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Biomedical subjects

F E Bloom

Publications and source records attributed to F E Bloom.

At least 415 records · Page 23Linked to original sources
PubMed1972

Adenosine 3',5'-monophosphate is localized in cerebellar neurons: immunofluorescence evidence.

Adenosine 3',5'-monophosphate is localized in specific cerebellar neurons, as shown by fluorescence immunocytochemistry with a specific rabbit immunoglobulin. Positive staining is exhibited by Purkinje neurons and granule cells. The increase in concentration of cyclic adenosine monophosphate in the cerebellum, which is known to follow decapitation, is represented by greatly increased fluorescence of Purkinje neurons only. These immunofluorescence data provide the first evidence for localization of cyclic adenosine monophosphate in specific neurons and may permit further exploration into the role of this cyclic nucleotide in neuronal function.

Animals↗
PubMed1972

GABA catabolism: localization of succinic semialdehyde dehydrogenase in brain motor and sensory nuclei.

The localization of brain succinic semialdehyde dehydrogenase, a specific gamma-aminobutyric acid degradative enzyme, could potentially yield valuable information concerning the function of the enzyme. Application of a new histochemical technique for this enzyme has revealed characteristic patterns of neuronal staining that are contsistent within embryologically and functionally similar nuclei of the brainstem of the rat.

Aldehydes↗
PubMed1972

Osmiophilic polymer generation: catalysis by transition metal compounds in ultrastructural cytochemistry.

A transition metal compound that is bound in tissues by any appropriate cytochemical reaction may catalyze the generation of an insoluble osmiophilic polymer from organic monomers such as 3,3'-diaminobenzidine. When the polymers are treated with osmium tetroxide, electron-opaque, insoluble osmium blacks (coordination polymers of osmium) are formed at the sites of the particular macromolecule or enzyme permitting its light, and electron, microscopic localization. This approach represents a distinct advantage over earlier cytochemical methods because the shorter incubation time needed here results in less artifactual deposition of metal ions, and less tendency to crystallize the reaction product. In addition, the shorter incubation times permit longer fixation of tissues and hence less artifact due to enzyme diffusion.

Acid Phosphatase↗
PubMed1972

Axonal sprouts and ( 3 H)norepinephrine uptake by superior cervical ganglia in organ culture.

Superior cervical ganglia from adult rats maintained in organ culture show a progressive increase in the rate of uptake of [(3)H]norepinephrine. The enhanced uptake of norepinephrine is a consequence of the development of axonal sprouts. Formation of axonal sprouts, which appear to have many of the properties of sympathetic nerve endings, is inhibited by colchicine and vinblastine, presumably because of the interaction of these drugs with neurotubular protein.

Animals↗