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Biomedical subjects

F Schunter

Publications and source records attributed to F Schunter.

48 records · Page 3Linked to original sources
PubMed1977

Detection and quantitation of human Ia-type antigens with iodinated protein A and specific purification of antibodies against Ia-type alloantigens.

Antibodies directed against specific human Ia-type antigens can easily be detected and quantitated by an improved radioimmunoassay using iodinated protein A bound to a specific antibody-Ia-antigen complex on the surface of freshly drawn peripheral human leukocytes, cultured human cell lines, or lymphoid cells fixed with glutardialdehyde or formaldehyde. The same principle can also be used for the detection of Ia alloantigens on human lymphocytes when testing them with specific antisera known to contain antibodies against transplantation antigens. These anti-Ia-alloantigen antibodies had been purified by a two-step procedure involving ion-exchange chromatography on DEAE-cellulose at pH 6.3 and the specific absorption on formaldehyde-fixed Ia-alloantigen-carrying homozygous cell lines, followed by elution of these antibodies with isotonic citrate buffer at pH 3.0. In this way an about 90-fold purification could be achieved. After such a purification the highly enriched antibody fraction still reacted selectively with one specificity of the Ia antigen system.

Cell Line↗
PubMed1977

Map order of the linkage group GLO-Bf-HLA-A-PGM3 on human chromosome 6.

Linkage analysis of 52 families with 181 children confirms close linkage for GLO-Bf, GLO-HLA-A, and Bf-HLA-A and proves linkage between HLA-A and PGM3. Linkage for Bf-PGM3 and GLO-PGM3, respectively, seems to be likely; close linkage can be excluded. From the relative map distances, but above all from the segregation of defined linkage phases in crossover families, we can hypothesize that the sequence order of the loci should be GLO-Bf-HLA-A-PGM3.

Chromosome Mapping↗
PubMed1977

Apparent lack of gene dosage effect in the PLT assay.

In this paper the results of primed lymphocyte typing (PLT) tests are reported which show the response to HLA-D-specific stimulator and to unrelated cells. The aim was to find out the sensitivity of the PLT method for evaluating not only qualitative HLA-D-region differences but also gene dosage effects. In addition, the primed cells were restimulated with cells from HLA-D-homozygous individuals mutually negative with the specific stimulator in the usual 5-day primary mixed lymphocyte culture incubation. No gene dosage effect in PLT experiments could be observed.

Epitopes↗
PubMed1975

[Histocompatibility-antigens in hay fever (author's transl)].

HL-A antigens have been determined in 110 not related Caucasian patients from southwest Germany suffering from hay fever and in 353 blood donors from the same area. Gene-frequencies and haplotype-frequencies of these 2 groups were compared, deviations within various specific allergy groups were investigated taking into consideration associated diseases. Patients suffering from hay fever showed a significant increase in frequency of W 19 and a significant decrease in HL-A8. Increase of W 19 was most evident in persons who became sick before the 16th year of age and in those who showed asthmatic symptoms in addition to those of hay fever. HL-A8 is significantly decreased only in persons who became sick after the 16th year of age. Found differences in haplotype-frequencies need further confirmation in larger studies. A relationship between changes of HL-A frequencies in hay fever due to special pollen allergies could not be determined.

Adolescent↗