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Biomedical subjects

F Picard

Publications and source records attributed to F Picard.

At least 145 records · Page 8Linked to original sources
PubMed1989

ets-1 and ets-2 proto-oncogene expression in human leukemia cells and cell lines.

c-ets-1 and c-ets-2 are 2 proto-oncogenes known to be possibly involved in some human myelomonocytic leukemias. However, very few studies concern c-ets-1 and c-ets-2 RNA expression in human hematologic malignancies. We have studied 18 leukemic patients, and 10 cell lines for their ets RNA contents. c-ets-1 was strongly expressed in 5 and c-ets-2 in 8 of the 18 patients. All the cell lines expressed both c-ets-1 and c-ets-2 RNAs. This expression was highly variable from one patient to another, and from one cell line to another, regardless of the cellular leukemia subtype. The variability of this expression in patients may reflect differences in the proliferative potential of leukemic cells.

Adult↗
PubMed1988

Peripheral antiglucocorticoid action of RU 486 in man.

In order to evaluate the peripheral antiglucocorticoid activity of RU 486 in man we examined its ability to antagonize the effects of acutely administered glucocorticoids on blood leukocyte counts. The study was performed on eight normal male subjects. They were given 400 mg RU 486 (or placebo) orally at 0730 h and 1 mg dexamethasone (or placebo) orally at 0830 h, using a double-blind, cross-over, latin-square design with a one week interval between each of the four different treatments. Circulating eosinophils, lymphocytes, and neutrophils were counted at 0730 h and 1330 h, and their variations (1330 h counts/0730 h counts x 100) were compared under each treatment. For each cell type, dexamethasone induced variations (eosinophil and lymphocyte drop, neutrophil rise) which were significantly (P less than 0.05) different from those under the three other treatments; these latter treatments (placebo, RU 486, and RU 486 + dexamethasone) were equivalent to each other, inducing no variations of leucocyte counts. These data show that RU 486 inhibits dexamethasone induced leukocyte changes; this simple test provides a useful means to further analyse the peripheral antiglucocorticoid activity of RU 486 in man.

Adult↗
PubMed1987

Extensive T8-positive lymphocytic visceral infiltration in a homosexual man.

Lymphocytic visceral infiltration has recently been noted in association with lymphadenopathy-associated virus infection. A homosexual man, who had clinical and immunologic features of the acquired immune deficiency syndrome (AIDS)-related complex, is described. The patient presented not only with peripheral blood lymphocytosis but also with extensive lymphocytic infiltration involving lungs, lymph nodes, nerves, muscles, and esophagus. Lymphocyte subset immunostaining analysis showed that the lymphocytes were T8-positive. Thirty months after the clinical onset of the disease, no evidence of progression to AIDS was seen. Moreover, clinical improvement was observed, even though the patient did not receive long-term treatment. The clinical history of this patient suggests that lung T8-positive lymphocytic infiltration is associated with an increased risk of infectious episodes such as pneumonia and bronchitis.

AIDS-Related Complex↗
PubMed1987

[De novo interstitial deletion of the long arm of chromosome 2: 46,XXX,del(2)(q14q21), associated with premature craniosynostosis].

A female newborn with premature cranial synostosis and facial dysmorphism including bulging forehead, hypertelorism, downward slanting palpebral fissures, low set ears and a short nose with broad flattened nasal bridge, was shown to have the karyotype 46,XX,del(2)(q14q21). We suggest that the appearance of isolated or associated premature fusion of cranial sutures is related to a structural alteration of the long arm of chromosome 2.

Chromosome Deletion↗
PubMed1986

B cells from leukemic patients are relatively resistant to in-vitro EBV transformation.

Samples of peripheral blood mononuclear cells (PBMC) from normal donors or from leukemic patients were used to obtain Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCL). Whereas the rate of transformation was around 85% with normal donors, it was only around 20% with leukemic patients. However no explanation for this resistance of B cells from leukemic patients to in-vitro EBV transformation could be found. Indeed no correlation exists between this EBV resistance and the age, sex, diagnosis or chemotherapeutic regimen. Furthermore no correlation is apparent between the percentage of B2+ cells, which should have EBV receptors, and the successful EBV transformation.

B-Lymphocytes↗
PubMed1986

46,XX,t(15;21)/47,XX,15p-,+21 mosaicism in a child with Down's syndrome.

We report here the first case of a mosaic Down's syndrome in which both clones are trisomic for chromosome 21, one of them (90%) by a Robertsonian translocation (15;21) appearing de novo, and the other (10%) by an additional chromosome 21. Three hypotheses can explain the appearance of such a mosaic: that of a chimera formed by the fusion of two trisomy 21 zygotes, one of which had a Robertsonian translocation, the other an additional trisomy 21 zygote; that of a fusion between a chromosome 15 and a chromosome 21 in one of the early segmentation blastomeres of a trisomy 21 zygote; the more probable hypothesis of the occurrence of a fission at the break-attachment point of a Robertsonian translocation (15;21) in one of the cells arising from the early postzygotic divisions of a zygote which was a trisomy 21 by Robertsonian translocation (15;21).

Chromosomes, Human, Pair 15↗
PubMed1986

[Value of the cytological examination of the bronchoalveolar lavage fluid in patients with acquired immunodeficiency syndrome and related syndromes].

In AIDS a variety of severe pulmonary disorders may occur. The authors report 110 cases of bronchoalveolar lavage (BAL) in 43 AIDS and 41 ARC. In AIDS P. carinii pneumonia is the major cause of respiratory illness. BAL alone is a safe and valuable tool for diagnosis of P. carinii pneumonia and others opportunistic infections. Moreover, pulmonary hemorrhage diagnosed by the finding of hemosiderin laden macrophages, is very suggestive of broncho-pulmonary Kaposi' sarcoma. Finally, BAL demonstrates a severe depletion of T4 lymphocytes and an increased number of T8 lymphocytes. The T8 lymphocytosis is observed whatever the pulmonary involvement (nonspecific alveolitis, opportunistic infections, Kaposi's sarcoma), and is also found in ARC, and lymphocytosis, open lung biopsy shows a lymphoid interstitial infiltration with respect of the alveolar septa, thus differing from the classical lymphoid interstitial pneumonia described by Carrington. The prognosis of lymphocytosis in ARC remains unknown.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Lymphomas and AIDS].

We report 21 cases of lymphomas associated with AIDS. Sixteen cases are Non Hodgkin's lymphomas of high grade malignancy. Immunoblastic B cell lymphomas are frequent: 11 cases/16, especially with extra nodal localisations. Three cases are Burkitt's lymphoma; 2 cases are large non cleaved cell lymphoma. Five cases of Hodgkin's disease are associated with AIDS related complex syndrome (ARC) showing the interest of lymph node biopsy in such patients. We analyzed 6 cases with lymphoid bone marrow infiltration and discuss the relationship between prelymphomatous states and low grade malignant lymphomas in AIDS or ARC patients.

Acquired Immunodeficiency Syndrome↗
PubMed1985

[Trisomy 20p derived from a maternal pericentric inversion and brachymesophalangy of the index finger].

The article brings to light the very first case of trisomy 20p resulting from a maternal pericentric inversion in a 2 1/2-year old boy. The study outlines the characteristic clinical features of the syndrome, i.e. round face, upslanting palpebral fissures, microretrognathia, normal growth, slight psycho-motor retardation and congenital heart defects. The association of the der(20) inv(20) (p112q133) mat and brachymesophalangy of index ("Mohr-Wriedt" type of brachydactyly) enables the authors to suggest that chromosome 20 may be held responsible for this particular malformation.

Child, Preschool↗
PubMed1984

Hypophysectomy and growth hormone receptors in liver membranes of male rats.

The effects of hypophysectomy on GH binding were studied in liver membranes of male rats. Ten days after surgery, the specific binding of [125I]iodobovine GH and of [125I] iodohuman GH was 2- to 3-fold higher in microsomal membranes of hypophysectomized rats than in membranes of control male animals. The number of receptors rather than the affinity of the binding was affected. A nonspecific membrane effect due to hypophysectomy is unlikely since membrane markers such as 5' nucleotidase, galactosyl transferase, and insulin binding were not different in liver membranes of hypophysectomized and control rats. The somatogenic specificity and the subcellular distribution of the binding sites were not altered by hypophysectomy; the number of the GH binding sites were increased in plasma membranes as well as in Golgi fractions. Hypophysectomy in male rats creates a situation where growth failure, absence of circulating GH, and lack of plasma somatomedin activity are associated with increased concentration of liver somatogenic receptors. The latter finding could explain why livers of hypophysectomized rats are more sensitive to GH than those of normal rats.

5'-Nucleotidase↗
PubMed1983

[Pure trisomy 13q13-qter caused by aneusomic recombination of a maternal pericentric inversion].

A 17-year-old girl with a severe mental retardation and facial dysmorphism was found to be carrier of an abnormal chromosome no. 13. Her mother and several other members of her family carry a pericentric inversion of chromosome no. 13. The abnormal 13 of the proposita was shown to result of "aneusomie de recombinaison" of the inverted chromosome no. 13. Biochemical, hematological, and clinical features contribute to the establishment of the clinical map of chromosome 13.

Adolescent↗
PubMed1983

[Non-Hodgkin lymphoma associated with a relapse of Hodgkin's disease].

We report the case of a patient treated for Hodgkin's disease (stage IIIEA) by combination of chemotherapy (MOPP regimen) and total nodal irradiation. Five months after the completion of radiotherapy the patient presented with a relapse of his Hodgkin's disease and simultaneous occurrence of diffuse non Hodgkin's lymphoma in a different lymph node territory. The relationship between these two malignancies is discussed and the literature is reviewed.

Combined Modality Therapy↗
PubMed1982

Retinoblastoma, deletion 13q14, and esterase D: application of gene dosage effect to prenatal diagnosis.

Esterase D (ESD) gene dosage studies were performed on amniotic cells from a fetus at risk for del 13q14. The mother was a balanced carrier of an insertion in chromosome #20: 46,XXins(20;13)(p12;q1307q14.3). She had already given birth to a monosomic child with retinoblastoma (Rb) and to a phenotypically normal child trisomic for the same 13q14 segment. Both sibs displayed the expected proportionate gene dosage effects for ESD. A 153% value of ESD activity was found in the amniotic cells indicating unambiguously that the fetus was not monosomic for segment 13q14 and therefore not at increased risk for Rb. The mother delivered a phenotypically normal child who was confirmed to be trisomic for segment 13q14 by cytogenetic analysis and by gene dosage studies for ESD in cord blood cells and in lymphoblastoid cells.

Amnion↗
PubMed1982

[Intensifying screens: speed, definition, afterglow and abrasion resistance (author's transl)].

Main characteristics of radiological intensifying screens were measured. Speed and definition under conditions described before. The different screens-film systems were ranged after their speed factor, at 40, 70 and 120 kV with mention of definition of these systems when measured. Afterglow and abrasion resistance were measured under experimental well definite conditions. Results show that are extant rare earth screens allowing a decrease of X-ray exposure without too great afterglow or mechanical frailty.

Humans↗