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Biomedical subjects

F N Ghadially

Publications and source records attributed to F N Ghadially.

At least 55 records · Page 3Linked to original sources
PubMed1982

The morphology and atomic composition of aurosomes produced by sodium aurothiomalate in human monocytes.

Aurosomes were found in monocytes (from the peripheral blood of man) incubated with sodium aurothiomalate. In electron micrographs the aurosome presents as a single-membrane-bound lysosomal body with an electron-lucent or medium density matrix in which lie electron-dense filamentous (straight or curled), rod-like and lamellar profiles studded with particles and granules. Similar deposits did not develop in monocytes incubated with sodium thiomalate but myelinoid membranes and rod-like structures presumably derived from them were seen in the lysosomes of these cells. The aurosomes (and their characteristic electron-dense contents) produced in the monocytes in vitro were morphologically indistinguishable from those known to occur in various tissues of man and experimental animals treated with soluble goldsalts. The atomic composition of the aurosomes produced in vitro appears to be similar to that produced in vivo for in both instances Au, P and S can be demonstrated in the aurosome. It is concluded that the aurosomes produced in our experimental model is an accurate copy of that found in in vivo situations.

Electron Probe Microanalysis↗
PubMed1982

Ultrastructure and electron-probe x-ray analysis of the pigment in melanosis duodeni.

A patient with peptic ulceration developed melanosis duodeni, which was studied by light microscopy, histochemistry, electron microscopy and electron-probe x-ray analysis. Our studies show that the pigment in melanosis duodeni resides in the lysosomes of macrophages in the lamina propria. The pigment is not melanin or a melanin-like compound as has been claimed by previous workers. On the basis of electron-probe x-ray analytical findings we have concluded that the pigment is essentially FeS. On the basis of the history of this patient and cases reported in the literature we think that the Fe in this pigment is derived from haemorrhage in the gastrointestinal tract. We also found small amounts of Ca, K, Al, Mg, Si, and Ag. It would appear that Al, Mg and Si were probably derived from the biological milieu and/or other drugs and/or food and/or specimen contamination.

Cytoplasmic Granules↗
PubMed1982

The effect of uranyl acetate on human lymphoblastoid cells (RPMI 6410) and HeLa cells.

RPMI 6410 cells and HeLa cells were exposed to uranyl acetate. In RPMI 6410 cell cultures this produced single-membrane-bound presumably lysosomal bodies (called "uraniosomes") containing electron-dense crystals in the cultured cells and crystalline deposits in extracellular locations. Neither uraniosomes nor extracellular uranium deposits were found in HeLa cell cultures. All uraniosomes and extracellular uranium deposits analysed by electron-probed X-ray analysis were found to contain uranium, potassium and phosphorus. Traces of sulphur were detected in some but not all uraniosomes and extracellular uranium deposits. Traces of calcium were found in all extracellular uranium deposits and in some uraniosomes also.

Calcium↗
PubMed1982

Giant melanosomes in the eye of the Dutch rabbit.

Giant melanosomes measuring up to about 5.1 micrometers in diameter were seen in the eyes of rabbits with metallic implants. Somewhat smaller giant melanosomes up to about 2.7 micrometers in diameter were seen in the normal eye of a rabbit. Such melanosomes were not found in eyes of rabbits less than a day old. Some giant Stage IV melanosomes appear to derive from giant Stage III melanosomes with an internal structure similar to that in the normal smaller melanosomes, while others appear to be derived by fusion of smaller melanosomes. There may be yet another mechanism of production of giant melanosomes involving 'vesicular' structures but about this one cannot be certain.

Aging↗
PubMed1981

Aurosomes produced by sodium chloroaurate.

Aurosomes were produced in the rabbit synovial membrane by injecting sodium chloroaurate. They contained electron-dense, filamentous, rod-like and lamellar profiles studded with particles and granules. These aurosomes are morphologically indistinguishable from aurosomes produced by sulphur-containing soluble gold salts such as sodium aurothiomalate and aurothioglucose. It is therefore concluded that the sulphur-containing portion of these molecules is not responsible for the characteristic morphology of the aurosome as claimed by others. However, electron-probe x-ray analysis of aurosomes produced by sodium chloroaurate showed the presence of gold, phosphorus and sulphur. It is concluded that the sulphur and phosphorus must have been derived from the biological milieu, since it could not have been derived from the injected compound.

Animals↗
PubMed1981

Intramatrical lipidic debris and calcified bodies in human semilunar cartilages.

Intramatrical lipid debris and calcified bodies were found in the matrix of injured and uninjured portions of semilunar cartilages obtained from patients ranging in age from 16 to 60 years. Most of the calcified bodies were homogeneously electron-dense and they were roughly spherical or cuboidal in shape. Much rarer was the occurrence of calcified bodies containing long, slender crystals. Electron-probe X-ray analysis showed that the homogeneously electron-dense bodies contain phosphorus, calcium and magnesium.

Adolescent↗
PubMed1981

Platinosomes produced in cultured cells by platinum coordination complexes.

Cells in culture were exposed to cis-dichlorodiammineplatinum (II) and platinum-uracil blue. No platinum could be demonstrated in an intracellular location morphologically or by electron-probe x-ray analysis in the case of the former compound. However, platinum was readily demonstrated in phagosomes or phagolysosomes after exposure of cells to platinum-uracil blue.

Cell Line↗
PubMed1981

Haemosiderin deposits in the human cornea.

A siderotic cornea from a case of post-traumatic hyphaemia showed large numbers of siderosomes in the corneal fibroblasts and a few in the corneal epithelial and endothelial cells. Electron-probe x-ray analysis revealed iron, phosphorous and sulphur in the siderosomes.

Adolescent↗
PubMed1980

Genesis of concentric laminated inclusions in the nucleus.

Single-membrane-bound inclusions containing zymogen granules were found in pancreatic acinar cells. Images were seen suggesting that the concentric laminated inclusion is derived by hydration and fusion of such granules within the inclusion.

Cell Fusion↗
PubMed1980

Amianthoid fibres in a chondrosarcoma.

Amianthoid fibres up to about 1000 nm thick were found in the matrix of a chondrosarcoma. Our findings support the idea that this is a degenerative change and that these fibres are probably formed by coalescence of collagen fibrils.

Adult↗
PubMed1980

Electron-probe x-ray analysis of granules and particles found in aurosomes produced by colloidal gold.

Aurosomes produced in the rabbit synovial membrane after intraarticular injection of colloidal gold were found to contain large spherical electron-dense granules and fine electron-dense particles. Electron-probe x-ray analysis demonstrated the presence of gold in the granules and iron in the particles. Sulphur and phosphorus were not detected in these aurosomes produced by colloidal gold. This is in contrast to the aurosomes produced by the soluble gold salt sodium aurothiomalate where besides gold, sulphur and phosphorus are easily detected.

Animals↗
PubMed1980

Enzyme content of hepatocellular lysosomes in the liver of the tumour-bearing mouse.

An increase in the number of hepatocellular lysosomes was not detected in mice bearing the solid or ascites forms of Sarcoma 180 or Ehrlich's tumour. Despite this, biochemical studies showed significant increases in acid phosphatase, cathepsin-D and beta-galactosidase. This difference between morphological and biochemical observations is explained by the fact that only gross increases in lysosome population are likely to be detected in the small samples examined with the electron microscope, but by biochemical methods whereby many grams of tissue are analysed finer changes may be detected. As far as is known at present there is an increase in hepatocellular lysosomes and/or lysosomal enzymes in tumour-bearing mice, rats and man and no exception to this exists.

Acid Phosphatase↗
PubMed1980

Thickening of the nuclear fibrous lamina in injured human semilunar cartilages.

A morphometric study was carried out to assess the thickness of the fibrous lamina in the nuclei of cells adjacent to tears in semilunar cartilages and in cells from relatively normal uninjured parts of semilunar cartilages. Comparisons of mean thickness of the lamina showed a statistically significant thickening of the lamina at the site of injury. This observation supports the growing body of evidence indicating that the fibrous lamina is not a static structure of fixed dimensions for a given cell type but a dynamic structure whose thickness varies as a result of altered physiological or pathological states.

Cell Nucleus↗