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Biomedical subjects

F Inoue

Publications and source records attributed to F Inoue.

At least 109 records · Page 6Linked to original sources
PubMed1987

Demonstration of non-neural tryptophan 5-mono-oxygenase in mouse intestinal mucosa.

Tryptophan 5-mono-oxygenase was demonstrated and its activity was measured in mucosal extracts of the mouse digestive tract by means of highly sensitive h.p.l.c. detection. The intestinal enzyme was activated by anaerobic incubation with dithiothreitol, as are the enzymes from mouse mastocytoma cells and bovine pineal gland. The dithiothreitol-enhanced activity was highest at the proximal portion of colon followed by that at the duodenum, where the unenhanced activity/enhanced activity ratio was highest. The enzymic and immunochemical properties of the intestinal tryptophan 5-mono-oxygenase were similar to those of the mastocytoma enzyme. In contrast, the intestinal enzyme was immunochemically different from brain tryptophan 5-mono-oxygenase. The possibility that connective tissue and/or mucosal mast cells are responsible for some of the enzyme activity of the duodenal mucosa was ruled out by the demonstration of the activity in extracts from a mast-cell-deficient mutant mouse (W/Wv). The enzyme in the duodenum was found to reside between the upper villus region and the bottom of the crypt, suggesting that it is mainly of enterochromaffin cell and not of submucosal nerve plexus origin.

Alkaline Phosphatase↗
PubMed1987

Activation of deoxycholic acid by the lipid peroxy radical and its covalent binding to nucleic acids.

Deoxycholic acid, a colon tumor promoter, was found to bind covalently to DNA and RNA in the presence of methyl linoleate hydroperoxide and ferrous ion. This binding was shown to occur specifically with guanine residues and its covalent nature was demonstrated by analysis of hydrolysates of nucleic acid adducts. These findings are discussed in connection with the increased risk of colon cancer associated with a high fat and meat diet.

DNA↗
PubMed1987

The occurrence of N-methyl-D-aspartic acid in muscle extracts of the blood shell, Scapharca broughtonii.

A novel ninhydrin-positive compound, N-methyl-D-aspartic acid, was identified in the muscle extracts of the blood shell, Scapharca broughtonii. This compound is already known to have potent neuroexcitatory activity, inducing hypermotility and strong releasing action of serum luteinizing hormone in mammals. This may be, however, the first finding of N-methyl-D-aspartic acid in natural products.

Animals↗
PubMed1987

Natural killer activity of peripheral blood lymphocytes and its relation to histopathological factors of lung cancer.

This investigation was intended to determine whether the natural killer (NK) activity of peripheral blood lymphocytes (PBL) correlated with the histopathological factor, which is thought to be a result of a balance between tumor aggression and host resistance. The NK activity of PBL from 60 patients with lung cancer was measured by the lysis of 51Cr-labelled K562 target cells. The activity was significantly decreased with advancing stages of the disease, and inversely correlated with increased immunosuppressive substance levels of the serum. Histopathological factors, such as low grade pleural invasion of the tumor and abundant lymphoid cell infiltration around the tumor, were significantly associated with the high NK activity of PBL. These results show that a decrease in NK activity may play a role in identifying those individuals with a greater risk of cancer development.

Cytotoxicity Tests, Immunologic↗
PubMed1987

Endogenous catabolism is the major source of toxic metabolites in isovaleric acidemia.

A patient with isovaleryl-coenzyme A dehydrogenase deficiency was given a synthetic oral feed containing L-(2H3-methyl)-leucine of high isotopic purity as the only dietary precursor to the defective enzyme. Metabolites derived from this source were readily distinguished from their unlabeled endogenous counterparts by mass spectrometry. During 6 consecutive days of labeled leucine ingestion, the average daily excretion of labeled metabolites was only about 10% of the total derived from leucine. It is suggested that therapy should be directed toward the control of endogenous protein turnover rather than the restriction of dietary protein intake.

Amino Acid Metabolism, Inborn Errors↗
PubMed1987

Purification and some properties of two kinds of keratin-hydrolyzing enzymes of cow snout epithelium.

Two kinds of keratin-hydrolyzing enzymes (KHEs) from cow snout epithelium were highly purified by affinity chromatography using soybean trypsin inhibitor-bound Sepharose. On gel filtration chromatography, the KHEs were eluted at a volume corresponding to a relative molecular mass (Mr) of 21,000. They were separated from each other by ion exchange chromatography. One of the enzymes had the same characteristics as urea extracted alkaline proteinase, of which optimal pH was at 8.5 to 9.0. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme showed a single band with Mr of 21,500 in the presence or absence of a reducing agent. The other enzyme was a neutral proteinase, with an optimal pH of 7.5. Both enzymes were inhibited by phenylmethylsulfonyl fluoride and soybean trypsin inhibitor. Among the fluorogenic peptides that were hydrolyzed most effectively by the alkaline proteinase were peptidyl MCAs (4-methyl-coumaryl-7-amides) with extended sequences, Boc-Leu-Ser-Thr-Arg-MCA, and then Boc-Val-Pro-ARg-MCA. The neutral proteinase hydrolyzed the latter most effectively. They hydrolyzed preferentially high Mr keratins of cow snout and of newborn mouse epidermis, and showed a limited proteolysis toward 68,000 polypeptide, giving rise to distinct products. The high substrate specificity and extended subsites of the KHEs suggest their role on the metabolism of the high Mr keratins.

Animals↗
PubMed1987

[Nursing attitude].

Explore the source record for details and available documents.

Attitude of Health Personnel↗
PubMed1986

Serotonin in the lung. Demonstration of a close correlation to blood platelet.

The dependency of a high level of serotonin (5-hydroxytryptamine, 5HT) in the lung on blood 5HT was examined. The whole lung excised from NOD/Shi mice (male, 8-12 week old) immediately after being sacrificed by cervical dislocation contained 2.86 +/- 1.21 micrograms/g of 5HT per wet tissue. This amount was much higher than that explained by the included blood which was estimated by the hemoglobin concentration in the lung extract. In order to decrease 5HT level in the blood, platelet was depleted by an exposure to gamma-ray. On the 10th day after the irradiation the amount of 5HT in the lung and blood decreased to about 3.1% and 1.5% of the respective normal values. The progressive decrease in 5HT in the lung and blood of the irradiated mice was prevented by the transplantation of normal bone marrow cells (10(7) cells/mouse). 5HT in the intestine did not change significantly. The correlation between 5HT in the excised lung and the number of platelets in the blood was statistically significant (correlation coefficient r = 0.61). The irradiated, platelet-deficient mice were incapable of accumulating serotonin in the lung, while normal mice increased the lung serotonin more than 3-fold, when high doses of 5HT were administered. The results indicated that the high level of 5HT in the dissected lung was closely related to platelet in the blood. A question remained where the majority of the measured 5HT was located in the lung tissue.

Animals↗
PubMed1986

[An autopsy case of adult T-cell leukemia with acute hepatic failure].

An autopsy case of ATL with acute hepatic failure is reported. A 39-year-old man born in Miyazaki Prefecture was admitted because of jaundice and general malaise of about 10 days' duration. Palpation of the abdomen revealed moderate hepatomegaly: the patient had clear consciousness. The white cell count was 62,500/mm3, with 34% abnormal lymphocytes having lobulated or convoluted nuclei. Marked liver dysfunction (TB 30.5 mg/dl; GOT 2,740 U; GPT 1,031 U; LDH 3,833 U) was noted. He developed hepatic coma and died of acute hepatic failure on the third hospital day. At autopsy, the enlarged liver weighed 2,000 g and was diffusely infiltrated by ATL cells.

Acute Disease↗
PubMed1985

Purification of opioid-binding materials from rat brain.

Opiate receptors were solubilized from rat brain with digitonin and treated with an affinity resin, AH-Sepharose coupled with [D-Ala2, Leu5]enkephalin. The eluted materials from the resin with the opioid peptide had opioid-binding activity. Based on the protein content of the purified materials, 450-fold purification over the solubilized receptors was achieved. Analyses by gel electrophoresis revealed that the purified materials were rich in one polypeptide with a molecular weight of 62,000.

Animals↗
PubMed1985

Rimmed vacuoles in biopsied muscle of nemaline myopathy.

A case of nemaline myopathy with rimmed vacuoles was presented. Muscle biopsy revealed type I fiber predominance with type grouping, thus suggesting that neurogenic factors played a pathologic role in the degenerative change.

Biopsy↗
PubMed1985

Distribution of 5-hydroxytryptamine (5HT) in tissue of a mutant mouse deficient in mast cell (W/Wv). Demonstration of the contribution of mast cells to the 5HT content in various organs.

The content of 5-hydroxytryptamine (5HT) in various tissues of mutant mouse (W/Wv) deficient in mast cells and of control mouse (+/+) was determined by high performance liquid chromatography. The depletion of mast cells in the mutant mouse (W/Wv) was expected to cause a decrease in the 5HT content. In the control mice, 5HT was most densely accumulated in the lung (9.66 +/- 5.23 micrograms/g). Large intestine (6.40 +/- 2.61 micrograms/g) and stomach (6.10 +/- 2.14 micrograms/g) followed the lung in the rating of the 5HT content. The 5HT content of W/Wv mice was only 23.4% and 4.1% that of the control in the stomach (p less than 0.01) and the skin (p less than 0.01), respectively. The results were consistent with the expectation. In other organs (small intestine, caecum, large intestine, brain, lung, blood and salivary gland), the difference between the W/Wv and normal mice was not statistically significant. The difference in the 5HT content of the stomach between the two genotypes was 4.67 micrograms/g and was much larger than the 5HT content (0.49 micrograms/g) of normal mouse skin. With regard to the relatively small number of mast cells present in the stomach, the great difference in the 5HT content in the stomach between the two genotypes cannot be explained by the loss of mast cells. Hence, besides mast cells other cells may contribute to the high 5HT content of the stomach.

Animals↗