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Biomedical subjects

F Gabrielli

Publications and source records attributed to F Gabrielli.

At least 55 records · Page 3Linked to original sources
PubMed1986

H1(0) histones of normal and cancer human cells. Amino acid composition of H1 purified by polyacrylamide gel electrophoresis.

H1 histones were purified by preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis from human lung carcinoma (line DMS79), human hepatoblastoma (HepG2), human adult lung and human adult and fetal liver. The purified human H1 histones were analyzed for their amino acid composition and terminal residues. The comparative analysis of the amino acid compositions of the different human H1 histones showed that: all the H1 preparations have the characteristically high lysine content associated with a low arginine content, which distinguishes outer histones from core histones; H1 is distinguishable from other H1 histones by the presence of methionine and histidine; H1 histones from human adult, fetal and cancer cells are very similar in amino acid composition, and in cancer cells the level of the H1 histone is not inversely related with cell growth rate nor with the expression of the alpha-fetoprotein gene.

Amino Acids↗
PubMed1984

[Needle aspiration cytology in the diagnosis of thyroid nodules].

Routine cytological fine needle aspiration was introduced in cases where scintigraphy and echography revealed cold, solid thyroid nodules. The cytological patterns of goitre, thyroiditis and neoplasias are briefly described. Needle aspiration cytology appears to provide substantial diagnostic accuracy with a low incidence of false positives or negatives. This examination is essentially risk-free, and easy to perform, providing fast, accurate diagnosis and making surgery superfluous.

Adenoma↗
PubMed1984

Histone complements of human tissues, carcinomas, and carcinoma-derived cell lines.

The pattern of subtypes of the nucleosomal histones and of histone H1 was investigated in human cells from adult and fetal lung and liver, from carcinoma tissues and from carcinoma-derived cell lines, with the object of comparing these patterns, and their relationship to cell growth rate, with those in cells of other species. The subtype pattern of the nucleosomal histones H2A and H3 shows a correlation with replication rate. In adult tissues, subtype H3-3 predominates over H3-2 and H3-1, and the subtype H2A-1 and H2A-2 are approximately equally abundant. In fetal tissues, lung carcinoma and cultured carcinoma-derived cell lines, the subtype H3-1 is predominant and H2A-1 is more abundant than H2A-2. The subtype pattern of H1 also differs between normal and carcinoma cells, among different tissues, and in different cell lines derived from the same type of carcinoma. In particular, the relative level of H1 degrees differs in several cell lines showing relatively high rates of replication, and in some cases represents more than 25% of the total H1, similar to the level in slowly replicating normal adult liver and lung tissues. The relative level of H1 degrees does not therefore appear to be correlated in a simple manner with cell growth rate in these human cells.

Animals↗
PubMed1983

Minireview. Roles of turnover and repair of macromolecules and supramolecular structural components.

Macromolecules and supramolecular structural components that are incorrectly synthesized or are damaged by radiation or by reactive chemicals are either repaired or selectively degraded and resynthesized. In addition, turnover rates for macromolecules and supramolecular structures can be elevated by alternation of fasting and feeding periods and can be influenced by metabolic regulatory mechanisms which are governed by steady-state concentrations of labile macromolecules.

Animals↗
PubMed1983

[Therapeutic use of flunarizine in the treatment arteriopathies of the lower extremities].

94 patients with Stage II obliterating arteriopathy of the lower extremities, treated with Flunarizine (10-20 mg per diem), were checked every two months for a 6 month period. Of the 76 patients reaching the final check up, 58 had received no previous treatment and 18 had ischemias secondary to reconstructive vascular surgery. Clinical improvement in terms of increased independent mobility and improved Doppler flowmeter pressure readings were noted in both groups. In view of its beneficial effects on blood vessel function in other areas, Flunarizine is felt to be a valuable drug for the treatment of peripheral arteriopathies, both initially and in cases of ischaemia following vascular surgery.

Aged↗
PubMed1983

[Emergency situations in pathology of the left colon: validity of fibrosigmoidoscopy in differential diagnosis].

The Authors, although not working in an emergency ward, have performed 30 fiberoptic sigmoidoscopies (FFS) in emergencies on patients hospitalized in their Institutes. The indications for the investigation were: acute bowel obstruction (22 cases); severe post-operative diarrhea (5 cases); massive rectal bleeding (8 cases). In all the patients, FFS, performed immediately, allowed diagnosis of both the type and the site of tht lesion. The Authors believe that fiberoptic sigmoidoscopy, in trained hands, in emergencies can be a valid and safe tool for a quick and sure diagnosis. In particular, as far as acute intestinal obstruction is concerned, FFS can prove useful for the defferential diagnosis between mechanical and dynamic ileus; it is also clearly indicated in order to establish, during the pre-operative phase, the neoplastic or not-neoplastic character of the lesions. FFS should, therefore, be performed every time the clinical data and the direct radiography of the abdomen suggest a left colon obstruction.

Acute Disease↗
PubMed1981

[Bilateral carcinoma of the breast].

In the light of literature and personal experience the Authors analyse the problem of bilateral breast cancer. Distinction between primary cancer (synchronous or metachronous) and metastasis in the second breast is made on the bases of clinical and histological criteria. Lobular cancer, either in situ of infiltrating, has a higher incidence of bilaterality. Aethiology, incidence, prognostic significance of the second cancer are briefly discussed; particularly, it is emphasized that there is a "high risk" group of patients in which the incidence of primary contralateral cancer is much higher. The Authors stress the importance of clinical examination of the other breast at the time of initial mastectomy for a proven breast cancer. The risk of metachronous cancer may be prevented by prophylactic contralateral mastectomy in the "high risk" patients or by random biopsy of the opposite breast in all the patients undergoing surgical therapy for cancer in the first breast. It is opinion of the Authors that early diagnosis may be also obtained if the patients are carefully followed by regular physical examinations and by diagnostic aids (mammography, ultrasonography, thermography).

Breast Neoplasms↗
PubMed1981

Polysomal and nonpolysomal messenger RNA of noninduced and induced Friend erythroleukemic cells: analysis by cell-free translation.

Polyadenylated and nonpolyadenylated mRNA were prepared from polysomes and from the postribosomal supernatant of noninduced and DMSO-induced Friend cells. The mRNA preparations were translated in a wheat germ cell-free system and the in vitro synthesized proteins, fractionated by polyacrylamide gel electrophoresis, were compared by fluorography. The electrophoretic analysis shows that four preparations of poly (A) + RNA code for many different peptides and that most of these peptides are present in each of the poly (A) + RNA translation products. However, the electrophoretic patterns of these translation products differ in the relative amounts of peptides comigrating in the gel electrophoresis. After DMSO treatment, Friend cells show significative differences in the polysomal and nonpolysomal mRNA pools. With induction, globin becomes the most abundant product of the polysomal poly (A) + RNA, while the relative amounts of peptides coded by nonglobin polysomal poly (A) + RNA are reduced. In parallel, the electrophoretic pattern of the in vitro products on the nonpolysomal poly (A) + RNA changes in the relative amounts of the fractionated peptides; moreover, in induced cells, the nonpolysomal poly (A) + RNA codes for peptides not detected in the polysomal poly (A) + RNA of the same cells. These data were interpreted assuming that in DMSO-induced cells, protein synthesis is regulated at both the transcriptional and translational levels. Polysomal poly (A)-RNA codes mostly for the five main histone classes; with DMSO treatment the amount of H2b MRNA bound to polysomes is increased with respect to the other polysomal histone mRNA.

Animals↗
PubMed1981

Characterisation of a chromatin fraction bearing pulse-labelled RNA. 2. Quantification of histones and high-mobility-group proteins.

The histone variants and high-mobility-group (HMG) proteins of a transcribing fraction of chromatin, described in the preceding paper of this journal, have been analysed qualitatively and quantitatively by a combination of one-dimensional and two-dimensional gel electrophoresis. The stoichiometry of the four core histones (all variants included) in this fraction is equimolar and is not detectably different from that in the nontranscribing fraction or in total chromatin. The molar ratio of histone H1 to the core histones is markedly lower, by approximately 72%, than that in the nontranscribing fraction. A minor histone variant identified as M1 (an H2A variant) is detected only in the transcribing fraction, while variant H3.1 is found only in the non-transcribing fraction. Proteins A24, HMG1 and HMG2 are essentially absent from the transcribing fraction; HMG14 is found uniquely in this fraction, while HMG17 occurs at a relatively lower level.

Animals↗