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Biomedical subjects

F Sasaki

Publications and source records attributed to F Sasaki.

At least 199 records · Page 11Linked to original sources
PubMed1988

[Establishment of the human hepatocellular carcinoma cell line and its characteristics].

c-Hc-4 has been established and maintained for more than seven years. The hepatocellular carcinoma originated in 45-year old man with liver cirrhosis. The cell grew in vitro forming a sheet of monolayered cells and firmly attaching to the inner surface of cultured flasks. Morphologically they showed epithelial-like pattern. The doubling time was about 20 hours. Their modal chromosome number was 58. Serial heterologous transplantation in nude mice was successful. The histological finding was almost the same patterns as those in the primary tumor. The cultured cells produced alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA).

Animals↗
PubMed1988

[Study on the chemosensitivity of liver cell carcinoma by subrenal capsule assay].

Chemosensitivity of liver cell carcinoma was studied by subrenal capsule assay. The method of assay was based on Bogden's one, but the antitumor activity was evaluated by tumor growth inhibition rate (TG-IR). The anticancer agent with more than 50% TG-IR was judged as positive in the chemosensitivity test. Of 3 human hepatoma cell lines transplanted in the subcutaneous space of nude mice, all of 3 were evaluable. The positive rates of ADR, MMC, CDDP, 5-FU and CPA were 66.7%, 100%, 66.7%, 100% and 0%, respectively. Of 24 patients who provided fresh tumor specimens for the assay, 12 (50%) were evaluable. The positive rates of ADR, MMC, CDDP, 5-FU and CPA were 25%, 16.7%, 16.7%, 33.3% and 8.3%, respectively. Our study suggested that 5-FU, MMC and ADR were comparatively active against the hepatoma cell, CDDP was less active than these 3 agents, CPA was inactive. These results seem to justify the use of current anticancer agents against hepatic cell carcinoma and indicate the usefulness of SRC assay for selecting chemotherapeutic agents against liver cell carcinoma.

Animals↗
PubMed1988

[A study of transfer of cefbuperazone into postoperative exudates in patients with cancer mastectomy or thyroidectomy].

Cefbuperazone (CBPZ) at a dose of 2 g was administered postoperatively by intravenous drip infusion to 9 patients subjected to radical mastectomy and 10 others subjected to thyroidectomy then levels of CBPZ in postoperative exudates were measured and its prophylactic effect on postoperative infections was determined. 1. Serum CBPZ levels in the patients after cancer mastectomy and thyroidectomy on postoperative day 1 were similar to those in healthy adults. 2. Levels of CBPZ in the postoperative exudates in patients subjected to cancer mastectomy reached a mean peak value of 66.3 micrograms/ml (range: 26.0-99.6 micrograms/ml) in 0-3 hours after administration, and the mean CBPZ level at 6 hours after administration was 33.3 micrograms/ml (range: 19.1-54.1 micrograms/ml). 3. As compared to the cases of cancer mastectomy, levels of CBPZ in postoperative exudates in patients subjected to thyroidectomy varied considerably from a patient to another: a mean peak level of 76.4 micrograms/ml (range: 31.3-128 micrograms/ml) appeared in 0 to 6 hours after administration. 4. There was no correlation between CBPZ levels in the exudate and hemoglobin levels or hematocrit values. 5. Likely because of the CBPZ administration at 4 g/day for 2 to 6 days postoperatively to 19 patients, postoperative infection was absent and no side effect attributable to this drug occurred in any of the patients. Because levels of CBPZ in postoperative exudates in patients subjected to cancer mastectomy or thyroidectomy were greater than MICs for principal Gram-positive and Gram-negative bacteria, it is likely that this drug is a useful agent for prophylaxis against postoperative infections in patients undergoing cancer mastectomy or thyroidectomy.

Adult↗
PubMed1987

Application of computed tomography for diagnosis of alveolar bony defects.

This study was performed to evaluate the usefulness of computed tomography (CT) in analyzing the three-dimensional structure of alveolar bony defects. The result showed that differences between CT film and actual readings from the cusp ridge to the bone crest were negligible, and the reading on CT film was underestimated approximately 1 mm at the distance from the cusp ridge to the bottom of the bony defect. By reading a series of CT films, one can easily evaluate the type of bony defects. Therefore, application of CT maybe useful for analyzing three-dimensional structure and diagnosis of the alveolar bony defects.

Adult↗
PubMed1986

Regional specificity of anuran larval skin during metamorphosis: dermal specificity in development and histolysis of recombined skin grafts.

Skins from back and tail were dissected from tadpoles of Rana japonica prior to resorption of the tail and separated into epidermis and dermis by treatment with neutral protease. Homotypically and heterotypically recombined skins were constructed from the separated epidermis and dermis and transplanted into the tail of the original tadpole. Skin grafts using dermis from tail region degenerated simultaneously with resorption of the tail. However, skin grafts containing dermis from back region survived on the posterior part of the juvenile frog beyond metamorphosis. Furthermore, all epidermis underlaid with dermis from back region formed secretory glands and became flattened epithelia characteristic of adult back skin, regardless of region from which the epidermis came. Even when epidermis isolated from tail skin was cultured inside a back skin graft, the tail epidermis survived forming an epithelial cyst and developed secretory glands. These results suggest that regional specificities of anuran larval skin, i.e., development of back skin and even histolysis of tail skin, are determined by regionally specific dermis. The results also suggest that some of epidermal cells of tail skin are able to differentiate into epithelial cells similar to back skin of the adult under the influence of back dermis.

Animals↗
PubMed1986

Normal and malignant neural cells: a comprehensive survey of human and murine nervous system markers.

Tumor-associated neural markers are finding increased application in diagnostic histopathology and in the development of brain tumor therapy. The major cell-type-specific markers and monoclonal antibodies that identify murine and human neural cells are reviewed in this study. Monoclonal antibodies, raised against fetal and adult neural tissue, neuroectodermal tumor tissue, or cell line immunogens which recognize epitopes on brain tumors are comprehensively described including antigens common to the nervous, hematopoietic, and immune systems. The clinical application of neural cell markers and monoclonal antibodies for the diagnosis, localization, and treatment of neuroectodermal tumors is reviewed.

Animals↗
PubMed1986

Effects of a thromboxane synthetase inhibitor (OKY-046) and a lipoxygenase inhibitor (AA-861) on bronchial responsiveness to acetylcholine in asthmatic subjects.

The effect of a selective thromboxane synthetase inhibitor, OKY-046, and a selective 5-lipoxygenase inhibitor, AA-861, on bronchial responsiveness to acetylcholine was studied in 23 asthmatic subjects. The provocative concentration of acetylcholine producing a 20% fall in forced expiratory volume in one second (PC20 FEV1) was measured before and after oral administration of OKY-046 (3000 mg over four days) and AA-861 (1100 mg over four days) and inhalation of OKY-046 (30 mg) in 10, 10, and nine asthmatic subjects respectively. Baseline values of FEV1 and forced vital capacity (FVC) were not altered by oral OKY-046, oral AA-861, or inhaled OKY-046. The geometric mean value of PC20 FEV1 increased significantly from 0.55 to 2.24 mg/ml after oral OKY-046, but was unchanged after inhalation of OKY-046 and after oral administration of AA-861. These results suggest that thromboxane A2 may play a part in bronchial hyperresponsiveness to acetylcholine.

Acetylcholine↗
PubMed1986

Roles of the arcuate nucleus and ovary in the maturation of growth hormone, prolactin, and nongranulated cells in the mouse adenohypophysis during postnatal development: a stereological morphometric study by electron microscopy.

With the use of monosodium L-glutamate (MSG) treatment and ovariectomy, we studied how the arcuate nucleus and ovary are involved in the maturation of GH, PRL, and nongranulated cells in the anterior pituitary lobe during postnatal development. From day 1 to day 7 female pups were injected sc daily with MSG (2 mg/g BW) (MSG mice) or distilled water (control mice). Just before puberty (at 30 days of age) some control and MSG mice were ovariectomized (OVX) (control + OVX mice and MSG + OVX mice, respectively). All animals were killed at 60-70 days of age by decapitation. Populations of GH, PRL, and nongranulated cells in the anterior pituitary lobe were calculated by applying morphometric procedures. Percentages of GH, PRL, and nongranulated cells in MSG mice were not different from those of control mice. In control + OVX mice the percentage of GH cells was greater than that of control mice, while PRL cells were markedly decreased. On the other hand, in MSG + OVX mice the percentage of PRL cells was less than that of MSG mice, while GH cells showed no difference; nongranulated cells were increased. The number of parenchymal cells in the anterior pituitary lobe in MSG mice was approximately two thirds that of control mice. The parenchymal cells in control + OVX mice were the same in number as those in control mice, while those in MSG + OVX mice were approximately half the number of the parenchymal cells in MSG mice. Numbers of GH and PRL cells in MSG mice were half those of control mice. There was no difference in the number of nongranulated cells between MSG and control mice. GH cells in control + OVX mice were more abundant than those of control mice, while PRL cells were fewer. On the other hand, both GH and PRL cells in MSG + OVX mice were fewer when compared with those of MSG mice. The numbers of nongranulated cells in most groups showed no significant differences among each other. All of these data taken from control mice were the same as those taken from normal mice. These results suggest that the arcuate nucleus in the hypothalamus stimulates the differentiation of GH and PRL cells during postnatal life, and that the ovary stimulates the differentiation of PRL cells through a direct effect on the anterior pituitary lobe but inhibits that of GH cells via the arcuate nucleus in the hypothalamus, while the differentiation of nongranulated cells is little affected.

Animals↗