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Biomedical subjects

F Potet

Publications and source records attributed to F Potet.

At least 163 records · Page 9Linked to original sources
PubMed1984

Collagenous colitis. Ultrastructural study and collagen immunotyping of four cases.

We studied four cases of collagenous colitis (CC), which is characterized by thickening of the subepithelial collagenous layer of the colorectal mucosa and is associated with chronic watery diarrhea. Eighteen cases of CC have been previously reported in the literature, to our knowledge. In our four patients, ultrastructural study and collagen immunotyping demonstrated that the basement membrane over the thickened collagen was normal. We found no similar collagenous thickening in a series of 96 biopsy specimens of chronic inflammatory bowel diseases, but collagenous thickening was noted in 17 (25%) of 68 colonic metaplastic polyps. The cause of CC is unknown. The pericryptal fibroblastic sheath of the colonic mucosa has a probable role in the development of CC and metaplastic polyps. The role of the numerous mast cells present in our four cases of CC is currently unknown.

Adult↗
PubMed1983

Relationship between liver histopathological changes and HBsAg in 111 patients treated by long-term hemodialysis.

We studied liver biopsies performed between January 1972 and June 1980 in 111 patients receiving regular dialysis treatment. Biopsies were performed either because of suspected liver disease (61 patients) or routinely during abdominal surgery or kidney transplantation (50 patients). Repeat biopsies were done in 14 cases. Hepatitis B virus markers, assayed every 3 months during the observation period, were detected at some time in 71 patients (64%); 51 remained persistently positive. Histological examination showed normal liver in 39 cases, lobular hepatitis in 15, chronic persistent hepatitis in 36 and chronic active hepatitis in 21. All patients with chronic active hepatitis were chronic HBsAg carriers, and repeated biopsies showed aggravation only in these patients. The course was remarkably asymptomatic, with lesions leading to fibrosis despite the lack of histopathological patterns of severe necrosis and/or inflammation, which were conspicuously absent in this series.

Biopsy↗
PubMed1983

[Primary histiocytic lymphoma of the liver. Study of 2 cases and review of the literature].

The authors report two cases of primary histiocytic malignant lymphoma of the liver successfully treated by right hepatectomy. The first case, was free of secondary spread with a follow-up of ten years, although he received no adjuvant therapy. The second patient developed skin, bone and brain metastases, which disappeared after chemotherapy and radiotherapy. He has been free of recurrence for four years. Such primary liver tumors are very rare and are worth knowing because of the efficiency of treatment and of their rather good prognosis. Evolution is different from that observed in histiocytic malignant lymphomas developed in other sites. It is suggested that, in the liver, this tumor might originate and grow from Kupffer's cells. This histologic origin might explain the particular clinical and evolutive features of primary histiocytic malignant lymphomas of the liver.

Adult↗
PubMed1983

[Value of liver puncture-biopsy for diagnosis, classification and treatment of chronic hepatitis].

In the assessment of chronic hepatitis, the liver biopsy serves the following purposes: 1) to confirm the fact that it is chronic. 2) to evaluate its degree of activity. 3) to check if there is cirrhosis or not. 4) to seek morphological proofs for the presence of viral, drug-induced or other aetiological agents. 5) to follow the evolution (in the natural course or during treatment) of the disease by comparing repeated liver biopsies. The initial classification of chronic hepatitis--proposed in 1968--has been reviewed by numerous authors; at the moment, it seems necessary, in order to correlate clinical and histological results better, to emphasize two important facts: 1) the spreading of liver necrosis if there is chronic active hepatitis. 2) the presence--or not--of cirrhosis. Some morphological arguments could be useful in order to diagnose the aetiology of chronic hepatitis. Empirical and specific immunological methods have become available for demonstrating HBsAg. If a chronic active hepatitis with multisystem involvement and/or abnormal serum antibodies is diagnosed, corticosteroid therapy is indicated. In others cases, especially those with virus B disease, the treatment differs according to medical teams.

Biopsy↗
PubMed1982

Characterization of a newly established human gastric cancer cell line HGT-1 bearing histamine H2-receptors.

A human gastric adenocarcinoma cell line, HGT-1, was established in vitro from the primary tumor of a 60-year-old patient. Histological examination of the tumor revealed a poorly differentiated adenocarcinoma. Primary tumor cells were cloned in soft agarose and gave rise to tumor colonies. The procedures enabling us to form a continuous cell line from the agarose colonies are described. The cultured cells grew as monolayers of closely apposed polygonal cells with a population-doubling time of 19.48 +/- 1.20 (S.E.) hr during exponential growth at passage 59. They had an epithelial morphology. Ultrastructural studies revealed the presence of microvilli and tight junctions. The HGT-1 cell line is tumorigenic in nude mice and has a hyperdiploid karyotype with a modal number of 57 chromosomes. It exhibits numerous marker chromosomes. These human gastric epithelial cells do not secrete mucus or carcinoembryonic antigen. They exhibit functional histamine H2-receptors mediating cellular cyclic adenosine 3':5'-monophosphate production and adenylate cyclase activation. In conclusion, the use of a soft-agarose clonogenic assay permitted us to develop a cancer cell line without the problems of fibroblastic cell contamination. The existence of histamine H2-receptors on gastric HGT-1 cells stresses the importance of this line as a model for studies of regulatory mechanisms involved in gastric secretion.

Adenylyl Cyclases↗
PubMed1981

State of hepatitis B virus DNA in hepatocytes of patients with hepatitis B surface antigen-positive and -negative liver diseases.

Using the Southern blot technique and cloned hepatitis B virus (HBV) DNA as a probe, we studied the state of HBV DNA in the liver of 13 patients with hepatocellular carcinoma, 17 patients with chronic hepatitis, and 2 patients with acute hepatitis. The hybridization results were compared with the serological and immunohistological data. Integration of HBV DNA in cellular DNA of the liver from patients with hepatocellular carcinoma was demonstrated. In two patients from which tumorous and nontumorous liver tissue samples were available the integration patterns were different. In one patient with hepatitis B e antigen (HBeAg)-positive early hepatocellular carcinoma, free viral DNA was present in the liver. In some patients with HBeAg-negative chronic hepatitis, without tumor, integration of HBV DNA in cellular DNA was also demonstrated. This suggests that HBV is not the only factor involved in the development of a tumor. In patients with HBeAg-positive chronic hepatitis, free viral DNA was detected in the liver. In the two acute hepatitis patients analyzed, the restriction endonuclease patterns strongly suggested HBV DNA integration. Therefore, viral DNA integration seems to occur early in infection. Whatever the form of the disease, discrete bands were observed, suggesting the existence of limited and specific integration sites in host cellular DNA. The presence of integrated or free DNA sequences has implications for antiviral therapy. In addition, detection of HBV DNA in the liver is another sensitive viral marker that could be useful for diagnostic purposes.

Carcinoma, Hepatocellular↗
PubMed1981

Hepatocytic PAS-positive diastase-resistance inclusions in the absence of alpha-1-antitrypsin deficiency--high prevalence in alcoholic cirrhosis.

The presence of PAS-positive, diastase-resistant inclusions in the cytoplasm of the hepatocytes is characteristic of alpha-1-antitrypsin deficiency. The purpose of this investigation was to determine whether the presence of these inclusions is a specific feature, permitting the recognition of alpha-1-antitrypsin deficiency in patients with liver disease. We examined the liver specimens from 20 patients suffering from alcoholic cirrhosis with the Pi M phenotype, i.e., in whom alpha-1-antitrypsin deficiency was excluded. In seven of these patients, PAS-positive, diastase-resistant inclusions were seen in the hepatocytes; in two patients, these inclusions contained a material antigenically similar to alpha-1-antitrypsin. These inclusions might represent deposits of glycoproteins poorly excreted by the diseased hepatocytes. It is concluded that, in patients with liver disease, the presence of PAS-positive, diastase-resistant inclusions--even containing alpha-1-antitrypsin--in the cytoplasm of the hepatocytes does not permit the hepatic lesions to be ascribed to alpha-1-antitrypsin deficiency.

Amylases↗
PubMed1981

Superficial oesophageal carcinoma: an oesophageal counterpart of early gastric cancer.

The clinicopathological features of 10 cases of superficial oesophageal carcinoma without lymph node metastasis are reported. Superficial oesophageal carcinoma, an uncommon form of oesophageal cancer, is defined as invasive squamous cell carcinoma confined to the mucosa and submucosa. It includes plaque-like, erosive, papillary and occult types but is not associated with the usual macroscopic features of advanced oesophageal cancer. The main presenting symptom of dysphagia and oesophagitis is a frequently quoted endoscopic diagnosis. Superficial oesophageal carcinoma may be preceded by dysplastic and in situ carcinomatous changes and is probable oesophageal counterpart to early gastric cancer.

Aged↗