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Biomedical subjects

F Denis

Publications and source records attributed to F Denis.

389 records · Page 22Linked to original sources
PubMedDate not supplied

[Place of Staphylococcus aureus in the bacterial isolations of the University Hospital Center of Dakar. Preliminary results of serotyping and phage typing].

Out of 18 837 strains isolated between 1972 and 1980, by the laboratory of bacteriology of the Dakar Teaching Hospital, 2 925 strains of Staphylococcus aureus have been identified. This germ was the cause of 14,4 p. 100 of all diagnosed septicemia, ranging second in frequency after Salmonella. It was the most frequent germ in pura (54,8 p. 100 of strains) and rated 29,2 p. 100 of the strains isolated from pleural fluids. It was less frequent in bacterial meningitidis (2,5 p. 100) and in infections of the urinary tract (3,6 p. 100). About one hundred coagulase + strains were studied further. A clumping factor was found in 98 p. 100 of the strains (Staphyslide test). In addition, serotyping and lysotyping were carried out: --80,8 p. 100 of strains were lysotyped --25,6 p. 100 of them belonging to type I --7,7 p. 100 to type II --10,3 p. 100 to type III --33,3 p. 100 belonging to two or three types --11,5 p. 100 to I and II --16,7 p. 100 to I and III --5,1 p. 100 to I and II and III Some strains gave positive responses with 94-96 and 187 phages.

Bacteriophage Typing↗
PubMedDate not supplied

[Partial purification of the extracellular proteolytic system of Aeromonas hydrophila LP50: comparative chromatographic and electrophoretic study].

A complex extracellular proteolytic system was produced by Aeromonas hydrophila LP50 on glucose- polypeptone medium. Partial purification of this system was accomplished by ammonium sulphate precipitation, acetone precipitation, gel filtration on Sephacryl- S200 and chromatography on DEAE-Sephacel. Every stage was controlled by electrophoresis. This proteolytic system was constituted of three aminopeptidase and two endopeptidase components.

Aeromonas↗
PubMedDate not supplied

[Vaccination against hepatitis B virus. Value of intradermal administration in dialysed patients non responsive to intramuscular approach].

OBJECTIVES: Approximately 16-27% of dialysis patients (DP) have no detectable antibodies after 5 intramuscular injections of hepatitis B vaccine and represent a group at high risk to contract hepatitis B virus. We report the efficacy of the intradermal route of a recombinant hepatitis B vaccine (r-HBV) in non-responsive dialysis patients in our dialysis unit. METHODS: Intradermal vaccinations were performed in 20 dialysis patients (mean age 62 years) non-responsive to the intramuscular injections (mean 6.8). Five micrograms of r-HBV (Engerix B, SK and F) were administered intradermally every two weeks (maximum 70 micrograms) until a level of anti-HBV antibodies (anti-HBs) arbitrarily choosen of > or = 230 mUI/ml was attained. Anti-HBs was determined after the fourth and subsequent intradermal injections (IMX, Abbott). RESULTS: Fourteen dialysis patients (70%) developed anti-HBs > 10 mUI/ml (geometric mean titers of 330 mIU/ml). Among these, 9 developed seroprotective levels before the fifth injection. Five patients developed anti-HBs > or = 1000 mUI/ml and 6 others developed anti-HBs > or = 230 mUI/ml. After the intradermal injections were discontinued, 11 patients were monthly monitored for at least 3 months, and 6 for one year. The geometric mean antibody level was at 3 months: 157 (n = 11), at 6 months: 122 (n = 8), at nine months: 117 (n = 6), and at 12 months: 66 mIU/ml (n = 6). The age and the sex, haemodialysis duration, albumin levels or treatment by erythropoietin did not seem to play a role in appearance of anti-HBs. CONCLUSIONS: Our experience in 20 dialysis patients shows that repeated low-dose intradermal injections resulted in long-term seroprotection in a substantial number of dialysis patients non-responsive to the intramuscular vaccinations.

Adult↗
PubMedDate not supplied

Complications of anterior spinal surgery in children.

The purpose of this study was to document the medical and surgical complications of anterior spine surgery in children and to identify risk factors for complications. A retrospective chart review was conducted of 599 anterior procedures (24 anterior only, 300 staged anterior/posterior, 175 combined anterior/posterior procedures) performed between 1967 and 1991. Major complications occurred in 7.5% of procedures and minor complications in 33%. Risk factors for major complications were age > 14 years, male gender, kyphotic curve type, curve sizes > 100 degrees, vital capacity < 40% of predicted, and use of thoracotomy. Risk factors for minor complications were age > 14 years, curves > 100 degrees, vital capacity < 40% of predicted, and use of a staged procedure. Multivariate analyses of risk factors identified age > 14 years and curves > 100 degrees as the most significant risk factors for major complications and age > 14 years for minor complications. We concluded that anterior spinal surgery can be performed in children with an acceptable level of risk and that referral for surgery before 14 years of age and before the curve size progresses will significantly reduce the risk of complications.

Adolescent↗
PubMedDate not supplied

[Virus and gastrointestinal infections].

Numerous viruses found in the gut are not associated with primary infection or disease of the gastrointestinal tract. Other groups or viruses are not classically associated with infection of the gut but can infect the gastrointestinal tract in immunocompromised individuals (herpes simplex virus, cytomegalovirus, papillomavirus ....). The viruses associated with gastroenteritis represent a large number of taxonomic group. Because these viruses have in general been difficult to cultivate, most members of this group were firstly detected by electron microscopic examination (adenovirus, astrovirus, calicivirus, coronavirus, rotavirus ....). The most widely used diagnostic techniques for adenovirus (40/41), rotavirus and astrovirus detection in faecal samples include immunoassays such as Elisa and latex agglutination. Nucleic acid hybridization techniques have generally not proven to be substantially sensitive and the more sensitive techniques recently developed use the polymerase chain reaction (adenovirus) or the reverse transcription/polymerase chain reaction (astrovirus, calicivirus, coronavirus, rotavirus). Special efforts have been made in the search for efficient procedures to extract viral nucleic acids, and to establish the optimal conditions for the amplification and identification of PCR products but the candidate viruses were very different, consensus procedures were not determined, and amplification kits were not actually commercialized.

Clinical Laboratory Techniques↗
PubMedDate not supplied

Curve progression in Risser stage 0 or 1 patients after posterior spinal fusion for idiopathic scoliosis.

A retrospective review was performed to determine "crankshaft" prevalence in 86 immature patients who underwent posterior spinal fusion for idiopathic scoliosis. Tanner stage, chronologic age, bone age, and epiphyseal status were used as maturity indicators. Overall, 62 (72%) patients progressed < or = 10 degrees, 18 (21%) patients progressed 11-15 degrees, and six (7%) patients progressed > or = 16 degrees in the coronal plane. Tanner I patients with open triradiate cartilage had the highest rate of crankshaft occurrence; nine (75%) of 12 patients progressed >10 degrees (p < 0.05). Fifty-two percent of Tanner I, 26% of Tanner II, 11% of Tanner III, and no Tanner IV patients progressed >10 degrees (p < 0.05). Cobb angle increases of >10 degrees degrees occurred in 54% of patients with open triradiate cartilage (p < 0.05) and in 48% of patients with open capital femoral epiphyses (p < 0.05). Anterior and posterior spinal fusion should be considered in prepubertal (Tanner I) patients with open triradiate cartilage.

Adolescent↗
PubMedDate not supplied

Spinal instrumentation without fusion for progressive scoliosis in young children.

Between 1973 and 1993, a heterogeneous group of 67 children with progressive scoliosis entered a program of incremental-distraction spinal instrumentation without fusion supplemented by full-time external orthotic support. Over the course of treatment, curve magnitude improved from an average of 67 degrees at initial instrumentation to 47 degrees at definitive fusion. For all patients, curve response tended to decline with consecutive procedures. The measured growth of the instrumented but unfused spinal segments averaged 3.1 cm over a mean treatment period of 3.1 years. The results of our study suggest that spinal instrumentation without fusion can control progressive scoliosis in a majority of children while allowing normalized growth of instrumented spinal segments. The mean duration of treatment and ultimate gain in spinal length are constrained by progressive structural changes that alter curve response to incremental distraction. Despite these limitations, spinal instrumentation without fusion may provide a reasonable management alternative when individualized among these difficult patients.

Adolescent↗