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Biomedical subjects

F Leynadier

Publications and source records attributed to F Leynadier.

At least 145 records · Page 8Linked to original sources
PubMed1984

[Sensitization by repeated skin tests with candidin and PHA].

Intra-dermal skin tests (IST) were applied with candidin (1:100) in 37 subjects and repeated 10 days later. The induration diameter of the second injection (read at 48 h) was greater (p less than 10(-8]. A correlation (r = 0.76, p less than 0.001) appears between the first and second induration diameters. In 17 of these patients (randomly distributed) we measured the absolute number of mononuclear cells bearing receptors of Fc IgG (RFc) before the first and before the second injection. The number of RFc was greater (p less than 0.02) after the second injection. It may be possible that candidin testing increase T suppressor cells. In 20 other subjects, we observed an increase of induration diameter (p less than 0.05) after a second IST of PHA (2 micrograms) applied 15 days after the first injection. Repeated IST with candidin and PHA induce a very high level of booster phenomenon. This fact may be kept in mind when interpreting serial IST in immunodeficiencies.

Aged↗
PubMed1983

[Human basophil degranulation test and double diffusion in pulmonary hydatidosis].

A slide human basophil degranulation test (HBDT) and a double-diffusion test were done in 37 patients with suspected pulmonary hydatid disease. Among the 31 patients in whom a diagnosis was firmly established, 22 had a pulmonary hydatid cyst and 9 did not (controls). The HBDT was at least 35% in 17 of the 22 patients with hydatid disease; 16 of these exhibited a precipitation arc. Among the 9 controls, none had a HBDT above 30% and three had a precipitation arc. In this localization of hydatid disease, compared to the double-diffusion technique, the HBDT has similar sensitivity (0.77 versus 0.73 but higher specificity (1 versus 0.67).

Basophils↗
PubMed1983

[Test of human basophil degranulation. The interpretation and analysis of variability factors in the absence of antigen].

The human basophil degranulation test (TDBH) is performed using a concentrated cell suspension enriched for polymorphonuclear basophils. This preparation is placed in wells on a glass slide with an allergen solution of decreasing concentration. After incubation the cells are fixed in the wells and stained with toluidine blue. Preparations containing fewer metachromatic basophils than in control preparation of the same cell suspension without antigen contain degranulated basophils and in this way a degranulation index can be calculated. By considering the variability in the number of basophils between duplicate control wells from the same cell suspension, it can be shown that the test can be considered to be positive only if the number of degranulated basophils equals or exceeds 50% of the total number of basophils. This variance can be attributed to the subjectivity of the reader, and to differences in the differential cell counts from well to well. On the other hand, the presence of atopic allergy had no effect on the basophil counts.

Adolescent↗
PubMed1982

[Spasmophilia?].

Explore the source record for details and available documents.

Electromyography↗
PubMed1982

[Hypereosinophilia in cancer and cirrhosis : utility of total eosinophil count (author's transl)].

A total eosinophil count (TEC) and a white blood cell count (WBCC) were performed simultaneously in 213 patients. Discrepancies were found between TEC and WBCC. WBCC did not allow detection of hypereosinophilia (TEC exceeding 300 eosinophils/microliter) in 45 patients. Patients were classified in three groups : controls (n = 27), benign disease (n = 65), and apparently cured cancer (n = 36). There was no significant difference between total eosinophil counts for these three groups (Wilcoxon non-parametric test). The incidence of hypereosinophilia (TEC exceeding 300) was similar in the three groups (11%, 14% and 6%). Average TEC levels were determined in 20 patients with decompensated hepatic cirrhosis, in 31 patients with active cancer, and in 14 patients with infection. These levels were significantly different from those found in each of the three previous groups. (Wilcoxon test). The incidence of hypereosinophilia (greater than 300/microliter) was significantly higher among patients with cirrhosis (50%) or active cancer (58%). The possible mechanisms of this hypereosinophilia and it's eventual prognostic utility are considered.

Adolescent↗
PubMed1981

Automated measurement of human basophil degranulation.

Basophils disappear after challenge with specific antigen. A human basophil degranulation test has been carried out on a slide using an enriched cell suspension. On each slide, the same volume of cell suspension was deposited in wells with antigen diluted in buffer or with buffer only (control). The basophil count was made on an equal number of randomly distributed microscopic fields either by eye (optical) or by image analyser (automatic). In 33 subjects, the correlation coefficient between the numbers of non-degranulated basophils counted by eye and by image-analyser on control wells was found to be r-0.91, p less than 10-8. In 12 patients (6 with hydatidosis and 6 with schistosomiasis), the percentage of degranulation with three antigen dilutions was measured. The correlation between the results obtained by eye and by image-analyser reached 0.76 (p less than 10-7). The authors now use the automated measurement of human basophil degranulation routinely.

Autoanalysis↗
PubMed1980

[Minor toxic effects of local anaesthetics. 7 cases (author's transl)].

In a study of 7 cases of adverse reactions to local anaesthetics the early symptoms of toxicity (malaise, anxiety, feeling of impending death, peripheral paresthesias and tachycardia) are reviewed. Minor toxic reactions produced by these drugs are common, whereas immediate allergic reactions are rare and even, for some authors, non-existent with lignocaine and derivatives. Biological investigations aimed at demonstrating the "allergic" nature of these reactions are valueless and costly.

Adult↗