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Biomedical subjects

F H Bach

Publications and source records attributed to F H Bach.

At least 541 records · Page 30Linked to original sources
PubMed1969

Cell kinetic studies in mixed leukocyte cultures: an in vitro model of homograft reactivity.

The recognition phase of homograft immunity can be studied in vitro in the mixed leukocyte culture reaction. In this reaction, at the end of seven days, up to 30 per cent of the lymphocytes in culture "respond" to a single allogeneic cell stimulus. This paper presents evidence that the lymphocytes responding in culture divide with the generation time of 18-21 hours, with some asynchrony, and that a large percentage of the cells found at the end of the culture period may be the products by division of a small number of cells initially responding. Two estimates are made of the frequency of the initially responding unit (the cell(s) responsible for initiating the response). These are probably minimum estimates and are in the range of 1 in 200 to 1 in 2000 cells which can initially respond. This very high frequency of responding units as compared with the responding units in immediate type hypersensitivity (antibody production) is discussed.

Autoradiography↗
PubMed1968

Phenotypic expressions of the major histocompatibility locus in man (HL-A): leukocyte antigens and mixed leukocyte culture reactivity.

The evidence is reviewed that a single genetic system, the major histocompatibility locus in man, HL-A, determines most of the antigens measured by presently available leukocyte isoantisera, and also controls reactivity in one-way mixed leucocyte culture tests. Studies in 12 families are presented to support this conclusion. Some interesting exceptions to the general typing-MLC tests correlation are presented and discussed.

Antigens↗
PubMed1967

Hu-1: Major histocompatibility locus in man.

A single locus with 15 or more alleles controls reactivity in mixed leukocyte culture tests. Genes at this locus also control most of the specificities measured by cytotoxic antiserums to leukocytes. Both of these tests can be used to predict survival of skin grafts. It is proposed that this is the major histocompatibility locus in man.

Antigens↗
PubMed1966

One-way stimulation in mixed leukocyte cultures.

We have developed an improved method for the mixed leukocyte culture test. Control values, as determined by rates of incorporation of thymidine, are very low, allowing evaluation of low levels of stimulation in homologous cell mixtures. One-way stimulation is assayed by treating the cells of one individual with mitomycin C; treated cells cannot respond (incorporate thymidine) but can still stimulate homologous untreated cells to do so.

Culture Techniques↗