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Biomedical subjects

F Potet

Publications and source records attributed to F Potet.

At least 181 records · Page 10Linked to original sources
PubMed1981

Growth and differentiation of human gastrointestinal adenocarcinoma stem cells in soft agarose.

A soft agarose clonogenic assay is presented which has been optimized for the growth of human gastrointestinal adenocarcinomas. Samples from 15 gastric and colonic solid tumors and from 2 noncancerous stomachs (control cultures) were disaggregated by treatment with collagenase at 37 degrees overnight. Colonies appeared 10 to 15 days after plating, with a cloning efficiency between 0 and 0.82%, which was markedly improved by a fibroblastic feeder layer. The results suggest a correlation between cloning efficiency and the degree of differentiation of the initial tumor. Histochemistry, electron microscopy, and a carcinoembryonic antigen immunofluorescence assay showed that the colonies consisted of cells with the same characteristics as those of the original tumor.. This colony formation assay appears to be potentially useful for assessing the stem cell pool of gastrointestinal tumors. It will be valuable for studying their response to chemotherapeutic agents in vitro. This clonogenic assay may also permit the establishment of cancer cell lines.

Adenocarcinoma↗
PubMed1980

[Multiple Carcinomas. Results of 2 813 autopsies (author's transl)].

The presence of multiple primary malignant neoplasms has been proved pathologically in a total of 50 cases recorded during a 5 years period (1970-1974). The 50 patients represented an incidence of 5.4% among the 992 patients proved to have cancer during the same 5 years period. The multiple lesions were diagnosed simultaneously in half of the patients. Twenty six patients had consecutive neoplasms, the average interval between the first and the second cancer was 6.9 years.

Age Factors↗
PubMed1980

Response of rat exocrine pancreas to chronic pulmonary ethanol intoxication.

Rats were intoxicated over a 32-day period by continuous exposure to an ethanol-enriched atmosphere. Under these conditions, body weights increased up to day 26 and blood ethanol levels increased to approximately 2 g/liter. Pancreatic and hepatic histologies were normal. Amylase levels and total pancreatic protein decreased, while lipase levels rose. These changes appear to be independent of the caloric balance of the diet.

Alcoholic Intoxication↗
PubMed1980

Hepatitis B core antigen in hepatocytes of patients with chronic active hepatitis.

Hepatitis B core antigen was demonstrated in the hepatocytes of 28 of 40 patients with chronic active hepatitis in whose sera hepatitis B surface antigen was not detectable by radioimmunoassay. This finding suggests that in these patients, despite hepatitis B surface antigen being undetectable in serum, the liver disease is the consequence of chronic infection with hepatitis B virus.

Adult↗
PubMed1979

Fibrosing alveolitis and hepatitis B surface antigen-associated chronic active hepatitis in a patient with immunoglobulin A deficiency.

Fibrosing alveolitis is described in a 22 year old woman with immunoglobulin A (IgA) deficiency and hepatitis B surface antigen (HBsAg)-associated chronic active hepatitis. At lung biopsy HBsAg was detected by indirect immunofluorescence in the alveolar space but not in the septal fibrosis. We discuss the possible relationships between IgA deficiency on the one hand, and HBsAg-associated lung and liver diseases on the other hand.

Adult↗
PubMed1979

Perhexiline maleate-induced cirrhosis.

The authors report the cases of 2 patients who died from cirrhosis after receiving perhexiline maleate, a drug widely used in Europe for the treatment of angina pectoris. Perhexiline maleate had been ingested for 24 and 28 mo, respectively. Manifestations of cirrhosis included jaundice, hepatic encephalopathy, ascites, and portal hypertension. Associated manifestations of intolerance to perhexiline maleate included peripheral neuropathy in 1 patient and marked weight loss in both. Histologic lesions resembled those observed in patients with alcoholic liver disease. Ultrastructural lesions included numerous enlarged lysosomes containing myeloid figures. Histochemical stains demonstrated increased phospholipid content of the hepatocytes. These findings are consistent with the view that prolonged administration of perhexiline maleate may induce both histologic lesions resembling those of alcoholic liver disease and ultrastructural and histochemical lesions resembling those of phospholipidosis.

Aged↗
PubMed1978

Peliosis hepatis in recipients of renal transplants.

Peliosis hepatis, an uncommon liver lesion characterised by blood-filled cavities bordered by hepatocytic plates, was found in 12 patients three to 17 months after renal transplantation. Hepatomegaly and portal hypertension were present in five of the six patients with major peliosis hepatis, and were absent in the other six with minor hepatic lesions. Alterations of centrilobular vein walls in some of these patients suggest that peliosis hepatis could be the result of a blockade or liver blood outflow at the junctions of sinusoids and centrilobular veins. The cause of these alterations might be azathioprine.

Adolescent↗
PubMed1978

Gastrin cell distribution in normal human stomachs and in patients with Zollinger-Ellison syndrome.

Quantitative distribution of gastrin cells was evaluated in three normal human stomachs and in four stomachs from patients with Zollinger-Ellison syndrome. Cells identified by the immunoperoxidase method were counted along the entire length of five mucosal strips parallel to the axis of the lesser curvature and sampled from the posterior to the anterior walls. The number of cells per unit area (2300 microns2) decreased from the pylorus to the borderline of the gastric body from (mean +/- SEM) 50.9 +/- 12.0 to 24.2 +/- 13.0 and from 29.6 +/- 5.6 to 10.4 +/- 2.6 for control and Zollinger-Ellison syndrome, respectively, with large interindividual variations. From factorial analysis no statistical difference was found between the two groups. It is therefore suggested that the number of gastrin cell in antral mucosa may not be a significant criteria in the diagnosis of Zollinger-Ellison syndrome.

Aged↗