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Biomedical subjects

F Gottrup

Publications and source records attributed to F Gottrup.

At least 109 records · Page 6Linked to original sources
PubMed1983

Activation of chemotactic factors in serum and wound fluid by dextranomer.

Dextranomer (Debrisan), a frequently used wound-cleansing agent, was shown to cause activation of chemotactic factors in human serum and wound fluid. These factors attracted both polymorphonuclear leukocytes (PMNs) and mononuclear leukocytes (MNs) in an agarose migration assay. When Debrisan-activated sera were used as the chemotactic stimulus, migration scores for PMNs and MNs were respectively 50% and 70% of the values obtained with zymosan-activated sera. Differential chelation studies demonstrated that Debrisan causes activation of the alternate complement pathway, and the factors produced cause receptor-mediated leukotaxis of both PMNs and MNs. Formation of chemotactic factors at the wound surface is a mechanism whereby Debrisan could conceivably augment wound healing and the inflammatory response to infection.

Chemotactic Factors↗
PubMed1983

Healing of incisional wounds in stomach and duodenum: influence of long-term healing on mechanical strength and collagen distribution.

Mechanical properties, rupture pattern (morphologic characteristics) and distribution of collagen concentration in incisional wounds of rat stomach and duodenum after 80 days of healing were compared with previous observations on 40-day wounds and with intact tissue. Increased energy absorption (breaking energy) was found in wound tissue of stomach and duodenum after 80 days. No other differences were found between 40 and 80 days of healing. The weakest point of the healing wound was 4-5 mm lateral to the incision line, in the margin of the biochemically active zone. The results indicate that the functionally important part of the healing process in stomach and duodenum occurs early, but some activity persists for at least 80 days postoperatively. The main activity in late wound healing probably is remodelling of scar tissue, completing restoration of tissue strength. Suture materials and technique are unimportant for late healing phases in stomach and duodenum. It is emphasized that a detailed biomechanical account of wound properties is necessary to describe the development of a wound's mechanical strength.

Animals↗
PubMed1982

Effect of dopamine on pentagastrin-stimulated gastric acid secretion and mucosal blood flow in dogs with gastric fistula.

The purpose of this study was to elucidate the effect of intravenously administered dopamine on dopamine receptors and adrenergic receptors in terms of its effect on gastric acid secretion, the kinetic mechanism, blood flow, and antral motility. Dopamine was used alone and in conjunction with selective blockade of alpha-, beta-, and dopaminergic receptors. A significant inhibition of gastric acid secretion was found with the highest dose of dopamine used (40 micrograms/kg/min). The kinetic study showed characteristics of a non-competitive type. The anti-secretory effect dopamine was significantly blocked by non-selective beta-blockade or by selective beta-blockade but not by alpha- or dopaminergic receptor blockade. This suggests that the inhibitory effect of dopamine on gastric secretion is mediated by beta-receptors. There was no significant effect on gastric mucosal blood flow, but the ratio between blood flow and acid secretion was significantly elevated during dopamine infusion, indicating that the acid inhibition was not secondary to changes in blood flow. It is concluded that the dopamine inhibition of acid secretion is mediated by beta 1-receptors, unlike the effect on antral gastric motility, which is mediated by dopamine receptors.

Adrenergic alpha-Antagonists↗
PubMed1981

Healing of incisional wounds in stomach and duodenum. Collagen distribution and relation to mechanical strength.

The present study was performed to determine the changes in and distribution of collagen concentration around a healing incision in rat stomach and duodenum. These concentrations were related to the mechanical properties presented previously. Wounds were made in the nonglandular (rumen) and the glandular oxyntic parts (corpus) of the stomach and in duodenum. Specimens were cut parallel to the incision line and hydroxyproline contents and dry defatted weight were measured. Wounds were investigated 50 to 40 days after operation. Of the intact tissues the nonglandular part of the stomach had twice the collagen concentration of the glandular oxyntic part and duodenum, which did not differ from each other. The healing wounds in the glandular oxyntic part of the stomach and duodenum showed the most rapid increase in collagen concentration in the incision line and 40 days postoperatively both had collagen concentrations significantly greater than those of intact tissues. Wounds in the nonglandular part of the stomach only reached the level of intact tissue after 40 days. The dimensions of the biochemically active zones around incisions in stomach and duodenum remain essentially unchanged for 40 days after operation. A relation between the development of collagen concentration and mechanical strength was shown. These findings indicate that would healing in stomach and duodenum is rapid, that collagen is the primary factor in the mechanical properties and that the highest activity is limited to a zone close to the incision line. The width of the biochemical zone remains constant.

Animals↗
PubMed1981

Healing of incisional wounds in stomach and duodenum. Collagen synthesis.

Collagen synthesis, collagen concentration and collagen content were studied 5, 7, 10, 20 and 40 days postoperatively to elucidate the turnover of collagen in healing incisional wounds in rat stomach and duodenum. Collagen synthesis was elevated relative to total protein synthesis in wound tissues compared to intact tissues and increased throughout the period with peak values at day 5. Also, collagen concentration increased while collagen content remained constant implying equal rates of collagen synthesis and degradation. This shows that the prime tissue response to wounding is changes in collagen metabolism, which correlates with mechanical strength gain.

Animals↗
PubMed1981

Effect of a beta2-sympathomimetic on urecholine-stimulated gastric acid secretion in dogs.

The effect of a selective beta2-adrenoceptor agonist on urecholine-stimulated gastric acid secretion was studied in conscious gastric fistula dogs. A dose-dependent inhibition was found, most pronounced for the highest doses of urecholine. The inhibition was primarily on volume, whereas the acidity showed a decreasing tendency only. The pulse rate was increased by the beta2-agonist and by urecholine in high doses. Propranolol prevented both the inhibition of acid secretion and the increase in pulse rate. Practolol had no effect on the inhibition of acid secretion and the increase in pulse rate. Practolol had no effect on the inhibition of acid secretion but prevented the increase in pulse rate. Dose-response experiments with five doses of urecholine and two doses of the beta2-agonist showed a decrease in maximal response, and transformation of the curve suggested an unchanged D50. It is concluded that the beta2-agonist inhibits urecholine-stimulated acid secretion in the dog to the same degree as pentagastrin-induced acid secretion, and much stronger than found for histamine-induced acid secretion in earlier experiments. The inhibition seems to follow a noncompetitive kinetic, and beta2-receptors are probable involved.

Adrenergic beta-Agonists↗
PubMed1981

Dopamine receptors do not influence beta2-sympathomimetic-mediated inhibition of gastric acid secretion in the dog.

The effect of dopamine blockade by haloperidol and metoclopramide on beta2-adrenergic-induced changes of acid secretion and pulse rate was studied in conscious gastric fistula dogs. Neither haloperidol (0.15 mg/kg and 0.60 mg/kg) nor metoclopramide (0.25 mg/kg and 1.0 mg/kg), given intravenously, had a significant influence on beta2-adrenergic-induced inhibition of gastric acid secretion or tachycardia. Dopamine blockade had no influence on pentagastrin-stimulated gastric acid secretion, but it increased pulse rate. It is concluded that dopamine receptors are not involved in the inhibiton of acid output or tachycardia induced by beta2-sympathomimetics, and dopamine is probably not a neurotransmitter for these effects.

Adrenergic beta-2 Receptor Agonists↗
PubMed1981

Effect of isoprenaline on pentagastrin-stimulated gastric acid secretion in dogs with gastric fistula.

The purpose of this study was to elucidate the effect of a beta 1-adrenoceptor agonist on gastric acid secretion in conscious dogs with gastric fistula. Isoprenaline, a beta 1- and beta 2-agonist was used alone and in conjunction with selective blockade of beta 2- and beta 1-receptors. Isoprenaline dose-dependently inhibited the secretory volume and the acidity. The antisecretory effect of isoprenaline was significantly blocked by the beta 1-adrenoceptor blocker practolol and by the beta 1 + beta 2-adrenoceptor blocker propranolol but not by H 35/25, a beta 2-adrenoceptor blocker. This indicates that isoprenaline acts on the acid secretion exclusively through beta 1-receptors. Dose-response experiments with five logarithmically increased doses of pentagastrin and one dose of isoprenaline showed unchanged calculated maximum response and an increase in half-maximum acid response. It is concluded that the inhibitory effect of isoprenaline on gastric acid secretion is of competitive or uncompetitive type.

Adrenergic beta-Antagonists↗
PubMed1981

Effect of a beta 2-sympathomimetic on gastrin release, acid secretion, and blood glucose during basal conditions and in response to insulin, 2-deoxy-D-glucose, and feeding in the dog.

The effect of a selective beta 2-adrenoceptor agonist on basal volume and on insulin-, 2-deoxy-D-glucose (2-DG)-, and food-induced gastrin release was studied in conscious gastric fistula dogs. Acid output and blood glucose changes were also studied, except in the food experiments. Basal acid secretion and serum gastrin were unchanged after beta 2-sympathetic infusion, whereas a slight increase in blood and glucose was found. The beta 2-agonist almost prevented acid output and gastrin release after insulin hypoglycaemia. However, the hypoglycaemia was also inhibited. Gastric acid secretion stimulated by 2-DG was inhibited, as was probably the gastrin release. 2-DG increased the blood glucose level, and no significant differences were found after beta 2 infusion. After feeding, gastrin release was initially decreased for one of five doses of the beta 2-agonist, and higher doses of the beta 2-agonist prevented the subsequent fall in serum gastrin after the initial peak value. This pattern was also found for the histamine H2-blocker cimetidine in a dose that blocks acid output. The beta 2-agonist and 2-DG increased pulse rate. It is concluded that beta 2-sympathetic stimulation inhibits acid output and gastrin release after insulin and 2-DG stimulation, but one should be cautions in drawing conclusions from the insulin experiments. The effect on gastrin release is small compared with the effect on the acid secretion, and it is unlikely that the inhibition of acid secretion acts through a change in gastrin release.

Animal Feed↗
PubMed1981

Healing of incisional wounds in stomach and duodenum. The influence of aging.

An evaluation was made of the effects of increasing age on the mechanical properties and the collagen content of healing wounds in stomach and duodenum of rats. Wounds were made in the non-glandular (rumen) and the glandular oxyntic part (corpus)of the stomach and in the duodenum of rats that were 26 months old. The wounds were tested 7 and 20 days after the operation. The results of these tests were compared with those made on a group of young rats (3-4 months old) that were subjected similar wounds. Aging increased the stiffness and decreased the extensibility of tissue taken from intact stomach (rumen and corpus) and from wounded rumen. No differences in the stiffness and extensibility of the tissue from the duodenum wounds could be demonstrated. Except for a higher breaking strength in samples of rumen from old animals after 20 days of healing, no differences in mechanical strength measurements (breaking strength and breaking energy) from intact and wounded tissue could be demonstrated in the old and young animals. The collagen content of intact as well as wounded stomach tissues appeared related to age. No differences in the collagen content in the tissue from the duodenal wounds wee found. The dimensions of the biochemically active zone around the incision remained essentially unchanged in the duodenum and stomach and it was of the same width as that found for young animals. This indicates that aging has no adverse influence on wound healing in the stomach and the duodenum of rats.

Aging↗
PubMed1981

Metronidazole and appendicitis. Can a preoperative prophylaxis be changed to a peroperative treatment in high risk patients?

Sixty-four consecutive patients appendicectomized for perforated appendicitis were studied retrospectively to assess the effect of peroperative metronidazolee on postoperative infections. All patients were given antibiotics against aerobic organism. The infection rate in patients receiving metronidazol peroperative was 3%. It is concluded that the effect of peroperative administrated metronidazole in prevention of postoperative infections after perforated appendicitis is as good as previously demonstrated for preoperative administrated. Consequently metronidazole treatment can be restricted to peroperative proven high risk patients with perforated and perhaps gangrenous appendicitis.

Adolescent↗
PubMed1980

Healing of incisional wounds in stomach and duodenum. A biomechanical study.

The healing pattern of incisional wounds in the rat stomach and duodenum was determined. A model allowing the biomechanical determinations of complete load-deformation curves is described. Wounds were made in the nonglandular (rumen) and glandular oxyntic (corpus) parts of the stomach and in duodenum. The wounds were tested 5 to 40 days after operation. Of the intact tissues the nonglandular part of the stomach was found to be more extensible and required more energy to be ruptured than the glandular part of the stomach and duodenum. The healing wounds in the glandular part of the stomach and duodenum showed the most rapid increase in mechanical strength, and after 40 days both required more energy to be ruptured than intact tissue. Wounds in the nonglandular part of the stomach reached only 75% of intact strength value after 40 days. The process of wound healing resulted in an increase in tissue stiffness. These findings indicate that wound healing in stomach and duodenum is more rapid than that in most other tissues and that the load-strain data give a detailed picture of the healing process. The energy required to rupture a wound represents the most informative assessment of wound healing.

Animals↗
PubMed1980

Effect of two types of beta-adrenergic blockade on gastric acid secretion during basal conditions and during histamine stimulation on non-vagotomized and in vagotomized gastric fistula dogs.

The effect of beta-adrenoreceptor blockade by propranolol and practolol on basal secretion and on submaximally histamine-stimulated gastric acid secretion was studied in conscious non-vagotomized and in vagotomized gastric fistula dogs. Neither propranolol (0.5 mg/kg) nor practolol (1.0 mg/kg), both intravenously, had a significant influence on basal or histamine-stimulated gastric acid secretion in non-vagotomized and vagotomized dogs. It is concluded that beta-adrenergic blockade has no influence on basal or histamine-stimulated gastric acid secretion in dogs. Furthermore, no differences were found between the non-vagotomized and the vagotomized stomach.

Adrenergic beta-Antagonists↗