Search PubMed⌕ Search

Biomedical subjects

F Gabrielli

Publications and source records attributed to F Gabrielli.

At least 91 records · Page 5Linked to original sources
PubMedDate not supplied

A multicenter investigation of consultation-liaison psychiatry in Italy. Italian C-L Group.

In order to evaluate the extent and quality of consultation-liaison (C-L) activity in Italy, a multicenter investigation was conducted in 17 general hospitals. All of the hospitalized patients referred to C-L psychiatry during a 1-year period were assessed by means of a specific instrument (Patient Registration Form, PRF-SF). Of 518,212 patients, 4182 were referred to C-L services (referral rate = 0.72%). Typical consultations were for female patients (60.1%), admitted to medical wards (71.5%), aged 55-75 years. Most interventions were carried out within 2 days; a minority (22%) were urgent requests. Gastrointestinal and cardiovascular disorders, and unexplained medical symptoms were the most frequent ICD-9 somatic diagnoses at admission. One-third of the patients were not informed of having been referred to C-L and half of them had a lifetime history of psychiatric disturbances. Most frequent ICD-10 psychiatric diagnoses were neurotic, stress-related, and somatoform syndromes (33.1%), affective syndromes (19.4%), and organic mental syndromes (10.7%). Two-thirds of the patients were given only one consultation whereas the reminder received two to four follow-up visits. The rate of transfer to psychiatric wards was low (2.1%). Psychopharmacological treatment was suggested in 65% of cases, and 75.5% of the patients were referred to community psychiatric care at discharge. The implications of the findings are discussed.

Adolescent↗
PubMedDate not supplied

Sentinel node biopsy for malignant melanoma: analysis of a four-year experience.

AIMS AND BACKGROUND: Sentinel node (SN) biopsy has been introduced to solve the controversy concerning the effectiveness of prophylactic lymphadenectomy in intermediate thickness melanoma. The aim of this study was to evaluate the rate of metastases, the technical details of the procedure, and the main reasons of failure. METHODS: 235 patients affected by intermediate thickness melanoma (tumor thickness >0.75 mm and <4 mm) without clinical signs of systemic spread (N0M0) were submitted to sentinel node biopsy between 1996 and 2000. Preoperative lymphoscintigraphy was routinely performed in the last 184 patients. Intraoperative mapping with gamma probe was combined with the use of vital dye for identification of sentinel nodes in the last 113 patients. RESULTS: The SN detection rate was 95.6%, with significant differences depending on the site of dissection and the use of a gamma probe. The overall rate of micrometastases was 14.7%, but relevant differences were recorded between different subgroups of patients (T2, 5.1%; T3a, 19.6%; T3b, 29%). CONCLUSIONS: Sentinel node biopsy requires a multidisciplinary approach (surgery, pathology and nuclear medicine) for reliable results. The association of vital dye and intraoperative gamma probe for sentinel node harvesting has made the procedure more effective, less time-consuming and less invasive. Failures may be due not only to surgical mistakes, but also to improper nuclear medicine procedures or inaccurate histological evaluation of SNs. Methods for histological examination of the SN are still debated and not standardized but promising results have recently been obtained with molecular oncology techniques (RT-PCR).

Axilla↗
PubMedDate not supplied

Lymphatic mapping and sentinel node identification in squamous cell carcinoma and melanoma of the head and neck.

AIM: The aim of our study was to evaluate the role of scintigraphy in lymphatic mapping and in the identification of the sentinel lymph node (SLN) in patients with head and neck cancer. METHODS: Between September 1999 and February 2001 we enrolled 22 consecutive patients with cancer in the head and neck region: five squamous cell carcinomas, one Merkel cell tumor of the cheek, and 16 malignant melanomas. Lymphoscintigraphy was performed three hours before surgery after injection of 30-50 MBq of 99mTc -Nanocoll in 0.3 mL; the dose was fractionated by injecting the radiotracer at two points around the lesion. Static acquisition (anterior and/or lateral views, 512 x 512 matrix, 5 mins pre-set time) was started immediately after the injections so as to visualize the pathways of lymphatic drainage. The skin projection of the SLN was marked with ink. Intraoperative SLN detection was performed with perilesional injection of patent blue. RESULTS: SLNs were found with lymphoscintigraphy in all patients. Thirty-three SLNs were identified: one occipital node, three nodes at the base of the tongue, 10 superficial lateral nodes (external jugular), five submandibular nodes, five submental nodes, three mastoid nodes and six supraclavicular nodes. Biopsy was performed in 21/22 patients. In 20/22 patients the first lymph nodes were visualized in the proximal cranial regions (retroauricular, jugular and submandibular) at five minutes post injection. The SLN positivity rate was 13.6% (three patients). All patients with tumor-positive SLNs were submitted to radical dissection. Poor concordance in the detection of sentinel nodes was observed with patent blue. CONCLUSIONS: The flow of nanocolloid in the lymph vessels of the head is rapid. In our experience immediate scintigraphic imaging was essential to visualize the pathways of lymphatic drainage and the first SLN. Radioguided SLN biopsy is therefore recommended within three hours. Injection of patent blue is inadvisable because of the poor concordance with lymphoscintigraphy and the risk of permanent tattooing of the face.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Histones of human lung carcinoma and human lung tissue. Analysis by two-dimensional electrophoresis.

Histones prepared from human lung and human lung carcinoma were analyzed by a 2-dimensional electrophoresis method able to separate histone subfractions. The comparative analysis of electrophoretograms showed differences in the relative amounts of H1, H2A, and H3 histone subfractions extracted from human lung and human lung carcinoma chromatin.

Electrophoresis, Polyacrylamide Gel↗
PubMedDate not supplied

[Chromosomal proteins: histones and acid proteins].

Experimental data about the chemistry and the biology of chromosomal proteins are reviewed. Paragraphs include: aminoacid sequential data and post-translational covalent modications of histones, histone chemical differences in different tissues of the same species and in homologous organs of different species, histone synthesis subcellular localization and its association with DNA synthesis, histone synthesis transcriptional and translational control, histone synthesis during meiosis, oogenesis and early embryogenesis. The possible role of histones as controllers of gene expression is discussed and a model of primary structure of chromatine is proposed. The "acidic proteins" data concern the high tissue eterogenity of these proteins and their role in the steroid-hormon-controlled gene expression. The possible role of acidic proteins as general controllers of gene expression in eucariotic cells is discussed.

Acetylation↗
PubMedDate not supplied

[Current approaches in the treatment of gastrinoma metastases].

In the last years the therapeutic behaviour with regard to gastrinomas, has deeply changed becoming more and more aggressive. Nowadays, almost all authors recommend, after a serious treatment of the hypersecretion with omeprazole, an explorative laparatomy in all patients affected by gastrinoma even without a preoperative tumor localization. The multiple endocrine syndrome (MEN I) and the metastatic disease are excluded from the above practice. A careful examination of the most recent data available today in literature accompanied with our experience, even if limited, shows that only the surgical treatment allows a better survival also in metastatic disease. The surgical treatment is surely recommended in gastrinoma with pancreatic and duodenal lynphonodal metastasis. For hepatic metastasis all types of hepatic resection, the "wedge resection" included, can be suggested. After the first positive experiences, the hepatic transplantation can find a place among the therapeutic means against this kind of tumors.

Gastrinoma↗
PubMedDate not supplied

The phosphorylation of nuclear proteins in normal and transformed cells.

Normal (Rat 1) and transformed cells (middle T antigen-transformed derivative 3C3) were grown in the presence of 32P-orthophosphate. 32P-labelled nuclear proteins were fractionated by means of two-dimensional gel electrophoresis and detected by autoradiography. The comparative analysis of the autoradiographs of the normal and transformed cells revealed differences in the phosphorylation patterns of histone and low-molecular-mass high mobility group proteins (HMG). Three of the HMG proteins were highly phosphorylated in the transformed cells, and the analysis of their phosphorylation sites showed that these HMG proteins were phosphorylated on serine and threonine but not on tyrosine residues.

Animals↗
PubMedDate not supplied

[Comparative study of the capacity of elastomers in reproduction and transmission of details to gypsum models].

The authors studied the ability of three elastomeric impression materials in reproducing and transmitting three grooves, engraved in the polished surface of a stainless steel model, to gypsum models. The grooves were 17, 21 and 25 micrometers wide, and the fails in reproducing, as well as in transmitting the minor details to gypsum, were also investigated as to the effects of gypsum particle size, proportional relation water/powder and plasticity of the mixture. The three elastomeric impression materials used were a polysulfide, a silicone and a polyether, and the six gypsum products were two improved stones type IV, two dental stones type III and two dental plasters type II. Scores 3, 2 and 1 were attributed respectively to the 17, 21 and 25 micrometers grooves reproduced in the molds, as well to the same grooves transmitted to the gypsum models. The results showed that all the impression materials tested were able to reproduce in the molds all the three grooves present in the steel model. Nevertheless, when gypsum models were examined, it was verified that the grooves so well reproduced in the molds were neither equally nor uniformely registered in the respective models, the best results being achieved by the polysulfide and the silicone, an the worst by polyether, As to the gypsum products, the type II gypsum presented the most unfavorable results.

Calcium Sulfate↗
PubMedDate not supplied

[Echocardiography in the diagnosis of rupture of chordae tendineae. Description of a clinical case].

A case of ruptured chordae tendineae is reported. The patient, a 53 year old man, had a crescendo-decrescendo holosystolic murmur, a third and a fourth heart sound, that is the typical auscultatory pattern of this lesion. However, M-mode and 2-D echocardiography had a permanent role in arriving at the diagnosis. M-mode echocardiography, in fact, showed a clear systolic flutter of the mitral valve and a large anterior diastolic motion of the posterior mitral leaflet; 2-D echo, besides, revealed a quick systolic motion into the left atrium of the tip of the posterior mitral leaflet and lack of coaptation of mitral leaflet during systole. The patient underwent an operation of valve repair. At the postoperative echocardiographic examination none of the features of flail posterior mitral leaflet were found. This fact can demonstrate the success of the operation.

Chordae Tendineae↗