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Biomedical subjects

F J Kaup

Publications and source records attributed to F J Kaup.

At least 73 records · Page 4Linked to original sources
PubMed1992

[Scanning electron microscopic findings of the navicular bone and deep flexor tendon in podotrochlosis of horses].

In 25 adult horses the podotrochlea of 49 forelimbs was examined by means of light and electron microscopy in order to correlate clinical and morphological findings. According to the clinical diagnosis the animals were divided into three groups: lameness due to syndrome of podotrochlosis (group 1) or due to tendopathy (group 2) and horses without lameness (group 3). The most striking pathological findings of the navicular bone and the opposite surface of the deep flexor tendon were found in horses with podotrochlosis, consisting of loss of cartilage and tendon matrix with denudation of collagen fibrils, superficial degeneration and necrosis, focal occurrence of fissures and far-reaching defects, sometimes accompanied by adhesions between tendon and navicular bone surface. In radiologically only slightly altered tendons and navicular bones light and scanning electron microscopy often revealed moderate to severe pathological changes, which sometimes could also be seen in clinically healthy horses. The distribution of the lesions indicate that in course of podotrochlosis the initial alterations caused by repeated microtraumata start at the surface of the deep flexor tendon and subsequently spread to the opposite facies flexoria of the navicular bone. An involvement of the cartilage and the synovial layer of the hoof joint in cases with podotrochlosis could only be rarely observed and is to regard as a secondary manifestation.

Animals↗
PubMed1991

Identification of a superoxide-generating NADPH oxidase system in human fibroblasts.

Human fibroblasts have the capacity to release superoxide radicals upon stimulation of an electron transport system similar to the NADPH oxidase of leukocytes. Two components of the NADPH oxidase system, (1) a flavoprotein of 45 kDa which binds diphenylene iodonium (a compound described as a specific inhibitor of the leukocyte NADPH oxidase), and (2) a low-potential cytochrome b, are present in fibroblast membranes. Fibroblasts exhibit these compounds at lower concentrations than do polymorphonuclear leukocytes or B-lymphocytes. The superoxide-generating system is rather uniformly associated with the outer cell membrane, as shown by light and electron microscopy. Superoxide release upon stimulation with various agents was prevented by the addition of micromolar concentrations of diphenylene iodonium, making an NADPH oxidase a likely source.

Calcimycin↗
PubMed1991

Morphological investigations on the pathology of Dictyocaulus viviparus infections in cattle.

Morphological investigations of lungs obtained from cattle that had been experimentally infected with Dictyocaulus viviparus were carried out using a new fixation technique that excludes post-mortem changes in the samples. Light and electron microscopical investigation showed a substantial loss of ciliated epithelial cells in the bronchi, severely affecting the mucociliary clearance. Bronchiolitis, peribronchiolitis and cell-rich mucus caused bronchial atelectasis and consolidation of the related alveoli. Interlobular septa completely separated diseased from normal tissue. Clara cells contained abundant intraplasmatic glycogen. Alveolary walls were thickened and showed cellular infiltration, loss of functional epithelial cells and hyperplasia of alveolar type II cells. Increased connective tissue indicated an irreversible chronic decline in lung performance. Lung eosinophilia and the cytotoxic abilities of eosinophils are discussed as a cause of the changes in lung tissue after infection with D. viviparus.

Animals↗
PubMed1991

Ioxaglate-induced light and electron microscopic alterations in the renal proximal tubular epithelium of rats.

Vacuolization of the proximal tubular epithelial cells was produced in rats by the intravenous administration of the radiographic contrast medium ioxaglate at high multiples of the human diagnostic dose. Samples of the renal cortex and outer zone of the medulla were examined by light and electron microscopy. We observed enlargement, confluence, and migration of vacuoles containing pleomorphic dense material and distinct inclusion bodies. With time, vacuolization disappeared, though single vacuoles partly engaged in extruding their contents into the tubular lumen were still visible. We concluded that radiographic contrast medium at high dose levels can produce a reversible disturbance in the transport vesicular system of the proximal tubular epithelial cells without affecting the specific cell organelles.

Animals↗
PubMed1991

[Which evidence permits a liver cell smear in cattle with metabolic disorders?].

The technique of liver puncture as described by Holtenius (1961) was assessed with regard to its practicability and safety in a preliminary study of 26 cattle and a follow-up study of 108 German black pied cattle. The sample material was smeared, stained and examined by light microscope. All animals with changes of grade 5 had to be slaughtered. Liver puncture is a simple, quick and safe technique which supplies useful information about the various stages of hepatic fatty degeneration in individual cattle.

Animals↗
PubMed1990

Characteristic of spontaneous intraperitoneal cysts in golden hamsters and European hamsters.

Epidemiological study of 340 Golden hamsters and 200 European hamsters bred as laboratory animals and necropsied between 1985 and 1989 in our facility (affiliation 2) revealed high incidence of spontaneous cysts. The incidence was in general higher in European (26 to 62%) than Golden hamsters (0 to 15%). The cysts, of different sizes, were localized first of all in the liver, and secondly (in European hamsters only), in the caecum wall. Light microscopic and electron microscopic examinations showed that the cysts are lined by flattened to cuboidal epithelial cells which, on their surface, have microvilli of different lengths and underneath a continuous basement membrane. These examinations lead further to the interpretation of the liver cysts as cystic distensions of the bile ducts. The cysts in the caecum wall could be interpreted as dilatations of the lymphatic vessels. As to the etiology, which remains unclear, several hypotheses are discussed.

Age Factors↗
PubMed1990

Ultrastructural findings in horses with chronic obstructive pulmonary disease (COPD). I: Alterations of the larger conducting airways.

Extensive light and electron microscope studies of the conducting airways were carried out in 28 horses with varying degrees of clinically manifested chronic obstructive pulmonary disease (COPD) and in 8 horses with normal lungs. The principal ultrastructural changes were found in the ciliated cells. There was focal loss of ciliated cells, which were replaced by undifferentiated cells in a largely hyperplastic epithelium, and some horses, independent of the degree of severity of the disease, showed various types of ciliary malformation. The finding of dilated intercellular clefts and accumulations of mast cells was interpreted as morphological evidence of non-specific mucosal hyperreactivity. Interstitial cells with intracytoplasmal crystal inclusions, the cause of which is not clear, were seen in many horses. Comparison between the clinical diagnosis and the morphological findings showed partial correlation. The ciliary loss, the appearance of peribronchial inflammatory processes and the occurrence of intracytoplasmic crystalline inclusions showed a positive correlation. In 12 of 28 cases, the severity of clinical diagnosis agreed with the degree of the morphological findings. The changes in the conducting airways were interpreted pathogenetically as reactive processes to changes in the small airways in the course of equine COPD.

Animals↗
PubMed1990

Ultrastructural findings in horses with chronic obstructive pulmonary disease (COPD). II: Pathomorphological changes of the terminal airways and the alveolar region.

Extensive light and electron microscope studies (transmission and scanning electron microscopy) of the bronchioles and alveolar region, in 28 horses suffering chronic obstructive pulmonary disease (COPD) and eight control horses, revealed good correlation between clinical severity and morphological changes. In the bronchiolar epithelium the non-ciliated bronchiolar epithelial (Clara) cells, in particular, showed ultrastructural alterations and, even in the mild stages of disease, these presented degenerative changes and lack of differentiation. Together with loss of granulation in the Clara cells and metaplasia of the goblet cells, cells were seen with unusual intracytoplasmic lamellar inclusion, the number of which increased sharply with clinical severity. The focal changes in the alveolar region were necrosis of type I epithelial cells, alveolar fibrosis of varying degrees with type II epithelial transformation and emphysema or hyperinflation, with an increase in Kohn's pores. Some horse also showed morphological signs of interference with the surfactant system, in the form of marked cysts with lamellar structure. The alveolar changes were mostly in the peribronchiolar region and were, therefore, interpreted as reactive processes. No conclusions as to the aetiology of equine COPD can be derived from these morphological investigations.

Animals↗
PubMed1990

[Corynebacterium suis infections in swine. 2. Morphological findings in the urinary tract with special reference to the bladder].

Morphological investigations of the urethra, urinary bladder (2 localizations), ureter, renal pelvis and kidneys were performed in sows with Corynebacterium suis infection. The first stages of the disease were characterized by changes of the transitional epithelium in the bladder where epithelial vacuolisation, goblet cell differentiation, leucocytic infiltrations, and haemorrhages occurred. In chronically affected animals different stages of a mucopurulent, partly erosive to ulcerative cystitis were observed. Furthermore, chronic cystitis was characterized by polypoid mucosal proliferation and bacterial accumulations. Ureter and urethra showed signs of a mild purulent inflammation. In the renal pelvis there was a severe fibrinopurulent and necrotizing pyelitis which was accompanied by bacterial invasions. The parts of the renal pelvis without desquamation of the transitional epithelium undergo mucoid degenerations. The final stage of the disease is characterized by severe fibrosing chronic interstitial nephritis leading to death of the animals due to uraemia.

Animals↗
PubMed1989

[Electron microscopic structure of mesangioproliferative glomerulonephritis (MesPGN) in animals].

In a murine lupus model, the kidneys of 42 MRL mice of different age groups (substrains MRL/Mp-lpr/lp and -+/+) were studied by light and transmission electron microscopy. The results demonstrate the ultrastructural feature of the mesangial-proliferative glomerulonephritis which is one of the observed forms of glomerulonephritis. The most important alterations are a severe proliferation of mesangial cells (with an increase in rough endoplasmic reticulum and mitochondria) and electron dense deposits in different sites of the glomerular basement membrane (subepithelial, subendothelial, intramembranous). These deposits are proposed to be immune complexes. Osmiophilic intracytoplasmic inclusions of the mesangial cells are indications of an altered renin production. Alterations of the glomerular epithelial cells are characterized by fusions of the epithelial pedicles, an increase of microvilli and intracytoplasmic concentrations of electron dense material which are also proposed to be immune complex deposits. The morphological feature of the mesangial-proliferative glomerulonephritis is completed by an activation and focal edema of endothelial cells. The described alterations are discussed and compared with findings in other species.

Animals↗
PubMed1986

The role of cartilage canals in the pathogenesis of experimentally induced polyarthritis.

Porcine articular cartilage from cases of experimentally induced Erysipelas polyarthritis, a comparative model of rheumatoid arthritis (RA) in man, was examined with different histological and immunohistochemical techniques. The preexisting canals in articular cartilage played a crucial role during the flooding and deposition of arthritogenic microorganisms deep into the cartilage matrix. Subsequently this vascularized tissue mediated the same inflammatory reactions in hyaline cartilage of young animals as seen in other connective tissues. However, these stereotypical responses to injury were modulated by the unique composition and structure of articular cartilage.

Animals↗