining quantitatively the IgE serum values at the upper normal limits (800 I.U./ml) besides higher levels. IgE serum concentrations, higher than 800 I.U./ml, were detected in 33 (97.06%) out of the 34 patients with active hydatidosis, in 5 (50%) out of the 10 subjects with previous hydatidosis, in 4 (26.67%) out of the 15 normal subjects. The statistical analysis of the results showed the significance of the comparison between the IgE values observed among the group of the patients with active hydatidosis and those, respectively, of the subjects with previous hydatidosis (p less than 0.05) and of the normal subjects (p less than 0.001). The results obtained are discussed in the light of the most recent knowledge on the role played by IgE in the complex immune mechanism of parasitic diseases.

Echinococcosis↗
PubMed1975

[Further aspects of combination antibiotic therapy. Critical review and personal case studies].

A brief account of the aims sought by multiple antibiotic management is followed by an assessment of the antagonism and synergism displayed by associations of two bactericidal antibiotics, two bacteriostatic antibiotics, and one bactericidal and one bacteriostatic antibiotic. Instances of synergism between bactericides (particularly penicillins and aminosides) are mentioned. Stress is laid on recent studies on the mechanism of action of antibacterial drugs showing unmistakeable synergism between trimethoprim and sulphamethoxazol and between chloramphenicol and tetracycline. The antagonism between bactericides and bacteriostatics noted by Jawetz et Al. has not been confirmed clinically in a number of reported series. The main indications for combined antiobiotic therapy are reviewed: endocarditis, purulent meningitis, staphylococcia, brucellosis, salmonellosis, shigellosis, other Gram-negative infections and fever in the course of blood diseases. References is made to personal experience in the management of 35 cases of bacterial endocarditis, 15 cases of purulent meningitis and various forms of serious Gram-negative infection. Leaving aside exceptional cases, the clinical effects of antibiotic associations are uncertain and influenced by too many variables. The technique is still of importance, however, despite the introduction of many new antibiotics. It must not be thought of as a handy method for indiscriminate use, however; its indications (which are summarised) are quite clear.

Adult↗
PubMedDate not supplied

Cerebrospinal fluid antiganglioside antibodies in patients with AIDS.

In this study the presence of brain antiganglioside antibodies in the cerebrospinal fluid (CSF) of patients with HIV infection was analysed. CSF samples were collected from 45 patients with AIDS and from 45 anti-HIV negative subjects, 15 of whom presented aseptic meningitis. Nineteen AIDS patients had clinically well-documented encephalopathy. Thirteen of these patients had white matter lesions shown by magnetic resonance imaging (MRI). Both IgG and IgM antiganglioside antibodies were detected by immunostaining on thin layer chromatography plates in three CSF samples from AIDS patients with progressive encephalopathy with signs of a diffuse demyelination, as revealed by MRI. Two of these CSF samples reacted specifically with GM3, GM1 and GD1a and one with GD1a. In none of the HIV infected patients without demyelinating encephalopathy, but with opportunistic infections or cerebral lymphoma, nor in the anti-HIV negative control subjects were antiganglioside antibodies detected. No association with JCV DNA, CMV DNA, EBV DNA, detected by nested PCR, nor HIV antigen p24 was found. These findings show the presence of brain antiganglioside antibodies in the CSF of AIDS patients for the first time. However, the findings do not suggest relating the presence of these antibodies to HIV encephalopathy or particular viral agents, but indicate that the antibodies are detectable in subjects with progressive encephalopathy with a diffuse demyelination.

Acquired Immunodeficiency Syndrome↗
PubMedDate not supplied

Human eosinophil heterogeneity.

The aim of this review is to examine some of the most important findings pointing toward human eosinophil heterogeneity. Special importance is given to cell density, surface receptors, metabolism and response to biological products or synthetic compounds.

Eosinophils↗
PubMedDate not supplied

Immunological and viral markers of HIV infection and retinal microangiopathy.

The relationship between retinal microangiopathy and some features of human immunodeficiency virus (HIV) infection such as HIV antigenemia, antibodies to the viral proteins, T lymphocyte subsets, were studied in 71 patients with acquired immunodeficiency syndrome (AIDS). The absence of antibodies to the HIV p24 protein was significantly related to retinal microangiopathy (p = 0.0051) and more closely to retinal cotton-wool spots (p = 0.0007); the combination of positive antigenemia with the absence of antibodies to p24, which is typical of the later phases of HIV infection, was found in a larger percentage of patients with cotton-wool spots (p = 0.0013) than in subjects with every sign of microangiopathy (p = 0.0546). T-helper (CD4+) cells count below 200 cells/mm3 was also detected in a higher percentage of patients with HIV-related retinal microangiopathy (p = 0.009). These findings suggest that retinal microangiopathy and especially retinal cotton-wool spots are related to the progression of immunodeficiency.

AIDS-Related Opportunistic Infections↗
PubMedDate not supplied

[The enzyme-linked immunosorbent assay (ELISA) in the diagnosis of amoebiasis (author's transl)].

An enzyme-linked immunosorbent assay (ELISA) technique as an aid to the detection of antibodies to Entamoeba histolytica has been evaluated in four groups of 39 individuals. Results were positive in 15 patients with amoebic dysentery harboring trophozoites of Entamoeba histolytica, in their stools; negative in 6 patients with enteric symptoms whose faeces contained neither trophozoites nor cysts of Entamoeba histolytica, in 8 patients with other parasitic diseases in whom clinical examination was not indicative of infection with Entamoeba histolytica, and in 10 sera from blood-donors. The ELISA has been shown to be a sensitive method of detecting antibodies to entamoeba histolytica.

Amebiasis↗
PubMedDate not supplied

Spectrum of HIV infection and AIDS in a cohort of Italian hemophiliacs.

A group of 173 subjects affected by congenital clotting factor deficiencies was evaluated with regard to the impact of HIV infection. On the whole, 78 patients (45%) were found to be HIV Ab-positive. As of March, 1988, of the seropositive patients, 63 (80.8%) had an asymptomatic HIV infection (Group II/CDC), three (3.8%) had a persistent generalized lymphadenopathy (Group III/CDC). The 12 (15.4%) remaining patients could be classified in Group IV of the CDC classification due to their symptoms and signs; in particular, 10 came under surveillance case definition for AIDS. Assay for the HIV antigen was positive in 14 (17.9%) seropositive hemophiliacs. With regard to the immunological features, our data clearly show that a sharp decline in the number of CD4+ cells was associated with symptomatic forms of the disease. An evaluation of the time elapsed from seroconversion to the appearance of the symptomatic clinical condition showed an average incubation of 37 months.

Acquired Immunodeficiency Syndrome↗