Search PubMed⌕ Search

Biomedical subjects

F Galibert

Publications and source records attributed to F Galibert.

At least 217 records · Page 12Linked to original sources
PubMed1971

Evidence for the existence of several molecular species in the "45S fraction" of mammalian ribosomal precursor RNA.

Analysis of RNA (after 5 or 60 min of labeling with [(3)H]uridine) from L5178Y cells by electrophoresis in 1.7% polyacrylamide gels demonstrates that the ribosomal RNA "45S fraction" is not homogeneous but consists of at least three molecular species, conventionally designated 47S RNA, 46S RNA, and 45S RNA. Alternatives to the classical scheme for the biosynthesis of ribosomal RNA in mammalian cells are discussed.

Autoradiography↗
PubMedDate not supplied

Nucleotide sequence analysis of a cloned duck beta-globin cDNA.

The primary structure of the duck beta-globin mRNA was obtained from sequence analysis of the double-stranded in vitro-transcribed DNA cloned in plasma pBR322. The 646-bp long globin DNA insert comprises a coding sequence of 438 bp corresponding to 146 amino acids, a 5'-noncoding region 63 bp long, and a 3'-noncoding region of 113 bp prior to a stretch of adenosine residues. The salient features of each of these regions are discussed and compared with beta-globin mRNAs of other vertebrates.

Animals↗
PubMedDate not supplied

Methylation process of mammalian ribosomal precursor RNA.

The analysis of RNA from L5178 Y cells, by electrophoreis in 1.7% polyacrylamide gel, after short time labelling with [methyl-3H] methionine, shows that the three molecular species 47S, 46S and 45S RNA from the ribosomal RNA "45S fraction" are methylated. Comparative studies of the kinetics obtained after labelling for short periods with [methyl-3H] methionine and [3H] uridine, and brief labelling experiments with [methyl-3H] methionine in the presence of actinomycin D, show that the methylation of ribosomal precursor RNA occurs after the transcription process has been completed, and that methylation and transcription are not coordinated. These results lead us to propose the following scheme for ribosomal RNA biosynthesis : 1) transcription of ribosomal genes, 2) methylation of 47S RNA (and possibly 46S RNA), 3) maturation of these molecules which are converted to 45S RNA.

Cell Line↗