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Biomedical subjects

F Sasaki

Publications and source records attributed to F Sasaki.

At least 181 records · Page 10Linked to original sources
PubMed1989

Kidney-preserving radical tumor resection in advanced neuroblastoma.

The possibility of kidney-preserving radical tumor resection in advanced neuroblastoma was evaluated. Eighteen patients with stage III and IV adrenal neuroblastoma underwent delayed primary surgery after chemotherapy consisting of high doses of cyclophosphamide (1,250 mg/m2). Radical tumor resection with nephrectomy was performed in 13 patients (group A) and kidney-preserving radical tumor resection was performed in six patients (group B). Macroscopic findings were evaluated in all patients; angiographic and microscopic findings were evaluated in group A patients and tumor recurrence in group B patients. Macroscopically, the tumors were observed within the tumor capsule (C1) in five, outside the tumor capsule but not beyond the midline (C2) in seven, and beyond the midline (C3) in six. In group A, under microscopic examination, the tumor invasion into the renal parenchyma was observed in only three patients among C2 and C3 cases. Tumor vessels from the capsular and/or perforating arteries plus from the intrarenal arteries could be visualized in all patients, three of whom had tumor invasion into the renal parenchyma. In group B, no tumor recurrence around the kidney occurred, and two patients have survived for greater than 2 years. The kidney-preserving radical tumor resection is recommended in surgical treatment of advanced neuroblastoma following preoperative chemotherapy, and is predictable preoperatively by angiography.

Adrenal Gland Neoplasms↗
PubMed1989

Classifications of somatotropes, lactotropes and corticotropes in the mouse adenohypophysis with immunohistochemistry.

Somatotropes, lactotropes and corticotropes of adult male mice were identified with immunohistochemistry in the adenohypophysis fixed by OsO4 alone. Somatotropes were classified into type I somatotropes that contain large (350 nm in diameter) round secretory granules and type II somatotropes that contain small (100-200 nm in diameter) round secretory granules. Most somatotropes were type I somatotropes. Lactotropes were also classified into type I lactotropes that contain irregularly shaped secretory granules and type II lactotropes containing small (100-200 nm in diameter) round secretory granules. Corticotropes are irregular stellate or slender cells with little cytoplasm. They contain round solid secretory granules in various densities along the cell periphery. Most of these are low-density granules (200-300 nm in diameter) and a few are high-density granules (200-250 nm in diameter). These data were compared with the classical data of mouse adenohypophysial cells that were fixed in OsO4 alone and identified only by conventional electron microscopy.

Adrenocorticotropic Hormone↗
PubMed1989

[Clinical study of intrahepatic arterial infusion of unresectable hepatoblastoma and hepatocarcinoma in children].

Six cases of unresectable hepatic cancer in infant were treated with intra-arterial infusion therapy. The histological types were hepatoblastoma and hepatocellular carcinoma, 3 cases respectively. The clinical stages were 1 recurrent case in I, 1 in IIIA, 2 in IIIB and 2 in IV. Seldinger method and cannulation at laparotomy were employed in 4 cases and 2 cases, respectively. In the eldest case, a catheter with dual lumen reservoir developed in our department was inserted, making it possible to infuse drugs into hepatic artery and cutting off hepatic arterial blood flow temporarily. The anticancer drug used was ADM, CDDP, 5-FU, THP-ADM, and MMC; antiAFP-anticancer drug conjugate missile therapy was employed in 4 cases. According to image diagnosis, reduction or necrosis of tumor was observed in 5 cases. In all cases, AFP scores decreased. In 5 dead cases, 4 cases died of tumor enlargement (average survival time 16.3 months); 1 case died of DIC during chemotherapy. The other case could eventually undergo complete resection and is now alive. Intra-arterial infusion therapy seemed to be useful for patients of infant unresectable hepatic cancer.

Adolescent↗
PubMed1989

[Development of a new therapeutic method in hepatocellular carcinoma using a culture system--the intensifying effect of human epidermal growth factor (hEGF) on the antitumor action of antitumor agents].

The culture system of hepatocellular carcinoma (HCC) has been established and the effect of hepatotrophic factors on these cell lines have been studied. During this study the effect of the human epidermal growth factor intensifying anti-tumor action of anti-tumor agents will be evaluated. HCC cell lines (C-HC-4, C-HC-20) show logarithmic growth in the serum-free medium. These growth curves after administration of 1 x 10(-12- -8) M of hEGF were measured, and the existence of the hEGF receptors was evaluated using the ABC method. The growth curve of HC-4 transplanted into nude mice was measured in 4 groups; the control group, the hEGF group, the gamma Interferon (gamma IFN) group, and the hEGF + gamma IFN group. The hEGF enhanced the cell growth of C-HC-4 and C-HC-20, and EGF receptors were confirmed immunohistologically. In the hEGF+ gamma IFN group, growth inhibition seems to be most important. The hEGF may intensify the anti-tumor action of the anti-tumor agents.

Animals↗
PubMed1989

[Alveolar hydatid disease of the liver in children--radiographic findings].

The alveolar hydatid disease of the liver is uncommon in childhood. Six were less than 15 years old in our hospital. Among the first three patients, two had hepatomegaly, and the third patients had liver dysfunction. The latest three patients were detected by serological test and ultrasonographic examination in the mass screening when the lesions were too small to produce symptoms. Then CT and angiography were done for more precise examination. For the good prognosis, an early diagnosis and complete removal of the lesion are indispensable.

Adolescent↗
PubMed1988

Morphological changes in the oral (buccopharyngeal) membrane in urodelan embryos: development of the mouth opening.

The ultrastructure of the oral (buccopharyngeal) membrane in the embryo of the urodelan, Hynobius tokyoensis, was examined by transmission (TEM) and scanning electron microscopy (SEM). The oral membrane consists of the stomodeal ectoderm and foregut endoderm, and is three to five cell layers thick at stage 24. The oral membrane gradually thickens as development proceeds. The stomodeal collar, derived from the ectoderm, is folded inward along the foregut endoderm. Tooth germs are formed partly by cells of the stomodeal collar and partly by mesenchymal cells and calcification takes place before hatching. Secretory granules, which are markers of epithelial differentiation, appear in some cells of the foregut endoderm. Within the oral membrane, the cells of the stomodeal collar become the basal cells, and the endodermal cells of the foregut become the apical cells of the future oral epithelium. Gaps are formed by the epithelial differentiation of the endodermal cells of the foregut in the oral membrane. The gaps connect with each other, with the stomodeum, and with the foregut. As a result of these events, the mouth opens at stage 43, just after hatching.

Animals↗
PubMed1988

Cytochemical studies of hydrogen peroxide production in the tadpole tail of Rana japonica during metamorphic climax.

The degeneration of tadpole tail tissue was investigated cytochemically by localizing the sites of hydrogen peroxide production. A cerium perhydroxide precipitation method was used. No reaction product was found in resting macrophages and intact muscle fibres during premetamorphosis. In the metamorphosis phase, extensive cerium precipitates were visualized on the outer surface of the plasma membrane of phagocytotic macrophages, fibroblasts, neutrophils, epidermal cells, muscle fibres, notochordal cells, nerve cells and capillary endothelial cells. The reaction products were localized on those parts of the plasma membranes of the macrophages that were in contact with those of adjoining cells. When catalase were added, the amount of deposits decreased. alpha-Tocopherol and indomethacin, but not dexamethasone, significantly inhibited the formation of the reaction products. These findings are taken to indicate that active oxygen is produced on the plasma membrane of activated macrophages and may play a role in the degeneration of the tail tissue.

Animals↗
PubMed1988

Sphincteromyectomy and sphincteroplasty in chronic constipation with megarectum.

An operative procedure for patients with chronic constipation with megarectum and its results is presented. Eleven cases of idiopathic chronic constipation showing megarectum on barium enema underwent this operation. Bowel habits of operated patients were evaluated at 1 to 5 years after the operation. Complete cure was obtained in eight cases. Three cases were improved but needed occasional suppositories. This procedure, sphincteromyectomy and sphincteroplasty, may be an operation of choice for chronic constipation with megarectum, when nonoperative methods fail.

Adolescent↗
PubMed1988

Correlation of spatial differences in concentrations of prolactin and growth hormone cells with vascular pattern in the female mouse adenohypophysis.

Adult female mice of the DDY/S strain were used to study the distribution of PRL or GH cells and the vasculature of the anterior pituitary lobe. Electron microscopy was used to quantify PRL or GH cells in horizontal sections. Most parenchymal cells were either PRL or GH cells, and both types of cells were present in all regions. The densities of PRL cells in the rostral and caudal areas were significantly greater than that of GH cells. The density of GH cells was greater in the anterolateral wings. Thus, the spatial differences in concentrations of PRL and GH cells were reversed. The vasculature was studied with scanning electron microscopy of vascular casts and with stereoscopy of pituitary glands injected with India ink. The adenohypophysis was supplied by long and short portal vessels. The long portal vessels originated from the primary capillary plexus on the median eminence and the upper portion of the pituitary stalk, and they supplied rostral regions of the adenohypophysis. Most of the short portal vessels connected caudal areas of the anterior lobe with the posterior lobe, crossing the surface of the intermediate lobe. The blood in the short portal vessels may flow from the posterior lobe toward the anterior lobe. Thus, within the rostral and caudal areas, which are supplied by long and short portal vessels, respectively, PRL cells predominated; the anterolateral wings where GH cells predominated were far from these regions. These data suggest that the anatomical pattern of the blood supply may account in part for the spatial distribution of PRL and GH cells.

Animals↗
PubMed1988

Sex difference in prolactin and growth hormone cells in mouse adenohypophysis: stereological, morphometric, and immunohistochemical studies by light and electron microscopy.

PRL and GH cells in the adenohypophysis of adult mice were studied with immunohistochemistry and stereological morphometry by light and electron microscopy. We compared findings for males and females. In female mice, most PRL-immunoreactive cells (43% of all parenchymal cells) and most GH-immunoreactive cells (33% of all parenchymal cells) were classical PRL cells (type I PRL cells) or GH cells (type I GH cells), respectively. Besides these classical types of cells, we found PRL- and GH-immunoreactive (type II PRL and GH) cells that contained small (approximately 100-200 nm in diameter) and round secretory granules. However, in female mice type II PRL or GH cells accounted for less than 3% of all parenchymal cells, as was also true for type II GH cells in the males. The percentages of type I and type II PRL cells in male mice were about the same (approximately 10% of all parenchymal cells). Only a few mammosomatotropes (0-0.6% of all parenchymal cells) were found in the mouse adenohypophysis of either sex. All mammosomatotropes were type II cells; none were PRL or GH cells of the classical type. This immunohistochemical study confirmed our previous findings by conventional electron microscopy that there are marked sex differences in the proportions of PRL and GH cells in the adult mouse adenohypophysis; PRL cells are more abundant in the female mice, and GH cells are more abundant in the males.

Animals↗
PubMed1988

Changes with age in the number and size of anterior pituitary cells in female mice from suckling to adulthood.

Changes with age in the number and size of anterior pituitary cells in female mice were calculated during their postnatal development by using a stereological morphometric study with electron microscopy. The number of parenchymal cells increased in mice from 20 to 30 days of age, and did not change around puberty, after which the number increased to the adult level. The number of somatotrophs increased with age in almost the same manner as the parenchymal cells. The number of lactotrophs increased with age and were significantly different each time they were measured. The number of non-granulated cells did not increase in mice from 20 days of age to adulthood; at 20 days of age, the number was at the same level as in the adult mice. The other types of cells increased by only a small number. The sizes of all types of cells increased during postnatal life. Somatotrophs and lactotrophs became the same size as in adults by the onset of puberty. Non-granulated cells and other types of cells reached adult size at 5 days after puberty. Lactotrophs and somatotrophs had adult ultrastructural features on the day of puberty. Sizes and ultrastructural features of anterior pituitary cells reached adult levels on the day of puberty, but their numbers were still fewer than in adult mice. The increase in the volume of the anterior pituitary with age arose mostly from an increase in the number and the size of somatotrophs and lactotrophs before puberty, increases in the size of somatotrophs and the number of lactotrophs around puberty, and an increase in the number of both types of cells after puberty.

Aging↗