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Biomedical subjects

F Schunter

Publications and source records attributed to F Schunter.

At least 37 records · Page 2Linked to original sources
PubMed1987

HIV-specific antibody among voluntary blood donors in Lower Saxony (FRG).

Anti-HIV test results of the Red Cross Blood Transfusion Service of Lower Saxony from 1 June 1985 to 31 July 1986 inclusive were analysed retrospectively. Nine out of 70,936 donors who had not donated blood before 1 June 1985 (first-time donors) and 9 out of 261,231 donors who had donated blood before this date (repeating donors) were found anti-HIV confirmed positive at the time of the first blood donation during the study period. The prevalence of HIV antibody in first-time donors was significantly higher than in repeating donors (p less than 0.01). It was concluded that some members of risk groups used blood donation to obtain an anti-HIV test result. One out of 30,300 blood donations was confirmed anti-HIV positive. The results of this study justify the transfusion of blood donations that are reactive only in the initial ELISA test.

Antibodies, Viral↗
PubMed1985

Linkage between the loci for mitochondrial malic enzyme (ME2) and coagulation factor XIIIA subunit (F13A).

The locus for the mitochondrial malic enzyme (ME2) seems to be closely linked to the gene coding for the A subunit of coagulation factor XIII (F13A). From 18 informative families with 54 children a maximum lod score of 4.33 was obtained at a recombination fraction of 0.10. Since the assignment of F13A to the short arm of chromosome 6 was recently confirmed, the locus for ME2 can be added to this linkage group. The map order seems to be: GLO1 - HLA - F13A - ME2.

Adult↗
PubMed1985

DNA polymorphism of the major histocompatibility class I genes and their association with serologically defined haplotypes.

DNA of unrelated persons as well as members of families that were totally or partially homozygous or completely heterozygous on the loci of the major histocompatibility class I genes has been isolated from peripheral blood lymphocytes and blot hybridized with the class I pseudogene pHLA 12.4 probe. The autoradiographic DNA patterns were discussed and compared with well-defined serological features. Positive associations with serologically typed alleles had been demonstrated for HLA-A1,11; -A2; -A3; -B7; -B14; -B35; -Bw41; and -Cw5.

Alleles↗
PubMed1985

Characterization of a monoclonal anti-Bw4 antibody (Tü109): evidence for similar epitopes on the Bw4 and Bw6 antigens.

The production and serologic, as well as immunochemical properties of a cytotoxic murine IgG monoclonal antibody (Tü109) that precipitates HLA-class I molecules, are described. In the microcytotoxicity assay Tü109 supernatant was demonstrated on a panel of 424 HLA-ABC, -DR, -DQ, -MT typed normal Caucasian blood donors to define an epitope on HLA-B locus molecules in great association with the supertypic specificity Bw4. Reactivity of supernatant showed MHC linked inheritance of the Tü109 determinant and discriminated the HLA-Bw4/Bw6 associated HLA-B locus split antigens. Weak or lack of binding on lymphocytes from some HLA-Bw4 heterozygous individuals, particularly typing for HLA-Bw44, appeared to be due to qualitative and/or quantitative variations of HLA-B locus molecules on the cell surface. With Tü109 ascites fluid, however, extra-reactivity on all HLA-Bw6+ cells was demonstrated. Preferential binding of supernatant to HLA-Bw4, but reactivity of ascites fluid with HLA-Bw6+ molecules in addition, was furthermore confirmed by IEF analysis of antigens immunoprecipitated with Tü109 from cell lysates. Thus the antibody may help to analyze the evolutionary relationship of the diallelic specificities Bw4 and Bw6.

Antibodies, Monoclonal↗
PubMed1984

The new HLA-DRw6- and 8- associated HLA-Dw HAG specificity defined by homozygous typing cell 9W 1802. Analysis with primed lymphocyte typing clones.

Intrafamilial primary mixed lymphocyte culture (MLC) typing established that an HLA-A, B, C homozygous, DP heterozygous donor HAG was homozygous for HLA-Dw and behaved as a homozygous typing cell (HTC). Both haplotypes of the HTC were HLA-DR identical, but could not be assigned a clear DR specificity, giving reactions with sera containing antibodies against DRw6, DRw8 and TA10. MLC checkerboard studies failed to assign the HTC HAG specificity to any established or provisional cluster, suggesting that it defined a new Dw specificity. Primed lymphocyte typing (PLT) clones derived from intra-familial priming against either HAG haplotype displayed heterogeneous reactivity patterns. One clone was restimulated only by family members and unrelated donors positive for Dw HAG. Other clones were restimulated by determinants associated with either Dw8 or Dw6. Blocking of stimulation with monoclonal antibodies against different class II molecules suggested that while stimulatory determinants associated with Dw HAG and Dw8 were classifiable as HLA-D related, those associated with Dw6 were of a DP-like nature.

Antibodies, Monoclonal↗
PubMed1982

[Bone marrow transplantation in patients with leukemia].

Between October 1979 and March 1982, bone marrow transplantations were performed by the Tübingen Group for BMT on 19 patients with acute leukemia in remission and on one patient with chronic myelocytic leukemia in chronic phase. The conditioning regimen consisted of 2 x 60 mg cyclophosphamide/kg and 10 Gy whole-body irradiation with the linear accelerator. The lung dose was limited by shielding to 8 Gy. In 15 patients, the bone marrow cell suspension of the donor was preincubated with antihuman T-cell globulin (AHTCG) for prophylaxis of graft-versus-host disease (GVHD). All patients showed prompt engraftment of donor cells with good hemopoietic function and complete chimerism. Under reverse isolation in sterile units, no severe bacterial or fungal infections were seen in the phase of bone marrow aplasia. Twelve in twenty patients survived between 25 and 900 days. A severe GVHD was seen only in two patients - one after preincubation with AHTCG. One patient died from relapse of his leukemia, another patient had a testicular relapse which was treated with local radiotherapy. Major problems were seen with chronic GVHD (six patients) and infectious complications, most importantly interstitial pneumonia, in the late post-transplant period.

Acute Disease↗
PubMed1982

Aminolevulinate dehydratase (E.C. 4.2.1.24): linkage analysis.

Linkage data on aminolevulinate dehydratase (ALADH, E.C. 4.2.1.24) and a series of other human genetic markers are presented. One hundred and two families (25 of them being informative) from southwestern Germany were tested. Close linkage (theta = 0.05) between ALADH and the following markers could be excluded: Rh, PGM1, Fy, ACP1, MNSs, HLA, Bf, GLO, PGM3, Jk, Pi, PGP, K, GPT. There is some evidence of possible linkage with HPA.

Electrophoresis, Starch Gel↗
PubMed1982

Monoclonal antibody (Tü48) defining alloantigenic class I determinants specific for HLA-Bw4 and HLA-Aw23,-Aw24 as well as -Aw32.

A monoclonal antibody (Tü48) was prepared against human lymphocytes. Immunochemical analysis indicated that Tü48 binds to a fraction of HLA molecules. Tü48 was tested on 398 HLA-A,B,C/DR-typed normal blood donors in the microcytotoxicity assay and was found to detect a "supertypic" determinant on molecules bearing the HLA specificities -Bw4, Aw23(9), -Aw24(9), and -Aw32. Among the HLA-Bw4-positive individuals, negative or weak reactivity of Tü48 was found with about one-fourth of normal donors heterozygous for the HLA antigen Bw44. This points to the possibility that the antigenic determinant detected by Tü48 is not expressed on all HLA-molecules carring the HLA-Bw4 specificity on the cell surface. Alternatively, weak or no expression of the Tü48 antigenic determinant on some HLA-Bw44 bearing molecules might be explained by the existence of molecular variants of glycoproteins with this HLA specificity. The concept of "supertypic" HLA specificities is discussed with regard to the expression of a monoclonal antibody-defined epitope on both certain HLA-A and B molecules.

Animals↗
PubMed1980

Data on linkage relations between GLO and 21-hydroxylase.

Linkage between GLO and 21-hydroxylase was investigated in 11 families with 24 children. Positive lod score values with a maximum of +1.618 at theta = 0.05 indicate close linkage between these marker loci.

Adrenal Hyperplasia, Congenital↗
PubMed1980

[HLA antigens in uveitis anterior (author's transl)].

From January 1977 to May 1978, 101 patients with anterior uveitis were typed for HLA antigens. All came from the southwestern area of Germany, and 75 of these patients exhibited no signs of associated systemic disease (21 under 20 years of age, 54 over 20). In this group of 75 patients without associated systemic disease, a B27 frequency of 33.3% (8.9% in a random population) and a Cwl frequency of 16.0% (5.8% in a random population) was found as well as a B27-Cwl linkage of 13.3% (1.9% in a random population). A significant association with HLA antigens was found in (1) the adult form of anterior uveitis (disease onset over 20 years of age), namely, B27 antigen 38.9%, Cwl antigen 18.5%, B27-Cwl linkage 16.7%; and (2) male patients with anterior uveitis but without associated systemic disease, namely, B27 antigen 46.2%, Cwl antigen 17.9%, and B27-Cwl linkage 17.9%. No significant association with HLA antigens was found in (1) the juvenile form of anterior uveitis (disease onset under 20 years of age) and (2) female patients with anterior uveitis but without associated systemic disease.

Adolescent↗
PubMed1979

Segregation of HLA--D specificities with the HLA--B12 allotype.

In a search for bone marrow donors and HLA-D homozygous cells, MLC family studies were performed. Results of the experiments, in which the Seventh Workshop cells 7W569-KR and 7W568-WE were found, are presented here. Within the KR and the WE families two identified and two non-identified HLA-B12 allotype that both families carry.

Alleles↗
PubMed1978

Mapping of the linkage group GLO--Bf--HLA-B,C,A--PGM3. 1. Recombination frequencies.

This study confirms close linkage for the GLO--Bf--HLA-B,C,A complex, and proves linkage between the MHC loci and PGM3. For GLO--PGM3 and Bf--PGM3, respectively, loose linkage seems to be likely, and close linkage can be excluded. Our mapping data on chromosome 6 favor the hypothesis that the PGM3 locus is situated on the HLA-A side of the MHC complex. Yet fine-structure mapping should be confirmed only by segregation analyses in crossover families by testing simultaneously all of the relevant marker loci within uniform family material.

Chromosome Mapping↗
PubMed1978

Mapping of the linkage group GLO--Bf--HLA-B,C,A--PGM3. 2. Segregation analysis.

Segregation analysis of informative families for chromosome 6 markers confirmed the map order GLO--Bf--HLA-(B, C)--HLA-A, and, surprisingly, implies that PGM3 is more probably located on the HLA-A than on the HLA-B side of the linkage group. Therefore the map position of PGM3 should be reconsidered, i.e., more informative families should be tested for all the relevant marker loci available.

Chromosome Mapping↗
PubMed1978

Classification of leukemic cells by Ia alloantigens and complement receptors. Surface probes for cell differentiation.

Of particular interest in the study of cell membrane markers of leukemic cells were cytotoxic and immunofluorescent results obtained in comparing Ia alloantigen and complement receptor (CR) expression on normal leukocytic and leukemic cell types. Using discontinuous Ficoll gradients, Ia alloantigens were found on varying numbers of leukemic myelo- and lymphoblasts, and on the early stages of normal melocytes. Ia alloantigens, however, were not detectable on mature polymorphonuclear cells. This establishes human Ia alloantigens as cell surface differentiation markers. The appearance of complement receptors was observed later than that of Ia alloantigens. CR1 (EAC1-4b) showed up later in the differentiation than CR2 (EAC1-3d). Thus, an immunological discrimination between AML blasts and CML blast crisis blasts appears to be possible: AML blasts are mostly Ia-positive but CR-negative, whilst CML blast crisis cells are only 20-30% Ia-positive and carry complement receptors in at least equal amounts. The AML blast cell would appear as the less-differentiated cell type when compared to the CML blast crisis cells. The picture, however, remains complex since in CML blast crisis at least three different types of blast cells can be identified: an Ia-positive and an Ia-negative myeloid blast as well as an Ia-positive lymphoid blast. The quantitative composition of these three elements within the myeloid differentiation profile can vary somewhat from patient to patient. Furthermore, these studies revealed a disturbed differentiation of leukemic cell types.

B-Lymphocytes↗